ELISA Buffers, ELISA Conjugate Diluents, ELISA Assay Diluents, ELISA Sample Diluents, and more
Protein Reagents & Gene Tools
All blocking buffers contain proprietary additives that stabilize the antigenic and functional regions of the coated protein by maintaining the water of hydration during the drying and storage process. These buffers also contain inert additives that block any “sticky” nonspecific binding regions of the adsorbed protein, and any uncoated regions of the polystyrene plate surface. This reduces nonspecific binding of the conjugate and random deposits of sample proteins, thus reducing background noise and increasing the specific signal.
Buffers also include an antimicrobial agent to prevent bacterial growth during the blocking process (which allows blocking to be done at room temperature) and to extend the useful shelf-life of the plates.
Energize your ELISA Assays with our Buffers:
- By saving valuable reagents.
- By promoting a higher specific signal, less protein may be needed to coat the plate and less of the detection molecules may be needed to generate a signal when this buffer is used.
- By increasing the shelf-life of your plates. Depending on the activity of the coated protein, plates may be stored at RT or 2°-8°C for several months, or even years under proper conditions.
- By increasing reproducibility.
- By using consistent reagents from a reliable source, your ELISAs may become more reproducible.
- By increasing the signal of the ELISA. If the antibody or antigen is not sticking to the plate, or unwanted proteins are interfering with binding, the plate will have an overall low specific signal. CB1 may foster adsorption and reduce interference.
- By decreasing background noise. If excess proteins are sticking to the plate, plate will have overall high backgrounds (a high signal overall, but a low specific signal). CB1 may reduce interference.
| Name | Catalog # | Type | Applications | Size |
| 3 Blocking Buffer Sample Pack | KF17340 | Blocking Buffer | E | 3 x 100 ml |
| General Blocking Buffer-BB1 | KF17337 | Blocking Buffer | E | 1 L |
| General Blocking Buffer-BB1 | KF17337 | Blocking Buffer | E | 10 L |
| General Blocking Buffer-BB1 | KF17337 | Blocking Buffer | E | 100 ml |
| General Blocking Buffer-BB1 | KF17337 | Blocking Buffer | E | 500 ml |
| Neptune Blocking Buffer-BB2 | KF17338 | Blocking Buffer | E | 1 L |
| Neptune Blocking Buffer-BB2 | KF17338 | Blocking Buffer | E | 10 L |
| Neptune Blocking Buffer-BB2 | KF17338 | Blocking Buffer | E | 100 ml |
| Neptune Blocking Buffer-BB2 | KF17338 | Blocking Buffer | E | 500 ml |
| Plate Coating Buffer-CB1 | KF17336 | Coating Buffer | E | 1 L |
| Plate Coating Buffer-CB1 | KF17336 | Coating Buffer | E | 10 L |
| Plate Coating Buffer-CB1 | KF17336 | Coating Buffer | E | 100 ml |
| Plate Coating Buffer-CB1 | KF17336 | Coating Buffer | E | 500 ml |
| SynBlock Blocking Buffer-BB3 | KF17339 | Blocking Buffer | E | 1 L |
| SynBlock Blocking Buffer-BB3 | KF17339 | Blocking Buffer | E | 10 L |
| SynBlock Blocking Buffer-BB3 | KF17339 | Blocking Buffer | E | 100 ml |
| SynBlock Blocking Buffer-BB3 | KF17339 | Blocking Buffer | E | 500 ml |
| Wash Buffer | KF17356 | Wash Buffer | E | 1 L |
| Wash Buffer | KF17356 | Wash Buffer | E | 10 L |
| Wash Buffer | KF17356 | Wash Buffer | E | 100 ml |
| Wash Buffer | KF17356 | Wash Buffer | E | 500 ml |
We offer a group of 6 unique stabilizing diluents designed to prolong the shelf-life of HRP-conjugated proteins and enhance their utility in ELISA applications.
These unique stabilizing buffers designed to prolong the shelf-life of HRP-conjugated proteins and enhance their utility in ELISA applications. These conjugate stabilizer diluents can be used to reconstitute lyophilized conjugates, and to dilute concentrated conjugates into the useful range of the assay.
