Anti-Human Secondary Antibodies

Jackson ImmunoResearch AffiniPure™ Anti-Human secondary antibodies are affinity purified polyclonal antibodies with specificity for human immunoglobulins.

We offer Anti-Human secondary antibodies in a range of host species including Alpaca, Donkey, Goat, Mouse and Rabbit. JIR secondary antibodies are available as whole IgG and F(ab’)2 fragment formats. Fab fragment formats are also available and can be an elegant component of multiple labeling and blocking protocols.

They are available with specificity for whole Ig, Fcγ and F(ab’)2 domains, among other specificities.

Anti-Human Antibodies for Serological Tests

Anti-Human secondary antibodies are an invaluable reagent in the development of high-throughput serological testing kits. JIR Anti-Human antibodies are manufactured to ISO 9001:2015 certification and are available in standard and bulk quantities for worldwide shipment.

JIR Anti-Human secondary antibodies are suitable for the detection of human immunoglobulin isotypes by immunoassay including ELISA, ELISPOT, Rapid test, Enzyme immunoassay (EIA), Luminscent immunoassay (LIA), Fluorescent immunoassay (FIA) and Lateral Flow.

  • Anti-Human IgE
  • Anti-Human IgG
  • Anti-Human IgM
  • Anti-Human IgA

Available with specificities to IgE, IgG, IgM or IgA as well as IgG+IgM and IgG+IgM+IgA. Anti-Human products are highly cross-adsorbed.

Host species options include Alpaca, Donkey, Goat, Mouse and Rabbit.

Biotin, HRP and fluorescent dye conjugates available.

Read More About Serological Testing

Anti-Human Secondary Antibodies are available with the following conjugates:

  • Unconjugated
  • Reporter enzymes (Alkaline Phosphatase (AP) or Horseradish Peroxidase (HRP)
  • Biotinylated (biotin conjugates)
  • Fluorophores (Alexa Fluor®, Brilliant Violet™, Cyanine and protein dye conjugates)
  • Colloidal Gold (Immunogold)
Browse Anti-Human Secondaries
Affinity-purified antibodies are isolated from antisera by immunoaffinity chromatography using target antigens coupled to agarose beads. A proprietary elution process is used to dissociate antibodies from the antigen. Unconjugated affinity-purified antibodies are supplied sterile filtered in phosphate buffer without stabilizers or preservatives. Conjugated affinity-purified antibodies are freeze-dried in phosphate buffer with stabilizers and sodium azide, with the exception of horseradish peroxidase conjugates, which do not contain a preservative.

Cross adsorbed secondary antibodies (min X … Sr Prot)

Secondary antibodies against one species are likely to cross-react with other species unless they have been specifically adsorbed against the other species. Antibodies with “(min X … Sr Prot)” in the description have been tested and/or adsorbed against IgG and/or serum proteins of those species indicated in the parentheses. They are recommended when the presence of immunoglobulins from other species may lead to interfering cross-reactivities.

Find out more about cross-adsorption

Example AffiniPure™ Anti-Human Secondary Antibodies

Description Conjugate Code
Goat Anti-Human IgG (H+L)(min X Bov, Hrs, Ms Sr Prot) Unconjugated 109‑005‑088
HRP 109‑035‑088
Biotin 109‑065‑088
Goat Anti-Human IgG, Fcγ fragment specific Unconjugated 109‑005‑008
HRP 109‑035‑008
Biotin 109‑065‑008
Goat Anti-Human IgG, Fcγ fragment specific(min X Bov, Ms Sr Prot) Unconjugated 109‑005‑190
HRP 109‑035‑190
Biotin 109‑065‑190
Goat Anti-Human IgG, Fcγ fragment specific(min X Bov, Hrs, Ms Sr Prot) Unconjugated 109‑005‑098
HRP 109‑035‑098
Biotin 109‑065‑098
R-PE 109‑115‑098
Goat Anti-Human IgG + IgM (H+L)(min X Bov Sr Prot) Unconjugated 109‑005‑127
HRP 109‑035‑127
Biotin 109‑065‑127
Goat Anti-Human IgM, Fc5μ fragment specific(min X Bov Sr Prot) Unconjugated 109‑005‑129
HRP 109‑035‑129
Biotin 109‑065‑129
F(ab’)2 Fragment Goat Anti-Human IgG, Fcγ fragment specific(min X Bov, Hrs, Ms Sr Prot) Unconjugated 109‑006‑098
HRP 109‑036‑098
Biotin 109‑066‑098
R-PE 109‑116‑098
F(ab’)2 Fragment Goat Anti-Human IgG, Fcγ fragment specific(min X Bov, Ms, Rb Sr Prot) Unconjugated 109‑006‑098
HRP 109‑036‑098
Biotin 109‑066‑098
R-PE 109‑116‑098
Donkey Anti-Human IgG, Fcγ fragment specific(min X Bov, Hrs, Ms Sr Prot) Unconjugated 709‑005‑098
HRP 709‑035‑098
Biotin 709‑065‑098
Donkey Anti-Human IgM, Fc5μ fragment specific(min X Bov, Hrs Sr Prot) Unconjugated 709‑005‑073
HRP 709‑035‑073
Biotin 709‑065‑073

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