Alkaline Phosphatase (AP)

Phosphatases are a class of enzymes that play a key role in the dephosphorylation of organic compounds. Using water as a reactant, phosphatase enzymes catalyze the hydrolysis of various monophosphate esters into phosphoric acid and alcohol. These enzymes are present in both prokaryotes and eukaryotes alike, and are typically classified as either acid phosphatase or alkaline phosphatase. Acidic phosphatases function optimally in acidic pH environments, whereas alkaline phosphatases are optimally active at alkaline pH environments. Together with kinases (enzymes that catalyze the addition of phosphate groups onto molecules), phosphatase enzymes direct a form a post-translational modification that is essential for normal cell biology and pathogenesis.
Alkaline Phospatase
Alkaline phosphatase (ALP) is an 86 kDa homodimeric protein enzyme. It contains two zinc atoms which are crucial to its catalytic function per monomer, and is optimally active at alkaline pH environments. ALP has become a highly sensitive reporter enzyme in immunoassays such as ELISA, as well as, in immunohistochemical (IHC), Northern, Southern and Western blot applications. For these applications, ALP is usually conjugated to a specific primary or secondary antibody and its activity is detected with a signal generating substrate.
AAT Bioquest provides several “mix and read” phosphatase assay kits that are compatible with HTS liquid handling instruments, as well as chromogenic and fluorogenic phosphatase substrate to quantify alkaline phosphatase activity in solutions, cell extracts, or on solid surfaces, such as PVDF membranes.

Product Ordering Information

Table 1. Ordering Info for AP ELISA Assays Products

Cat#Product NameUnit Size
11950Amplite™ Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*500 Tests
11952Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence*500 Tests
11953Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*500 Tests
11954Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Near Infrared Fluorescence*500 Tests
11956Amplite™ Luminometric Alkaline Phosphatase Assay Kit *Luminescence*100 Tests

Table 2. Ordering Info for AP Substrates Products

Cat#Product NameUnit Size
11600FDP [Fluorescein diphosphate, tetraammonium salt] *CAS 217305-49-2*5 mg
11610MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*25 mg
11612MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*10 g
11619pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 4264-83-9*25 mg
11627DiFMUP5 mg
11629SunRed™ Phosphate5 mg
11630PhosLite™ Green1 mg
12512D-Luciferin phosphate *CAS 145613-12-3*1 mg

Table 3. Ordering Info for AP conjugate & Other Reagents Products

Cat#Product NameUnit Size
11622Signal Guard™ phosphatase reaction stopping solution100 mL
16921AP-streptavidin conjugate [Streptavidin-alkaline phosphatase conjugate]1 mg

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