INDIGO Biosciences, Inc. is a leading provider of nuclear receptor and in vitro toxicology solutions that accelerate scientific decision-making. We supplement the world’s largest portfolio of nuclear receptor kits and services and in vitro toxicology solutions with greater results readability, reproducibility, and faster turnaround times. Our solutions, plus supportive team and reliable science and platforms, aim to reduce the time, cost, and risk associated with the discovery process.


Cell-based Reporter Assays That Work Simply And Simply Work

Whether you are a seasoned expert or new to cell-based assays, achieve superior sensitivity and reproducible results in as little as 24 hours. No cell culture required.

Our kits and services get you on the fast track to data.

NEW PRODUCTS

Skip Cell Culture With All-Inclusive, Luciferase Reporter Assay Kits

Explore our extensive portfolio of receptor reporter assay kits, designed and optimized to provide extreme sensitivity and reproducible results so you can have confidence in your data.

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Nuclear Receptor Assay Kits: Extensive Selection and Quick Turnarounds

Accelerate decisions at the bench and for your business with our assays. No cell culture required. With the world’s largest library of nuclear receptor assays, you can:

  • Profile chemical effects on a specific nuclear receptor or full panels in days.
  • Unravel agonist and antagonist potentials of known and new substances quickly.
  • Evaluate your compounds’ efficacy, potency, and selectivity in record time.
  • Easily perform cross-species comparisons with our ortholog kits.
Description Catalog # Species Target Type Associated Disease
AhR Bioassay for Environmental Sample Monitoring EM06001 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Androgen Receptor Bioassay for Environmental Sample Monitoring EM03001 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Cyn Monkey FXR Reporter Assay Kit C00601 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Cyn Monkey PGR Reporter Assay Kit C05001 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Obesity, Osteoporosis, Reproduction
Cyn Monkey PPARa Reporter Assay Kit C00111 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Cyn Monkey PPARd Reporter Assay Kit C00121 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Cyn Monkey PPARg Reporter Assay Kit C00101 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Cyn Monkey PXR Reporter Assay Kit C07001 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Dog FXR Reporter Assay Kit D00601 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Dog LXRa Reporter Assay Kit D00311 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, NASH/NAFLD, Reproduction
Dog LXRb Reporter Assay Kit D00301 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dyslipidemia, Reproduction
Dog PPARa Reporter Assay Kit D00111 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Dog PPARd Reporter Assay Kit D00121 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Dog PXR Reporter Assay Kit D07001 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Estrogen Receptor Bioassay for Environmental Sample Monitoring EM00431 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Osteoporosis, Reproduction, Wound Healing
Glucocorticoid Receptor Bioassay for Environmental Sample Monitoring EM00201 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human AhR Reporter Assay Kit IB06001 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Human AR Reporter Assay Kit IB03001 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Human CAR-2 Reporter Assay Kit IB00921 Human Nuclear Hormone Receptor NASH/NAFLD, Wound Healing
Human CAR-3 Reporter Assay Kit IB00901 Human Nuclear Hormone Receptor NASH/NAFLD, Wound Healing
Human ERa Reporter Assay Kit IB00401 Human Nuclear Hormone Receptor Autoimmune, Cardiovascular, Dyslipidemia, NASH/NAFLD, Obesity, Osteoporosis, Reproduction, Wound Healing
Human ERb Reporter Assay Kit IB00411 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Osteoporosis, Reproduction, Wound Healing
Human ERRa Reporter Assay Kit IB08001 Human Nuclear Hormone Receptor Cancer
Human ERRb Reporter Assay Kit IB08011 Human Nuclear Hormone Receptor Cancer
Human ERRg Reporter Assay Kit IB08021 Human Nuclear Hormone Receptor Cancer
Human FXR Reporter Assay Kit IB00601 Human Nuclear Hormone Receptor Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Human GR Reporter Assay Kit IB00201 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human LXRa Reporter Assay Kit IB00311 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, NASH/NAFLD, Reproduction
Human LXRb Reporter Assay Kit IB00301 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dyslipidemia, Reproduction
Human MR Reporter Assay Kit IB00501 Human Nuclear Hormone Receptor Cardiovascular, Dyslipidemia, Kidney Disease, Osteoporosis
Human Nrf2 Reporter Assay Kit IB10001 Human Nuclear Hormone Receptor Cancer, Cardiovascular, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction
Human PGR Reporter Assay Kit IB05001 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Obesity, Osteoporosis, Reproduction
Human PPARa Reporter Assay Kit IB00111 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Human PPARd Reporter Assay Kit IB00121 Human Nuclear Hormone Receptor Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Human PPARg Reporter Assay Kit IB00101 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human PXR Reporter Assay Kit IB07001 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Human RARa Reporter Assay Kit IB02201 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RARb Reporter Assay Kit IB02101 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RARg Reporter Assay Kit IB02001 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RORa Reporter Assay Kit IB04011 Human Nuclear Hormone Receptor Cancer, Dyslipidemia, NASH/NAFLD
Human RORg Reporter Assay Kit IB04001 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, NASH/NAFLD, Obesity
Human RXRa Reporter Assay Kit IB00801 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RXRb Reporter Assay Kit IB00811 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RXRg Reporter Assay Kit IB00821 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Dyslipidemia, Obesity, Wound Healing
Human TRa Reporter Assay Kit IB01001 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Human TRb Reporter Assay Kit IB01101 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Human VDR Reporter Assay Kit IB00701 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, Obesity, Osteoporosis, Reproduction
Mineralocorticoid Receptor Bioassay for Environmental Sample Monitoring EM00501 Human Nuclear Hormone Receptor Cardiovascular, Dyslipidemia, Kidney Disease, Osteoporosis
Mouse AhR Reporter Assay Kit M06001 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Mouse CAR Reporter Assay Kit M00901 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs NASH/NAFLD, Wound Healing
Mouse FXR Reporter Assay Kit M00601 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Mouse GR Reporter Assay Kit M00201 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Mouse LXRa Reporter Assay Kit M00311 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, NASH/NAFLD, Reproduction
Mouse LXRb Reporter Assay Kit M00301 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dyslipidemia, Reproduction
Mouse PPARa Reporter Assay Kit M00111 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Mouse PPARd Reporter Assay Kit M00121 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Mouse PXR Reporter Assay Kit M07001 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Mouse RORg Reporter Assay Kit M04001 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Dermatitis, NASH/NAFLD, Obesity
Mouse/Rat PPARg Reporter Assay Kit MR00101 Rodent (Mouse / Rat shared ortholog) Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Panel of Human ER Reporter Assays: ERa & ERb IB00421-48P Human Nuclear Hormone Receptor
Panel of Human LXR Reporter Assays: LXRa & LXRb IB00321-48P Human Nuclear Hormone Receptor
Panel of Human PPAR Reporter Assays: PPARa, PPARd, & PPARg IB00131-32P Human Nuclear Hormone Receptor
Panel of Human RAR Reporter Assays: RARa, RARb & RARg IB02301-32P Human Nuclear Hormone Receptor
Panel of Human RXR Reporter Assays: RXRa, RXRb & RXRg IB00831-32P Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Panel of Human TR Reporter Assays: TRa & TRb IB01201-48P Human Nuclear Hormone Receptor
Rabbit PGR Reporter Assay Kit RB05001 Rabbit Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Obesity, Osteoporosis, Reproduction
Rat AhR Reporter Assay Kit R06001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Rat AR Reporter Assay Kit R03001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Rat ERa Reporter Assay Kit R00401 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cardiovascular, Dyslipidemia, NASH/NAFLD, Obesity, Osteoporosis, Reproduction, Wound Healing
Rat ERb Reporter Assay Kit R00411 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Osteoporosis, Reproduction, Wound Healing
Rat FXR Reporter Assay Kit R00601 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Rat GR Reporter Assay Kit R00201 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Rat LXRb Reporter Assay Kit R00301 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dyslipidemia, Reproduction
Rat LXRα Reporter Assay Kit R00311 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, NASH/NAFLD, Reproduction
Rat PGR Reporter Assay Kit R05001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Obesity, Osteoporosis, Reproduction
Rat PPARa Reporter Assay Kit R00111 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Rat PPARd Reporter Assay Kit R00121 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Rat PXR Reporter Assay Kit R07001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Rat RORγ Reporter Assay Kit R04001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Dermatitis, NASH/NAFLD, Obesity
Zebrafish AhR Reporter Assay Kit Z06001 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Zebrafish AR Reporter Assay Kit Z03001 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Zebrafish ERa Reporter Assay Kit Z00401 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Osteoporosis, Reproduction, Wound Healing
Zebrafish GR Reporter Assay Kit Z00201 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Zebrafish PPARg Reporter Assay Kit Z00101 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Zebrafish RARa isoform A Reporter Assay Kit Z02201 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Zebrafish TRb Reporter Assay Kit Z01101 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction

Frequently Asked Questions

Do I need any special equipment to utilize INDIGO's assay kits?

Each kit includes luciferase reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), and a detailed Technical Manual. A luminometer is required for Relative Light Unit (RLU) measurements.

Do INDIGO's Nuclear Receptor Assay Kits use stable cell lines or transiently transfected cells?

INDIGO’s Nuclear Receptor Assay Kits use transiently transfected cells, less prone to genetic drift and batch-to-batch variations. Moreover, they do not require cell culture, significantly reducing time and contamination risks. Simply thaw the reporter cells, and you are ready to perform the assay.

Can INDIGO's Nuclear Receptor Assay Kits be used for diagnostic purposes?

INDIGO’s Nuclear Receptor Assay Kits are not for diagnostic purposes.

Can I refreeze or reuse Reporter Cells or INDIGlo Luciferase Detection Reagent?

Luciferase Reporter Cells are single-use reagents, regardless of the assay format. Once thawed, Reporter Cells can not be refrozen or reused. Assay kits provide sufficient extra volumes of all reagents to accommodate the needs of performing mechanical transfers and test compound dilutions. However, extra volumes of reporter cells and Luciferase Detection Reagent should be discarded after each assay set-up.

Which wavelength should I use to read my plate?

INDIGO’s assays are luminescence-based, not fluorescence-based. Therefore, one does not designate specific wavelengths (or filters of any kind) when reading in luminescence mode. Total photon emission is quantified.

Ortholog Assays: Maximize the Potential of Animal Studies

INDIGO Biosciences offers 40+ ortholog assays to streamline animal studies and aid drug development.

  • Facilitate cross-species comparisons in animal studies
  • Select the most suitable animal model as a surrogate for humans
  • Enable quicker assessment with confident data interpretation
Description Catalog # Species Target Type Associated Disease
Cyn Monkey FXR Reporter Assay Kit C00601 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Cyn Monkey PGR Reporter Assay Kit C05001 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Obesity, Osteoporosis, Reproduction
Cyn Monkey PPARa Reporter Assay Kit C00111 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Cyn Monkey PPARd Reporter Assay Kit C00121 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Cyn Monkey PPARg Reporter Assay Kit C00101 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Cyn Monkey PXR Reporter Assay Kit C07001 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Dog FXR Reporter Assay Kit D00601 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Dog LXRa Reporter Assay Kit D00311 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, NASH/NAFLD, Reproduction
Dog LXRb Reporter Assay Kit D00301 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dyslipidemia, Reproduction
Dog PPARa Reporter Assay Kit D00111 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Dog PPARd Reporter Assay Kit D00121 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Dog PXR Reporter Assay Kit D07001 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Mouse AhR Reporter Assay Kit M06001 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Mouse CAR Reporter Assay Kit M00901 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs NASH/NAFLD, Wound Healing
Mouse FXR Reporter Assay Kit M00601 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Mouse GR Reporter Assay Kit M00201 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Mouse LXRa Reporter Assay Kit M00311 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, NASH/NAFLD, Reproduction
Mouse LXRb Reporter Assay Kit M00301 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dyslipidemia, Reproduction
Mouse PPARa Reporter Assay Kit M00111 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Mouse PPARd Reporter Assay Kit M00121 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Mouse PXR Reporter Assay Kit M07001 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Mouse RORg Reporter Assay Kit M04001 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Dermatitis, NASH/NAFLD, Obesity
Mouse/Rat PPARg Reporter Assay Kit MR00101 Rodent (Mouse / Rat shared ortholog) Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Rabbit PGR Reporter Assay Kit RB05001 Rabbit Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Obesity, Osteoporosis, Reproduction
Rat AhR Reporter Assay Kit R06001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Rat AR Reporter Assay Kit R03001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Rat ERa Reporter Assay Kit R00401 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cardiovascular, Dyslipidemia, NASH/NAFLD, Obesity, Osteoporosis, Reproduction, Wound Healing
Rat ERb Reporter Assay Kit R00411 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Osteoporosis, Reproduction, Wound Healing
Rat FXR Reporter Assay Kit R00601 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Rat GR Reporter Assay Kit R00201 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Rat LXRb Reporter Assay Kit R00301 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dyslipidemia, Reproduction
Rat LXRα Reporter Assay Kit R00311 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, NASH/NAFLD, Reproduction
Rat PGR Reporter Assay Kit R05001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Obesity, Osteoporosis, Reproduction
Rat PPARa Reporter Assay Kit R00111 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Rat PPARd Reporter Assay Kit R00121 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Rat PXR Reporter Assay Kit R07001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Rat RORγ Reporter Assay Kit R04001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Dermatitis, NASH/NAFLD, Obesity
Zebrafish AhR Reporter Assay Kit Z06001 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Zebrafish AR Reporter Assay Kit Z03001 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Zebrafish ERa Reporter Assay Kit Z00401 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Osteoporosis, Reproduction, Wound Healing
Zebrafish GR Reporter Assay Kit Z00201 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Zebrafish PPARg Reporter Assay Kit Z00101 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Zebrafish RARa isoform A Reporter Assay Kit Z02201 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Zebrafish TRb Reporter Assay Kit Z01101 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction

Cross Species Comparisons with Ortholog Assays

While animal studies are widely used for evaluating chemical interactions with our biological systems, they present numerous challenges that significantly impede the development of drug candidates and can cause delays in moving to clinical trials. Recognizing these issues and the lack of solutions, INDIGO Biosciences has developed and validated over 40 ortholog nuclear receptor assays for a variety of commonly used laboratory animals such as rats, mice, dogs, monkeys, rabbits, and zebrafish.

By offering this unique portfolio of ortholog assays, INDIGO Biosciences facilitates cross-species comparisons, enabling researchers to effectively interpret the results obtained from previous animal trials or to select the most appropriate animal model as a surrogate for humans. Now, it is possible to assess differences in the activities of ortholog receptors compared to their human counterparts within a matter of days, allowing for quicker evaluation of drug-specific effects.

With our comprehensive selection of ortholog assays, you can confidently screen the appropriate animal model prior to conducting trials and interpret your data with certainty. Don’t leave anything to chance.

Frequently Asked Questions

Are INDIGO assays binding assays, or does INDIGO offer binding assays?

INDIGO’s assays are not binding assays. They are cell-based trans-activation assays, and the principal application is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that the compounds may exert against the nuclear receptors. INDIGO reporter systems utilize firefly luciferase reporter gene technology, and while there is a binding taking place, our assays do not measure it. Instead, the luciferase light response is measured which correlates to the activation status of the receptor (either activation or inhibition). Quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in receptor activity. It will tell the scientist the significance (strength) of the interaction by the level of light emitted. In addition, cell-based assays are more sensitive and able to detect smaller levels of activation.