The proprietary formulations preserve the integrity of the protein conjugate component (for example, the IgG-HRP complex) while also maintaining the enzymatic functionality of the HRP enzyme.
| Name | Catalog # | Type | Applications | Size |
| <![CDATA[Monoclonal -Rabbit Polyclonal Conjugate Diluent & Stabilizer-CD5]]> | KF17345 | Conjugate Diluent | E | 1 L |
| <![CDATA[Monoclonal -Rabbit Polyclonal Conjugate Diluent & Stabilizer-CD5]]> | KF17345 | Conjugate Diluent | E | 10 L |
| <![CDATA[Monoclonal -Rabbit Polyclonal Conjugate Diluent & Stabilizer-CD5]]> | KF17345 | Conjugate Diluent | E | 100 ml |
| <![CDATA[Monoclonal -Rabbit Polyclonal Conjugate Diluent & Stabilizer-CD5]]> | KF17345 | Conjugate Diluent | E | 25 ml |
| <![CDATA[Monoclonal -Rabbit Polyclonal Conjugate Diluent & Stabilizer-CD5]]> | KF17345 | Conjugate Diluent | E | 500 ml |
| <![CDATA[Monoclonal-Goat Polyclonal Conjugate Diluent & Stabilizer-CD4]]> | KF17344 | Conjugate Diluent | E | 1 L |
| <![CDATA[Monoclonal-Goat Polyclonal Conjugate Diluent & Stabilizer-CD4]]> | KF17344 | Conjugate Diluent | E | 10 L |
| <![CDATA[Monoclonal-Goat Polyclonal Conjugate Diluent & Stabilizer-CD4]]> | KF17344 | Conjugate Diluent | E | 100 ml |
| <![CDATA[Monoclonal-Goat Polyclonal Conjugate Diluent & Stabilizer-CD4]]> | KF17344 | Conjugate Diluent | E | 25 ml |
| <![CDATA[Monoclonal-Goat Polyclonal Conjugate Diluent & Stabilizer-CD4]]> | KF17344 | Conjugate Diluent | E | 500 ml |
| <![CDATA[Polyclonal-Polyclonal Conjugate Diluent & Stabilizer-CD3]]> | KF17343 | Conjugate Diluent | E | 1 L |
| <![CDATA[Polyclonal-Polyclonal Conjugate Diluent & Stabilizer-CD3]]> | KF17343 | Conjugate Diluent | E | 10 L |
| <![CDATA[Polyclonal-Polyclonal Conjugate Diluent & Stabilizer-CD3]]> | KF17343 | Conjugate Diluent | E | 100 ml |
| <![CDATA[Polyclonal-Polyclonal Conjugate Diluent & Stabilizer-CD3]]> | KF17343 | Conjugate Diluent | E | 25 ml |
| <![CDATA[Polyclonal-Polyclonal Conjugate Diluent & Stabilizer-CD3]]> | KF17343 | Conjugate Diluent | E | 500 ml |
| Conjugate Stock Stabilizing Reagent -CD6 | KF17346 | Conjugate Diluent | E | 1 L |
| Conjugate Stock Stabilizing Reagent -CD6 | KF17346 | Conjugate Diluent | E | 10 L |
| Conjugate Stock Stabilizing Reagent -CD6 | KF17346 | Conjugate Diluent | E | 100 ml |
| Conjugate Stock Stabilizing Reagent -CD6 | KF17346 | Conjugate Diluent | E | 25 ml |
| Conjugate Stock Stabilizing Reagent -CD6 | KF17346 | Conjugate Diluent | E | 500 ml |
These assay diluents are proprietary buffer formulations designed to equalize any differences between the sample matrix (serum, plasma, urine, cell culture fluid) and the diluent used to generate the standard curve. These diluents also reduce nonspecific interactions between the sample matrix proteins and the plate surface which translates into lower background noise.
Assay diluents are pipetted directly onto the plate just prior to adding the samples, and may be used with neat or diluted samples, depending on the characteristics of the sample matrix and the target range of the assay. Since different sample matrices have unique characteristics when run in an ELISA, they must be matched with specific assay diluents. Use of an assay diluent reduces the effects of the sample matrix and variation among samples, without pre-dilution of the samples.
