JIR covid-19

Research Solutions for Coronavirus

Anti-Human Secondary Antibodies

The development of serological tests is critical in the fightback against the novel coronavirus pandemic (covid-19).

 

What is a serological test?

A serological test is used diagnostically to identify antibodies in a sample of blood serum or other bodily fluid. As antibodies are raised as part of the immune response, the presence of an antibody specific for an infectious agent, (e.g. micro-organism or virion) indicates that the patient has (or has had) the infection. Other purposes of serological tests include diagnosis of autoimmune diseases or blood typing.

 

How does a serological test work?

The principle method of a serological test is that the infectious agent is attached to a sample well or other surface, either directly or via a capture antibody. The sample is introduced and if present, antibodies will bind to the infectious agent. Unbound material is washed away, and any bound antibodies are detected with a secondary antibody conjugated to a reporter molecule.

Jackson ImmunoResearch Affinipure Anti-Human secondary antibodies are affinity purified polyclonal antibodies with specificity for human immunoglobulins.

We offer anti-mouse secondary antibodies in a range of host species including Alpaca, Donkey, Goat, Mouse and Rabbit. JIR secondary antibodies are available as whole IgG and F(ab’)2. Fab fragments can be an elegant component of multiple labeling and blocking protocols.

They are available with specificity for whole Ig, Fcy and F(ab’)2 fragment, among other specificities.

Anti-Human Antibodies for Serological Tests

Anti-Human secondary antibodies are an invaluable reagent in the development of high-throughput serological testing kits. JIR Anti-Human antibodies are manufactured to ISO: 2015 certification and are available in standard and bulk quantities for worldwide shipment.

JIR anti-human secondary antibodies are suitable for the detection of human immunoglobulin isotypes by immunoassay including ELISA, ELISPOT, Rapid test, Enzyme immunoassay (EI), Luminscent immunoassay (LIA), Fluorescent immunoassay (FIA) and lateral flow.

 

  • Anti-Human IgG
  • Anti-Human IgM
  • Anti-Human IgA

 

Available with specificities to IgG, IgM or IgA as well as IgG+IgM and IgG+IgM+IgA. Anti-Human products are highly cross-adsorbed.

Host species options include Alpaca, Donkey, Goat, Mouse and Rabbit.

Biotin, HRP and fluorescent dye conjugates available.

Anti-Human Secondary Antibodies are available with the following conjugates:

  • Unconjugated
  • Reporter enzymes (Alkaline Phosphatase (AP) or Horseradish Peroxidase (HRP)
  • Biotinylated (biotin conjugates)
  • Fluorophores (Alexa Fluor®, Brilliant Violet™, Cyanine and protein dye conjugates)
  • Colloidal Gold (Immunogold)

Affinity-purified antibodies are isolated from antisera by immunoaffinity chromatography using target antigens coupled to agarose beads. A proprietary elution process is used to dissociate antibodies from the antigen. Unconjugated affinity-purified antibodies are supplied sterile filtered in phosphate buffer without stabilizers or preservatives. Conjugated affinity-purified antibodies are freeze-dried in phosphate buffer with stabilizers and sodium azide, with the exception of horseradish peroxidase conjugates, which do not contain a preservative.

Cross adsorbed secondary antibodies (min X … Sr Prot)

Secondary antibodies against one species are likely to cross-react with other species unless they have been specifically adsorbed against the other species. Antibodies with “(min X … Sr Prot)” in the description have been tested and/or adsorbed against IgG and/or serum proteins of those species indicated in the parentheses. They are recommended when the presence of immunoglobulins from other species may lead to interfering cross-reactivities.

Popular Affinipure Anti-Human Secondary Antibodies

ProductProduct CodeConjugateCitations*
Goat Anti-Human IgG, Fc gamma Fragment Specific (min X Bov,Hrs,Ms Sr Prot) 109-005-098Unconjugated56
Goat Anti-Human IgG, Fcγ Fragment Specific (min X Bov, Hrs, Ms Sr Prot) 109-035-098HRP46
F(ab’)2 Fragment Goat Anti-Human IgG, Fcγ fragment specific109-116-098R-PE23
Goat Anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr Prot)109-585-088Alexa Fluor® 5943
F(ab’)2 Fragment Donkey Anti-Human IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Ms, Rb, Rat, Shp Sr Prot)709-166-149Cy™37
Donkey Anti-Human IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Ms, Rb, Rat, Shp Sr Prot) 709-605-149Alexa Fluor® 64715

* Number of citations as of 24th March 2020

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