What cell lines/types are used?

Do INDIGO’s nuclear receptor assays express the native, full-length receptor?

Reporter cells included in INIDIGO’s steroid hormone nuclear assay kits (ERα, ERβ, AR, PGR, MR, GR) express the native, full-length receptors. INDIGO’s other nuclear receptor assays, however, include reporter cells that express hybrid nuclear receptors. In these cases, the respective receptor’s native N-terminal sequence comprising the DNA Binding Domain (DBD) has been replaced with sequence encoding the yeast Gal4-DBD. All other native NR functional/structural domains (ligand binding domain, hinge region, and various activation domains) are present in these hybrid receptors. These reporter cells also contain the firefly luciferase reporter gene functionally linked to the upstream genetic response element for Gal4. Consequently, once a bioactive compound associates with the ligand binding domain of the hybrid receptor, only the luciferase reporter gene is induced. Ligand-activation of the hybrid receptor will not induce collateral expression of target genes that are otherwise regulated by the native nuclear receptor.

Toggle title Can you tell me what the reference agonist/antagonist is for your receptor assays?

View our list of reference compounds. We do utilize commercially available reference agonists for our assays, and this reference agonist is included in each assay kit. We do use reference antagonists where a reference is known and commercially available. Some of our antagonist assays do not have reference antagonists available.

Do I need any special equipment to utilize INDIGO’s assay kits?

Each kit includes luciferase reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), and a detailed Technical Manual. A luminometer is required for Relative Light Unit (RLU) measurements.

Can INDIGO’s Nuclear Receptor Assay Kits be used for diagnostic purposes?

INDIGO’s kits are intended for research purposes only, and not for diagnostic or therapeutic use in humans.

Growth Factor Receptor Assay Kits: Accelerate Your Research

Accelerate decisions at the bench and for your business with our family of Growth Factor Assay Kits. No cell culture required.

  • Accurate quantification of even low levels of growth factors with exceptional sensitivity
  • With user-friendly protocols and streamlined workflows, our assay kits enable effortless implementation
  • Backed by rigorous quality control measures and optimized reagent formulations, our assay kits ensure reproducible and reliable data
Description Sizes Catalog # Species Target Type Associated Disease
FGFR/β-Klotho Reporter Assay Kit 1 x 96-well format assays IB22001 Human Growth Factor Receptor Cardiovascular, NASH/NAFLD, Obesity, Reproduction, Wound Healing
FGFR/β-Klotho Reporter Assay Kit 3 x 32 assays in 96-well format IB22001-32 Human Growth Factor Receptor Cardiovascular, NASH/NAFLD, Obesity, Reproduction, Wound Healing
FGFR/β-Klotho Reporter Assay Kit 1 x 384-well format assays IB22002 Human Growth Factor Receptor Cardiovascular, NASH/NAFLD, Obesity, Reproduction, Wound Healing
FGFR1/2 Reporter Assay Kit 1 x 96-well format assays IB21001 Human Growth Factor Receptor Autoimmune, Cancer, Kidney Disease, NASH/NAFLD, Neurodegenerative, Reproduction, Wound Healing
FGFR1/2 Reporter Assay Kit 3 x 32 assays in 96-well format IB21001-32 Human Growth Factor Receptor Autoimmune, Cancer, Kidney Disease, NASH/NAFLD, Neurodegenerative, Reproduction, Wound Healing
FGFR1/2 Reporter Assay Kit 1 x 384-well format assays IB21002 Human Growth Factor Receptor Autoimmune, Cancer, Kidney Disease, NASH/NAFLD, Neurodegenerative, Reproduction, Wound Healing
Human EGFR1 Reporter Assay Kit 1 x 96-well format assays IB13001 Human Growth Factor Receptor Cancer, Dermatitis, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Human EGFR1 Reporter Assay Kit 3 x 32 assays in 96-well format IB13001-32 Human Growth Factor Receptor Cancer, Dermatitis, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Human EGFR1 Reporter Assay Kit 1 x 384-well format assays IB13002 Human Growth Factor Receptor Cancer, Dermatitis, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Human GHR Reporter Assay Kit 1 x 96-well format assays IB14001 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human GHR Reporter Assay Kit 3 x 32 assays in 96-well format IB14001-32 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human GHR Reporter Assay Kit 1 x 384-well format assays IB14002 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human IGF-1R Reporter Assay Kit 1 x 96-well format assays IB29001 Human Growth Factor Receptor Cancer, Obesity, Reproduction
Human IGF-1R Reporter Assay Kit 1 x 384-well format assays IB29002 Human Growth Factor Receptor Cancer, Obesity, Reproduction
Human INSRb Reporter Assay Kit 1 x 96-well format assays IB28001 Human Growth Factor Receptor Obesity, Reproduction
Human INSRb Reporter Assay Kit 1 x 384-well format assays IB28002 Human Growth Factor Receptor Obesity, Reproduction
Human PDGFR a/b Reporter Assay Kit 1 x 96-well format assays IB23001 Human Growth Factor Receptor Wound Healing
Human PDGFR a/b Reporter Assay Kit 3 x 32 assays in 96-well format IB23001-32 Human Growth Factor Receptor Wound Healing
Human PDGFR a/b Reporter Assay Kit 1 x 384-well format assays IB23002 Human Growth Factor Receptor Wound Healing
Human TGFbR Reporter Assay Kit 1 x 96-well format assays IB12001 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Wound Healing
Human TGFbR Reporter Assay Kit 3 x 32 assays in 96-well format IB12001-32 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Wound Healing
Human TGFbR Reporter Assay Kit 1 x 384-well format assays IB12002 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Wound Healing
Human TrkA Reporter Assay Kit 1 x 96-well format assays IB27011 Human Growth Factor Receptor Autoimmune, Cancer, Obesity
Human TrkA Reporter Assay Kit 1 x 384-well format assays IB27012 Human Growth Factor Receptor Autoimmune, Cancer, Obesity
Human TrkB Reporter Assay Kit 1 x 96-well format assays IB27021 Human Growth Factor Receptor Autoimmune, Cancer, Neurodegenerative, Reproduction
Human TrkB Reporter Assay Kit 1 x 384-well format assays IB27022 Human Growth Factor Receptor Autoimmune, Cancer, Neurodegenerative, Reproduction
Human TrkC Reporter Assay Kit 1 x 96-well format assays IB27031 Human Growth Factor Receptor Cancer, Neurodegenerative
Human TrkC Reporter Assay Kit 1 x 384-well format assays IB27032 Human Growth Factor Receptor Cancer, Neurodegenerative
Human VEGFR Reporter Assay Kit 1 x 96-well format assays IB15001 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction
Human VEGFR Reporter Assay Kit 3 x 32 assays in 96-well format IB15001-32 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction
Human VEGFR Reporter Assay Kit 1 x 384-well format assays IB15002 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction

Growth Factor Receptors

Growth factor receptors are the initial step in a cell’s signaling cascade for cell differentiation and proliferation. The growth signal is initially carried by receptor ligands which then bind to cell-surface receptor tyrosine kinases (RTKs). Growth factor receptors utilize the JAK/STAT, PI3 kinase, and MAP kinase pathways, as well as transcription factors like signal transducers or SMAD proteins. While growth factors act on different cell types, their signal pathways often overlap. This shared mechanism has generated a significant amount of interest in cancer research.

Because of their effect on cell growth, research surrounding growth factor receptors typically focuses on their ability to pinpoint cancer treatments. Once growth factors bind to their receptor, a signal transduction pathway is activated within the cell to ensure the cell is functioning properly. In cancer cells, however, this pathway may never turn on or off. In certain cancers, these receptors are often overexpressed, corresponding to uncontrolled proliferation or differentiation. In addition, the expression of mutant forms of growth factor proteins may also lead to cancer.

Frequently Asked Questions

Are INDIGO assays binding assays, or does INDIGO offer binding assays?

INDIGO’s assays are not binding assays. They are cell-based trans-activation assays, and the principal application is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that the compounds may exert against the nuclear receptors. INDIGO reporter systems utilize firefly luciferase reporter gene technology, and while there is a binding taking place, our assays do not measure it. Instead, the luciferase light response is measured which correlates to the activation status of the receptor (either activation or inhibition). Quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in receptor activity. It will tell the scientist the significance (strength) of the interaction by the level of light emitted. In addition, cell-based assays are more sensitive and able to detect smaller levels of activation.

What cell lines/types are used?

INDIGO’s nuclear receptor assays utilize proprietary human and non-human mammalian cells engineered to provide constitutive, high-level expression of the designated receptor. Specific cell type information for each assay is proprietary and available only through consultation with INDIGO’s technical team following a screening service or assay kit purchase.

Do INDIGO’s nuclear receptor assays express the native, full-length receptor?

Reporter cells included in INIDIGO’s steroid hormone nuclear assay kits (ERα, ERβ, AR, PGR, MR, GR) express the native, full-length receptors. INDIGO’s other nuclear receptor assays, however, include reporter cells that express hybrid nuclear receptors. In these cases, the respective receptor’s native N-terminal sequence comprising the DNA Binding Domain (DBD) has been replaced with sequence encoding the yeast Gal4-DBD. All other native NR functional/structural domains (ligand binding domain, hinge region, and various activation domains) are present in these hybrid receptors. These reporter cells also contain the firefly luciferase reporter gene functionally linked to the upstream genetic response element for Gal4. Consequently, once a bioactive compound associates with the ligand binding domain of the hybrid receptor, only the luciferase reporter gene is induced. Ligand-activation of the hybrid receptor will not induce collateral expression of target genes that are otherwise regulated by the native nuclear receptor.

Can you tell me what the reference agonist/antagonist is for your receptor assays?

View our list of reference compounds. We do utilize commercially available reference agonists for our assays, and this reference agonist is included in each assay kit. We do use reference antagonists where a reference is known and commercially available. Some of our antagonist assays do not have reference antagonists available.

Do I need any special equipment to utilize INDIGO’s assay kits?

Each kit includes luciferase reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), and a detailed Technical Manual. A luminometer is required for Relative Light Unit (RLU) measurements.

Can INDIGO’s Nuclear Receptor Assay Kits be used for diagnostic purposes?

INDIGO’s kits are intended for research purposes only, and not for diagnostic or therapeutic use in humans.

GPCR Assay Kits: Streamline Your GPCR Research

Illuminate cellular signaling with our GPCR Assay Kits:

  • Uncover subtle changes in cellular signaling pathways through quantification of GPCR activity
  • Enable confident decision-making with robust and reliable results
Description Sizes Catalog # Species Target Type Associated Disease
CB1R Reporter Assay Kit 1 x 96-well format assays IB19001 Human GPCR Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis
CB1R Reporter Assay Kit 3 x 32 assays in 96-well format IB19001-32 Human GPCR Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis
CB1R Reporter Assay Kit 1 x 384-well format assays IB19002 Human GPCR Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis
Human ADRA1A Reporter Assay Kit 1 x-96 well format assays IB31001 Human GPCR Cardiovascular, Dyslipidemia, Neurodegenerative
Human ADRA1A Reporter Assay Kit 1 x-384 well format assays IB31002 Human GPCR Cardiovascular, Dyslipidemia, Neurodegenerative
Human ADRA1B Reporter Assay Kit 1 x-96 well format assays IB31101 Human GPCR Cardiovascular, Dyslipidemia, Neurodegenerative
Human ADRA1B Reporter Assay Kit 1 x-384 well format assays IB31102 Human GPCR Cardiovascular, Dyslipidemia, Neurodegenerative
Human ADRA1D Reporter Assay Kit 1 x-96 well format assays IB31201 Human GPCR Cardiovascular, Dyslipidemia, Neurodegenerative
Human ADRA1D Reporter Assay Kit 1 x-384 well format assays IB31202 Human GPCR Cardiovascular, Dyslipidemia, Neurodegenerative
Human ADRA2A Reporter Assay Kit 1 x-96 well format assays IB34001 Human GPCR Cancer, Cardiovascular
Human ADRA2A Reporter Assay Kit 1 x-384 well format assays IB34002 Human GPCR Cancer, Cardiovascular
Human ADRB1 Reporter Assay Kit 1 x-96 well format assays IB32001 Human GPCR Cancer, Cardiovascular, Dyslipidemia
Human ADRB1 Reporter Assay Kit 1 x-384 well format assays IB32002 Human GPCR Cancer, Cardiovascular, Dyslipidemia
Human ADRB2 Reporter Assay Kit 1 x-96 well format assays IB32101 Human GPCR  
Human ADRB2 Reporter Assay Kit 1 x-384 well format assays IB32102 Human GPCR  
Human AVPR1A Reporter Assay Kit 1 x-96 well format assays IB37001 Human GPCR  
Human AVPR1A Reporter Assay Kit 1 x-384 well format assays IB37002 Human GPCR  
Human GIPR Reporter Assay Kit 1 x-96 well format assays IB38001 Human GPCR Obesity
Human GIPR Reporter Assay Kit 1 x-384 well format assays IB38002 Human GPCR Obesity
Human GLP-1R Reporter Assay Kit 1 x-96 well format assays IB33001 Human GPCR Obesity
Human GLP-1R Reporter Assay Kit 1 x-384 well format assays IB33002 Human GPCR Obesity
Human OXTR Reporter Assay Kit 1 x-96 well format assays IB35001 Human GPCR  
Human OXTR Reporter Assay Kit 1 x-384 well format assays IB35002 Human GPCR  
Human TGR5 Reporter Assay Kit 1 x 96-well format assays IB26001 Human GPCR Cardiovascular
Human TGR5 Reporter Assay Kit 1 x 384-well format assays IB26002 Human GPCR Cardiovascular

Unveiling the Significance of GPCRs: Key Players in Cellular Signaling and Therapeutic Potential

GPCRs, short for G-protein coupled receptors, are a vital class of cell surface receptors involved in various physiological processes. These receptors transmit extracellular signals to the intracellular environment, influencing cellular behavior and regulation of important functions.

GPCRs are incredibly diverse and exist in abundance throughout the human body. They play crucial roles in the molecular sensing of hormones, neurotransmitters, and light, making them vital to sensory processes such as vision, olfaction, and taste.

The basic structure of GPCRs consists of seven transmembrane helices embedded in the cellular membrane. When a ligand binds to the receptor, it triggers a series of events leading to the activation of intracellular signaling pathways. These pathways, often mediated by G-proteins, can impact gene expression, enzyme activity, ion channel conductance, and other cellular responses.

Understanding GPCRs is crucial for drug development, as they are targets for a significant proportion of medications on the market today. Exploring the intricacies of these receptors continues to be an exciting area of research, offering promising avenues for novel therapeutic interventions.

Frequently Asked Questions

Are INDIGO assays binding assays, or does INDIGO offer binding assays?

INDIGO’s assays are not binding assays. They are cell-based trans-activation assays, and the principal application is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that the compounds may exert against the nuclear receptors. INDIGO reporter systems utilize firefly luciferase reporter gene technology, and while there is a binding taking place, our assays do not measure it. Instead, the luciferase light response is measured which correlates to the activation status of the receptor (either activation or inhibition). Quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in receptor activity. It will tell the scientist the significance (strength) of the interaction by the level of light emitted. In addition, cell-based assays are more sensitive and able to detect smaller levels of activation.

