Creative Enzymes uses its expertise in enzyme manufacturing to supply high quality, cost-effective enzymes, coenzymes, zymogens, substrates and inhibitors to academic, commercial, and government laboratories worldwide.

Creative Enzymes’ products are used for a diverse range of applications, from metabolism, immunology, cancer and infectious disease research to drug discovery, screening, development and manufacture.


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Researchers use these enzymes to precisely manipulate and modify cellular activities to further understand processes such as neurotransmission (such as native forms of Human Butyrylcholinesterase, Human Acetylcholinesterase, and Human Butyrylcholinesterase), calcium signalling (including native Porcine Adenosine 5′-Triphosphatase), cell stress and protein re-folding (such as Protein Disulfide Isomerase (PDI), Superoxide Dismutase, Thioredoxin), signal transduction (kinases and phosphatases), apoptosis (Cathepsins, Granzyme A, Granzyme B) and other processes. For antibody production, Creative Enzymes offers a full range of high quality IgG proteases and IgG glycosidase enzymes to digest IgG to generate Fab and Fc fragments for subsequent analysis or modification purposes.  The high purity of Creative Enzymes’ products make them suitable for use in diagnostic and research assays such as ELISA. Enzymes include oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases.

Creative Enzymes offers a variety of custom services indlucing the following:

  • Enzyme Discovery
  • Enzymology Assays
  • Screening of Substrates, Inhibitors, and Other Ligands
  • Enzyme Engineering and Modification
  • Enzyme Conjugation
  • Biocatalysis Services
  • Enzyme Expression and Purification
  • Enzyme Stabilization and Formulation
  • Industrial Enzyme Production

Find out more below:

Creative Enzymes holds its unique position in the enzyme market to provide strategic solutions for finding promising industrial enzymes from various natural sources, such as soil microbes, plants and animals. We combine different advanced techniques, including bioinformatics tools, high-throughput techniques, separation and isolation, and statistical designed experiments, to provide a full service of enzyme discovery from initial sample preparation to final identification of novel enzymes.

Enzyme discovery with bioinformatic tools and HT techniquesFigure 1. Main Strategies for Novel Enzymes Discovery
(Bioprocess and Biosystems Engineering 2017)

Enzymes have found applications as biocatalysts with high efficiency and selectivity in manifold fields of chemistry and biology. Yet, only a small portion of natural enzymes have been exploited industrially. Traditional strategies to isolate novel enzymes from wild-type strains, including DNA extraction, heterologous protein expression and a generic assay, are usually time-consuming and with limited sensitivity. Creative Enzymes has built professional high-throughput platforms for enzyme discovery with significant advantages:

  • Advanced bioinformatic tools and genome mining approaches
  • Metagenomic libraries construction
  • High throughput screening, analysis and characterization
  • Statistical design of experiments
  • Seamless separation and purification
  • Enzyme de novo identification

Strategies for enzyme discovery from nature can be categorized into two main methodologies, which could be combined for best results. The first involves direct detection of the desired enzymatic activity. Based on next-generation sequencing (NGS) methods, Creative Enzymes constructs metagenomic libraries and develops high-throughput screening for enzymatic activity. The second strategy involves function prediction and in silico screening. Creative Enzymes applies genome mining approaches for enzyme discovery based on similarities of their DNA sequences or 3D structures to those of known activity. We also investigate interactions among different enzymes in case a series of enzymes need to stay in the system for the best performance.

Creative Enzymes conducts structure and activity characterization for identification of novel enzymes. We offer services on demand, including primary structure determination, disulfide bond analysis, and 3D structure determination.

Enzyme discovery represents a growing field with many possible applications including animal feed, biocatalysts, pharmaceuticals and chemical processing. Creative Enzymes has tight connections in both academia and industry, and thus has accumulated rich experience to provide the most advanced solutions for our customers.

Our Highlighted Platforms Include

1. Screening for enzymes from natural sources.

1.1 Enzyme discovery by direct activity measurement
1.2 Metagenomics and exploration of non-culturable

2. Function prediction and screening.

2.1 Sequence-based screening
2.2 3D structure-guided screening
2.3 Protein interactions

3. Structure characterization

3.1 Primary structure determination
3.2 Disulfide bond analysis
3.3 3D structure determination

Enzyme Discovery Workflow

Enzyme discovery workflow

Creative Enzymes is a renowned service provider, supporting a majority of the enzyme assay market. Creative Enzymes endeavors to perfect all types of enzymology assays. We are able to perform individual tests at the most precise level, as well as design and complete a whole package of services as the solution to a thematic project.

