Protein Reagents & Gene Tools

All blocking buffers contain proprietary additives that stabilize the antigenic and functional regions of the coated protein by maintaining the water of hydration during the drying and storage process. These buffers also contain inert additives that block any “sticky” nonspecific binding regions of the adsorbed protein, and any uncoated regions of the polystyrene plate surface. This reduces nonspecific binding of the conjugate and random deposits of sample proteins, thus reducing background noise and increasing the specific signal.

Buffers also include an antimicrobial agent to prevent bacterial growth during the blocking process (which allows blocking to be done at room temperature) and to extend the useful shelf-life of the plates.

Energize your ELISA Assays with our Buffers:

• By saving valuable reagents.
• By promoting a higher specific signal, less protein may be needed to coat the plate and less of the detection molecules may be needed to generate a signal when this buffer is used.
• By increasing the shelf-life of your plates. Depending on the activity of the coated protein, plates may be stored at RT or 2°-8°C for several months, or even years under proper conditions.
• By increasing reproducibility.
• By using consistent reagents from a reliable source, your ELISAs may become more reproducible.
• By increasing the signal of the ELISA. If the antibody or antigen is not sticking to the plate, or unwanted proteins are interfering with binding, the plate will have an overall low specific signal. CB1 may foster adsorption and reduce interference.
• By decreasing background noise. If excess proteins are sticking to the plate, plate will have overall high backgrounds (a high signal overall, but a low specific signal). CB1 may reduce interference.

We offer a group of 6 unique stabilizing diluents designed to prolong the shelf-life of HRP-conjugated proteins and enhance their utility in ELISA applications.

These unique stabilizing buffers designed to prolong the shelf-life of HRP-conjugated proteins and enhance their utility in ELISA applications. These conjugate stabilizer diluents can be used to reconstitute lyophilized conjugates, and to dilute concentrated conjugates into the useful range of the assay.

The proprietary formulations preserve the integrity of the protein conjugate component (for example, the IgG-HRP complex) while also maintaining the enzymatic functionality of the HRP enzyme.

These sample diluents can be used to titrate rabbit, mouse, goat, and chicken serum samples, along with monoclonal cell culture supernatants.  Antigen samples may also be diluted with sample diluents for evaluation in sandwich ELISA formats.

Our customers and collaborators have underscored the importance of providing products and methods that enable improved transfection in-vitro and in-vivo. Our transfection kits are pushing the boundaries.Check out customer publications referencing the delivery of siRNA, shRNA, miRNA, Plasmids and Viruses to cell cultures and the CNS in vivo (via intrathecal, epidural and intraventricular injection).

We will continue to work hard to bring you the transfection reagents needed for your unique applications. For more details, please also visit our Transfection Blog.

These expression vectors offer rapid and highly efficient subcloning of an arbitrary gene – initially cloned into a Donor Vector – to simultaneously fuse it with many different genetic surroundings via transfer into Acceptor Vectors to generate Destination Vectors. The latter enable the efficient expression of your protein with various features (e.g. different tags and different promoters) in different hosts.

Our selection of expression vectors include E. coli expression vectors (pASG-IBA and pASK-IBA vectors), mammalian vectors (pDSG-IBA, pESG-IBA, and pCSG-IBA vectors), yeast expression vectors (pYSG-IBA vectors), Baculo virus vectors (pLSG-IBA vectors), and StarGate entry vectors (pENTRY-IBA51).

We also have a MEXi product portfolio for use in mammalian protein expression, including a HEK293/EBNA-1 cell line and cell culture media.

pASG-IBA vectors are E. coli expression vectors with the following features:

• High-level expression in E. coli, also for toxic proteins
• Tightly regulated expression due to anhydrotetracycline (AHT) inducible tetA promoter/operator
• Option for periplasmic expression due to ompA signal sequence
• No catabolite repression – no influence of medium components
• Not influenced by the genetic background – wide choice of E. coli expression strains
• Inexpensive induction with AHT.

pASK-IBA vectors are expression vectors for recombinant protein expression in E. coli, vectors with the Tet promoter (pASK-IBA), protease cleavage site (Factor Xa) and with chloramphenicol resistance gene are available.

pDSG-IBA vectors are mammalian expression vectors with the following features:

• CMV promoter: high level constitutive expression
• oriP: origin of replication from Epstein-Barr Virus for extrachromosomal replication driven by EBNA-1 (MEXi 293E cells express EBNA-1)
• ColE1 + Amp: for propagation in E. coli
• BM40: secretion signal to transfer the expressed protein into the medium (not on all vectors available)

pESG-IBA vectors are mammalian expression vectors with the following features:

• CMV promoter: high level constitutive expression
• SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
• ColE1 + Amp: for propagation in E. coli
• NeoR: neomycin resistance gene to generate stable cell lines
• BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)

pCSG-IBA vectors are mammalian expression vectors with the following features:

• CMV promoter: high level constitutive expression
• oriP: origin of replication from Epstein Barr Virus for extrachromosomal replication driven by EBNA-1
• EBNA-1 gene: Expression of EBNA-1 for initiation of extrachromosomal replication by oriP
• SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
• NeoR: neomycin resistance gene to generate stable cell lines
• ColE1 + Amp: for propagation in E. coli
• BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)

pYSG-IBA vectors are yeast expression vectors with the following features:

• High-level expression in yeast
• Tightly regulated expression due to Cu++-inducible CUP1 promoter
• Ampicillin resistance gene and markers leu2-d and URA3
• High copy number

pLSG-IBA vectors are baculo virus expression vectors with the following features:

• Cloning and expression of recombinant proteins in insect cells
• Polyhedrin promoter drives high-level expression of the insert
• pUC ori and ampicillin resistance gene support propagation in E. coli

StarGate Entry Vector is required to generate Donor Vectors for subsequent Destination Vector generation. The vector encodes a kanamycin resistance gene.

We also have a MEXi product portfolio for use in mammalian protein expression, including a HEK293/EBNA-1 cell line and cell culture media.

Silica nano-beads has a superparamagnetic Fe3O4 core and hydroxyl outer surface modified with SiO2. It features with -low non-specific adsorption by the optimized size of the microspheres, the thickness of SiO2 layer and -SiOH density. All these features lead to highly pure and effective isolation and purification of nucleic acids including DNA, RNA from E Coli, blood, cells and viruses.

Si-Mag series superparamagnetic silica nano-beads have different sizes range from 80nm to 3 µm to gain diverse binding of small to large DNA molecules. superparamagnetic Fe3O4 as core, low-density silicon modified hydroxy (-SiOH), and a further high specific surface area of the material, making them than conventional magnetic SiO2 microspheres having more excellent nucleic acid binding properties.

The streptavidin:biotin system is widely used for immobilization and detection of biotinylated molecules, like proteins and nucleic acids. Our provides bulk amounts of lyophilized streptavidin with competitive prices in reliable high quality to generate

•    streptavidin coated surfaces (microplates, SPR-chips or beads)

•    detection reagents conjugated with e.g. fluorescent dyes