OUR PRODUCT PLATFORM
INDIGO Biosciences’ receptor assay products are cell-based, luciferase reporter assay systems. They feature engineered receptor-specific reporter cells prepared using our unique CryoMite™ process. Once thawed, reporter cells typically present greater than 95% cell viability and are ready for immediate use. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments. Test compounds may be screened for agonist or antagonist activities against nuclear receptors expressed within the cytoplasm and nuclear environments of healthy, dividing mammalian cells.
Our reporter assays capitalize on the extremely low background, high sensitivity, and broad linear dynamic range of bio-luminescence reporter gene technology. INDIGO’s reporter cells incorporate the cDNA encoding beetle luciferase, a 62 kD protein originating from the North American firefly (Photinus pyralis). Luciferase catalyzes the mono-oxidation of D-luciferin in a Mg+2-dependent reaction that consumes O2 and ATP as co-substrates, and yields as products oxyluciferin, AMP, PPi, CO2, and photon emission. Luminescence intensity of the reaction is quantified using a luminometer and is reported in terms of Relative Light Units (RLU’s). Thus, quantifying changes in luciferase expression in the test sample-treated reporter cells provides a sensitive surrogate measure of changes in activity.
INDIGO’s luciferase reporter cell systems are engineered to provide optimal assay sensitivity and dynamic range when quantifying receptor activity. Following ligand-activation, the receptor complex acts to induce expression of the luciferase reporter. Upon addition of detection reagent, the intensity of light emission from the luciferase reaction directly correlates to the activation status of the specific receptor. All INDIGO reporter assay kits incorporate a detection reagent specially formulated to provide stable light emission between 5 and 90+ minute after initiating the luciferase reaction, thus allowing users the ability to dispense detection reagent into all assay wells prior to commencing activity measurements. This eliminates the need for a luminometer equipped with injectors and allows plates to be processed in batch, which dramatically reduces the start-to-finish read time of assay plates.