What cell lines/types are used?

INDIGO’s nuclear receptor assays utilize proprietary human and non-human mammalian cells engineered to provide constitutive, high-level expression of the designated receptor. Specific cell type information for each assay is proprietary and available only through consultation with INDIGO’s technical team following a screening service or assay kit purchase.

Do INDIGO’s nuclear receptor assays express the native, full-length receptor?

Reporter cells included in INIDIGO’s steroid hormone nuclear assay kits (ERα, ERβ, AR, PGR, MR, GR) express the native, full-length receptors. INDIGO’s other nuclear receptor assays, however, include reporter cells that express hybrid nuclear receptors. In these cases, the respective receptor’s native N-terminal sequence comprising the DNA Binding Domain (DBD) has been replaced with sequence encoding the yeast Gal4-DBD. All other native NR functional/structural domains (ligand binding domain, hinge region, and various activation domains) are present in these hybrid receptors. These reporter cells also contain the firefly luciferase reporter gene functionally linked to the upstream genetic response element for Gal4. Consequently, once a bioactive compound associates with the ligand binding domain of the hybrid receptor, only the luciferase reporter gene is induced. Ligand-activation of the hybrid receptor will not induce collateral expression of target genes that are otherwise regulated by the native nuclear receptor.

Can you tell me what the reference agonist/antagonist is for your receptor assays?

View our list of reference compounds. We do utilize commercially available reference agonists for our assays, and this reference agonist is included in each assay kit. We do use reference antagonists where a reference is known and commercially available. Some of our antagonist assays do not have reference antagonists available.

Do I need any special equipment to utilize INDIGO’s assay kits?

Each kit includes luciferase reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), and a detailed Technical Manual. A luminometer is required for Relative Light Unit (RLU) measurements.

Can INDIGO’s Nuclear Receptor Assay Kits be used for diagnostic purposes?

INDIGO’s kits are intended for research purposes only, and not for diagnostic or therapeutic use in humans.

Transcription Factor Assay Kits: Unveil the Possibilities

Unveil the power of our cell-based luciferase reporter Transcription Factor Assay Kits for your research.

  • Enable in-depth investigations into cellular processes and signaling pathways
  • Gain insights into gene regulation mechanisms by measuring transcription factor activity in live cells
Description Sizes Catalog # Species Target Type Associated Disease
Human AP-1 Reporter Assay Kit 1 x 96-well format assays IB24001 Human Transcription Factor Cancer
Human AP-1 Reporter Assay Kit 3 x 32 assays in 96-well format IB24001-32 Human Transcription Factor Cancer
Human NF-kB Reporter Assay Kit 1 x 96-well format assays IB09001 Human Transcription Factor Autoimmune, Cancer, Cardiovascular, Dermatitis, Kidney Disease, Neurodegenerative
Human NF-kB Reporter Assay Kit 3 x 32 assays in 96-well format IB09001-32 Human Transcription Factor Autoimmune, Cancer, Cardiovascular, Dermatitis, Kidney Disease, Neurodegenerative
Human NF-kB Reporter Assay Kit 1 x 384-well format assays IB09002 Human Transcription Factor Autoimmune, Cancer, Cardiovascular, Dermatitis, Kidney Disease, Neurodegenerative
Human p53 Assay Kit 2x 48 assays in 96-well assay plates IB25001-48 Human Transcription Factor Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Human TEAD4/YAP Assay Kit 1 x 96-well format assays IB16001 Human Transcription Factor Cancer, Cardiovascular, Kidney Disease, Neurodegenerative, Obesity, Reproduction
Human TEAD4/YAP Assay Kit 3 x 32 assays in 96-well format IB16001-32 Human Transcription Factor Cancer, Cardiovascular, Kidney Disease, Neurodegenerative, Obesity, Reproduction
Human TEAD4/YAP Assay Kit 1 x 384-well format assays IB16002 Human Transcription Factor Cancer, Cardiovascular, Kidney Disease, Neurodegenerative, Obesity, Reproduction
NFAT Reporter Assay Kit 1 x 96-well format assays IB18001 Human Transcription Factor Cancer
NFAT Reporter Assay Kit 3 x 32 assays in 96-well format IB18001-32 Human Transcription Factor Cancer
NFAT Reporter Assay Kit 1 x 384-well format assays IB18002 Human Transcription Factor Cancer

Transcription Factors: Regulating Gene Expression and Cellular Responses

Transcription factors are important proteins that play a crucial role in regulating gene expression by binding to specific DNA sequences. These receptors are often found within the cell’s nucleus and act as sensors, detecting signals from the external environment or cellular processes.

Upon signal activation, transcription factors undergo conformational change, bind to DNA, then directly influence gene transcription. By binding to DNA sequences known as response elements, transcription factors can either activate or repress the expression of target genes, and thereby modulate various cellular processes and responses.

Transcription factors are involved in a wide range of biological functions, such as embryonic development, immune response, and hormone signaling. Their ability to integrate external signals and initiate gene expression programs makes them key regulators of cellular behavior and adaptation.

Understanding the mechanisms and functions of transcription factors provides insight into how cells perceive and respond to their environment, thus highlighting their significance in various physiological and pathological contexts.

Frequently Asked Questions

Are INDIGO assays binding assays, or does INDIGO offer binding assays?

INDIGO’s assays are not binding assays. They are cell-based trans-activation assays, and the principal application is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that the compounds may exert against the nuclear receptors. INDIGO reporter systems utilize firefly luciferase reporter gene technology, and while there is a binding taking place, our assays do not measure it. Instead, the luciferase light response is measured which correlates to the activation status of the receptor (either activation or inhibition). Quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in receptor activity. It will tell the scientist the significance (strength) of the interaction by the level of light emitted. In addition, cell-based assays are more sensitive and able to detect smaller levels of activation.

What cell lines/types are used?

INDIGO’s nuclear receptor assays utilize proprietary human and non-human mammalian cells engineered to provide constitutive, high-level expression of the designated receptor. Specific cell type information for each assay is proprietary and available only through consultation with INDIGO’s technical team following a screening service or assay kit purchase.

Do INDIGO’s nuclear receptor assays express the native, full-length receptor?

Reporter cells included in INIDIGO’s steroid hormone nuclear assay kits (ERα, ERβ, AR, PGR, MR, GR) express the native, full-length receptors. INDIGO’s other nuclear receptor assays, however, include reporter cells that express hybrid nuclear receptors. In these cases, the respective receptor’s native N-terminal sequence comprising the DNA Binding Domain (DBD) has been replaced with sequence encoding the yeast Gal4-DBD. All other native NR functional/structural domains (ligand binding domain, hinge region, and various activation domains) are present in these hybrid receptors. These reporter cells also contain the firefly luciferase reporter gene functionally linked to the upstream genetic response element for Gal4. Consequently, once a bioactive compound associates with the ligand binding domain of the hybrid receptor, only the luciferase reporter gene is induced. Ligand-activation of the hybrid receptor will not induce collateral expression of target genes that are otherwise regulated by the native nuclear receptor.

Can you tell me what the reference agonist/antagonist is for your receptor assays?

View our list of reference compounds. We do utilize commercially available reference agonists for our assays, and this reference agonist is included in each assay kit. We do use reference antagonists where a reference is known and commercially available. Some of our antagonist assays do not have reference antagonists available.

Do I need any special equipment to utilize INDIGO’s assay kits?

Each kit includes luciferase reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), and a detailed Technical Manual. A luminometer is required for Relative Light Unit (RLU) measurements.

Can INDIGO’s Nuclear Receptor Assay Kits be used for diagnostic purposes?

INDIGO’s kits are intended for research purposes only, and not for diagnostic or therapeutic use in humans.

Cytokine Receptor Assay Kits: Reliable Insights from Robust Assays

Unveil insights into cellular signaling and immune system function with Cytokine Receptor Assay Kits from INDIGO Biosciences.

  • Evaluate amount of receptor activation quickly and easily.
  • Gain insights for research on immune and other disorders.

Quantify agonist or antagonist activity caused by your compounds.

Description Sizes Catalog # Species Target Type Associated Disease
EPOR Reporter Assay Kit 1 x 96-well format assays IB17001 Human Transcription Factor Cancer, Kidney Disease, Neurodegenerative, Reproduction
EPOR Reporter Assay Kit 3 x 32 assays in 96-well format IB17001-32 Human Transcription Factor Cancer, Kidney Disease, Neurodegenerative, Reproduction
EPOR Reporter Assay Kit 1 x 384-well format assays IB17002 Human Transcription Factor Cancer, Kidney Disease, Neurodegenerative, Reproduction
TPOR Reporter Assay Kit 1 x 96-well format assays IB20001 Human Cytokine Receptor Autoimmune, Reproduction
TPOR Reporter Assay Kit 3 x 32 assays in 96-well format IB20001-32 Human Cytokine Receptor Autoimmune, Reproduction
TPOR Reporter Assay Kit 1 x 384-well format assays IB20002 Human Cytokine Receptor Autoimmune, Reproduction

Cytokine Receptors and their Regulation of Cellular Processes

Cytokine receptors are integral components of the immune system and play a crucial role in cell signaling. These receptors are found on the surface of various cells and are activated by cytokines, which are small proteins involved in cell-to-cell communication.

When cytokines bind to their respective receptors on the cell surface, they initiate a cascade of events within the cell. This activation triggers intracellular signaling pathways, which can involve various molecules such as kinases, transcription factors, and secondary messengers. These signaling pathways regulate a wide range of cellular processes, including cell growth, differentiation, proliferation, migration, and apoptosis (cell death).

By modulating these signaling pathways, cytokine receptors help coordinate immune responses, inflammation, and other physiological processes. Dysregulation or malfunctioning of cytokine receptors can lead to immune disorders, autoimmune diseases, and other pathological conditions. Therefore, cytokine receptor assays are crucial for developing targeted therapies and interventions for various diseases.

Frequently Asked Questions

Are INDIGO assays binding assays, or does INDIGO offer binding assays?

INDIGO’s assays are not binding assays. They are cell-based trans-activation assays, and the principal application is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that the compounds may exert against the nuclear receptors. INDIGO reporter systems utilize firefly luciferase reporter gene technology, and while there is a binding taking place, our assays do not measure it. Instead, the luciferase light response is measured which correlates to the activation status of the receptor (either activation or inhibition). Quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in receptor activity. It will tell the scientist the significance (strength) of the interaction by the level of light emitted. In addition, cell-based assays are more sensitive and able to detect smaller levels of activation.

What cell lines/types are used?

INDIGO’s nuclear receptor assays utilize proprietary human and non-human mammalian cells engineered to provide constitutive, high-level expression of the designated receptor. Specific cell type information for each assay is proprietary and available only through consultation with INDIGO’s technical team following a screening service or assay kit purchase.

Do INDIGO’s nuclear receptor assays express the native, full-length receptor?

Reporter cells included in INIDIGO’s steroid hormone nuclear assay kits (ERα, ERβ, AR, PGR, MR, GR) express the native, full-length receptors. INDIGO’s other nuclear receptor assays, however, include reporter cells that express hybrid nuclear receptors. In these cases, the respective receptor’s native N-terminal sequence comprising the DNA Binding Domain (DBD) has been replaced with sequence encoding the yeast Gal4-DBD. All other native NR functional/structural domains (ligand binding domain, hinge region, and various activation domains) are present in these hybrid receptors. These reporter cells also contain the firefly luciferase reporter gene functionally linked to the upstream genetic response element for Gal4. Consequently, once a bioactive compound associates with the ligand binding domain of the hybrid receptor, only the luciferase reporter gene is induced. Ligand-activation of the hybrid receptor will not induce collateral expression of target genes that are otherwise regulated by the native nuclear receptor.

Can you tell me what the reference agonist/antagonist is for your receptor assays?

View our list of reference compounds. We do utilize commercially available reference agonists for our assays, and this reference agonist is included in each assay kit. We do use reference antagonists where a reference is known and commercially available. Some of our antagonist assays do not have reference antagonists available.

Do I need any special equipment to utilize INDIGO’s assay kits?

Each kit includes luciferase reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), and a detailed Technical Manual. A luminometer is required for Relative Light Unit (RLU) measurements.

Can INDIGO’s Nuclear Receptor Assay Kits be used for diagnostic purposes?

INDIGO’s kits are intended for research purposes only, and not for diagnostic or therapeutic use in humans.

Live Cell Multiplex Assay

Sizes Catalog #
1 x 96-well format assays LCM-01
5 x 96-well format assays LCM-05
10 x 96-well format assays LCM-10

INDIGlo Luciferase Detection Reagent Kit

Sizes Catalog #
10 mL each of 2x-concentrated INDIGlo LDR and Dilution Buffer LDR-10
25 mL each of 2x-concentrated INDIGlo LDR and Dilution Buffer LDR-25
50 mL each of 2x-concentrated INDIGlo LDR and Dilution Buffer LDR-50

PRODUCTS BY APPLICATION

Drug Discovery Assay Kits: Comprehensive & Reliable Screening Solutions

Rapidly screen therapeutic candidates today and accelerate drug development decisions for a better tomorrow.

