Cytochrome C Recombinant Antibody

Catalogue Number: BSM-52050R-BSS

BSM-52050R Image

MCF-7 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

BSM-52050R Image

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52050R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney tissue incubated with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52050R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52050R Image

Paraformaldehyde-fixed and paraffin-embedded Rat kidney tissue incubated with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52050R Image

Paraformaldehyde-fixed and paraffin-embedded Rat liver tissue incubated with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytochrome C (1F10) Monoclonal Antibody, Unconjugated (bsm-52050R) at 1:200 overnight at 4°C, DAB staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytochrome C (1F10) Monoclonal Antibody, Unconjugated (bsm-52050R) at 1:200 overnight at 4°C, DAB staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytochrome C (1F10) Monoclonal Antibody, Unconjugated (bsm-52050R) at 1:200 overnight at 4°C, DAB staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytochrome C (1F10) Monoclonal Antibody, Unconjugated (bsm-52050R) at 1:200 overnight at 4°C, DAB staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytochrome C (1F10) Monoclonal Antibody, Unconjugated (bsm-52050R) at 1:200 overnight at 4°C, DAB staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytochrome C (1F10) Monoclonal Antibody, Unconjugated (bsm-52050R) at 1:200 overnight at 4°C, DAB staining.

BSM-52050R Image

Lane 1: Mouse Heart lysates; Lane 2: Rat Heart lysates; Lane 3: Mouse Kidney lysates; Lane 4: Rat Kidney lysates; Lane 5: Mouse Muscle lysates; Lane 6: Rat Muscle lysates; Lane 7: Mouse Liver lysates; Lane 8: Rat Liver lysates; Lane 9: Mouse Cerebrum lysates; Lane 10: Rat Cerebrum lysates; Lane 11: Human Jurkat cell lysates probed with Cytochrome C (1F10) Monoclonal Antibody, Unconjugated (bsm-52050R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-52050R Image

Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Kidney tissue lysates; Lane 5: Rat Muscle tissue lysates probed with Cytochrome C Monoclonal Antibody, Unconjugated (bsm-52050R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Rat Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytochrome C Monoclonal Antibody, Unconjugated(bsm-52050R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52050R Image

25 ug total protein per lane of various lysates (see on figure) probed with Cytochrome C monoclonal antibody, unconjugated (bsm-52050R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

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Manufacturer:	Bioss Inc
Type:	Recombinant Monoclonal
Alias:	CYC; HCS; THC4; Cytochrome c; CYCS
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Recombinant human Cytochrome C protein, full length.
Application:	ICC, IF, IHC-P, WB, IHC-Fr

Overview
Datasheets

Description

Description: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.