CX3CR1 Polyclonal Antibody
Catalogue Number: BS-1728R-BSS
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Lane 1: Mouse Heart lysates; Lane 2: Rat Heart lysates probed with CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Lung lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Mouse Spleen cell lysates probed with CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Raw264.7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CX3CR1 Polyclonal Antibody(bs-1728R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CX3CR1) Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Lane 1: Mouse Spinal cord tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Spinal cord tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Mouse splenocytes probed with Rabbit Anti-CX3CR1 Polyclonal Antibody, FITC Conjugated (bs-1728R-FITC) (green) at 1:100 for 40 minutes compared to control cells (blue).
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Lane 1: rat brain lysates Lane 2: mouse heart lysates probed with Anti CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 39kD. Observed band size: 39kD.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | V28; CCRL1; GPR13; CMKDR1; GPRV28; CMKBRL1; CX3C chemokine receptor 1; C-X3-C CKR-1; CX3CR1; Beta chemokine receptor-like 1; CMK-BRL-1; CMK-BRL1; Fractalkine receptor; G-protein coupled receptor 13 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human CX3CR1 |
| Application: | ELISA, FC, IHC-P, WB, IHC-Fr |
Description
Description: Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
Additional Text
Gene ID
1524
Gene Name
CX3CR1
Uniprot ID
P49238
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1728R Datasheet
SUPPORT
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