Alkaline Phosphatase (AP)

Phosphatases are a class of enzymes that play a key role in the dephosphorylation of organic compounds. Using water as a reactant, phosphatase enzymes catalyze the hydrolysis of various monophosphate esters into phosphoric acid and alcohol. These enzymes are present in both prokaryotes and eukaryotes alike, and are typically classified as either acid phosphatase or alkaline phosphatase. Acidic phosphatases function optimally in acidic pH environments, whereas alkaline phosphatases are optimally active at alkaline pH environments. Together with kinases (enzymes that catalyze the addition of phosphate groups onto molecules), phosphatase enzymes direct a form a post-translational modification that is essential for normal cell biology and pathogenesis.
Alkaline Phospatase
Alkaline phosphatase (ALP) is an 86 kDa homodimeric protein enzyme. It contains two zinc atoms which are crucial to its catalytic function per monomer, and is optimally active at alkaline pH environments. ALP has become a highly sensitive reporter enzyme in immunoassays such as ELISA, as well as, in immunohistochemical (IHC), Northern, Southern and Western blot applications. For these applications, ALP is usually conjugated to a specific primary or secondary antibody and its activity is detected with a signal generating substrate.
AAT Bioquest provides several “mix and read” phosphatase assay kits that are compatible with HTS liquid handling instruments, as well as chromogenic and fluorogenic phosphatase substrate to quantify alkaline phosphatase activity in solutions, cell extracts, or on solid surfaces, such as PVDF membranes.

Product Ordering Information

Table 1. Ordering Info for AP ELISA Assays Products

Cat# Product Name Unit Size
11950 Amplite™ Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color* 500 Tests
11952 Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence* 500 Tests
11953 Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence* 500 Tests
11954 Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Near Infrared Fluorescence* 500 Tests
11956 Amplite™ Luminometric Alkaline Phosphatase Assay Kit *Luminescence* 100 Tests

Table 2. Ordering Info for AP Substrates Products

Cat# Product Name Unit Size
11600 FDP [Fluorescein diphosphate, tetraammonium salt] *CAS 217305-49-2* 5 mg
11610 MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2* 25 mg
11612 MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2* 10 g
11619 pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 4264-83-9* 25 mg
11627 DiFMUP 5 mg
11629 SunRed™ Phosphate 5 mg
11630 PhosLite™ Green 1 mg
12512 D-Luciferin phosphate *CAS 145613-12-3* 1 mg

Table 3. Ordering Info for AP conjugate & Other Reagents Products

Cat# Product Name Unit Size
11622 Signal Guard™ phosphatase reaction stopping solution 100 mL
16921 AP-streptavidin conjugate [Streptavidin-alkaline phosphatase conjugate] 1 mg

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