We have 4 proprietary assay diluent formulations for use with sandwich and antigen-down ELISAs:
| Name | Catalog # | Size |
| All Assay Diluents Sample Pack | KF17351 | 4X100 ml |
| Antigen-Down Assay Diluent-AD4 | KF17350 | 1 L |
| Antigen-Down Assay Diluent-AD4 | KF17350 | 10 L |
| Antigen-Down Assay Diluent-AD4 | KF17350 | 100 ml |
| Antigen-Down Assay Diluent-AD4 | KF17350 | 500 ml |
| General Assay Diluent-AD1 | KF17347 | 1 L |
| General Assay Diluent-AD1 | KF17347 | 10 L |
| General Assay Diluent-AD1 | KF17347 | 100 ml |
| General Assay Diluent-AD1 | KF17347 | 500 ml |
| IgM Reducing Assay Diluent-AD2 | KF17348 | 1 L |
| IgM Reducing Assay Diluent-AD2 | KF17348 | 10 L |
| IgM Reducing Assay Diluent-AD2 | KF17348 | 100 ml |
| IgM Reducing Assay Diluent-AD2 | KF17348 | 500 ml |
| Neptune Assay Diluent-AD3 | KF17349 | 1 L |
| Neptune Assay Diluent-AD3 | KF17349 | 10 L |
| Neptune Assay Diluent-AD3 | KF17349 | 100 ml |
| Neptune Assay Diluent-AD3 | KF17349 | 500 ml |
These unique serum sample diluents are specifically formulated for dilution of animal and bird serum samples in antigen-down ELISA formats. Sample diluents are utilized to dilute serum or antigen samples into the functional range of the assay.
Due to the finite binding capacity of the coated ELISA plate surface, certain high samples may overload this capacity, thus requiring dilution prior to testing in the assay. Sample diluents can also reduce background noise associated with nonspecific bridging of signal-generating conjugates to the plate surface.
Hyperimmunized serum samples contain nonspecific IgG antibody that may interfere with the titration signal. Proprietary additives have been incorporated into these diluents that minimize nonspecific binding interactions of the nonspecific IgG in the samples, thus decreasing background noise.
These sample diluents can be used to titrate rabbit, mouse, goat, and chicken serum samples, along with monoclonal cell culture supernatants. Antigen samples may also be diluted with sample diluents for evaluation in sandwich ELISA formats.
| Name | Catalog # | Type | Applications | Size |
| All Sample Diluents Sample Pack | KF17355 | Sample Diluent | E | 3X100 ml |
| General Purpose Sample Diluent-SD1 | KF17352 | Sample Diluent | E | 1 L |
| General Purpose Sample Diluent-SD1 | KF17352 | Sample Diluent | E | 10 L |
| General Purpose Sample Diluent-SD1 | KF17352 | Sample Diluent | E | 100 ml |
| General Purpose Sample Diluent-SD1 | KF17352 | Sample Diluent | E | 500 ml |
| Neptune Sample Diluent-SD3 | KF17354 | Sample Diluent | 1 L | |
| Neptune Sample Diluent-SD3 | KF17354 | Sample Diluent | 10 L | |
| Neptune Sample Diluent-SD3 | KF17354 | Sample Diluent | 100 ml | |
| Neptune Sample Diluent-SD3 | KF17354 | Sample Diluent | 500 ml | |
| Plasma Sample Diluent (Antigen-Down)-SD2 | KF17353 | Sample Diluent | 1 L | |
| Plasma Sample Diluent (Antigen-Down)-SD2 | KF17353 | Sample Diluent | 10 L | |
| Plasma Sample Diluent (Antigen-Down)-SD2 | KF17353 | Sample Diluent | 100 ml | |
| Plasma Sample Diluent (Antigen-Down)-SD2 | KF17353 | Sample Diluent | 500 ml |
Our customers and collaborators have underscored the importance of providing products and methods that enable improved transfection in-vitro and in-vivo. Our transfection kits are pushing the boundaries.Check out customer publications referencing the delivery of siRNA, shRNA, miRNA, Plasmids and Viruses to cell cultures and the CNS in vivo (via intrathecal, epidural and intraventricular injection).