With INDIGO’s Drug Discovery Assay Kits, you can:

  • Quickly identify both on- and off-target effects.
  • Gain insights into drug-drug and drug-nutrient interactions.
  • Screen drug effects on a specific receptor or full panels in agonist or antagonist mode.
  • Easily perform cross-species comparisons with our ortholog kits.
Description Sizes Catalog # Species Target Type Associated Disease
CB1R Reporter Assay Kit 1 x 96-well format assays IB19001 Human GPCR Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis
CB1R Reporter Assay Kit 3 x 32 assays in 96-well format IB19001-32 Human GPCR Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis
CB1R Reporter Assay Kit 1 x 384-well format assays IB19002 Human GPCR Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis
Cyn Monkey FXR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format C00601 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Cyn Monkey PGR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format C05001 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Obesity, Osteoporosis, Reproduction
Cyn Monkey PPARa Reporter Assay Kit 1 x 96-well format assayst / 3 x 32 assays in 96-well format C00111 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Cyn Monkey PPARd Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format C00121 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Cyn Monkey PPARg Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format C00101 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Cyn Monkey PXR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format C07001 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Dog FXR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format D00601 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Dog LXRa Reporter Assay Kit 1 x 96-well format assays D00311 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, NASH/NAFLD, Reproduction
Dog LXRb Reporter Assay Kit 1 x 96-well format assays D00301 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dyslipidemia, Reproduction
Dog PPARa Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format D00111 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Dog PPARd Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format D00121 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Dog PXR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format D07001 Dog Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
EPOR Reporter Assay Kit 1 x 96-well format assays IB17001 Human Cytokine Receptor Cancer, Kidney Disease, Neurodegenerative, Reproduction
EPOR Reporter Assay Kit 3 x 32 assays in 96-well format IB17001-32 Human Cytokine Receptor Cancer, Kidney Disease, Neurodegenerative, Reproduction
EPOR Reporter Assay Kit 1 x 384-well format assays IB17002 Human Cytokine Receptor Cancer, Kidney Disease, Neurodegenerative, Reproduction
Expression Profiling of Clinically Relevant CYPs Assay Kit 2 x 48 assays in 96-well format UGE1003-48 Human
FGFR/β-Klotho Reporter Assay Kit 1 x 96-well format assays IB22001 Human Growth Factor Receptor Cardiovascular, NASH/NAFLD, Obesity, Reproduction, Wound Healing
FGFR/β-Klotho Reporter Assay Kit 3 x 32 assays in 96-well format IB22001-32 Human Growth Factor Receptor Cardiovascular, NASH/NAFLD, Obesity, Reproduction, Wound Healing
FGFR/β-Klotho Reporter Assay Kit 1 x 384-well format assays IB22002 Human Growth Factor Receptor Cardiovascular, NASH/NAFLD, Obesity, Reproduction, Wound Healing
FGFR1/2 Reporter Assay Kit 1 x 96-well format assays IB21001 Human Growth Factor Receptor Autoimmune, Cancer, Kidney Disease, NASH/NAFLD, Neurodegenerative, Reproduction, Wound Healing
FGFR1/2 Reporter Assay Kit 3 x 32 assays in 96-well format IB21001-32 Human Growth Factor Receptor Autoimmune, Cancer, Kidney Disease, NASH/NAFLD, Neurodegenerative, Reproduction, Wound Healing
FGFR1/2 Reporter Assay Kit 1 x 384-well format assays IB21002 Human Growth Factor Receptor Autoimmune, Cancer, Kidney Disease, NASH/NAFLD, Neurodegenerative, Reproduction, Wound Healing
Human AhR Reporter Assay Kit 1 x 96-well format assays IB06001 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Human AhR Reporter Assay Kit 3 x 32 assays in 96-well format IB06001-32 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Human AhR Reporter Assay Kit 1 x 384-well format assays IB06002 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Human AR Reporter Assay Kit 1 x 96-well format assays IB03001 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Human AR Reporter Assay Kit 3 x 32 assays in 96-well format IB03001-32 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Human AR Reporter Assay Kit 1 x 384-well format assays IB03002 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Human CAR-2 Reporter Assay Kit 1 x 96-well format assays IB00921 Human Nuclear Hormone Receptor NASH/NAFLD, Wound Healing
Human CAR-2 Reporter Assay Kit 3 x 32 assays in 96-well format IB00921-32 Human Nuclear Hormone Receptor NASH/NAFLD, Wound Healing
Human CAR-2 Reporter Assay Kit 1 x 384-well format assays IB00922 Human Nuclear Hormone Receptor NASH/NAFLD, Wound Healing
Human CAR-3 Reporter Assay Kit 1 x 96-well format assays IB00901 Human Nuclear Hormone Receptor NASH/NAFLD, Wound Healing
Human CAR-3 Reporter Assay Kit 3 x 32 assays in 96-well format IB00901-32 Human Nuclear Hormone Receptor NASH/NAFLD, Wound Healing
Human CAR-3 Reporter Assay Kit 1 x 384-well format assays IB00902 Human Nuclear Hormone Receptor NASH/NAFLD, Wound Healing
Human EGFR1 Reporter Assay Kit 1 x 96-well format assays IB13001 Human Growth Factor Receptor Cancer, Dermatitis, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Human EGFR1 Reporter Assay Kit 3 x 32 assays in 96-well format IB13001-32 Human Growth Factor Receptor Cancer, Dermatitis, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Human EGFR1 Reporter Assay Kit 1 x 384-well format assays IB13002 Human Growth Factor Receptor Cancer, Dermatitis, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Human ERa Reporter Assay Kit 1 x 96-well format assays IB00401 Human Nuclear Hormone Receptor Autoimmune, Cardiovascular, Dyslipidemia, NASH/NAFLD, Obesity, Osteoporosis, Reproduction, Wound Healing
Human ERa Reporter Assay Kit 3 x 32 assays in 96-well format IB00401-32 Human Nuclear Hormone Receptor Autoimmune, Cardiovascular, Dyslipidemia, NASH/NAFLD, Obesity, Osteoporosis, Reproduction, Wound Healing
Human ERa Reporter Assay Kit 1 x 384-well format assays IB00402 Human Nuclear Hormone Receptor Autoimmune, Cardiovascular, Dyslipidemia, NASH/NAFLD, Obesity, Osteoporosis, Reproduction, Wound Healing
Human ERb Reporter Assay Kit 1 x 96-well format assays IB00411 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Osteoporosis, Reproduction, Wound Healing
Human ERb Reporter Assay Kit 3 x 32 assays in 96-well format IB00411-32 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Osteoporosis, Reproduction, Wound Healing
Human ERb Reporter Assay Kit 1 x 384-well format assays IB00412 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Osteoporosis, Reproduction, Wound Healing
Human ERRa Reporter Assay Kit 1 x 96-well format assays IB08001 Human Nuclear Hormone Receptor Cancer
Human ERRa Reporter Assay Kit 3 x 32 assays in 96-well format IB08001-32 Human Nuclear Hormone Receptor Cancer
Human ERRa Reporter Assay Kit 1 x 384-well format assays IB08002 Human Nuclear Hormone Receptor Cancer
Human ERRb Reporter Assay Kit 1 x 96-well format assays IB08011 Human Nuclear Hormone Receptor Cancer
Human ERRb Reporter Assay Kit 3 x 32 assays in 96-well format IB08011-32 Human Nuclear Hormone Receptor Cancer
Human ERRb Reporter Assay Kit 1 x 384-well format assays IB08012 Human Nuclear Hormone Receptor Cancer
Human ERRg Reporter Assay Kit 1 x 96-well format assays IB08021 Human Nuclear Hormone Receptor Cancer
Human ERRg Reporter Assay Kit 3 x 32 assays in 96-well format IB08021-32 Human Nuclear Hormone Receptor Cancer
Human ERRg Reporter Assay Kit 1 x 384-well format assays IB08022 Human Nuclear Hormone Receptor Cancer
Human FXR Reporter Assay Kit 1 x 96-well format assays IB00601 Human Nuclear Hormone Receptor Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Human FXR Reporter Assay Kit 3 x 32 assays in 96-well format IB00601-32 Human Nuclear Hormone Receptor Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Human FXR Reporter Assay Kit 1 x 384-well format assays IB00602 Human Nuclear Hormone Receptor Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Human GHR Reporter Assay Kit 1 x 96-well format assays IB14001 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human GHR Reporter Assay Kit 3 x 32 assays in 96-well format IB14001-32 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human GHR Reporter Assay Kit 1 x 384-well format assays IB14002 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human GR Reporter Assay Kit 1 x 96-well format assays IB00201 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human GR Reporter Assay Kit 3 x 32 assays in 96-well format IB00201-32 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human GR Reporter Assay Kit 1 x 384-well format assays IB00202 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human IGF-1R Reporter Assay Kit 1 x 96-well format assays IB29001 Human Growth Factor Receptor Cancer, Obesity, Reproduction
Human IGF-1R Reporter Assay Kit 1 x 384-well format assays IB29002 Human Growth Factor Receptor Cancer, Obesity, Reproduction
Human INSRb Reporter Assay Kit 1 x 96-well format assays IB28001 Human Growth Factor Receptor Obesity, Reproduction
Human INSRb Reporter Assay Kit 1 x 384-well format assays IB28002 Human Growth Factor Receptor Obesity, Reproduction
Human LXRa Reporter Assay Kit 1 x 96-well format assays IB00311 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, NASH/NAFLD, Reproduction
Human LXRa Reporter Assay Kit 3 x 32 assays in 96-well format IB00311-32 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, NASH/NAFLD, Reproduction
Human LXRa Reporter Assay Kit 1 x 384-well format assays IB00312 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, NASH/NAFLD, Reproduction
Human LXRb Reporter Assay Kit 1 x 96-well format assays IB00301 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dyslipidemia, Reproduction
Human LXRb Reporter Assay Kit 3 x 32 assays in 96-well format IB00301-32 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dyslipidemia, Reproduction
Human LXRb Reporter Assay Kit 1 x 384-well format assays IB00302 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dyslipidemia, Reproduction
Human MR Reporter Assay Kit 1 x 96-well format assays IB00501 Human Nuclear Hormone Receptor Cardiovascular, Dyslipidemia, Kidney Disease, Osteoporosis
Human MR Reporter Assay Kit 3 x 32 assays in 96-well format IB00501-32 Human Nuclear Hormone Receptor Cardiovascular, Dyslipidemia, Kidney Disease, Osteoporosis
Human MR Reporter Assay Kit 1 x 384-well format assays IB00502 Human Nuclear Hormone Receptor Cardiovascular, Dyslipidemia, Kidney Disease, Osteoporosis
Human P-Glycoprotein / MDR1 Drug Interaction Assay 2 x 48 format HPGP-48 Human
Human PDGFR a/b Reporter Assay Kit 1 x 96-well format assays IB23001 Human Growth Factor Receptor Wound Healing
Human PDGFR a/b Reporter Assay Kit 3 x 32 assays in 96-well format IB23001-32 Human Growth Factor Receptor Wound Healing
Human PDGFR a/b Reporter Assay Kit 1 x 384-well format assays IB23002 Human Growth Factor Receptor Wound Healing
Human PGR Reporter Assay Kit 1 x 96-well format assays IB05001 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Obesity, Osteoporosis, Reproduction
Human PGR Reporter Assay Kit 3 x 32 assays in 96-well format IB05001-32 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Obesity, Osteoporosis, Reproduction
Human PGR Reporter Assay Kit 1 x 384-well format assays IB05002 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Obesity, Osteoporosis, Reproduction
Human PPARa Reporter Assay Kit 1 x 96-well format assays IB00111 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Human PPARa Reporter Assay Kit 3 x 32 assays in 96-well format IB00111-32 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Human PPARa Reporter Assay Kit 1 x 384-well format assays IB00112 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Human PPARd Reporter Assay Kit 1 x 96-well format assays IB00121 Human Nuclear Hormone Receptor Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Human PPARd Reporter Assay Kit 3 x 32 assays in 96-well format IB00121-32 Human Nuclear Hormone Receptor Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Human PPARd Reporter Assay Kit 1 x 384-well format assays IB00122 Human Nuclear Hormone Receptor Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Human PPARg Reporter Assay Kit 1 x 96-well format assays IB00101 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human PPARg Reporter Assay Kit 3 x 32 assays in 96-well format IB00101-32 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human PPARg Reporter Assay Kit 1 x 384-well format assays IB00102 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human PXR Reporter Assay Kit 1 x 96-well format assays IB07001 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Human PXR Reporter Assay Kit 3 x 32 assays in 96-well format IB07001-32 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Human PXR Reporter Assay Kit 1 x 384-well format assays IB07002 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Human RARa Reporter Assay Kit 1 x 96-well format assays IB02201 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RARa Reporter Assay Kit 3 x 32 assays in 96-well format IB02201-32 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RARa Reporter Assay Kit 1 x 384-well format assays IB02202 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RARb Reporter Assay Kit 1 x 96-well format assays IB02101 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RARb Reporter Assay Kit 3 x 32 assays in 96-well format IB02101-32 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RARb Reporter Assay Kit 1 x 384-well format assays IB02102 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RARg Reporter Assay Kit 1 x 96-well format assays IB02001 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RARg Reporter Assay Kit 3 x 32 assays in 96-well format IB02001-32 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RARg Reporter Assay Kit 1 x 384-well format assays IB02002 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RORa Reporter Assay Kit 1 x 96-well format assays IB04011 Human Nuclear Hormone Receptor Cancer, Dyslipidemia, NASH/NAFLD
Human RORa Reporter Assay Kit 3 x 32 assays in 96-well format IB04011-32 Human Nuclear Hormone Receptor Cancer, Dyslipidemia, NASH/NAFLD
Human RORa Reporter Assay Kit 1 x 384-well format assays IB04012 Human Nuclear Hormone Receptor Cancer, Dyslipidemia, NASH/NAFLD
Human RORg Reporter Assay Kit 1 x 96-well format assays IB04001 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, NASH/NAFLD, Obesity
Human RORg Reporter Assay Kit 3 x 32 assays in 96-well format IB04001-32 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, NASH/NAFLD, Obesity
Human RORg Reporter Assay Kit 1 x 384-well format assays IB04002 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, NASH/NAFLD, Obesity
Human RXRa Reporter Assay Kit 1 x 96-well format assays IB00801 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RXRa Reporter Assay Kit 3 x 32 assays in 96-well format IB00801-32 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RXRa Reporter Assay Kit 1 x 384-well format assays IB00802 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RXRb Reporter Assay Kit 1 x 96-well format assays IB00811 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RXRb Reporter Assay Kit 3 x 32 assays in 96-well format IB00811-32 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RXRb Reporter Assay Kit 1 x 384-well format assays IB00812 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Human RXRg Reporter Assay Kit 1 x 96-well format assays IB00821 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Dyslipidemia, Obesity, Wound Healing
Human RXRg Reporter Assay Kit 3 x 32 assays in 96-well format IB00821-32 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Dyslipidemia, Obesity, Wound Healing
Human RXRg Reporter Assay Kit 1 x 384-well format assays IB00822 Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Dyslipidemia, Obesity, Wound Healing
Human TEAD4/YAP Assay Kit 1 x 96-well format assays IB16001 Human Transcription Factor Cancer, Cardiovascular, Kidney Disease, Neurodegenerative, Obesity, Reproduction
Human TEAD4/YAP Assay Kit 3 x 32 assays in 96-well format IB16001-32 Human Transcription Factor Cancer, Cardiovascular, Kidney Disease, Neurodegenerative, Obesity, Reproduction
Human TEAD4/YAP Assay Kit 1 x 384-well format assays IB16002 Human Transcription Factor Cancer, Cardiovascular, Kidney Disease, Neurodegenerative, Obesity, Reproduction
Human TGFbR Reporter Assay Kit 1 x 96-well format assays IB12001 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Wound Healing
Human TGFbR Reporter Assay Kit 3 x 32 assays in 96-well format IB12001-32 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Wound Healing
Human TGFbR Reporter Assay Kit 1 x 384-well format assays IB12002 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Wound Healing
Human TGR5 Reporter Assay Kit 1 x 96-well format assays IB26001 Human GPCR Cardiovascular
Human TGR5 Reporter Assay Kit 1 x 384-well format assays IB26002 Human GPCR Cardiovascular
Human TRa Reporter Assay Kit 1 x 96-well format assays IB01001 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Human TRa Reporter Assay Kit 3 x 32 assays in 96-well format IB01001-32 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Human TRa Reporter Assay Kit 1 x 384-well format assays IB01002 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Human TRb Reporter Assay Kit 1 x 96-well format assays IB01101 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Human TRb Reporter Assay Kit 3 x 32 assays in 96-well format IB01101-32 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Human TRb Reporter Assay Kit 1 x 384-well format assays IB01102 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Human TrkA Reporter Assay Kit 1 x 96-well format assays IB27011 Human Growth Factor Receptor Autoimmune, Cancer, Obesity
Human TrkA Reporter Assay Kit 1 x 384-well format assays IB27012 Human Growth Factor Receptor Autoimmune, Cancer, Obesity
Human TrkB Reporter Assay Kit 1 x 96-well format assays IB27021 Human Growth Factor Receptor Autoimmune, Cancer, Neurodegenerative, Reproduction
Human TrkB Reporter Assay Kit 1 x 384-well format assays IB27022 Human Growth Factor Receptor Autoimmune, Cancer, Neurodegenerative, Reproduction
Human TrkC Reporter Assay Kit 1 x 96-well format assays IB27031 Human Growth Factor Receptor Cancer, Neurodegenerative
Human TrkC Reporter Assay Kit 1 x 384-well format assays IB27032 Human Growth Factor Receptor Cancer, Neurodegenerative
Human VDR Reporter Assay Kit 1 x 96-well format assays IB00701 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, Obesity, Osteoporosis, Reproduction
Human VDR Reporter Assay Kit 3 x 32 assays in 96-well format IB00701-32 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, Obesity, Osteoporosis, Reproduction
Human VDR Reporter Assay Kit 1 x 384-well format assays IB00702 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, Obesity, Osteoporosis, Reproduction
Human VEGFR Reporter Assay Kit 1 x 96-well format assays IB15001 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction
Human VEGFR Reporter Assay Kit 3 x 32 assays in 96-well format IB15001-32 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction
Human VEGFR Reporter Assay Kit 1 x 384-well format assays IB15002 Human Growth Factor Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction
in vitro Screening for Drug-Induced Hepatotoxicity Assay Kit 2 x 48 assays in 96-well format ULC1003-48 Human
Mouse AhR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format M06001 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Mouse CAR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format M00901 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs NASH/NAFLD, Wound Healing
Mouse FXR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format M00601 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Mouse GR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format M00201 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Mouse LXRa Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format M00311 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Mouse LXRa Reporter Assay Kit
Mouse LXRb Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format M00301 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dyslipidemia, Reproduction
Mouse PPARa Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format M00111 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Mouse PPARd Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format M00121 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Mouse PXR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format M07001 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Mouse RORg Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format M04001 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Dermatitis, NASH/NAFLD, Obesity
Mouse/Rat PPARg Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format MR00101 Rodent (Mouse / Rat Shared Ortholog) Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
NFAT Reporter Assay Kit 1 x 96-well format assays IB18001 Human Transcription Factor Cancer
NFAT Reporter Assay Kit 3 x 32 assays in 96-well format IB18001-32 Human Transcription Factor Cancer
NFAT Reporter Assay Kit 1 x 384-well format assays IB18002 Human Transcription Factor Cancer
Panel of Human ER Reporter Assays: ERa & ERb Each in 1 x 48 96-well format IB00421-48P Human Nuclear Hormone Receptor
Panel of Human LXR Reporter Assays: LXRa & LXRb Each in 1 x 48 96-well format IB00321-48P Human Nuclear Hormone Receptor
Panel of Human PPAR Reporter Assays: PPARa, PPARd, & PPARg Each in 1 x 32 96-well format IB00131-32P Human Nuclear Hormone Receptor
Panel of Human RAR Reporter Assays: RARa, RARb & RARg Each in 1 x 32 96-well format IB02301-32P Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Panel of Human RXR Reporter Assays: RXRa, RXRb & RXRg Each in 1 x 32 96-well format IB00831-32P Human Nuclear Hormone Receptor Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Panel of Human TR Reporter Assays: TRa & TRb Each in 1 x 48 96-well format IB01201-48P Human Nuclear Hormone Receptor
Rabbit PGR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format RB05001 Rabbit Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Obesity, Osteoporosis, Reproduction
Rat AhR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format R06001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Rat AR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format R03001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Rat ERa Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format R00401 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cardiovascular, Dyslipidemia, NASH/NAFLD, Obesity, Osteoporosis, Reproduction, Wound Healing
Rat ERb Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format R00411 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Osteoporosis, Reproduction, Wound Healing
Rat FXR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format R00601 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Reproduction
Rat GR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format R00201 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Rat LXRb Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format R00301 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dyslipidemia, Reproduction
Rat LXRα Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format R00311 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, NASH/NAFLD, Reproduction
Rat PGR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format R05001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Obesity, Osteoporosis, Reproduction
Rat PPARa Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format R00111 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Dyslipidemia, NASH/NAFLD, Obesity, Reproduction
Rat PPARd Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format R00121 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Dermatitis, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction, Wound Healing
Rat PXR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format R07001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Rat RORγ Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format R04001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Dermatitis, NASH/NAFLD, Obesity
TPOR Reporter Assay Kit 1 x 96-well format assays IB20001 Human Cytokine Receptor Autoimmune, Reproduction
TPOR Reporter Assay Kit 3 x 32 assays in 96-well format IB20001-32 Human Cytokine Receptor Autoimmune, Reproduction
TPOR Reporter Assay Kit 1 x 384-well format assays IB20002 Human Cytokine Receptor Autoimmune, Reproduction
Zebrafish AhR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format Z06001 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Zebrafish AR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format Z03001 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Zebrafish ERa Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format Z00401 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Osteoporosis, Reproduction, Wound Healing
Zebrafish GR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format Z00201 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Zebrafish PPARg Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format Z00101 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Zebrafish RARa isoform A Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format Z02201 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Autoimmune, Cancer, Dermatitis, Reproduction, Wound Healing
Zebrafish TRb Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format Z01101 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dyslipidemia, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction

