Affinity Ligand Coated Microspheres

Our streptavidin and SuperAvidin™ coated microspheres offer simple and extremely stable binding of biotinylated molecules, such as antibodies, peptides, and oligonucleotides for use in diagnostics, screening, and affinity purifications. See TechNote 101, Affinity Ligand coated microspheres. Streptavidin coated microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 2mL, 5mL, or 10mL. See also our BioMag^® magnetic particle offerings.

REFERENCES

• Nandin IS, Fong C, Deantonio C, Torreno-Pina J, Pecetta S, Maldonado P, … Batista, F.(2017) Novel in vitro booster vaccination to rapidly generate antigen-specific human monoclonal antibodies. J Exp Med.20170633. (0.11µm Streptavidin-coated microspheres)
• Wu SJ,Chan A, Kado CI. (2004) Detection of PCR amplicons from bacterial pathogens using microsphere agglutination J Microbiol Methods Vol 56, Issue 3, 395-400 (5.7µm Streptavidin-coated microspheres)
• Walter S, HerrgenL, Schoor O, Jung G, Wernet D, Bühring HJ, … Stevanović S.(2003) Cutting Edge: Predetermined Avidity of Human CD8 T Cells Expanded on Calibrated MHC/Anti-CD28-Coated Microspheres. J Immunol. 171(10)4974-4978 (5.6µm Streptavidin as artificial-antigen presenting cells [aAPC])
• Jeffers, Faye, et al. “Mucin–lectin interactions assessed by flow cytometry.” Carbohydrate research 345.10 (2010): 1486-1491.(3.09µm Streptavidin PS & 2.01µm Silica)

The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres (UMC catalog codes) are highly uniform and spherical. Fluorescent magnetic microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL. See spectra for available fluorescent dyes. See also our BioMag^® magnetic particle offerings.

REFERENCES

• Pitsillides, CM, Joe, EK, Wei, X, Anderson, RR, & Lin, CP. (2003). Selective cell targeting with light-absorbing microparticles and nanoparticles. Biophysical journal, 84(6), 4023-4032.(0.83µm Streptavidin magnetic)

References

• Linz TH, Henley WH, & Ramsey JM. (2017) Photobleaching kinetics-based bead encoding for multiplexed bioassays. Lab on a Chip, 17(6), 1076-1082. (3µm ProMag Streptavidin with immobilized b-PE and b-Qdot labels)

REFERENCES

• Chang D, Tram K, Li B, Feng Q, Shen Z, Lee CH, … & Li, Y. (2017). Detection of DNA Amplicons of Polymerase Chain Reaction Using Litmus Test. Scientific reports, 7(1), 3110.(Cat. BM551BioMag Streptavidin used in colorimetric detection of C. difficile)

REFERENCES

• Bhattacharyya, K., Goldschmidt, B. S., & Viator, J. A. (2016). Detection and capture of breast cancer cells with photoacoustic flow cytometry. Journal of biomedical optics, 21(8), 087007. (320nm streptavidin-coated Flash Red fluorescent nanoparticles + biotin-EpCAM)

Streptavidin-coated dyed microspheres supplied as ~1% solids (w/v) aqueous suspensions.

References

• Wang Y, Yan W, Fu S, Hu S, Wang Y, Xu J, & Ye C. (2018). Multiple cross displacement amplification coupled with nanoparticles-based lateral flow biosensor for detection of Staphylococcus aureus and identification of methicillin-resistant S. aureus. Frontiers in Microbiology, 9.(0.129µm Streptavidin-coated Crimson Red nanoparticles)

SiO2 with a streptavidin-coated surface for the attachment of biomolecules (See also PDS 721.pdf). Silica streptavidin microspheres are sold in these standard amounts: 1mL, 2mL, 5mL, and 10mL. Available at 1% solids (w/v), in three nominal mean diameters: ~0.5µm, ~1.0µm, and ~5.0µm.

REFERENCES

• Blumberg, S, Gajraj, A, Pennington, M, Meiners, J (2005) Three-Dimensional Characterization of Tethered Microspheres by Total Internal Reflection Fluorescence Microscopy. Biophysical Journal V89(2)p1272–1281
• Wang J, Xu D, Kawde A, Polsky R (2001) Metal Nanoparticle-Based Electrochemical Stripping Potentiometric Detection of DNA Hybridization Anal Chem. 73(22), 5576-5581

Our biotin-coated microspheres have been fully characterized in terms of their ability to bind free avidin, and therefore will require minimal optimization when determining the correct concentration of ligand to be bound. Biotin coated beads are supplied as ~1% solids (w/v) aqueous suspensions. See TechNote 101, Affinity Ligand coated microspheres, for sample applications and procedures. See also our BioMag^® offerings.

Protein A and Protein G are proteins that readily bind the Fc regions of many types of antibodies. (See TSD 0025 ) For this reason, they are used extensively for affinity purification of antibodies from serum or cell culture supernatant, and for capture of immunoprecipitated protein complexes. When Protein A and Protein G microspheres are used as reagents in tests and assays, the immobilized antibodies may be crosslinked to the Protein A- or Protein G-coated support for long-term stability. See TechNote 101, Affinity Ligand coated microspheres. Protein A and Protein G coated microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 2mL, 5mL, or 10mL. See also our BioMag^® offerings.

REFERENCES

• Reynolds NM, Mohammadalipour A, Hall CR, Adib AA, Farnoud AM, Burdick MM. (2018) Galectin-1 Influences Breast Cancer Cell Adhesion to E-selectin Via Ligand Intermediaries. Cell Mol Bioeng; 11(1):37-52. Note:  (Protein A / E-selectin [hFc] beads used to investigate E-selectin / Gal-1 mediated adhesion in breast cancer monolayers)

BioMag^® secondary antibody particles are used extensively in the capture of immune complexes for immunoprecipitation and antibody-labeled cells for indirect cell separation. Secondary antibody coatings also afford a convenient method for binding specific primary antibodies to BioMag^® particles.

Our ConA- and WGA coated BioMag^®Plus microparticles provide a convenient means for isolating glycoproteins from serum or cell lysate, and for investigating other lectin/glycan-mediated processes. The BioMag^®Plus magnetic particle format provides high surface area, and permits easy and efficient separations.