Alpha-amylase Kinetic Kit Research

Catalogue Number: 1-1902-SAL

Manufacturer:	Salimetrics LLC
Type:	Human ELISA Kit
Shipping Condition:	Blue Ice
Storage Condition:	4°C
Unit(s):	96 Tests
Range:	N/A
Sensitivity:	N/A
Sample type:	Saliva
Sample size:	10 µL of saliva then 8 µL of X200 dilution
Application:	Biochemical Assay

Overview
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Description

Description: Salimetrics salivary assay kits are expertly designed, developed and validated to ensure accuracy in saliva and proven to deliver precision results for saliva biomarkers. The Salimetrics Salivary Alpha-Amylase Assay Kit is specifically designed to standardize the detection of alpha-amylase in saliva samples for research and biomedical laboratories. α-Amylase has been identified as a biomarker that shows potential to be a surrogate marker of autonomic (sympathetic) nervous system activation. Secretion of α-amylase from the salivary glands is controlled by autonomic nervous signals and a substantial amount of literature reveals that salivary α-amylase is a correlate of sympathetic activity under conditions of stress. Studies show that levels of salivary α-amylase increase under a variety of physically (i.e., exercise, heat and cold) and psychologically (i.e., written examinations) stressful conditions in human subjects. Studies also show that α-amylase levels do not often correlate with cortisol during stress, suggesting that individual differences in α-amylase represent a response to a stress signal independent of the LHPA axis. Alpha-amylase is also believed to play a key role in the establishment and maintenance of the oral microflora to form dental plaques. Although its release from the salivary cells is greatly increased in response to taste or chewing motions of the jaw, salivary α-amylase levels are not related to α-amylase levels in blood, which are derived from pancreatic secretion. In addition, alpha-amylase is present in low levels in non-stimulated saliva between meals, due to its important anti-bacterial role in the oral cavity. Using a small sample volume, this direct saliva assay kit has an extended range which spans the expected alpha-amylase levels found in human saliva. The average inter- and intra-assay precision coefficients of variation are low with no deleterious matrix effects often found in saliva which are characterized through dilution- and spike-recovery validation procedures. This alpha-amylase assay kit has also been formatted to minimize cross reactivity for related proteins.

1-1902 Protocol

Price

£268.00

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