We will continue to work hard to bring you the transfection reagents needed for your unique applications. For more details, please also visit our Transfection Blog.
| Name | Catalog # | Type | Applications | Size |
| DNA-Fect | DF37100-1 | Cationic Ploymer | Transfection Reagent | 1 ml |
| DNA-Fect293 | DF37293-1 | Cationic Ploymer | Transfection Reagent | 1 ml |
| i-Fect | NI35150 | Cationic Lipid | 1 x 0.75 ml | |
| i-Fect | NI35750 | 5 x 0.75 ml | ||
| n-Blast | NB30075 | Cationic Lipid | 0.75 ml | |
| n-Blast | NB30150 | 1.5 ml | ||
| n-Fect | NF30150 | Kit | 1.5 mls | |
| n-Fect | NF30750 | 5 x 1.5 mls | ||
| p-Fect | PF30000 | DNA Diluent | 5×8 mls-DNA Diluent | |
| p-Fect | PF30001 | DNA Diluent | 10×8 mls-DNA Diluent | |
| p-Fect | PF30002 | DNA Diluent | 5×8 mls-DNA Diluent B | |
| p-Fect | PF30003 | DNA Diluent B | 10×8 mls-DNA Diluent B | |
| p-Fect | PF30075 | Cationic Lipid | 75 Reactions | |
| p-Fect | PF30150 | Cationic Lipid | 150 Reactions | |
| p-Fect | PF30750 | Cationic Lipid | 750 Reactions | |
| Penetratin 1 | CP51101 | 500 ugs | ||
| pro-Fect | PT30000 | Cationic Lipid | 24 Reactions | |
| pro-Fect | PT30002 | Cationic Lipid | 96 Reactions | |
| siRNA-intact | SI35100 | RNAse Inhibitor | 100 units in 100 ul |
These expression vectors offer rapid and highly efficient subcloning of an arbitrary gene – initially cloned into a Donor Vector – to simultaneously fuse it with many different genetic surroundings via transfer into Acceptor Vectors to generate Destination Vectors. The latter enable the efficient expression of your protein with various features (e.g. different tags and different promoters) in different hosts.
Our selection of expression vectors include E. coli expression vectors (pASG-IBA and pASK-IBA vectors), mammalian vectors (pDSG-IBA, pESG-IBA, and pCSG-IBA vectors), yeast expression vectors (pYSG-IBA vectors), Baculo virus vectors (pLSG-IBA vectors), and StarGate entry vectors (pENTRY-IBA51).
We also have a MEXi product portfolio for use in mammalian protein expression, including a HEK293/EBNA-1 cell line and cell culture media.
pASG-IBA vectors are E. coli expression vectors with the following features:
- High-level expression in E. coli, also for toxic proteins
- Tightly regulated expression due to anhydrotetracycline (AHT) inducible tetA promoter/operator
- Option for periplasmic expression due to ompA signal sequence
- No catabolite repression – no influence of medium components
- Not influenced by the genetic background – wide choice of E. coli expression strains
- Inexpensive induction with AHT.
| Name | Catalog # | Type | Species | Applications | Size |
| Anhydrotetracycline | 2-0401-001 | Lysis Buffer | Not Applicable | 50 mg | |
| Competent cells E. coli TOP10 | 5-1600-020 | 20 rxns | |||
| pASG-IBA102 | 5-4102-001 | Expression Vector | E. Coli | Gene Transfer | 5 ug |
| pASG-IBA103 | 5-4103-001 | Expression Vector | E. Coli | Gene Transfer | 5 ug |
| pASG-IBA104 | 5-4104-001 | Expression Vector | E. Coli | Gene Transfer | 5 ug |
| pASG-IBA105 | 5-4105-001 | Expression Vector | E. Coli | Gene Transfer | 5 ug |
| pASG-IBA123 | 5-4123-001 | Expression Vector | E. Coli | Gene Transfer | 5 ug |
| pASG-IBA143 | 5-4143-001 | Expression Vector | E. Coli | Gene Transfer | 5 ug |
| pASG-IBA144 | 5-4144-001 | Expression Vector | E. Coli | Gene Transfer | 5 ug |
| pASG-IBA145 | 5-4145-001 | Expression Vector | E. Coli | Gene Transfer | 5 ug |
| pASG-IBA162 | 5-4162-001 | Expression Vector | E. Coli | Gene Transfer | 5 ug |
| pASG-IBA164 | 5-4164-001 | Expression Vector | E. Coli | Gene Transfer | 5 ug |
| pASG-IBA167 vector | 5-4167-001 | Expression Vector | E. Coli | Gene Transfer | 5 µg |
| pASG-IBA168 vector | 5-4168-001 | Expression Vector | E. Coli | Gene Transfer | 5 µg |
| pASG-IBA2 vector | 5-4002-001 | Expression Vector | E. Coli | Gene Transfer | 5 µg |
| pASG-IBA23 vector | 5-4023-001 | Expression Vector | E. Coli | Gene Transfer | 5 µg |
| pASG-IBA3 vector | 5-4003-001 | Expression Vector | E. Coli | Gene Transfer | 5 µg |
| pASG-IBA4 vector | 5-4004-001 | Expression Vector | E. Coli | Gene Transfer | 5 µg |
| pASG-IBA43 vector | 5-4043-001 | Expression Vector | E. Coli | Gene Transfer | 5 µg |
| pASG-IBA44 vector | 5-4044-001 | Expression Vector | E. Coli | Gene Transfer | 5 µg |
| pASG-IBA45 vector | 5-4045-001 | Expression Vector | E. Coli | Gene Transfer | 5 µg |
| pASG-IBA5 vector | 5-4005-001 | Expression Vector | E. Coli | Gene Transfer | 5 µg |
| pASG-IBA62 vector | 5-4062-001 | Expression Vector | E. Coli | Gene Transfer | 5 µg |
| pASG-IBA64 vector | 5-4064-001 | Expression Vector | E. Coli | Gene Transfer | 5 µg |
| pASG-IBAwt1 vector | 5-4000-001 | Expression Vector | E. Coli | Gene Transfer | 5 µg |
| pASG-IBAwt2 vector | 5-4001-001 | Expression Vector | E. Coli | Gene Transfer | 5 µg |
pASK-IBA vectors are expression vectors for recombinant protein expression in E. coli, vectors with the Tet promoter (pASK-IBA), protease cleavage site (Factor Xa) and with chloramphenicol resistance gene are available.
