Label-free cell isolation from murine splenocytes or other single-cell suspensions

What results can I expect?

Here are some example data for CD3⁺ T cell isolation from murine splenocytes (n = 7) using affinity chromatographic Fab-TACS^®:

High purity: > 91%

High yield: > 85%

High viability: > 91%

CD3⁺ cells were isolated from murine splenocytes (A, n=7) using the affinity chromatographic Fab- TACS^® isolation method. Data are displayed as mean ± SEM. Cells were analyzed by flow cytometry. Representative FACS plots before and after enrichment are shown in (B). An average of 91% CD3⁺ T cells demonstrates that highly pure populations could be isolated. A yield of > 85% (C) also shows that the isolation procedure is very efficient. In addition, a viability of > 91% (D) highlights that this method is stress-free for cells.

What are the advantages of using Fab-TACS^®/Nano-TACS^® for cell isolation?

• The positive cell selection approach permits direct labeling of target cells, which results into highly pure cell populations even from samples with an unknown composition. As all reagents are removed during the isolation procedure, cells are completely label-free, similar to an “untouched” negative cell selection approach.

• The choice between different isolation methods (fluorescent, magnetic, affinity chromatographic) offers the possibility to adapt the isolation procedure to different cell numbers and sample types.

• The affinity chromatographic approach allows the isolation of several samples in parallel due to the easy fitting of columns into standard 15 ml or 50 ml tubes using TACS column adapters.

• This system is highly flexible. All Strep-Tactin^® conjugates can be combined with capture proteins that have a Twin-Strep-tag^®. Therefore, you can use your own antibody in this system. Alternatively, desthiobiotinylated antibodies are an option. If biotinylated antibodies are used, elution with biotin is not possible and therefore we only recommend the use of these antibodies if negative cell selection or cell depletion are required.

What do I need to start?

We offer Fab-TACS^®/Nano-TACS^® Agarose Column Starter Kits that contain all necessary components.

Other mouse spleen cells

Use your own capture proteins (Fab fragments, nanobodies or antibodies) and combine them with our Strep-Tactin^® products for cell isolation!

Requirement: Capture proteins should have a Twin-Strep-tag^®. Desthiobiotinylated or biotinylated (depletion only!) capture proteins are also an option.