  • Specialized analysis and assessment
  • Full range of assay equipment and platforms
  • High-throughput starting from 200 candidates per assay
  • Sensitive miniaturized assays
  • Rapid and accurate results
  • One-stop solution

Enzymology Assays Workflow

Enzymology assays workflow

Creative Enzymes is happy to help with the challenges in enzymology assays. Based on the resources that are required for the task, we list the enzymology assays below in the order of increasing complexity:

Services Features Price
Enzymology assays Activity measurement
  • kinases, phosphatases,proteinases,deacetylase,peptidase, esterase, and other enzymes
  • Kit assays and customized assays
  • High-throughput screening
Get a quote
Development of analysis methods
  • New assay development for unprecedented condition and newly discovered enzyme
  • Challenging assay such as reversible reactions and coupled tests
Enzyme kinetics
  • Determine virtually all parameters for an enzyme such as  Vmax,  Kd, KM kcatk1, and k-1
Enzyme catalytic mechanisms
  • Multiple technologies including crystallography (structural biology), site-directed mutagenesis, reporter group labeling of the substrate or inhibitor, kinetic measurement with the isotope effect etc

FAQ:
Q1: Can I use an “existing” protocol for my enzyme assay?

It is to be noted that all enzymes reach their optimum activity under different conditions, whereas in a high-throughput screening, one single reaction condition is often used. As a result, the differences in the activity levels of enzymes may be true only in the current condition and cannot be used for quantitative comparison. Another challenge that frequently troubles researchers is that detection of formation of the product or the consumption of the substrate could be strongly disturbed by other ingredients in the reaction matrix, such as inactive solutes or other enzymes. In such cases, the signal to noise ratio must be considered for accurate measurement. Proper selection of the detection method and parameters often gives solutions to such problems.

Creative Enzymes has been a pioneer in the enzymology assay area. We served customers to optimize a product or to set a solid foundation for further research. You can find any level and scale of enzymology assays available at Creative Enzymes.

Creative Enzymes is a leading professional in the field of enzyme ligand screening. We are specialized in rapid and accurate evaluation of substrates, inhibitors, and other ligands for specific enzymes, based on high-throughput experiments or computational simulation results. In other cases, we also provide screening of active enzymes against specific substrates or inhibitors, which would help to identify the functioning enzymes in a pathway or the biological target of a small molecule.

Features

  • Advanced: state-of-the-art candidates handling and tracking
  • Huge screening platform: 240,000 compounds, with 3 library subsets designed for specific screening needs
  • Updated: each candidate is up-to-date, less than 2 years old at time of selection
  • Reliable: candidates have been rigorously selected to exclude undesirable features
  • Development-ready: all candidates or building blocks are available in trial sizes

Screening Services Workflow

Screening Services Workflow by Creative Enzymes

# Required for the guaranteed packages but optional for Custom services.

Screening Services Selection Guide

Services Highlighted Services Features Price
Substrate screening and identification
  • Best substrate for an enzymatic reaction
  • Natural substrates of an enzyme
Give the key information of the natural function of the enzyme. Get a quote
Inhibitor screening and design
  • Virtual screening of enzyme inhibitors
  • High-throughput screening of inhibitors
  • Structure-based inhibitor design
  • Ligand-based inhibitor design
Helpful in drug discovery, fundamental research and other industrial applications. Get a quote
Target enzyme identification
  • Enzyme screening against substrates
  • Identification of a target enzyme
Helpful in medical, diagnostic metabolomics. Get a quote
Identification and screening of other ligands
  • Screening of enzyme activators
  • Evaluation of cofactors and additives
  • Detection of enzyme aggregation and oligomerization
  • Enzyme folding facilitators
-Helpful for enzyme stabilization, storage, and optimization of reaction conditions.
-Helpful in correcting enzyme folding and enzyme stability.
Get a quote

For all these different services, we provide fully customizable screening services in consultation with clients.  To discuss project details, please contact us

Supported by outstanding enzymology researchers, Creative Enzymes is able to design and perform various screening tests for a variety of applications. Once your request is received, you can rely on Creative Enzymes to bring the package of solution and deliver the accurate results.

Creative Enzymes provides the enzyme engineering and modification service to help clients with the increasing needs in improving enzyme activity and properties and creating the perfect biocatalyst for a new reaction. Creative Enzymes is one of the few companies that provide solutions to such challenging tasks. After many years in serving companies and research institutes, we have demonstrated high reliability of our services.