Evaluate Drug Candidates’ Safety from Every Angle with INDIGO’s Drug Discovery Assays

Did you know that almost 90% of candidate drugs fail in the first stage of trials?
Your drug lead can reverse the trend!

With INDIGO’s in vitro drug discovery assays, save resources and energy by identifying and characterizing adverse effects and potential risks before your drug candidate progresses further in the drug development process.

Explore INDIGO’s in vitro solutions in the broad categories of Prospective Screens, Safety Pharmacology, and Retrospective Screens, and start focusing your efforts on the best drug leads only.

Prospective Screens

Explore drug-drug interactions with INDIGO’s Nuclear Receptor Profiling and accurately predict the adverse effects of your molecule of interest.

Or dig deeper downstream of nuclear receptor activation through quantification of gene expression using INDIGO’s assay services.

Safety Pharmacology

Evaluate your compounds on major receptors with INDIGO’s reporter assay panels to understand their biological and toxicological effects better.

Identify therapeutic agents with the highest selectivity and lowest potential for off-target and unwanted effects with INDIGO’s cell-based receptor assays.

Retrospective Screens

Unexpected results from clinical trials or animal studies? Trouble-shoot with INDIGO’s large portfolio of ortholog assays to quickly determine if the response observed in laboratory animals is concordant with humans.

Explore Additional INDIGO’s in vitro Solutions to Improve Drug Discovery

Quickly gain insights into the mechanisms underlying the involvement of key receptors in various diseases with INDIGO’s reporter assays.

Understand a compound’s on- and off-target effects and liabilities on overlapping pathways with nuclear receptors functional classifications.

Frequently Asked Questions

Do I need any special equipment to utilize INDIGO’s assay kits?

Each kit includes luciferase reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), and a detailed Technical Manual. A luminometer is required for Relative Light Unit (RLU) measurements.

Do INDIGO's Nuclear Receptor Assay Kits use stable cell lines or transiently transfected cells?

INDIGO’s Nuclear Receptor Assay Kits use transiently transfected cells, less prone to genetic drift and batch-to-batch variations. Moreover, they do not require cell culture, significantly reducing time and contamination risks. Simply thaw the reporter cells, and you are ready to perform the assay.

Can INDIGO’s Nuclear Receptor Assay Kits be used for diagnostic purposes?

INDIGO’s Nuclear Receptor Assay Kits are not for diagnostic purposes.

What is the best way to normalize data (e.g., cell number, protein content)?

We do not recommend normalizing RLU data to cell numbers or protein content. The suspension of reporter cells is a ‘master reagent’ that should be dispensed with high precision into all assay plate wells. Any significant downstream variability in cell number or health will result from cytotoxicity induced by the test compound treatments. To identify and weed out false-positive data, it is essential to perform cytotoxicity analyses for antagonist-mode assays (and inverse-agonist mode assays). INDIGO’s Live Cell Multiplex (LCM) Assay works well for that purpose. However, attempting to normalize RLU data from treated cells in metabolic crisis to untreated, healthy control cells can result in gross misinterpretations of the data when performing cell-based trans-activation assays (which rely on the coordinated cell-cycle processes of gene induction, transcription, translation, mRNA and protein turn-over) “healthy cells” and “dying cells” are not equal units, so attempting to normalize assay data to any cell-related endpoint is not a sound method.

What concentration of organic solvent carried over into the assay wells is acceptable for INDIGO reporter assays?

The concentration of organic solvent carried over into the assay wells should not exceed 0.1%. We recommend no more than 0.4% DMSO carry-over into the assay wells.

Environmental Monitoring Assay Kits

Unleash the power of rapid bioactivity evaluation and toxicity assessment for environmental testing applications.

With INDIGO’s bioassays, in as little as 24 hours, you can:

  • Obtain bioactivity data relative to a standard reference compound.
  • Screen multiple environmental samples simultaneously.
  • Evaluate potential toxicity relative to maximum tolerated level (MTL).
Description Sizes Catalog # Species Target Type Associated Disease
AhR Bioassay for Environmental Sample Monitoring 1 x 96-well format assays EM06001 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Androgen Receptor Bioassay for Environmental Sample Monitoring 1 x 96-well format assays EM03001 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dyslipidemia, Kidney Disease, NASH/NAFLD, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Estrogen Receptor Bioassay for Environmental Sample Monitoring 1 x 96-well format assays EM00431 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Osteoporosis, Reproduction, Wound Healing
Glucocorticoid Receptor Bioassay for Environmental Sample Monitoring 1 x 96-well format assays EM00201 Human Nuclear Hormone Receptor Autoimmune, Cancer, Cardiovascular, Dermatitis, Dyslipidemia, Kidney Disease, NASH/NAFLD, Obesity, Osteoporosis, Reproduction
Human p53 Assay Kit 2x 48 assays in 96-well assay plates IB25001-48 Human Transcription Factor Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Mineralocorticoid Receptor Bioassay for Environmental Sample Monitoring 1 x 96-well format assays EM00501 Human Nuclear Hormone Receptor Cardiovascular, Dyslipidemia, Kidney Disease, Osteoporosis

Protect Health and Ecosystems with INDIGO’s Bioassays for Environmental Testing

INDIGO’s effect-based bioassays for environmental testing help scientists working in environmental toxicology and water quality protect human and environmental health.

Evaluate the biological effects of individual compounds or complex mixtures of contaminants, such as those that may exist in wastewater, recycled water, and surface water. Screen for bioactivity from known contaminants such as estrogen-, androgen-, and dioxin-like compounds. Evaluate activity from unknown substances or complex mixtures of contaminants, such as those that may exist in various environmental matrices.

INDIGO’s environmental testing assays share a standardized workflow, so scientists—even those unfamiliar with cell-based assays—can move easily from one assay to another when evaluating an environmental sample’s effects on multiple receptors. Assay data allows you to assess a sample’s bioactivity compared to an established effect-based trigger value for a given environmental contaminant.

Academic Research

INDIGO’s cell-based assays can help inform your research into environmental contamination and its toxicological impacts on human and ecosystem health.

Regulatory Compliance

INDIGO’s environmental testing assays can help water authorities and industrial companies ensure they are meeting regulatory requirements.

Chemical Development

Evaluate the environmental and toxicological effects of newly developed chemicals for industrial and agricultural applications.

Product Literature

Frequently Asked Questions

Do I need any special equipment to utilize INDIGO’s assay kits?

Each kit includes luciferase reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), and a detailed Technical Manual. A luminometer is required for Relative Light Unit (RLU) measurements.

Do INDIGO's Nuclear Receptor Assay Kits use stable cell lines or transiently transfected cells?

INDIGO’s Nuclear Receptor Assay Kits use transiently transfected cells, less prone to genetic drift and batch-to-batch variations. Moreover, they do not require prolonged cell culture, significantly reducing time and contamination risks. Simply thaw the reporter cells, and you are ready to perform the assay. Contact us to learn how our assay kits can fit into your workflow.

Are INDIGO’s assays approved by any regulatory authorities?

Yes. INDIGO’s Aryl Hydrocarbon Receptor (AhR) and Estrogen Receptor Alpha (ERα) bioassay have been approved by the California Water Boards, State Water Resources Control Board, for use as bioanalytical screening tools for Constituents of Emerging Concern (CECs) in recycled water.

Toxicology Assay Kits

Rapidly assess therapeutic candidates’ potential for drug-drug interactions and accurately predict xenobiotic-induced liver toxicity. INDIGO’s toxicology assay kits provide rapid, reliable results for:

  • Prospective screening
  • Retrospective screening
  • Safety pharmacology
Description Sizes Catalog # Species Target Type Associated Disease
Cyn Monkey PXR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format C07001 Cyn Monkey Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Dog PXR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format D07001 Dog Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Expression Profiling of Clinically Relevant CYPs Assay Kit 2 x 48 assays in 96-well format UGE1003-48 Human
Human AhR Reporter Assay Kit 1 x 96-well format assays IB06001 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Human AhR Reporter Assay Kit 3 x 32 assays in 96-well format IB06001-32 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Human AhR Reporter Assay Kit 1 x 384-well format assays IB06002 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Human AP-1 Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format IB24001 Human Transcription Factor Cancer
Human CAR-2 Reporter Assay Kit 1 x 96-well format assays IB00921 Human Nuclear Hormone Receptor NASH/NAFLD, Wound Healing
Human CAR-2 Reporter Assay Kit 3 x 32 assays in 96-well format IB00921-32 Human Nuclear Hormone Receptor NASH/NAFLD, Wound Healing
Human CAR-2 Reporter Assay Kit 1 x 384-well format assays IB00922 Human Nuclear Hormone Receptor NASH/NAFLD, Wound Healing
Human CAR-3 Reporter Assay Kit 1 x 96-well format assays IB00901 Human Nuclear Hormone Receptor NASH/NAFLD, Wound Healing
Human CAR-3 Reporter Assay Kit 3 x 32 assays in 96-well format IB00901-32 Human Nuclear Hormone Receptor NASH/NAFLD, Wound Healing
Human CAR-3 Reporter Assay Kit 1 x 384-well format assays IB00902 Human Nuclear Hormone Receptor NASH/NAFLD, Wound Healing
Human NF-kB Reporter Assay Kit 1 x 96-well format assays IB09001 Human Transcription Factor Autoimmune, Cancer, Cardiovascular, Dermatitis, Kidney Disease, Neurodegenerative
Human NF-kB Reporter Assay Kit 3 x 32 assays in 96-well format IB09001-32 Human Transcription Factor Autoimmune, Cancer, Cardiovascular, Dermatitis, Kidney Disease, Neurodegenerative
Human NF-kB Reporter Assay Kit 1 x 384-well format assays IB09002 Human Transcription Factor Autoimmune, Cancer, Cardiovascular, Dermatitis, Kidney Disease, Neurodegenerative
Human Nrf2 Reporter Assay Kit 1 x 96-well format assays IB10001 Human Nuclear Hormone Receptor Cancer, Cardiovascular, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction
Human Nrf2 Reporter Assay Kit 3 x 32 assays in 96-well format IB10001-32 Human Nuclear Hormone Receptor Cancer, Cardiovascular, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction
Human Nrf2 Reporter Assay Kit 1 x 384-well format assays IB10002 Human Nuclear Hormone Receptor Cancer, Cardiovascular, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction
Human P-Glycoprotein / MDR1 Drug Interaction Assay 2 x 48 format HPGP-48 Human
Human p53 Assay Kit 2x 48 assays in 96-well assay plates IB25001-48 Human Transcription Factor Autoimmune, Cancer, Cardiovascular, Neurodegenerative, Obesity, Osteoporosis, Reproduction
Human PXR Reporter Assay Kit 1 x 96-well format assays IB07001 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Human PXR Reporter Assay Kit 3 x 32 assays in 96-well format IB07001-32 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Human PXR Reporter Assay Kit 1 x 384-well format assays IB07002 Human Nuclear Hormone Receptor Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
in vitro Screening for Drug-Induced Hepatotoxicity Assay Kit 2 x 48 assays in 96-well format ULC1003-48 Human
Mouse AhR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format M06001 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Mouse CAR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format M00901 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs NASH/NAFLD, Wound Healing
Mouse PXR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format M07001 Mouse Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Rat AhR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format R06001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
Rat PXR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format R07001 Rat Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
Zebrafish AhR Reporter Assay Kit 1 x 96-well format assays / 3 x 32 assays in 96-well format Z06001 Zebrafish Nuclear Hormone Receptor Nuclear Receptor Orthologs Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing

upcycte Hepatocytes in INDIGO Toxicology Kits

Xenobiotic-induced liver injury is a major cause of human morbidity and mortality. A key reason for this problem is our inability to predict hepatotoxicity at the preclinical stage using currently available model systems. Such models include in vivo animal models and in vitro models based on human-derived liver cells or transformed cell systems. Species differences in xenobiotic disposition and mechanisms of cytotoxicity can make whole animal studies unreliable for extrapolation to humans. In addition, whole animal models are costly and of low throughput. Therefore, it is essential to develop in vitro models that are more predictive of hepatotoxicity, particularly those that are based on human or “humanized” component cells.

