Western blotting is a hybridization technique that combines high-resolution gel electrophoresis and immunochemical analysis techniques. Western blotting has the advantages of large analysis capacity, high sensitivity, and strong specificity. It is one of the most commonly used methods for detecting protein characteristics, expression and distribution, such as qualitative and quantitative detection of tissue antigens, quality determination of polypeptide molecules, and antibody or antigen detection of virus, etc. The immunoblotting test (IBT) is also known as enzyme linked immunoelectrotransfer blot (EITB). Because it is similar to the Southern blot method for detecting nucleic acid established by Southern earlier, it is also called Western- blot.
Abbkine’s WB product portfolio includes: protein extraction series, protein purification series, protease inhibitor set cocktail, primary antibody, HRP secondary antibody, ECL color reagent, protein marker, IFkine secondary antibody, DyLight secondary antibody, etc. The results of the experiment can be used not only for the qualitative and quantitative target protein, but also for the subsequent analysis of protein-protein, protein-DNA, and protein-RNA interactions.