The Cold Fusion technology is a simple, rapid and highly efficient cloning kit. It allows you to directly clone any PCR product(s) to any linearized expression vector, at any site. The PCR fragments can be generated by Taq DNA polymerase or other high fidelity DNA polymerases, with primers that are designed to have at least 15 bases of homology at their linear ends to where you want the product to “fuse”. The linearized vector can be generated by PCR or restriction enzymes (single or double cut). One tube simple reaction format, 5 minute incubation at room temperature then 10 minutes on ice, your PCR product(s) rapidly and accurately fuse into the linearized vector in the desired orientation. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single step. The system is highly efficient, with more than 95% positive cloning rate.
Cloning has never been so easy
- Clone any fragment into any vector
- Fuse multiple fragments together
- Entire process takes 20 minutes
- Amenable to high throughput cloning applications
Seamless cloning of any DNA fragment into any vector at any location using the Cold Fusion range.
|Cold Fusion Cloning Kit with Competent Cells||10 rxns||MC010A-1-SBI|
|Cold Fusion Cloning Kit with Competent Cells||20 rxns||MC100A-1-SBI|
|Cold Fusion Cloning Kit with Competent Cells||50 rxns||MC101A-1-SBI|
|Cold Fusion Cloning Kit [96X] with 96-well format for Competent Cells||96 reactions||MC096A-1-SBI|