There are two major limitations to the use of human liver cells or their derivatives. First, there are currently limited sources of fresh human hepatocytes worldwide and, when available, they often suffer from low viability and high batch-to-batch variability. Second, the freezing process used to preserve primary hepatocytes and the transformation process needed to make stable and proliferating cell lines results in changes in cell differentiation, proliferation, and metabolic processes.

INDIGO’s in vitro toxicology platform was developed to meet the increasing demand for more predictive models for liver toxicity due to the fact that hepatotoxicity remains a major reason for drug withdrawal from pharmaceutical development and clinical use. Consequently, the use of hepatocytes for the early identification of drug candidates that induce acute hepatotoxicity provides a powerful predictive tool that can inform drug development decisions.

Application of this platform is to screen chemicals for induction and activity of hepatic drug metabolizing enzymes (DME), which have been linked to Non-Alcoholic Fatty Liver Disease, Steatosis, Cholestasis and other conditions.

The platform now utilizes upcyte® human liver cells to assess chemical and drug-induced toxicity.

Expression Profiling of Clinically Relevant CYPs

Cytochrome p450 (CYP) enzymes are responsible for the Phase I metabolism of most drugs. And, it is noteworthy that the expression of cytochrome p450 (CYP) genes are predominantly regulated by ligand-activated receptors/transcription factors such as pregnane X receptor (PXR, NR1I2), constitutive androstane receptor (CAR, NR1I3), aryl hydrocarbon receptor (AhR), farnesoid x receptor (FXR, NR1H4), glucocorticoid receptor (GR, NR3C1), and to lesser degrees liver X receptors (LXR, NR1C3), vitamin D receptor (VDR, NR1I1), and peroxisome proliferator-activated receptor alpha (PPARα, NR1C1). Consequently, drugs that activate any of these xenobiotic sensing receptors can dramatically change the endogenous levels of CYP expression in the liver, potentially impacting the rate of their own metabolism, as well as the metabolism of all other co-administered drugs. Of particular concern are metabolic outcomes that transform a drug to greater potency, or to an altered bioactivity.

Assessing drug-induced changes in the expression of CYP genes provides a reliable predictive indicator of altered (either heightened or inhibited) metabolic activities leading to drug-drug interactions in vivo. Cytochrome p450 enzymes with the greatest clinical relevance belong to CYP1, 2, and 3 families. It is estimated that they are involved in the metabolism of 70-80% of drugs currently on the market. For example, CYP3A4 is estimated to be involved in ~30% of all prescription drugs.

INDIGO’s assay kit for the Expression Profiling of Clinically Relevant CYPs contains optimized reagents for the culturing and treatment of upcyte® hepatocytes to assess drug-induced changes in seven clinically-relevant CYPs: CYP3A4. CYP1A1, CYP2B6, CYP2C8, CYP2C9, CYP2C19, and CYP2E1.

What is MDR1?

Human P-glycoprotein (P-gp) is also known as MDR1 or ABCB1, the human multidrug resistance protein 1. It is a 170kDa transmembrane glycoprotein that functions as an ATP-dependent efflux transporter present in the human body’s epithelial tissues.

P-gp consists of twelve membrane-spanning domains and two cytosolic nucleotide-binding domains. The transmembrane domains comprise several substrate binding pockets capable of interacting with a broad range of both endogenous and foreign small molecule chemotypes. Xenobiotic substrates of P-gp range from pollutants, such as those encountered through unintended exposure to industrial and agricultural chemicals, to small molecule drugs that are intentionally administered for therapeutic benefit.

P-gp is highly expressed in gastrointestinal epithelium, liver, pancreatic and kidney cells, and capillary endothelial cells that establish the blood-brain barrier. Multidrug resistance research has found MDR1 expressed at high levels on the lines of transformed and tumor cells. In combination with the activities of Cytochrome P450 oxidases, the robust efflux activity of P-gp plays a critical role in limiting the absorption and systemic physiological distribution of xenobiotics and facilitating their ultimate elimination from the body. The MDR1 transporter role is important for drug-drug interaction (DDI) and drug safety assessments.

Services

In Vitro Toxicology Testing Services: Timely, Effective Toxicity Analysis

Rapidly assess therapeutic candidates’ potential for drug-drug interactions and accurately predict xenobiotic-induced liver toxicity with INDIGO’s in vitro toxicology testing Services.

Reporter Assay Services: Fast & Comprehensive Screening Within Your Reach

Easily find the assay or panel that meets your needs with INDIGO’s extensive receptor assay portfolio. Simply send your samples and obtain high-quality study reports and data files in a timely manner.

All our Reporter Assay Services include a positive control reference so you can move forward with your data confidently.

Obtain Clear & Extensive Screening Data Quickly from Our Expert Scientists

A Comprehensive Portfolio to Find Your Answers

Browse and choose from the world’s largest nuclear receptor assay library, including more than 40 ortholog assays.

Reliable, Cost-Effective Data in Record Time

Our cost-effective reporter assay services and in-house expertise allow you to quickly unlock crucial information without sacrificing data quality.

Dedicated Technical Support Team

Speak with the scientist conducting your study as needed, and get a comprehensive review of your results once your service study is complete.

Applications for INDIGO’s Luciferase Reporter Assays

Rapidly evaluate the efficacy and safety profile of a drug candidate with INDIGO’s Reporter Assay Services and streamline the development process to safer, more effective pharmaceutical agents.

Effectively screen environmental samples, with INDIGO’s Reporter Assay Services and actively contribute to building a safer environment.

Quickly gain insights into the mechanisms underlying the involvement of key receptors in various diseases with INDIGO’s Reporter Assay Services.

Transform your vision into clear results today.

Frequently Asked Questions

Are INDIGO assays binding assays, or does INDIGO offer binding assays?

INDIGO’s assays are not binding assays. They are cell-based trans-activation assays, and the principal application is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that the compounds may exert against the nuclear receptors. INDIGO reporter systems utilize firefly luciferase reporter gene technology, and while there is a binding taking place, our assays do not measure it. Instead, the luciferase light response is measured which correlates to the activation status of the receptor (either activation or inhibition).

Quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in receptor activity. It will tell the scientist the significance (strength) of the interaction by the level of light emitted. In addition, cell-based assays are more sensitive and able to detect smaller levels of activation.

What cell lines/types are used?

INDIGO’s nuclear receptor assays utilize proprietary human and non-human mammalian cells engineered to provide constitutive, high-level expression of the designated receptor. Specific cell type information for each assay is proprietary and available only through consultation with INDIGO’s technical team following a screening service or assay kit purchase.

Do INDIGO’s nuclear receptor assays express the native, full-length receptor?

Reporter cells included in INIDIGO’s steroid hormone nuclear assay kits (ERα, ERβ, AR, PGR, MR, GR) express the native, full-length receptors. INDIGO’s other nuclear receptor assays, however, include reporter cells that express hybrid nuclear receptors. In these cases, the respective receptor’s native N-terminal sequence comprising the DNA Binding Domain (DBD) has been replaced with sequence encoding the yeast Gal4-DBD. All other native NR functional/structural domains (ligand binding domain, hinge region, and various activation domains) are present in these hybrid receptors. These reporter cells also contain the firefly luciferase reporter gene functionally linked to the upstream genetic response element for Gal4.

Consequently, once a bioactive compound associates with the ligand binding domain of the hybrid receptor, only the luciferase reporter gene is induced. Ligand-activation of the hybrid receptor will not induce collateral expression of target genes that are otherwise regulated by the native nuclear receptor.

Can you tell me what the reference agonist/antagonist is for your receptor assays?

View our list of reference compounds. We do utilize commercially available reference agonists for our assays, and this reference agonist is included in each assay kit. We do use reference antagonists where a reference is known and commercially available. Some of our antagonist assays do not have reference antagonists available.

What kind of data will I receive from my screening study?

Upon completion of your study, you will receive a detailed study report (PDF format) including all assay methods and validation, the raw data and calculations of your study (Excel format), and graphing files of all data (GraphPad Prism format). In addition, you are always welcome to contact INDIGO’s team for assistance in interpretation of your data.

Can I choose the exact concentrations I want my compounds to be screened at?

Treatment concentrations are prepared at INDIGO using serial dilutions in fixed increments; the starting concentration and increment of dilution is specified by the customer via our Study Work Order sheet that accompanies all screening studies. For accurate determination of EC50 and/or IC50 values, we recommend at minimum spanning a 5,000-fold concentration range over 8 doses. This strategy requires a 3.33-fold or 4-fold increment of serial dilution. The minimum doses recommended for EC50 and/or IC50 value is 7 test concentrations.

Gene Expression Profiling

Gene expression is a widely used approach for characterizing biological perturbations, defining the molecular mechanisms of diseases, and making critical decisions about risks associated with compounds of interest. With INDIGO’s Gene Expression Services, you can reliably investigate compounds’ effects on metabolic activities with validated qPCR primers targeting clinically relevant CYP enzymes.

INDIGO’s qPCR Solutions

Quantitative real-time PCR (qPCR) is the gold standard for gene expression analysis. Our team of experts in nuclear receptor biology and toxicology will provide the know-how in gene selection, primer design, and mRNA accumulation analysis.

INDIGO has experience in gene selection for disease and pathway-specific studies including inflammation-, cancer-, diabetes-, and drug metabolism-related gene expression. INDIGO also offers predesigned panels of optimized primers for nuclear receptor target genes including PXR, CAR, and PPARs.

INDIGO will provide data on mRNA levels relative to a housekeeping gene, statistical analysis, and interpretation in our study report.

Get Reliable Data & Clear Gene Expression Reports from Our Expert Scientists

Affordable Gene Expression Solutions

Reliable and cost-effective, we offer Gene Expression testing services designed to provide the answers you need.

Fast Screening & Data Delivery

Access crucial information on gene expression quickly with INDIGO’s gene expression services.

Detailed Reports & Available Technical Support

Our team delivers high-quality data analysis and reviews, putting INDIGO’s many years of experience at your service.

Transform your vision into clear results today.

Frequently Asked Questions

Are INDIGO assays binding assays, or does INDIGO offer binding assays?

INDIGO’s assays are not binding assays. They are cell-based trans-activation assays, and the principal application is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that the compounds may exert against the nuclear receptors. INDIGO reporter systems utilize firefly luciferase reporter gene technology, and while there is a binding taking place, our assays do not measure it. Instead, the luciferase light response is measured which correlates to the activation status of the receptor (either activation or inhibition).

Quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in receptor activity. It will tell the scientist the significance (strength) of the interaction by the level of light emitted. In addition, cell-based assays are more sensitive and able to detect smaller levels of activation.

What cell lines/types are used?

INDIGO’s nuclear receptor assays utilize proprietary human and non-human mammalian cells engineered to provide constitutive, high-level expression of the designated receptor. Specific cell type information for each assay is proprietary and available only through consultation with INDIGO’s technical team following a screening service or assay kit purchase.

Do INDIGO’s nuclear receptor assays express the native, full-length receptor?

Reporter cells included in INIDIGO’s steroid hormone nuclear assay kits (ERα, ERβ, AR, PGR, MR, GR) express the native, full-length receptors. INDIGO’s other nuclear receptor assays, however, include reporter cells that express hybrid nuclear receptors. In these cases, the respective receptor’s native N-terminal sequence comprising the DNA Binding Domain (DBD) has been replaced with sequence encoding the yeast Gal4-DBD. All other native NR functional/structural domains (ligand binding domain, hinge region, and various activation domains) are present in these hybrid receptors. These reporter cells also contain the firefly luciferase reporter gene functionally linked to the upstream genetic response element for Gal4.

Consequently, once a bioactive compound associates with the ligand binding domain of the hybrid receptor, only the luciferase reporter gene is induced. Ligand-activation of the hybrid receptor will not induce collateral expression of target genes that are otherwise regulated by the native nuclear receptor.

Can you tell me what the reference agonist/antagonist is for your receptor assays?

View our list of reference compounds. We do utilize commercially available reference agonists for our assays, and this reference agonist is included in each assay kit. We do use reference antagonists where a reference is known and commercially available. Some of our antagonist assays do not have reference antagonists available.

What kind of data will I receive from my screening study?

Upon completion of your study, you will receive a detailed study report (PDF format) including all assay methods and validation, the raw data and calculations of your study (Excel format), and graphing files of all data (GraphPad Prism format). In addition, you are always welcome to contact INDIGO’s team for assistance in interpretation of your data.

Can I choose the exact concentrations I want my compounds to be screened at?

Treatment concentrations are prepared at INDIGO using serial dilutions in fixed increments; the starting concentration and increment of dilution is specified by the customer via our Study Work Order sheet that accompanies all screening studies. For accurate determination of EC50 and/or IC50 values, we recommend at minimum spanning a 5,000-fold concentration range over 8 doses. This strategy requires a 3.33-fold or 4-fold increment of serial dilution. The minimum doses recommended for EC50 and/or IC50 value is 7 test concentrations.

In Vitro Toxicology Testing Services: Timely, Effective Toxicity Analysis

Rapidly assess therapeutic candidates’ potential for drug-drug interactions and accurately predict xenobiotic-induced liver toxicity with INDIGO’s in vitro toxicology testing Services.

Get Reliable Data & Clear Toxicity Reports from Our Expert Scientists

Affordable In Vitro Toxicology Solutions

Reliable and cost-effective, we offer in vitro toxicity testing services designed to provide the answers you need.

Fast Screening & Data Delivery

Access crucial toxicology information quickly with INDIGO’s in vitro toxicity testing services.

Detailed Reports & Available Technical Support

Our team delivers high-quality data analysis and reviews, putting INDIGO’s many years of experience at your service.

