APG5L Polyclonal Antibody
Catalogue Number: BS-4005R-BSS
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Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti-APG5L/ATG5 Polyclonal Antibody (bs-4005R) at 1:100 followed by conjugation to the secondary antibody and DAB staining.
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Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-APG5L/ATG5 Polyclonal Antibody (bs-4005R ) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with APG5L Polyclonal Antibody, Unconjugated (bs-4005R) at 1:200 overnight at 4°C, DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APG5L) Polyclonal Antibody, Unconjugated (bs-4005R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
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Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Rat Cerebrum tissue lysates probed with ATG5/APG5 Polyclonal Antibody, Unconjugated (bs-4005R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti APG5L/ATG5 Polyclonal Antibody, Unconjugated (bs-4005R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band 32kD. Observed band size: 32kD.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | ASP; APG5; APG5L; hAPG5; APG5-LIKE; Autophagy protein 5; Apoptosis-specific protein; ATG5 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human APG5L |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG1 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT2 via the autophagic pathway. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.
Additional Text
Gene Name
ATG5
Uniprot ID
Q9H1Y0
Gene ID
9474
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-4005R Datasheet
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