Western blotting guide: Part 6, Secondary Antibodies

Contact Us The secondary antibody detects the primary antibody, typically conjugated to a reporter molecule it enables the visualization of Read More...

Western blotting guide: Part 5, Primary Antibodies

Contact Us Primary antibodies are used to detect the protein of interest. Part 5 of the Western blotting guide details Read More...

Western blotting guide: Part 4, Membrane blocking

Contact Us Blocking is essential to prevent non-specific interactions between the transferred proteins, the membrane and the subsequent reagents used Read More...

Western blotting guide: Part 3, Electroblotting – Protein Transfer

Contact Us Electrophoresis allows the proteins separated by SDS-PAGE to be transferred from the gel onto a membrane by electrophoretic Read More...

Western blotting guide: Part 2, Protein separation by SDS-PAGE

Contact Us Once prepared, the sample proteins are separated by PAGE (polyacrylamide gel electrophoresis), which may be performed under native Read More...

Western blotting guide: Part 1, Introduction and Sample Preparation

Contact Us In 1979, Towbin et al. first detailed the process of immunoblotting – the separation of proteins from a Read More...