| Name | Catalog # | Type | Species | Applications | Size |
| Anhydrotetracycline | 2-0401-001 | Lysis Buffer | Not Applicable | 50 mg | |
| Competent cells E. coli TOP10 | 5-1600-020 | 20 rxns | |||
| pASK-IBA2C | 2-1321-000 | Expression Vector | E. Coli | Targeted Protein Expression | 5 ug |
| pASK-IBA3C | 2-1322-000 | Expression Vector | E. Coli | Targeted Protein Expression | 5 ug |
| pASK-IBA4C | 2-1323-000 | Expression Vector | E. Coli | Targeted Protein Expression | 5 ug |
| pASK-IBA5C | 2-1324-000 | Expression Vector | E. Coli | Targeted Protein Expression | 5 ug |
| pASK-IBA6C | 2-1325-000 | Expression Vector | E. Coli | Targeted Protein Expression | 5 ug |
| pASK-IBA7C | 2-1326-000 | Expression Vector | E. Coli | Targeted Protein Expression | 5 ug |
pDSG-IBA vectors are mammalian expression vectors with the following features:
- CMV promoter: high level constitutive expression
- oriP: origin of replication from Epstein-Barr Virus for extrachromosomal replication driven by EBNA-1 (MEXi 293E cells express EBNA-1)
- ColE1 + Amp: for propagation in E. coli
- BM40: secretion signal to transfer the expressed protein into the medium (not on all vectors available)
| Name | Catalog # | Type | Species | Applications | Size |
| Competent cells E. coli TOP10 | 5-1600-020 | 20 rxns | |||
| pDSG-IBA102 vector | 5-5219-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pDSG-IBA103 vector | 5-5220-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pDSG-IBA104 vector | 5-5221-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pDSG-IBA105 vector | 5-5222-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pDSG-IBA43 vector | 5-5211-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pDSG-IBA45 vector | 5-5214-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pDSG-IBAwt1 vector | 5-5200-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pDSG-IBAwt2 vector | 5-5201-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
pESG-IBA vectors are mammalian expression vectors with the following features:
- CMV promoter: high level constitutive expression
- SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
- ColE1 + Amp: for propagation in E. coli
- NeoR: neomycin resistance gene to generate stable cell lines
- BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)
| Name | Catalog # | Type | Species | Applications | Size |
| Competent cells E. coli TOP10 | 5-1600-020 | 20 rxns | |||
| pESG-IBA102 vector | 5-4502-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA103 vector | 5-4503-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA104 vector | 5-4504-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA105 vector | 5-4505-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA123 vector | 5-4523-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA142 vector | 5-4542-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA143 vector | 5-4543-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA144 vector | 5-4544-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA145 vector | 5-4545-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA162 vector | 5-4562-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA164 vector | 5-4564-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA167 vector | 5-4567-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA168 vector | 5-4568-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA23 vector | 5-4423-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA3 vector | 5-4403-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA43 vector | 5-4443-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA45 vector | 5-4445-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA5 vector | 5-4405-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA62 vector | 5-4462-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBA64 vector | 5-4464-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBAwt1 vector | 5-4400-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pESG-IBAwt2 vector | 5-4401-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
pCSG-IBA vectors are mammalian expression vectors with the following features:
- CMV promoter: high level constitutive expression
- oriP: origin of replication from Epstein Barr Virus for extrachromosomal replication driven by EBNA-1
- EBNA-1 gene: Expression of EBNA-1 for initiation of extrachromosomal replication by oriP
- SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
- NeoR: neomycin resistance gene to generate stable cell lines
- ColE1 + Amp: for propagation in E. coli
- BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)
| Name | Catalog # | Type | Species | Applications | Size |
| Competent cells E. coli TOP10 | 5-1600-020 | 20 rxns | |||
