The much-anticipated Abbkine products coming! To provide the excellent products, Abbkine control the quality strictly. Finally, three new shining stars on display.

First one: SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8)

SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (Formazan), which is soluble in the tissue culture medium. The amount of the formazan in cells is directly proportional to the number of living cells. The reagent has the characteristics of high sensitivity, good stability and convenient operation.

Advantages

1, High sensitivity: More accurate detection results;

2, Good stability: Can be stored at -20℃ for more than 2 years;

3, High safety: Less cytotoxicity, almost no impact on subsequent experiments.

Sensitivity data display

Fig.1 Comparison between SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) (Cat # : BMU106-EN) and three international brands A, B and C in the detection of HEK293 cells with different number of living cells was performed. The results showed that the SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) had the best sensitivity.

Stability data display

Fig.2 The  SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8)(BMU106-EN) and the International brand A kit were treated at 37℃ for 7 days and compared in different quantities of HEK293 cell activity detection. The results showed that the SuperKine™ Hypersensitive Cell Proliferation Assay kit (CCK-8) was more stable.

The purpose of the CCK-8

• Cell proliferation assay: Cell proliferation assay is used in many fields, such as tumor research, post-injury repair, etc.
• Cytotoxicity measurement: Study the effects of certain stimuli on cell activity and proliferation, such as the effects of UV, low oxygen or other chemicals, harmful drugs, etc.
• Drug screening: CCK-8 is often used to determine the IC50 value of a drug, or to determine the toxicity and safe and effective concentration of a drug.
• Tumor drug sensitivity test: Draw drug concentration-tumor cell growth inhibition curve with THE help of CCK8 kit, calculate IC50, IC90, sensitivity index and area under the inhibition curve, and evaluate drug effect.
• Experiments on cell toxicity or proliferation of microorganisms

Second one ：ExKine™ Pro Total Protein Extraction Kit for Plant Tissues

This product is a new generation of ultra-fast protein extraction tools. More and more evidence suggests that the most commonly used RIPA buffer may lead to random loss of proteins, resulting in a lot of difficult data to interpret. ExKine™ Pro Plant Tissue Total Protein Extraction Kit (Column method) uses centrifugal string extraction technology combined with optimized lysis buffer to extract total protein faster and more efficiently without liquid nitrogen grinding, enabling more accurate WB results.

Advantages:

Quick: finish all steps in 8 minutes.

Complete: applicable to complete samples – plant tissue leaves, seeds, soft stems, roots.

High protein yield, from 50-200 mg plant tissue, total protein yield can reach 2-8 mg/mL.

Save : No liquid nitrogen grinding, optimized cracking buffer for more efficient protein extraction.

Widely: The obtained protein can be used for SDS-PAGE and WB.

Fig.3 SDS-PAGA diagram of plant protein map (using Abbkine KTP3008) : Lane 1- soybean, lane 2- corn, lane 3- spinach, lane 4- Arabidopsis.

Third one: SDS-PAGE Protein Sample Loading Buffer (5X)

This product is a modified protein loading buffer with bromophenol blue dye and 5 times concentration. Sds-page protein loading buffer (5×) uses a more stable, more effective and odor-free reducing agent instead of conventional DTT or 2-ME for the loading of conventional reducing SDS-PAGE protein samples.

Advantages:

• Ready-to-use type;
• A reducing agent with better stability, better reduction effect and no unpleasant smell is used;
• Replace traditional DTT or 2-ME.

Fig.4 ExKine™ Pro Total Protein Extraction Kit for Animal Cultured Cells/Tissues（Cat：KTP3007）SDS-page of the extracted protein after adding protein loading buffer (5X) (KTD3003); The left swimming lane is the effect picture of Abbkine product, and the rest of the swimming lane is the effect picture of other brands.