Mouse anti Human Keratin 14

Catalogue Number: X1253M-EXA

Manufacturer:	ExAlpha
Type:	Monoclonal Primary Antibody - Unconjugated
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Mouse
Clone:	DE-SPK14
Isotype:	IgG2b
Immunogen:	Hybridoma produced by the fusion of splenocytes from mice immunized with synthetic peptide conjugated to KLH corresponding to the carboxy terminal sequence of human cytokeratin 14 (KVVSTHEQVLRTKN) and mouse myeloma cells.
Application:	WB, IHC-Fr

Overview

Description

Description: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. This antibody reacts specifically with human keratin 14 by immunoblotting. In tissue sections, it provides a positive reaction on basal cells of non-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis and gingiva, myoepithelial cells and squamous cell carcinomas.

Additional Text

Gene ID

3861

Gene Name

KRT14

Uniprot ID

P02533

Purification

Protein A/G Purified

Antibody Clonality

Monoclonal

Application Notes

Detects human cytokeratin 14 by Western blot at 50 kDa. Does not react with other cytokeratins or other proteins. Also works for immunohistochemistry using frozen tissues. Does not work with paraffin embedded tissues. Optimal concentration should be evaluated by serial dilutions.

Storage Note

Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles

Price

£322.00

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