| pCSG-IBA102 vector | 5-5102-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA103 vector | 5-5103-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA104 vector | 5-5104-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA105 vector | 5-5105-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA123 vector | 5-5123-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA142 vector | 5-5142-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA143 vector | 5-5143-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA144 vector | 5-5144-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA145 vector | 5-5145-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA162 vector | 5-5162-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA164 vector | 5-5164-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA167 vector | 5-5167-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA168 vector | 5-5168-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA23 vector | 5-5023-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA3 vector | 5-5003-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA43 vector | 5-5043-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA45 vector | 5-5045-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA5 vector | 5-5005-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA62 vector | 5-5062-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBA64 vector | 5-5064-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBAwt1 vector | 5-5000-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
| pCSG-IBAwt2 vector | 5-5001-001 | Expression Vector | Mammalian | Gene Transfer | 5 µg |
pYSG-IBA vectors are yeast expression vectors with the following features:
- High-level expression in yeast
- Tightly regulated expression due to Cu++-inducible CUP1 promoter
- Ampicillin resistance gene and markers leu2-d and URA3
- High copy number
| Name | Catalog # | Type | Applications | Size |
| Competent cells E. coli TOP10 | 5-1600-020 | 20 rxns | ||
| pYSG-IBA103 vector | 5-4703-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBA105 vector | 5-4705-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBA123 vector | 5-4723-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBA143 vector | 5-4743-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBA145 vector | 5-4745-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBA162 vector | 5-4762-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBA164 vector | 5-4764-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBA167 vector | 5-4767-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBA168 vector | 5-4768-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBA23 vector | 5-4623-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBA3 vector | 5-4603-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBA43 vector | 5-4643-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBA45 vector | 5-4645-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBA5 vector | 5-4605-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBA62 vector | 5-4662-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBA64 vector | 5-4664-001 | Expression Vector | Gene Transfer | 5 µg |
| pYSG-IBAwt1 vector | 5-4600-001 | Expression Vector | Gene Transfer | 5 µg |
pLSG-IBA vectors are baculo virus expression vectors with the following features:
- Cloning and expression of recombinant proteins in insect cells
- Polyhedrin promoter drives high-level expression of the insert
- pUC ori and ampicillin resistance gene support propagation in E. coli
| Name | Catalog # | Type | Applications | Size |
| Competent cells E. coli TOP10 | 5-1600-020 | 20 rxns | ||
| pLSG-IBA102 vector | 5-4902-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA103 vector | 5-4903-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA104 vector | 5-4904-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA105 vector | 5-4905-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA123 vector | 5-4923-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA142 vector | 5-4942-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA143 vector | 5-4943-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA144 vector | 5-4944-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA145 vector | 5-4945-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA162 vector | 5-4962-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA164 vector | 5-4964-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA167 vector | 5-4967-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA168 vector | 5-4968-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA23 vector | 5-4823-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA3 vector | 5-4803-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA43 vector | 5-4843-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA45 vector | 5-4845-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA5 vector | 5-4805-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA62 vector | 5-4862-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBA64 vector | 5-4864-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBAwt1 vector | 5-4800-001 | Expression Vector | Gene Transfer | 5 µg |
| pLSG-IBAwt2 vector | 5-4801-001 | Expression Vector | Gene Transfer | 5 µg |
StarGate Entry Vector is required to generate Donor Vectors for subsequent Destination Vector generation. The vector encodes a kanamycin resistance gene.