  • Specialized expertise and resources
  • Powerful computational modeling
  • Construction of large mutant libraries
  • High throughput screening
  • Results analysis

Enzymes engineering and modification workflow

Wokflow of enzyme engineering and modificatoin

Creative Enzymes has strong ability to offer one-stop service for enzyme engineering and modification. Our services are listed below:

  Services Price
Enzyme Engineering Directed evolution Get a quote
Rational design
De novo enzyme engineering
Site-directed mutagenesis
Random mutagenesis and DNA shuffling
Phage display and mRNA display
Incorporation of unnatural amino acids
Enzyme Modification Immobilization Get a quote
Encapsulation
Covalent modifications (such as PEGylation)
Enzyme labelling
Synthetic Enzymes Synzymes Get a quote
Abzymes

FAQ:
Q1: Why should I modify my enzyme?

The existing enzymes can go through certain structural alterations and hence, the resulted function and catalytic activity allow new catalyzed pathways of reactions. In addition, modification could also enhance biochemical properties, pharmacokinetics, and stability. Enzymes can be physically modified in immobilization and encapsulation. Researchers also extent naturally occurring chemical reactions of enzymes to other chemicals such as polymers, aldehydes, imidoesters and anhydrides, to give enzymes more divers properties. In addition, chemists have developed a large set of reactions that label the enzymes through post-expression modifications. Many properties of an enzyme may be modified to achieve the objective:

Enzyme Engineering and Modification-(2)

Engineered enzymes have already showed growing use in therapeutic and industrial products. Creative Enzymes is glad to help every customer in the search of improved and optimized enzymes. Our strong technical advantage is based on a large community of biochemical scientists, who bring the latest progresses in enzymology to serve your need. You will stay with the most recent enzyme engineering technics when you choose to partner with Creative Enzymes.

Creative Enzymes’s decades of experience on enzyme conjugation enables enzyme conjugates with various functional molecules, such as proteins/peptides, antibodies, polymers including polysaccharide, small molecules, and nucleic acids. With advanced technologies and instruments, we develop and prepare enzyme conjugates not only by innovative conjugation reactions, but also through improvement in protein expression, isolation and purification, and validation and characterization. We provide conjugation services for enzyme production based on customer requirements for various studies and applications, including immunosorbent assays (ELISAs), western blotting, immunostaining, and biosensors.

Enzyme conjugates with different moelcules.Figure 1 Protein conjugation with other molecules.
(Chemical Reviews 2018)

Enzymes are used as signal generating reporters when conjugating to other molecules. Various reporter enzymes, such as horseradish peroxidase (HRP), Alkaline phosphatase (AP) and many others, can be attached to antibodies, proteins and nucleic acid for applications in immunoassays and biosensors. Also, enzymes can be modified with conjugation for desired properties and functions, especially stability. In pharmaceutical applications, these conjugates can be used as smart “vehicles” for drug delivery. To meet various requirements from customers, Creative Enzymes has built professional and updated enzyme conjugation platforms with plenty of advantages:

  • Nearly 1:1 ratio of enzyme to interested target
  • Optimized sample preparation and conjugation
  • High product yield and purity
  • Strict validation and characterization
  • Scale-up production up to kilograms

Enzymes can be conjugated with other molecules via chemical, enzymatic, or chemoenzymatic formation of new covalent bonds. Usually, small linkers such as glutaraldehyde or protein tags are used in these conjugations. Those enzyme conjugates to one or multiple types of molecules based on applications in different fields. For immunoassays and other related applications, Creative Enzymes provides “activated” enzymes in purified, activated, and kit formats for easy conjugation. For specific applications in pharmaceutical/non-pharmaceutical industry, Creative Enzymes offers desired enzyme conjugates with developed purification and scale-up procedures. Creative Enzymes also has the ability to conduct characterization and validation of target conjugates product.

Enzyme conjugation represents a booming market in medical diagnostics, gene expression analysis, detection of infectious diseases and biological warfare agents among others. Creative Enzymes has tight connections in both academia and industry, and thus has accumulated much experience to provide the latest solutions for our customers.

Enzyme Discovery Workflow

Enzyme Conjugation

Our Highlighted Platforms Include

1. Enzyme conjugation with small molecules.
2. Enzyme conjugation with protein/peptides.
3. Enzyme conjugation with polymers.
4. Enzyme conjugation with antibodies.
5. Enzyme conjugation with nucleic acids.

