Anti-Human Secondary Antibodies

Jackson ImmunoResearch AffiniPure™ Anti-Human secondary antibodies are affinity purified polyclonal antibodies with specificity for human immunoglobulins.

We offer Anti-Human secondary antibodies in a range of host species including Alpaca, Donkey, Goat, Mouse and Rabbit. JIR secondary antibodies are available as whole IgG and F(ab’)2 fragment formats. Fab fragment formats are also available and can be an elegant component of multiple labeling and blocking protocols.

They are available with specificity for whole Ig, Fcγ and F(ab’)2 domains, among other specificities.

Anti-Human Antibodies for Serological Tests

Anti-Human secondary antibodies are an invaluable reagent in the development of high-throughput serological testing kits. JIR Anti-Human antibodies are manufactured to ISO 9001:2015 certification and are available in standard and bulk quantities for worldwide shipment.

JIR Anti-Human secondary antibodies are suitable for the detection of human immunoglobulin isotypes by immunoassay including ELISA, ELISPOT, Rapid test, Enzyme immunoassay (EIA), Luminscent immunoassay (LIA), Fluorescent immunoassay (FIA) and Lateral Flow.

  • Anti-Human IgE
  • Anti-Human IgG
  • Anti-Human IgM
  • Anti-Human IgA

Available with specificities to IgE, IgG, IgM or IgA as well as IgG+IgM and IgG+IgM+IgA. Anti-Human products are highly cross-adsorbed.

Host species options include Alpaca, Donkey, Goat, Mouse and Rabbit.

Biotin, HRP and fluorescent dye conjugates available.

Anti-Human Secondary Antibodies are available with the following conjugates:

  • Unconjugated
  • Reporter enzymes (Alkaline Phosphatase (AP) or Horseradish Peroxidase (HRP)
  • Biotinylated (biotin conjugates)
  • Fluorophores (Alexa Fluor®, Brilliant Violet™, Cyanine and protein dye conjugates)
  • Colloidal Gold (Immunogold)
Affinity-purified antibodies are isolated from antisera by immunoaffinity chromatography using target antigens coupled to agarose beads. A proprietary elution process is used to dissociate antibodies from the antigen. Unconjugated affinity-purified antibodies are supplied sterile filtered in phosphate buffer without stabilizers or preservatives. Conjugated affinity-purified antibodies are freeze-dried in phosphate buffer with stabilizers and sodium azide, with the exception of horseradish peroxidase conjugates, which do not contain a preservative.

Cross adsorbed secondary antibodies (min X … Sr Prot)

Secondary antibodies against one species are likely to cross-react with other species unless they have been specifically adsorbed against the other species. Antibodies with “(min X … Sr Prot)” in the description have been tested and/or adsorbed against IgG and/or serum proteins of those species indicated in the parentheses. They are recommended when the presence of immunoglobulins from other species may lead to interfering cross-reactivities.

Example AffiniPure™ Anti-Human Secondary Antibodies


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