Previously, we provided detailed knowledge about fatty acid metabolism, including fatty acid anabolism and catabolism, and also introduced two biochemical indicators related to fatty acid metabolism, including:
1. Fatty acid synthase
Fatty acid synthase (FAS) is a multifunctional homodimeric enzyme protein that is the primary enzyme required for the anabolic conversion of dietary carbohydrates to fatty acids. Fatty acid synthase catalyzes the conversion of acetyl coenzyme A and malonyl coenzyme A to long-chain saturated fatty acids in the presence of NADPH. Human fatty acid synthase is a large homodimeric multifunctional enzyme for the synthesis of palmitic acid. The unique carboxy-terminal thioesterase structural domain of fatty acid synthase hydrolyzes the growing fatty acid chains and plays a key role in regulating the length of the released fatty acid chains. In addition, the upregulation of human fatty acid synthase in a variety of cancers makes thioesterase a candidate target for therapeutic treatment. Fatty acid synthases of animal tissues are complex multifunctional enzymes composed of two identical monomers.
Significance of determination of FAS:
FAS is a key enzyme in fatty acid synthesis, which catalyzes acetyl-CoA and malonyl-CoA to produce long-chain fatty acids. FAS is widely expressed in various tissues and cells, and is abundantly expressed in mammalian liver, kidney, brain, lung, mammary gland and adipose tissue.
Principle of FAS determination：
The principle is that FAS catalyzes the formation of long-chain fatty acids from acetyl CoA, malonyl CoA and NADPH, and NADP+;NADPH has an absorption peak at 340 nm, but NADP+ does not, and the FAS activity is calculated by measuring the light absorption decline rate of 340 nm.