Rabbit anti Human cyclin dependent kinase 4
Catalogue Number: X1216P-EXA
| Manufacturer: | ExAlpha |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | TrpE-fusion protein containing the C-terminal 164 amino acids of human Cdk4 |
| Application: | IP, WB |
Description
Description: p34cdk4 is a major catalytic subunit of mammalian D-type cyclins which is expressed by a gene found in human chromosome 12q13. The cdk4 protein is constitutively expressed throughout the cell cycle and it is the assembly and accumulation of functional cdk4/cyclin D complexes which is rate limiting for the progression through the G1 phase of the cell cycle. Once the complex is formed it must be activated by the phosphorylation of threonine 172 on the cdk4 subunit, a reaction mediated by a cdk-activating kinase (CAK). Complex formation and phosphorylation are independently regulated events. The phosphorylation of the cdk4 subunit is subject to regulation of both stimulatory and inhibitory cytokine signals, thereby allowing cdk4 to function as a mid G1 checkpoint. Independent of phosphorylation, cdk4 is also regulated by the binding of p16Ink4/mts1 while in complex with cyclin D.
Additional Text
Gene Name
CDK4
Gene ID
1019
Uniprot ID
P11802
Purification
Ammonium Sulfate Precipitated
Antibody Clonality
Polyclonal
Application Notes
Detects Cdk4 by Western blot in HeLa cell lysates. Can also be used for immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.
Storage Note
Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
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