Peptides and Oligonucleotides
Peptides are short chain amino acids that perform numerous functions as chemical messengers, hormones, cellular mediators, highly specific stimulators and inhibitors, as well as novel therapeutic treatments for various diseases including Alzheimer’s and cancer. Due to the emerging solid-phase peptide synthesis technologies, synthetic peptides have now been widely used in biochemistry, molecular biology, and pharmaceutical areas. Dye-labeled peptides are powerful tools to investigate cellular structure, analyze receptor-ligand or protein-protein interactions, study cellular transport, and measure enzymatic activity via FRET analysis.
Fluorescent Labeling Dyes
A fluorescent dye can be attached to a peptide/an oligonucleotide at a specific point through a covalent bond depending on the sequence of peptide/oligonucleotide. The linkage between dye and peptide/oligonucleotide is a covalent bond, which is stable and not destructive under most biological conditions. In some cases, a functional linker is introduced between dye and peptide/oligonucleotide to minimize the alteration of peptide/oligonucleotide biological activity. For all the peptide/oligonucleotide labelings, the dye need be attached at a defined position. In general, the preferred fluorescent labels should have high fluorescence quantum yields and retain the biological activities of the unlabeled biomolecules. AAT Bioquest offers a variety of fluorescent labeling dyes for facilitating the conjugation of dyes to peptide/oligonucleotide that are used for biological studies. These fluorescent dyes include coumarins, fluoresceins, rhodamines and cyanines. Among them, our Tide Fluor™, California Red™, SunRed™ and Helix Fluor™ dyes are optimized for labeling peptides and oligonucleotides.
Cyanine Dye Family and Other Classic Dyes