Endothelial Cell Division Under Flow
Time lapse video (~24 hours) of primary endothelial cells undergoing cell division and morphological changes under physiological flow conditions in the SynVivo microfluidic, cell-based assay platform.
Leukocyte Interaction with Endothelium in SynVivo Microvascular Network
A wide-field view of SynVivo microfluidic chip showing leukocytes interacting with endothelial cells in a microvascular network comprised of endothelium lined channels and chemo-attractant loaded tissue chamber. Separating the endothelium channel and the tissue chamber is an engineered porous barrier for monitoring leukocyte migration. Endothelial cells have been activated with TNF-alpha to initiate the inflammation response. Leukocyte rolling, adhering and migration across the endothelium can be observed in real-time.
Real-time Single Cell Migration Tracking
High magnification view of a single leukocyte migrating across the engineered barrier separating an endothelial lined channel (left side) from a chemo-attractant laced chamber (right side). Note the extreme shape changes that the leukocyte goes through during the migration process.
Real-time Leukocyte Rolling-Adhesion-Migration Assay
A zoomed in view of SynVivo microfluidic chip showing leukocytes interacting with endothelial cells in a microvasculature sized channel adjacent to a chemo-attractant perfused chamber (left side of image). Separating the endothelium channel and the chamber is an engineered porous barrier for monitoring leukocyte migration. Endothelial cells have been activated with TNF-alpha to initiate the inflammation response. Leukocyte rolling, adhering and migration across the endothelium can be observed in real-time.
