Winner of CONNECT’s Most Innovative Product Award for Life Science Diagnostics & Research Tools
StemoniX® microHeart® platform provides a structured environment for human induced pluripotent stem cell (iPSC) derived cardiac cells to passively align into a physiologically relevant structure to closely represent human heart tissue. Cardiac cells on a structured microHeart plate resemble heart tissue alignment while standard flat cultureware results in a random arrangement. microHeart Cell Ready and Assay Ready products provide an ideal model for cardiotoxicity and cardiac safety studies, high throughput screening and drug discovery.
 |
 |
 |
Cardiomyocytes plated on microHeart align and organize with the corresponding plate substrate topography compared to lack of cell organization in standard flat cultureware. microHeart’s structured substrate enables accelerated features of cell maturity when compared to standard cultureware with a flat substrate. The enhanced cell maturity results in functionality and responses which can improve assay outcomes and reduce assay cost. The effectiveness of the structured substrate is evident by:
|
 |
microHeart cardiomyocytes contract mostly along one single direction, more closely resembling the physiological contractile behavior of human heart tissue. Arrows highlight the direction of contraction measured by motion tracking analysis. Contraction vector maps confirm unidirectionality of contraction in microHeart compared to randomly directed contraction in standard flat cultureware.
|
Standard Flat Cultureware  |
StemoniX microHeart  |
Optical flow analysis script obtained from Huebsch et. al. , Tissue Eng Part C Methods (2015) Contraction vector maps obtained with Sony Biotech SI8000 Motion Vector Software
Cardiomyocyte cells plated on microHeart displayed readily identifiable and aligned sarcomeres compared to an immature pattern in standard flat cultureware.
| Standard Flat Cultureware
DNA – cTNT – Sarcomeric αActin |
StemoniX microHeart
DNA – cTNT – Sarcomeric αActin |
Representative immunostaining of the cardiac cell junction marker plakoglobin (PG) in cardiomyocytes plated on standard cultureware or microHeart. Arrows highlight distal intercalated discs. On microHeart, PG is more frequently restricted to distal intercalated discs, as opposed to being distributed throughout the cell perimeter as in standard cell cultureware.
|
Standard Flat Cultureware  DNA – Plakaglobin |
StemoniX microHeart  DNA – Plakaglobin |
|
High throughput calcium flux analysis indicates faster and more efficient calcium handling of human cardiomycoytes in microHeart. 
Significance in unpaired t-test, **** p<0.0001 Measurements made with a Molecular Devices FLIPR Tetra® High-Throughput Cellular Screening System |
Cardiomyocytes plated on microHeart express increased levels of key cardiac physiology genes compared to standard flat cultureware.
|
microHeart Assay Ready plate products are pre-plated with StemoniX human iPSC derived cardiac cells providing structured cell organization and accelerated features of maturity, delivered in ambient conditions and ready for assay use to simplify your workflow.
microHeart Cell Ready plate products are ready for plating with your cells allowing for structured cell organization and accelerated features of maturity.
Safety Pharmacology & Investigative Toxicology
|
Drug Discovery & Screening
|
Disease Modeling
|
Platform Compatibility
|
| Product Name | Catalog # | |
|---|---|---|
| microHeart Assay Ready 96-well Plate | MHARX-AA-0096 | |
| microHeart Assay Ready 384-well Plate | MHARX-AA-0384 | |
| microHeart Assay Ready 1536-well Plate | Call for Information | |
| microHeart Cell Ready 96-well Plate | MHCRX-AA-0096 | |
| microHeart Cell Ready 384-well Plate | MHCRX-AA-0384 | |
| microHeart Cell Ready 1536-well Plate | Call for Information | |
outstanding technical support
we offer a full product guarantee
we offer free delivery to UK universities and non profit organisations
