N-cadherin Polyclonal Antibody
Catalogue Number: BS-1172R-BSS
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bs-1172R Image
bs-1172R Image
bs-1172R Image
bs-1172R Image
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
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Formalin-fixed and paraffin embedded rat brain labeled with Anti-CDH2/N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
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Formalin-fixed and paraffin-embedded rat brain labeled with Anti-CDH2/N-cadherin Polyclonal Antibody, Unconjugated(bs-1172R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.
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Mouse breast cancer lysates probed with Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:300 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CDHN; NCAD; CD325; CDw325; Cadherin-2; Neural cadherin; N-cadherin; CDH2; CADH2_HUMAN |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human N-cadherin |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).
Additional Text
Gene Name
CDH2
Gene ID
1000
Uniprot ID
P19022
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1172R Datasheet
SUPPORT
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DELIVERY
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