Professional Biocatalysis Services by Creative Enzymes

Creative Enzymes has long demonstrated expertise and reliability in development and establishment of biocatalysis systems. We provide products, services, and consultation covering every step in the whole process of a biocatalyst development, including the design, modification, expression, purification, production, and validation of specific enzymatic or microbial systems that catalyze the desired reactions.

The natural, green biological catalysts attract fast growing attentions for many benefits, such as sustainability, high efficiency, and little waste footprint. However, developing and setting up such systems could be challenging, given that the nature of these biocatalysts is distinct from traditional ones. Furthermore, maintaining a biocatalytic mechanism performing as a stable and consistent manner also requires engineering design and development on the whole system, in addition to the molecular biology and biochemistry techniques that are essential to start feasibility evaluation on the biological catalysts. Creative Enzymes presents the professional development platform for biocatalysts by integrating the expertise on molecular biology and protein engineering, and experience on development of industrial processes. Creative Enzymes has extensive partnership with academic institutes working at the cutting edge technologies of biocatalysis. This enables Creative Enzymes to provide the latest solutions and unbiased selection of the best products for our clients.

Features

  • Biodegradable and environmental friendly reagents and solvents
  • Services and consultation tailored to specific development needs
  • Efficient synthetic and processing routes
  • Decreased cost and turnaround time through minimization of side reactions, by-products and impurities
  • Optimization and enhancement of conversion rates, yields, and selectivity
  • Design and setup of self-regenerating or low-maintenance systems

Biocatalysis Services Workflow

Biocatalysis Services Workflow by Creative Enzymes

Our Highlighted Platforms Include

  • Substrate profiling
  • Computational modeling for active sites
  • Selection and modification of additives, cofactors
  • Catalytic assays
  • Mechanism modeling & investigation
  • Natural biocatalyst sampling and screening
  • Codon optimization
  • Rational design
  • Continuous directed evolution
  • Modification and immobilization
  • Multi-enzyme cascade reaction systems
  • Whole cell biocatalysts
  • Metabolic pathway analysis
  • Pathway design and strain optimization
  • Genome engineering
  • Process optimization/redesign
  • Process scale-up

For all these different services, we provide fully innovative and bespoke services and consultation to customers. To discuss project details, please contact us

Creative Enzymes has decades of experiences in enzyme expression and purification. We provide a series of services including gene cloning, expression, and fermentation optimization and production of enzymes. The crude enzyme product is then purified properly to be stored or used with stable activities over time. On the top of enzyme expression, we also provide the purification service for enzymes from natural sources or customized designs.

  • Stable and full-fledged platform
  • Full range of expression systems
  • From 0.5 mg to > 100 kg capacity
  • Simultaneous screening of >16 conditions
  • Delivered > 200 custom enzymes
  • Consultation from cloning to purification


Enzymes Expression Service Workflow

Enzymes expression service workflow

Creative Enzymes will best tailor these services based on customers’ research needs. With extensive experiences in enzyme production, we are able to offer the best services:

For enzyme expression and production

  Host Services Timeline Price
Enzyme Expression & Production Gene identification, synthesis and cloning 2 weeks Get a quote
Codon optimization 1-2 weeks
Selection and modification of expression system 1 week
Selection and construction of expression vector 1-2 weeks
Tag selection 1 week
Protein refolding 1-2 weeks
Post-translational modifications 2-3 weeks
Identification and optimization of enzyme expression conditions 1-2 weeks
Scale-up enzyme expression 1-2 weeks
Fermentation development and consulting 2-3 weeks

For enzyme purification

  Services Features Timeline Price
Enzymes Purification Enzyme purification
  • 6xHis; GST; MBP; FLAG tag purification
  • Purity >85%
1 week Get a quote
Endotoxin removal
  • Estimation of endotoxin removal

efficiency and protein loss rate

  • Endotoxin removal percentage: 90-99%, Protein Recovery range: >85 %
1-2 weeks
Enzyme characterization and quality certification
  • SDS-PAGE; HPLC; WB; Mass Spec
  • Concentration & Activity determination
  • Absorption spectrum; N-terminal sequencing
1-2 weeks


FAQ

Q1: What expression host should I use for my enzyme?

