Amytracker 520 is our green fluorescent optotracer molecule for labeling protein aggregates with repetitive arrangement of β-sheets. It labels Aβ plaques and neurofibrillary tangles in tissue sections with AD pathology and α-synuclein aggregates in tissue sections with PD pathology. Contact us to learn more about Amytracker applications.

As all our optotracers, Amytracker 520 is exceptionally photostable and fluorogenic. When bound to a target, Amytracker 520 can be imaged using epifluorescence, confocal and superresolution microscopy. Spectral information can be acquired using a fluorescence spectrophotometer. Use recommended filter sets as well as excitation- and emission wavelengths according to the following table.

Table: Excitation- and emission wavelengths as well as recommended filter sets.

Amytracker 520 is available as a 1 mg/ml solution in volumes ranging from 10 – 200 µL (see volumes and prices in the list below). As our standard solvent, we use ultrapure water. Please contact us for custom options.

Amytracker 540 is our yellow fluorescent optotracer molecule for labeling protein aggregates with repetitive arrangement of β-sheets. It labels Aβ plaques and neurofibrillary tangles in tissue sections with AD pathology and α-synuclein aggregates in tissue sections with PD pathology. Contact us to learn more about Amytracker applications.

As all our optotracers, Amytracker 540 is exceptionally photostable and fluorogenic. When bound to a target, Amytracker 540 can be imaged using epifluorescence, confocal and superresolution microscopy. Spectral information can be acquired using a fluorescence spectrophotometer. Use recommended filter sets as well as excitation- and emission wavelengths according to the following table.
Table: Excitation- and emission wavelengths as well as recommended filter sets.

Amytracker 540 is available as a 1 mg/ml solution in volumes ranging from 10 – 200 µL (see volumes and prices in the list below). As our standard solvent, we use ultrapure water. Please contact us for custom options.

Amytracker 630 is our orange fluorescent optotracer for labeling protein aggregates with repetitive arrangement of β-sheets. It labels Aβ plaques and neurofibrillary tangles in tissue sections with AD pathology and α-synuclein aggregates in tissue sections with PD pathology. Contact us to learn more about Amytracker applications.

As all our optotracers, Amytracker 630 is exceptionally photostable and fluorogenic. When bound to a target, Amytracker 630 can be imaged using epifluorescence, confocal and superresolution microscopy. Spectral information can be acquired using a fluorescence spectrophotometer. Use recommended filter sets as well as excitation- and emission wavelengths according to the following table.
Table: Excitation- and emission wavelengths as well as recommended filter sets.

Amytracker 630 is available as a 1 mg/ml solution in volumes ranging from 10 – 200 µL (see volumes and prices in the list below). As our standard solvent, we use ultrapure water. Please contact us for custom options.

Amytracker 680 is our red fluorescent optotracer for labeling protein aggregates with repetitive arrangement of β-sheets. It labels Aβ plaques and neurofibrillary tangles in tissue sections with AD pathology and α-synuclein aggregates in tissue sections with PD pathology. Specifically, Amytracker 680 has been used to study amyloid formation during abnormal coagulation, Lewy body formation in a seeding based neuronal model, accumulation of misfolded proteins in the nucleolus and intracerebral formation of Aβ plaques using multiphoton microscopy. Contact us to learn more about Amytracker applications.

As all our optotracers, Amytracker 680 is exceptionally photostable and fluorogenic. When bound to a target, Amytracker 680 can be imaged using epifluorescence, confocal and superresolution microscopy. Spectral information can be acquired using a fluorescence spectrophotometer. Use recommended filter sets as well as excitation- and emission wavelengths according to the following table.
Table: Excitation- and emission wavelengths as well as recommended filter sets.

Amytracker 680 is available as a 1 mg/ml solution in volumes ranging from 10 – 200 µL (see volumes and prices in the list below). As our standard solvent, we use ultrapure water (MilliQ). We also provide Amytracker 680 dissolved in DMSO. Please contact us for custom options.

Amytracker Mix&Try Kit is our recommended option for starting out with using Amytracker. It contains a sample volume (10 µL) of each variant. Testing each variant will allow you to determine which Amytracker is best suited for your experiments and available instruments.

