Determination of protein kinetics using switchSENSE®
The switchSENSE® technology of Dynamic Biosensors utilizes a novel electro-switchable biosurface to provide researchers and commercial laboratories the ability to characterize interactions between molecules in real-time. This technology is unlike existing methodologies in that it combines high sensitivity kinetics with structural information on size, shape and conformation providing a new depth and understanding of the interaction.
Studies are performed on a re-usable biochip, generated using familiar coupling and hybridization methods. Within this biochip, DNA levers are embedded onto a series of gold electrodes. These nanolevers serve either as target for molecular interactions themselves, or hold other interaction partners. To characterize interactions, the DRX instrument is used to bring about deliberate movement of these nanolevers by altering the voltage across the gold surface. When interactions occur, these movements are affected and in turn, used in the calculation of kinetic and biophysical information.