Mouse anti Human Keratin 5 & 8

Catalogue Number: X1257M-EXA

Manufacturer:	ExAlpha
Type:	Monoclonal Primary Antibody - Unconjugated
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Mouse
Clone:	C50
Isotype:	IgG1
Immunogen:	Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation of HeLa cells and mouse myeloma cells.
Application:	WB, IHC-Fr

Overview

Description

Description: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Simple epithelia express cytokeratin 8 in combination with 18. Basal cells of stratified epithelia express cytokeratin 5 with cytokeratin 14. Therefore this antibody reacts with a wide range of epithelia and their carcinomas.

Additional Text

Gene Name

KRT8

Gene ID

3856

Uniprot ID

P05787

Purification

Protein A/G Purified

Antibody Clonality

Monoclonal

Application Notes

Detects Keratin 5 & 8 by Western blot. Detects a 58 kDa band, corresponding to Keratin 5, and a 52.5 kDa band, corresponding to Keratin 8. Optimal concentration should be evaluated by serial dilutions.

Storage Note

Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles

Price

£322.00

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