All Amytracker variants label Aβ plaques and neurofibrillary tangles in tissue sections with AD pathology and α-synuclein aggregates in tissue sections with PD pathology. The optotracers are exceptionally photostable and fluorogenic. The variants differ with regards to affinity, cellular uptake and excitation and emission wavelengths (see Table below). When bound to a target, Amytracker can be imaged using epifluorescence, confocal and superresolution microscopy. Spectral information can be acquired using a fluorescence spectrophotometer. Contact us to learn more about Amytracker applications.

Table: Excitation- and emission wavelengths as well as recommended filter sets.

Carbotrace 520 is our green fluorescent optotracer molecule for labeling glucans and some hemicelluloses. Generally, labeling is strongest with homopolymers compared to highly branched polysaccharides. Optotracers are structure-responsive molecules which display a unique spectral signature when binding to a target structure. Therefore, depending on the sample composition, it is possible to determine the purity of cellulose in the sample. Contact us to learn more about applications for Carbotrace 520.

Table: Excitation- and emission wavelengths as well as recommended filter sets.

Carbotrace 520 is available as a 1 mg/ml solution in volumes ranging from 10 – 200 µL (see volumes and prices in the list below). As our standard solvent, we use ultrapure water. Please contact us for custom options.

Carbotrace 540 is our yellow fluorescent optotracer molecule for labeling glucans and some hemicelluloses. Generally, labeling is strongest with homopolymers compared to highly branched polysaccharides. Optotracers are structure-responsive molecules which display a unique spectral signature when binding to a target structure. Therefore, depending on the sample composition, it is possible to determine the purity of cellulose in the sample.

Specifically, Carbotrace 540 has been used to determine the purity of cellulose extracted from macroalgae by allowing analysis of extracted carbohydrates in their polymeric state. In addition, Carbotrace 540 has shown to bind to the hemicellulose xyloglucan. Contact us to learn more about applications for Carbotrace 540.

Table: Excitation- and emission wavelengths as well as recommended filter sets.

Carbotrace 540 is available as a 1 mg/ml solution in volumes ranging from 10 – 200 µL (see volumes and prices in the list below). As our standard solvent, we use ultrapure water. Please contact us for custom options.

Carbotrace 630 is our orange fluorescent optotracer molecule for labeling glucans and some hemicelluloses. Generally, labeling is strongest with homopolymers compared to highly branched polysaccharides. Optotracers are structure-responsive molecules which display a unique spectral signature when binding to a target structure. Therefore, depending on the sample composition, it is possible to determine the purity of cellulose in the sample. Contact us to learn more about applications for Carbotrace 630.

Table: Excitation- and emission wavelengths as well as recommended filter sets.

Carbotrace 630 is available as a 1 mg/ml solution in volumes ranging from 10 – 200 µL (see volumes and prices in the list below). As our standard solvent, we use ultrapure water. Please contact us for custom options.

Carbotrace 680 is our red fluorescent optotracer molecule for labeling glucans and some hemicelluloses. Generally, labeling is strongest with homopolymers compared to highly branched polysaccharides. Optotracers are structure-responsive molecules which display a unique spectral signature when binding to a target structure. Therefore, depending on the sample composition, it is possible to determine the purity of cellulose in the sample.

Specifically, Carbotrace 680 has been used to image cellulose and starch components in plant cells and differentiate between those components using multi-laser/multi-detector analysis with confocal microscopy. Using a fluorescence spectrophotometer to acquire excitation and emission spectra, a unique spectral signature allows to differentiate between α(1-3), α(1-4), α(1-6), β(1-3), β(1-4) and β(1-6) linked glucans. As such, it labels cell wall components such as Cellulose, Chitin, Laminarin and the hemicellulose xyloglucan. Further, Carbotrace 680 has been used to determine relative carbohydrate content and elasticity of cell walls in plant material. Contact us to learn more about applications for Carbotrace 680.

Table: Excitation- and emission wavelengths as well as recommended filter sets.

Carbotrace 680 is available as a 1 mg/ml solution in volumes ranging from 10 – 200 µL (see volumes and prices in the list below). As our standard solvent, we use ultrapure water (MilliQ). We also provide Carbotrace 680 dissolved in DMSO Please contact us for custom options.

Carbotrace Mix&Try Kit is our recommended option for starting out with using our fluorescent optotracers. It contains a sample volume (10 µL) of each variant. Testing each variant will allow you to determine which Carbotrace is best suited for your experiments and available instruments.

All Carbotrace variants label glucans and some hemicelluloses. Generally, labeling is strongest with homopolymers compared to highly branched polysaccharides. The optotracers are structure-responsive molecules which display a unique spectral signature when binding to a target structure. Therefore, depending on the sample composition, it is possible to determine the purity of cellulose in the sample. Contact us to learn more about Carbotrace applications.

