WHICH IS BETTER, PVDF vs. NITROCELLULOSE, in WESTERN BLOT?

Contact UsView All Products Choosing an appropriate membrane for your western blotting (WB) is critical. Among many choices, the most Read More...

Western blotting guide: Part 8, Visualization

Contact Us Visualization of the target proteins is the object of a western blot. There are a number of different Read More...

Western blotting guide: Part 6, Secondary Antibodies

Contact Us The secondary antibody detects the primary antibody, typically conjugated to a reporter molecule it enables the visualization of Read More...

Western blotting guide: Part 5, Primary Antibodies

Contact Us Primary antibodies are used to detect the protein of interest. Part 5 of the Western blotting guide details Read More...

Western blotting guide: Part 4, Membrane blocking

Contact Us Blocking is essential to prevent non-specific interactions between the transferred proteins, the membrane and the subsequent reagents used Read More...

Western blotting guide: Part 3, Electroblotting – Protein Transfer

Contact Us Electrophoresis allows the proteins separated by SDS-PAGE to be transferred from the gel onto a membrane by electrophoretic Read More...

Western blotting guide: Part 2, Protein separation by SDS-PAGE

Contact Us Once prepared, the sample proteins are separated by PAGE (polyacrylamide gel electrophoresis), which may be performed under native Read More...

Western blotting guide: Part 1, Introduction and Sample Preparation

Contact Us In 1979, Towbin et al. first detailed the process of immunoblotting – the separation of proteins from a Read More...