Human P-Glycoprotein/MDR1 Drug Interaction Assay Services

Get your therapeutic candidates screened early and quickly identify their potential effects on P-gp, also known as MDR1, a key protein involved in cross-membrane drug transport.

Upgrade your safety assessment process with INDIGO’s Human P-Glycoprotein (P-gp) Interaction Assay Services and fast-track your drugs to FDA validation.

Hepatotoxicity Assay using Highly Stable & Functional upcyte® Hepatocytes

INDIGO’s in vitro screening services for drug-induced hepatotoxicity allow for early assessment of drug candidates, lowering risks of clinical trial termination and post-market withdrawal.

Using single-donor upcyte® Hepatocytes, INDIGO’s hepatotoxicity assays enable accurate, large-scale testing even over extended periods.

Engineered with the ability to proliferate while maintaining key features of primary cells, upcyte® Hepatocytes are highly stable, offering unparalleled assay reproducibility.

Applications for INDIGO’s In Vitro Toxicity Testing Assays

Rapidly evaluate the efficacy and safety profile of a drug candidate with INDIGO’s Reporter Assay Services and streamline the development process to safer, more effective pharmaceutical agents.

Effectively screen environmental samples, with INDIGO’s Reporter Assay Services and actively contribute to building a safer environment.

Transform your vision into clear results today.

Frequently Asked Questions

Are INDIGO assays binding assays, or does INDIGO offer binding assays?

INDIGO’s assays are not binding assays. They are cell-based trans-activation assays, and the principal application is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that the compounds may exert against the nuclear receptors. INDIGO reporter systems utilize firefly luciferase reporter gene technology, and while there is a binding taking place, our assays do not measure it. Instead, the luciferase light response is measured which correlates to the activation status of the receptor (either activation or inhibition).

Quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in receptor activity. It will tell the scientist the significance (strength) of the interaction by the level of light emitted. In addition, cell-based assays are more sensitive and able to detect smaller levels of activation.

What cell lines/types are used?

INDIGO’s nuclear receptor assays utilize proprietary human and non-human mammalian cells engineered to provide constitutive, high-level expression of the designated receptor. Specific cell type information for each assay is proprietary and available only through consultation with INDIGO’s technical team following a screening service or assay kit purchase.

Do INDIGO’s nuclear receptor assays express the native, full-length receptor?

Reporter cells included in INIDIGO’s steroid hormone nuclear assay kits (ERα, ERβ, AR, PGR, MR, GR) express the native, full-length receptors. INDIGO’s other nuclear receptor assays, however, include reporter cells that express hybrid nuclear receptors. In these cases, the respective receptor’s native N-terminal sequence comprising the DNA Binding Domain (DBD) has been replaced with sequence encoding the yeast Gal4-DBD. All other native NR functional/structural domains (ligand binding domain, hinge region, and various activation domains) are present in these hybrid receptors. These reporter cells also contain the firefly luciferase reporter gene functionally linked to the upstream genetic response element for Gal4.

Consequently, once a bioactive compound associates with the ligand binding domain of the hybrid receptor, only the luciferase reporter gene is induced. Ligand-activation of the hybrid receptor will not induce collateral expression of target genes that are otherwise regulated by the native nuclear receptor.

Can you tell me what the reference agonist/antagonist is for your receptor assays?

View our list of reference compounds. We do utilize commercially available reference agonists for our assays, and this reference agonist is included in each assay kit. We do use reference antagonists where a reference is known and commercially available. Some of our antagonist assays do not have reference antagonists available.

What kind of data will I receive from my screening study?

Upon completion of your study, you will receive a detailed study report (PDF format) including all assay methods and validation, the raw data and calculations of your study (Excel format), and graphing files of all data (GraphPad Prism format). In addition, you are always welcome to contact INDIGO’s team for assistance in interpretation of your data.

Can I choose the exact concentrations I want my compounds to be screened at?

Treatment concentrations are prepared at INDIGO using serial dilutions in fixed increments; the starting concentration and increment of dilution is specified by the customer via our Study Work Order sheet that accompanies all screening studies. For accurate determination of EC50 and/or IC50 values, we recommend at minimum spanning a 5,000-fold concentration range over 8 doses. This strategy requires a 3.33-fold or 4-fold increment of serial dilution. The minimum doses recommended for EC50 and/or IC50 value is 7 test concentrations.

Luminescent Cell Viability Testing

Employ INDIGO Biosciences’ Luminescent Cell Viability Testing Services for a powerful tool in assessing cell health and facilitating effective drug discovery and toxicity studies.

Validate Your Antagonist-Mode Data

The LCM assay allows users to validate their primary reporter assay data by determining if their test compound treatments exert mitogenic, cytostatic or cytotoxic activities on the reporter cells. The occurrence of such adverse non-specific effects will always undermine the accurate assessment of a test compound’s potency and/or efficacy as a modulator of receptor function.

When screening test compounds for antagonist activities (or inverse-agonist) it is particularly important to quantify changes in the relative number of live reporter cells at the assay endpoint. Test compounds that exert cytostatic, cytotoxic, or cytolytic activities invariably generate “false-positive” results in an antagonist screen. In such cases, the observed drop in luciferase activity will be incorrectly attributed to inhibition of the nuclear receptor by the test compound. In reality, however, the treatment compound has pushed the reporter cells into division arrest, apoptosis, necrosis, or lysis.

It is important to run this assay in multiplex when designing a study that looks at antagonist or inverse-agonist activities.

Transform your vision into clear results today.

Frequently Asked Questions

Are INDIGO assays binding assays, or does INDIGO offer binding assays?

INDIGO’s assays are not binding assays. They are cell-based trans-activation assays, and the principal application is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that the compounds may exert against the nuclear receptors. INDIGO reporter systems utilize firefly luciferase reporter gene technology, and while there is a binding taking place, our assays do not measure it. Instead, the luciferase light response is measured which correlates to the activation status of the receptor (either activation or inhibition).

Quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in receptor activity. It will tell the scientist the significance (strength) of the interaction by the level of light emitted. In addition, cell-based assays are more sensitive and able to detect smaller levels of activation.

What cell lines/types are used?

INDIGO’s nuclear receptor assays utilize proprietary human and non-human mammalian cells engineered to provide constitutive, high-level expression of the designated receptor. Specific cell type information for each assay is proprietary and available only through consultation with INDIGO’s technical team following a screening service or assay kit purchase.

Do INDIGO’s nuclear receptor assays express the native, full-length receptor?

Reporter cells included in INIDIGO’s steroid hormone nuclear assay kits (ERα, ERβ, AR, PGR, MR, GR) express the native, full-length receptors. INDIGO’s other nuclear receptor assays, however, include reporter cells that express hybrid nuclear receptors. In these cases, the respective receptor’s native N-terminal sequence comprising the DNA Binding Domain (DBD) has been replaced with sequence encoding the yeast Gal4-DBD. All other native NR functional/structural domains (ligand binding domain, hinge region, and various activation domains) are present in these hybrid receptors. These reporter cells also contain the firefly luciferase reporter gene functionally linked to the upstream genetic response element for Gal4.

Consequently, once a bioactive compound associates with the ligand binding domain of the hybrid receptor, only the luciferase reporter gene is induced. Ligand-activation of the hybrid receptor will not induce collateral expression of target genes that are otherwise regulated by the native nuclear receptor.

Can you tell me what the reference agonist/antagonist is for your receptor assays?

View our list of reference compounds. We do utilize commercially available reference agonists for our assays, and this reference agonist is included in each assay kit. We do use reference antagonists where a reference is known and commercially available. Some of our antagonist assays do not have reference antagonists available.

What kind of data will I receive from my screening study?

Upon completion of your study, you will receive a detailed study report (PDF format) including all assay methods and validation, the raw data and calculations of your study (Excel format), and graphing files of all data (GraphPad Prism format). In addition, you are always welcome to contact INDIGO’s team for assistance in interpretation of your data.

Can I choose the exact concentrations I want my compounds to be screened at?

Treatment concentrations are prepared at INDIGO using serial dilutions in fixed increments; the starting concentration and increment of dilution is specified by the customer via our Study Work Order sheet that accompanies all screening studies. For accurate determination of EC50 and/or IC50 values, we recommend at minimum spanning a 5,000-fold concentration range over 8 doses. This strategy requires a 3.33-fold or 4-fold increment of serial dilution. The minimum doses recommended for EC50 and/or IC50 value is 7 test concentrations.

Custom Assay Development

Don’t see the exact receptor you are looking for? Looking to assay a receptor, but in a specific cell line? Contact our team of specialists and design a custom assay tailored to your needs.

Examples of Custom Assay Development Services Available

Custom Receptor Assay

Interested in a human receptor that is not in our current portfolio? We can develop and optimize an assay kit to be utilized in your laboratory or conducted as a service in ours.

Ortholog Receptor Assay

Don’t see the receptor you need in a particular animal model to contrast human receptor activity? Our services lab can clone and optimize an assay for your research.

Receptor Assay in a Specific Cell Line

INDIGO’s team has optimized its reporter assays in a particular cell line, which vary depending on the receptor. If this cell line is not where you need your information, let INDIGO’s staff know and we can develop and optimize the appropriate conditions.

Applications for INDIGO’s Luciferase Reporter Assays

Rapidly evaluate the efficacy and safety profile of a drug candidate with INDIGO’s Reporter Assay Services and streamline the development process to safer, more effective pharmaceutical agents.

Effectively screen environmental samples, with INDIGO’s Reporter Assay Services and actively contribute to building a safer environment.

Quickly gain insights into the mechanisms underlying the involvement of key receptors in various diseases with INDIGO’s Reporter Assay Services.

Transform your vision into clear results today.

Frequently Asked Questions

Are INDIGO assays binding assays, or does INDIGO offer binding assays?

INDIGO’s assays are not binding assays. They are cell-based trans-activation assays, and the principal application is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that the compounds may exert against the nuclear receptors. INDIGO reporter systems utilize firefly luciferase reporter gene technology, and while there is a binding taking place, our assays do not measure it. Instead, the luciferase light response is measured which correlates to the activation status of the receptor (either activation or inhibition).

Quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in receptor activity. It will tell the scientist the significance (strength) of the interaction by the level of light emitted. In addition, cell-based assays are more sensitive and able to detect smaller levels of activation.

What cell lines/types are used?

INDIGO’s nuclear receptor assays utilize proprietary human and non-human mammalian cells engineered to provide constitutive, high-level expression of the designated receptor. Specific cell type information for each assay is proprietary and available only through consultation with INDIGO’s technical team following a screening service or assay kit purchase.

Do INDIGO’s nuclear receptor assays express the native, full-length receptor?

Reporter cells included in INIDIGO’s steroid hormone nuclear assay kits (ERα, ERβ, AR, PGR, MR, GR) express the native, full-length receptors. INDIGO’s other nuclear receptor assays, however, include reporter cells that express hybrid nuclear receptors. In these cases, the respective receptor’s native N-terminal sequence comprising the DNA Binding Domain (DBD) has been replaced with sequence encoding the yeast Gal4-DBD. All other native NR functional/structural domains (ligand binding domain, hinge region, and various activation domains) are present in these hybrid receptors. These reporter cells also contain the firefly luciferase reporter gene functionally linked to the upstream genetic response element for Gal4.

Consequently, once a bioactive compound associates with the ligand binding domain of the hybrid receptor, only the luciferase reporter gene is induced. Ligand-activation of the hybrid receptor will not induce collateral expression of target genes that are otherwise regulated by the native nuclear receptor.

Can you tell me what the reference agonist/antagonist is for your receptor assays?

View our list of reference compounds. We do utilize commercially available reference agonists for our assays, and this reference agonist is included in each assay kit. We do use reference antagonists where a reference is known and commercially available. Some of our antagonist assays do not have reference antagonists available.

What kind of data will I receive from my screening study?

Upon completion of your study, you will receive a detailed study report (PDF format) including all assay methods and validation, the raw data and calculations of your study (Excel format), and graphing files of all data (GraphPad Prism format). In addition, you are always welcome to contact INDIGO’s team for assistance in interpretation of your data.

Can I choose the exact concentrations I want my compounds to be screened at?

Treatment concentrations are prepared at INDIGO using serial dilutions in fixed increments; the starting concentration and increment of dilution is specified by the customer via our Study Work Order sheet that accompanies all screening studies. For accurate determination of EC50 and/or IC50 values, we recommend at minimum spanning a 5,000-fold concentration range over 8 doses. This strategy requires a 3.33-fold or 4-fold increment of serial dilution. The minimum doses recommended for EC50 and/or IC50 value is 7 test concentrations.

Product Literature

Webinars

Technical Drawings

The following drawings make good additions to seminars and classes or just use them as a quick learning tool. Use freely, but please acknowledge the NR Resource. Any addition or corrections, please contact the NRR

NR Nomenclature

NR phylogeny

Structure of NRs

DNA Binding domain

Basic gene expression by NRs

Transcriptional regulation by NRs

NR Ligands

Agonist versus antagonist

NRs and metabolism summary

NRs and metabolism Phase 1

NRs and metabolism Phase 2

NRs and metabolism Phase 3

Mechanisms of endocrine disruption

Mechanism of endocrine disruption

Mechanism of metabolic desruption

NRs and cellular metabolites

NRs and dietary bioactives

Hepatotoxicity IBIPlot

NR expression by function

NR expression by GI and Metabolism

Reverse Cholesterol Transport and NRs

Types of NR Ligands

SHRs Summary

SHR Mechanism of Action

SHR Structure

Conformational Change

Types of ligands (static)

AhR Pathway

AR Pathway

CAR Pathway

FXR Pathway

LRH-1 Pathway

LXR Pathway

PPARα Pathway

PPARβ/δ Pathway

RXRα Pathway

PPARɣ Pathway

PXR Pathway

Retinoic Acid Receptor Alpha (RARα)

RORα Receptor

FAQs

Do I need any special equipment to utilize INDIGO’s assay kits?

The equipment and supplies needed to utilize INDIGO’s assay kits are
standard in most labs. Specifically, the materials you will need to
provide include:

 

Day 1

  • Cell culture-rated laminar flow hood
  • 37°C, humidified 5% CO2 incubator for mammalian cell culture
  • 37°C water bath
  • 70% alcohol wipes
  • 8- or 12-channel electronic, repeat-dispensing pipettes and sterile
    tips
  • Disposable media basins, sterile
  • Sterile multi-channel media basins (such as the Heathrow Scientific “Dual-Function Solution Basin”), or deep-well plates, or appropriate similar vessel for generating dilution series of reference compound(s) and test compound(s)
  • Optional: antagonist reference compound
  • Optional: clear 96-well assay plate, sterile, cell culture treated,
    for viewing cells on Day 2

Day 2

  • Plate-reading luminometer

Can INDIGO’s Nuclear Receptor Assay Kits be used for diagnostic purposes?

INDIGO’s kits are intended for research purposes only, and not for diagnostic or therapeutic use in humans.

What is the difference between the 1×96-well and 3×32 assays in a 96-well plate formats?