Answer: Using our powerful expression platform, we will select the most suitable host based on the need of specific enzymes, with consideration of purity, biological integrity, and potential toxicity. We provide competent and stable expression systems included bacterial expression systems, yeast expression systems, baculovirus expression systems, and mammalian cell expression systems, which all have unique advantages as listed below.

Enzyme Expression systems in Creative Enzymes

For special enzymes that have not been expressed in a certain system, we also offer expression system screening services.

Creative Enzymes shows complete dedication to all enzyme-related solutions by providing stabilization and formulation services. We offer the whole package of services to address enzyme stability issues ranging from stabilization, stability tests, to formulation development. Our expertise will assure you that the developed products are of high efficacy, quality, and safety, regardless of the purity, identify, or quantity of the starting samples.

Features

  • Cutting edge facilities & innovative techniques.
  • A wide range of enzyme formulations, from liquid and various forms of solid products to immobilized enzymes.
  • Capable of fast development and scale-up of virtually any new formulation and process
  • Formulation solutions for all volumes, grams to the 100 kg scale.
  • A wide array of analytical tools for characterization and troubleshooting

Stabilization and Formulation Services Workflow

Enzyme Formulation Services Workflow by Creative Enzymes

Our Stabilization and Formulation Services Include:

Services Highlighted Services Price
Enzyme stabilization Immobilization, including adsorption, covalent binding, cross-linking, entrapment, and encapsulation Get a quote
Micelle and reversed micelle Get a quote
Chemical modification Get a quote
Enzyme engineering Get a quote
Ionic liquid and polymer coating Get a quote
Use of additives Get a quote
Stabilization in organic solvents Get a quote
Enzyme stability tests Real-time and accelerated test Get a quote
Enzyme pH stability Get a quote
Enzyme thermal stability Get a quote
Ionic strength test on enzyme stability Get a quote
Enzyme formulation Get a quote

For all these services, we provide fully innovative and bespoke consultation with customers. To discuss project details, please call 1-631-562-8517 or e-mail us at: info@creative-enzymes.com, or use our contact page: Contact.

Creative Enzymes has worked closely with the customers to meet their needs in this last step of delivering an enzyme product. With all the expertise and experience in house, we are confident to solve any challenge you may have during commercializing the enzyme product.

FAQ
Q1: Do I need enzyme stabilization & formulation as part of product development?

A: Enzyme formulation is one last and important step in commercializing an enzyme containing product. Nonetheless, the step has not yet received enough notice for its key role in effectively delivering the intended performance of the product. The main purpose of formulation is to preserve the enzyme in a ready-to-use form until consumed by the customer. Many companies spent a significant amount of resource and time on developing and testing the new enzyme product in details to deliver perfect biochemistry. However, final customers are only interested in the safety, stability, and aesthetics of the products on the shelf. Therefore, the product is only completed when it goes into the final package. At Creative Enzymes, we understand the importance of stable enzyme activity and will help you keep the enzymes stable during long-term storage. We also perform proper aging tests that predict the shelf life of an enzyme product using acceleration tests and kinetic models. In the end, we are happy to develop and optimize the product formulation for a specific enzyme.

Creative Enzymes is a leading company of contract manufacturing and development. Our high-quality services are focused on enzyme related projects and serve customer’s need in many industries. We develop and manufacture specialty enzymes in both small and large quantities for various applications, such as food & beverage, chemical processing, life science, and waste management. We provide reliable quality at a competitive price, serving all processes from discovery to commercialization.

Unlike conventional small molecules, enzymes are usually produced in cultures of living cells. Enzyme manufacturing combines various fields of science, such as microbiology, biochemistry, and engineering. With 10 years of experience in enzyme production, we created exclusive and unique enzyme solutions designed to satisfy the unmet need in the marketplace.

BIOTECHNOLOGICAL-PROCESS-OF-ENZYME-PRODUCTION

  • Production efficiency prediction
  • Seamless scaling up from development to manufacturing
  • Capacities from grams to tons
  • Technology transfer with documents and experimental data
  • Complete solutions for large-scale production
Feasibility AssessmentBioinformatics & MetageneticsCell Biology & Strain ImprovementTrial Fermentation & SamplingProduct Analysis & QualificationProcess Development & OptimizationFermentation & Scaling UpPurification & RecoveryFinish & FillProcess of industrial production

Regardless of what challenges you may face during enzyme manufacturing, Creative Enzymes is always ready to provide enough products for the next development stage. With the trust of thousands of industrial customers, we are happy to serve you with the most satisfying services. To get more information of these services, please contact us