SynVivo Videos

 

Endothelial Cell Division Under Flow

Time lapse video (~24 hours) of primary endothelial cells undergoing cell division and morphological changes under physiological flow conditions in the SynVivo microfluidic, cell-based assay platform.


Leukocyte Interaction with Endothelium in SynVivo Microvascular Network

A wide-field view of SynVivo microfluidic chip showing leukocytes interacting with endothelial cells in a microvascular network comprised of endothelium lined channels and chemo-attractant loaded tissue chamber. Separating the endothelium channel and the tissue chamber is an engineered porous barrier for monitoring leukocyte migration. Endothelial cells have been activated with TNF-alpha to initiate the inflammation response. Leukocyte rolling, adhering and migration across the endothelium can be observed in real-time.


Real-time Single Cell Migration Tracking

High magnification view of a single leukocyte migrating across the engineered barrier separating an endothelial lined channel (left side) from a chemo-attractant laced chamber (right side). Note the extreme shape changes that the leukocyte goes through during the migration process.


Real-time Leukocyte Rolling-Adhesion-Migration Assay

A zoomed in view of SynVivo microfluidic chip showing leukocytes interacting with endothelial cells in a microvasculature sized channel adjacent to a chemo-attractant perfused chamber (left side of image). Separating the endothelium channel and the chamber is an engineered porous barrier for monitoring leukocyte migration. Endothelial cells have been activated with TNF-alpha to initiate the inflammation response. Leukocyte rolling, adhering and migration across the endothelium can be observed in real-time.


3D Tumor Cell Growth


Z-Stack of Endothelial Cells


Leukocytes rolling binding


Open Vasculature


Leukocytes Binding

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