INDIGO’s 1×96-well assay kits contain materials to perform assays in a single 96-well assay plate. Our 3×32-well assay kits contain materials to perform three distinct groups of assays in a 96-well plate format. Reagents are configured so that each group will comprise 32 assays. If desired, however, reagents may be combined to perform either 64 or 96 assays. Please note, the aliquot of Reporter Cells is provided as a single-use reagent. Once thawed, reporter cells can NOT be refrozen or maintained in extended culture with any hope of retaining downstream assay performance. Therefore, extra volumes of these reagents should be discarded after assay setup.

Do you have a specific value for positive nuclear receptor activations for your assay kits?

2-fold is what we have found to be historically, mathematically significant.

Do you have a specific value for cell viability that provides reliable nuclear receptor data?

A 15% drop in live cells is considered statistically significant.

Do INDIGO kits use Fetal Bovine Serum?

The compound screening medium (CSM) included in nearly all INDIGO kits contains 10% charcoal-stripped fetal bovine serum (FBS). (The exceptions to this are our RORg assays(5%) and TGF-beta (2%).) If your test substance is human serum, we do recommend serum-free medium, which is available at no charge but must be requested at the time of order.

Which wavelength should I use to read my plate?

INDIGO’s assays are luminescence-based, not fluorescence-based. Therefore, one does not designate specific wavelengths (or filters of any kind) when reading in luminescence mode. Total photon emission is quantified.

Is it okay to plate the test compounds into the wells then immediately add the cells after they have been properly reconstituted?

You always want to add the compounds to the cells, rather than adding the cells to the compounds, per the assay protocol.

What concentration of organic solvent carried over into the assay wells is acceptable for INDIGO reporter assays?

The concentration of organic solvent carried over into the assay wells should not exceed 0.1%. We recommend no more than 0.4% DMSO carry-over into the assay wells.

Can compound screening medium (CSM) and cell recovery medium (CRM) be reused after the initial thaw? (3×32-well assay kit-specific)

It is okay to thaw and keep the CSM/CRM refrigerated between uses. However, we recommend it only be stored at 4°C for a week or less, and that for any time longer than that, that it be refrozen.

Can I thaw and refreeze the 4mM Staurosporine provided in your LCMA kits?

Yes, we recommend keeping it at -20°C.

Can I thaw and refreeze LCMA buffer and LCMA substrate?

Yes, you can thaw the LCMA buffer and keep it at 4°C. You can freeze/thaw the LCMA substrate up to three times.

Can Tryptan Blue be used to check viability (QA) before running the assays?

We do not recommend Tryptan Blue for checking cell viability, as it can be inaccurate for recently thawed cells that are recovering from the trauma of thawing.

How are your products shipped?

All of INDIGO’s assay kits are shipped on dry ice.
Upon receipt, individual kit components may be stored at the temperatures indicated on their respective labels. Alternatively, the entire kit may be further stored at -80°C. To ensure maximal viability, Reporter Cells must be maintained at -80°C until immediately prior to use. The date of product expiration is printed on the Product Qualification Insert (PQI) enclosed with each kit.

What kind of data will I receive from my screening study?

Upon completion of your study, you will receive a detailed study report (PDF format) including all assay methods and validation, the raw data and calculations of your study (Excel format), and graphing files of all data (GraphPad Prism format). In addition, you are always welcome to contact INDIGO’s team for assistance in interpretation of your data.

How long does INDIGO retain client compounds once a study has been completed?

INDIGO retains compounds for three months after reporting results, then disposes of or returns the compounds. If you want us to return your compounds or to store them for more than three months, you must contact us to make appropriate arrangements prior to the three-month window expiring. We reserve the right to charge a disposal fee. We may charge a monthly fee for long-term storage. We will store all compounds under locked, temperature-controlled conditions. An internal chain of custody is maintained for each lot of compound received. Additional information relating to product and service agreements can be found in our Terms and Conditions.

How much of my compound should I send for a screening study?

If test samples are provided as concentrated stock solution, we prefer a 1000x concentration stock relative to the highest treatment concentration (e.g., provide > 10mM stocks if the highest assay concentration is 10 uM). This ensures that the concentration of organic solvent carried over into the assay wells is ~0.1%. INDIGO will not perform studies that require greater than 0.4% DMSO carry-over into the assay wells. You will also need to disclose the solvent used to generate the Master Stock (DMSO preferred—please inquire about the use of alternative organic solvents). Please provide approximately 50uL of 1000x concentrated stock solutions. If test samples are provided in powder form, they must be pre-weighed by the client. INDIGO does not perform manual weighing of test materials. Please provide no more than 5 mg of dry material. You will also need to provide the molecular weight (MW; g/mol) of each test compound. If test samples are provided as neat liquids, provide the Molar concentration of the liquid or provide a pre-weighed volume of the liquid sample (e.g., x mg/100ul). You will also need to provide the molecular weight (MW; g/mol) of each test compound. Aqueous solutions may be submitted, but they must be filter-sterilized and devoid of preservatives and chelating agents. Additional charges will be applied if extensive sample handling is required to generate “assay-ready” stocks.

Why do you recommend running the LCMA for antagonist and inverse-agonist modes, but not agonist mode?

While you are certainly welcome to run the LCMA with agonist mode, we do not typically recommend needing to do so. The reason for this is because cytotoxicity would produce a downward curve. In agonist mode, an agonist response creates an upwards curve and if there are any cytotoxic effects they typically occur towards the top of the curve at the highest doses. Therefore, you would see the downward turn at those levels. In antagonist or inverse-agonist mode, the curve is downward to begin with. In this case, the LCMA is recommended to confirm if the downward curve is a true response or due to cytotoxic effects of the treatment compound.

How many replicates do you recommend?

You can design your study to include as many replicates as you would like. If you are looking to utilize your data to publish, at least three replicates are recommended.

Can I choose the exact concentrations I want my compounds to be screened at?

Treatment concentrations are prepared at INDIGO using serial dilutions in fixed increments; the starting concentration and increment of dilution is specified by the customer via our Study Work Order sheet that accompanies all screening studies. For accurate determination of EC50 and/or IC50 values, we recommend at minimum spanning a 5,000-fold concentration range over 8 doses. This strategy requires a 3.33-fold or 4-fold increment of serial dilution. The minimum doses recommended for EC50 and/or IC50 value is 7 test concentrations.

How many dose concentrations do you recommend?

If you are looking for an initial go/no-go response, we recommend 1-3 dose concentrations. For EC50/IC50 values, we recommend at least 7-8, with 7 being the absolute minimum. You are welcome to do more dose concentrations if preferred, and we find that many of our customers opt to do 10 doses for a full EC50/IC50 curve.

Why don’t INDIGO’s ERα and ERβ assays produce the same EC50 values for 17β-estradiol? Likewise, for your TRα and TRβ assays run against triiodothyronine? There are reports that the respective EC50 values should be the same for these related pairs of nuclear receptors.

First, it is important to understand that EC50 (and IC50) values are not constants. There are numerous variables that impact these, and other, assay metrics. Consequently, these values can vary greatly between different assay types and between different related receptors. Regarding this last point: Neither ERα and ERβ, or TRα and TRβ, are simple iso-forms of each other; rather, they are all distinct receptors encoded by separate genes. As an example, the two ER proteins share only 45% sequence identity. If one includes ‘conserved’ substitutions between the two amino acid sequences, the two receptors still display only 60% sequence homology. While the two ERs have evolved to bind the physiological activator 17β-estradiol, it would be quite surprising if these two different nuclear receptors had the same activation profiles within the cellular context. It is also worth noting that the two ERs display strikingly disparate ligand preferences and activation/inhibition profiles for many other bioactive chemicals. INDIGO’s nuclear receptor assays are cell-based trans-activation assays, which rely on a complex orchestration of the many molecular processes that occur in vivo. In brief, these include: the reporter cells take up exogenous drug, which also experiences competitive interactions with serum-based and cellular macromolecules > binding of the drug to the ligand-binding domain of the nuclear receptor, which initiates the shedding of co-repressor molecules and recruitment of co-activator molecules > nuclear-translocation > binding of the activated receptor complex to the genetic response elements of the gene promoter > formation of a complete transcription complex > induced expression of the target gene (in our case, Luciferase) > translation of mRNA. The mechanics of such cell-based trans-activation assays are in
sharp contrast to in vitro ligand-binding assays and/or co-factor recruitment assays. Such assays measure a simple A+B → AB interaction between two purified molecules, typically polypeptides corresponding to the (much more highly conserved) ligand-binding domain sequences of the respective receptors, or to very small polypeptides corresponding to a specific protein-protein interaction sequence of a co-activator protein.
While useful for some applications, these various in vitro assay formats do not represent the complex molecular interactions that occur between ligands and the native receptors within the cellular environment. Therefore, when comparing the data sets from all of these various assay types, one can expect to see widely varying assay metrics, including respective EC50/IC50 values.

Can INDIGO’s nuclear receptor assays be used to test processed extracts derived from waste-water treatment samples, environmental samples, plant samples, etc.?

Yes. Preferably the extracts will be aqueous preparations that are: 1.) homogenous solutions with no particulate material, and 2.) sterile. If either is not true, the sample should be passed through a 0.2um syringe-tip filter. Preferably aqueous preparations will be no less than 4x-concentrated relative to the final treatment concentration in the assay. If the extract is prepared in DMSO, a stock of no less than 250x-concentrated (preferably more concentrated) is required. Residual DMSO carried over into any of INDIGO’s nuclear receptor assays should never exceed 0.4%. Higher DMSO concentrations will degrade assay performance.

Do INDIGO’s RORγ reporter cells express the RORγt isoform of the receptor?

First, it is important to understand the basic molecular biology behind the RORγ and RORγt isoforms. In humans, RORγ and RORγt derive from the same gene. However, expression of the respective transcripts is driven by tissue-specific alternative promoters, resulting in transcripts with variant exon 1 sequences. Consequently, the translated proteins have unique N-terminal amino acid sequences that encode unique DNA binding domains (DBD) for RORγ and RORγt. Importantly, the RORγ and -γt isoforms have common Ligand Binding Domains, as well as all other functional/structural domains. Therefore, it is their differential expression between tissues and cell types, and their variant DBD sequences, that account for their different physiological activities (target gene expression profiles). INDIGO’s RORγ reporter cells express an engineered cDNA that encodes a hybrid receptor. Specifically, the N-terminal amino acid sequence encoding either the RORγ DBD or the RORγt DBD has been replaced with amino acid sequence encoding the yeast GAL4-DBD. Hence, INDIGO’s reporter cells express a Gal4(DBD)-RORγ hybrid receptor—a “generic RORγ”—that is blind to the isoform differences in function and target gene expression profiles that otherwise exist in vivo. The specific utility of INDIGO’s RORγ assay is to quantify and rank interactions that occur between test compounds and the RORγ LBD. These reporter cells will show RORγ inverse-agonist and agonist responses that may be induced through drug interactions with the receptor.

Can the INDIGO’s ‘inverse-agonist’ receptor assays (RORα, RORγ, ERRα, ERRγ, CAR-1, and LRH-1) be set up to suppress the high constitutive activity of the receptor, thereby allowing the assessment of agonist activities?

Yes. When one is looking for agonist responses exclusively it is a common strategy to suppress the constitutive activity of the receptor by co-treating with a fixed concentration of the inverse-agonist reference compound provided with the assay kit. For example, this strategy is frequently used by researchers screening for agonists of RORγ. RORγ reporter cells are co-treated with a fixed concentration of Ursolic Acid (INDIGO’s reference inverse-agonist) and varying concentrations of the user’s test compounds. Compounds that are putative agonists of RORγ will show a significant dose-dependent increase in RORγ activity above the suppressed level.

What is an inverse-agonist nuclear receptor assay? Are these assays run separately in “agonist” and “inverse-agonist” mode?

Several nuclear receptors express high-level constitutive activity that is (seemingly) independent of a ligand interaction. The RORs, the ERRs, CAR-1, and LRH-1 are nuclear receptors with this activity profile, and they are loosely referred to as “inverse-agonist receptors/assays.” In practice, however, when a bio-active compound associates with the receptor’s ligand binding domain, there will be one of two functional outcomes: 1.) There will be a dose-dependent decrease in the receptor’s constitutive activity (an inverse-agonist response), or 2.) there will be a moderate dose-dependent increase in activity above the receptor’s already high constitutive level (an agonist response). INDIGO’s assays are capable of showing both of these alternative response in a single conventional assay setup.

What is the best way to normalize data (e.g., cell number, protein content)?

Normalizing RLU data to cell numbers or protein content is not something we do or recommend. The suspension of reporter cells is a ‘master reagent’ that should be dispensed with high precision into all wells of the assay plate. Any significant downstream variability in cell number or health will be the result of cytotoxicity induced by the test compound treatments. It is certainly important to perform cytotoxicity analyses for antagonist-mode assays (and inverse-agonist mode assays) to identify and weed out false-positive data, and INDIGO’s Live Cell Multiplex (LCM) Assay works very well for that purpose. However, attempting to normalize RLU data from treated cells that are in metabolic crisis to untreated, healthy control cells can result in gross misinterpretations of the data. When performing cell-based trans-activation assays (which rely on the coordinated cell-cycle processes of gene induction, transcription, translation, mRNA and protein turn-over) “healthy cells” and “dying cells” are not equal units, so attempting to normalize assay data to any cell-related endpoint is not a sound method.

Can you tell me what the reference agonist/antagonist is for your receptor assays?

View our list of reference compounds. We do utilize commercially available reference agonists for our assays, and this reference agonist is included in each assay kit. We do use reference antagonists where a reference is known and commercially available. Some of our antagonist assays do not have reference antagonists available.

Do INDIGO’s nuclear receptor assays express the native, full-length receptor?

Reporter cells included in INIDIGO’s steroid hormone nuclear assay kits (ERα, ERβ, AR, PGR, MR, GR) express the native, full-length receptors. INDIGO’s other nuclear receptor assays, however, include reporter cells that express hybrid nuclear receptors. In these cases, the respective receptor’s native N-terminal sequence comprising the DNA Binding Domain (DBD) has been replaced with sequence encoding the yeast Gal4-DBD. All other native NR functional/structural domains (ligand binding domain, hinge region, and various activation domains) are present in these hybrid receptors. These reporter cells also contain the firefly luciferase reporter gene functionally linked to the upstream genetic response element for Gal4. Consequently, once a bioactive compound associates with the ligand binding domain of the hybrid receptor, only the luciferase reporter gene is induced. Ligand-activation of the hybrid receptor will not induce collateral expression of target genes that are otherwise regulated by the native nuclear receptor.

What cell lines/types are used?

INDIGO’s nuclear receptor assays utilize proprietary human and non-human mammalian cells engineered to provide constitutive, high-level expression of the designated receptor. Specific cell type information for each assay is proprietary and available only through consultation with INDIGO’s technical team following a screening service or assay kit purchase.

Are INDIGO assays binding assays, or does INDIGO offer binding assays?

INDIGO’s assays are not binding assays. They are cell-based trans-activation assays, and the principal application is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that the compounds may exert against the nuclear receptors. INDIGO reporter systems utilize firefly luciferase reporter gene technology, and while there is a binding taking place, our assays do not measure it. Instead, the luciferase light response is measured which correlates to the activation status of the receptor (either activation or inhibition). Quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in receptor activity. It will tell the scientist the significance (strength) of the interaction by the level of light emitted. In addition, cell-based assays are more sensitive and able to detect smaller levels of activation.

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