Table: Excitation- and emission wavelengths as well as recommended filter sets.

EbbaBiolight 520 is our green fluorescent optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Contact us to learn more about EbbaBiolight applications.

As all our optotracers, EbbaBiolight 520 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 520 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below. EbbaBiolight are intended for use in live-cultures and are not compatible with fixation. If fixation needs to be performed, we recommend to apply fixation after labelling.

Table: Excitation- and emission wavelengths as well as recommended filter sets.

EbbaBiolight 520 is available as a 1 mg/ml solution in volumes ranging from 10 – 200 µL (see volumes and prices in the list below). As our standard solvent, we use ultrapure water. Please contact us for custom options.

EbbaBiolight 540 is our yellow fluorescent optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Contact us to learn more about EbbaBiolight applications.

As all our optotracers, EbbaBiolight 540 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 540 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below. EbbaBiolight are intended for use in live-cultures and are not compatible with fixation. If fixation needs to be performed, we recommend to apply fixation after labelling.

Table: Excitation- and emission wavelengths as well as recommended filter sets.

EbbaBiolight 540 is available as a 1 mg/ml solution in volumes ranging from 10 – 200 µL (see volumes and prices in the list below). As our standard solvent, we use ultrapure water. Please contact us for custom options.

EbbaBiolight 630 is our orange fluorescent optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, EbbaBiolight 630 labels peptidoglycan and lipoteichoic acids in the cell wall of certain gram positives like Staphylococci and E. faecalis. Contact us to learn more about EbbaBiolight applications.

As all our optotracers, EbbaBiolight 630 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 630 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below. EbbaBiolight are intended for use in live-cultures and are not compatible with fixation. If fixation needs to be performed, we recommend to apply fixation after labelling.

Table: Excitation- and emission wavelengths as well as recommended filter sets.

EbbaBiolight 630 is available as a 1 mg/ml solution in volumes ranging from 10 – 200 µL (see volumes and prices in the list below). As our standard solvent, we use ultrapure water. Please contact us for custom options.

EbbaBiolight 680 is our red fluorescent optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, EbbaBiolight 680 labels curli in the extracellular matrix produced by Salmonella as well as β-glucans and chitins in yeast- and hyphal forms of C. albicans. Furthermore, it has been used to track pellicle biofilm formation by B. cenocepacia. Contact us to learn more about EbbaBiolight applications.

As all our optotracers, EbbaBiolight 680 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 680 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below. EbbaBiolight are intended for use in live-cultures and are not compatible with fixation. If fixation needs to be performed, we recommend to apply fixation after labelling.

Table: Excitation- and emission wavelengths as well as recommended filter sets.

EbbaBiolight 680 is available as a 1 mg/ml solution in volumes ranging from 10 – 200 µL (see volumes and prices in the list below). AAs our standard solvent, we use ultrapure water (MilliQ). We also provide EbbaBiolight 680 dissolved in DMSO Please contact us for custom options.

EbbaBiolight Mix&Try Kit is our recommended option for starting out with using EbbaBiolight. It contains a sample volume (10 µL) of each variant. Testing each variant in the EbbaBiolight Mix&Try Kit will allow you to determine which one of our optotracers is best suited for your experiment.

All EbbaBiolight variants label extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, the optotracers have been used as follows: EbbaBiolight 480 labeling extracellular matrix produced by P. aeruginosa but not C. albicans in a mircofluidic device during mono- and co-culture. EbbaBiolight 630 labeling peptidoglycan and lipoteichoic acids in the cell wall of certain gram positives like Staphylococci and E. faecalis. EbbaBiolight 680 labeling curli in the extracellular matrix produced by Salmonella as well as β-glucans, chitin and intracellular amyloid aggregates in yeast- and hypal forms of C. albicans. Furthermore, it has been used to track pellicle biofilm formation in B. cenocepacia. Contact us to learn more about EbbaBiolight applications.

All EbbaBiolight variants are exceptionally photostable and fluorogenic. When bound to a target, the optotracers can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Excitation and emission wavelengths for each molecule are shown in Table 1. EbbaBiolight are intended for use in live-cultures and are not compatible with fixation. If fixation needs to be performed, we recommend to apply fixation after labelling.

Table 1: Excitation- and emission wavelengths as well as recommended filter sets.