| Name | Catalog # | Type | Applications | Size |
| Competent cells E. coli TOP10 | 5-1600-020 | 20 rxns | ||
| StarGate ENTRY Vector pENTRY-IBA51 | 5-4091-001 | Expression Vector | Gene Transfer | 5 µg |
We also have a MEXi product portfolio for use in mammalian protein expression, including a HEK293/EBNA-1 cell line and cell culture media.
| Name | Catalog # | Type | Species | Applications | Size |
| MEXi-293E cells | 2-6001-001 | Primary Cells | Mammalian | 1.5 ml | |
| MEXi-CM | 2-6010-010 | Cell Growth Media | Cell Assays | 1000 ml | |
| MEXi-TM | 2-6011-010 | Cell Growth Media | Cell Assays | 1000 ml |
The Strep-tag® purification system is based on the highly selective binding of engineered streptavidin, called Strep-Tactin, to Strep-tag II fusion proteins. This technology allows one-step purification of almost any recombinant protein under physiological conditions, thus preserving its bioactivity. The Strep-tag system can be used to purify Strep-tag II proteins from any expression system including baculovirus, mammalian cells, yeast, and bacteria. For vectors for bacterial expression.
Capabilities:
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Simple one-step purification from crude lysate to 99% pure protein under physiological conditions possible.
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High and selective binding affinity and high capacity due to special Strep-Tactin resins
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Resins for gravity flow, low pressure or HPLC applications
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Co-purification of non-covalently bound ligands, thus, protein-protein interaction studies are possible
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Column regeneration and activity status is visualized by color change.
These kit and conjugates are designed for For chemiluminescence detection of Strep-tag proteins on Western blots. They do not require the use of secondary antibodies.
The HRP and AP Detection Kits include all buffers and reagents required for blocking, washing and the chromogenic reaction on a total of 1000 cm2 membrane.
| Name | Catalog # | Type | Species | Applications | Size |
| Strep-Tactin AP Conjugate | 2-1503-001 | Chromogenic Detection | Step-Tactin Proteins | WB | 0.5 ml |
| Strep-Tactin HRP Conjugate | 2-1502-001 | Chromogenic Detection | Step-Tactin Proteins | WB | 0.5 ml |
| Strep-Tactin® coated microplates | 2-1501-001 | Chromogenic Detection | Step-Tactin Proteins | E, Fluoro-Imaging | 10×96 Well Plate |
| Strep-Tactin® coated microplates | 2-1501-001 | Chromogenic Detection | Step-Tactin Proteins | E, Fluoro-Imaging | 1X96 Well Plate |
| Strep-Tactin® coated microplates | 2-1501-001 | Chromogenic Detection | Step-Tactin Proteins | E, Fluoro-Imaging | 5×96 Well Plate |
| Strep-Tactin®XT DY-488 | 2-1562-050 | Protein | 2 x 50 µg | ||
| Strep-Tactin®XT DY-488 | 2-1562-050 | Protein | 5 x 50 µg | ||
| Strep-Tactin®XT DY-488 | 2-1562-050 | Protein | 50 µg | ||
| Strep-Tactin®XT DY-549 | 2-1565-050 | Protein | 2 x 50 µg | ||
| Strep-Tactin®XT DY-549 | 2-1565-050 | Protein | 5 x 50 µg | ||
| Strep-Tactin®XT DY-549 | 2-1565-050 | Protein | 50 µg | ||
| Strep-Tactin®XT DY-649 | 2-1568-050 | Protein | 2 x 50 µg | ||
| Strep-Tactin®XT DY-649 | 2-1568-050 | Protein | 5 x 50 µg | ||
| Strep-Tactin®XT DY-649 | 2-1568-050 | Protein | 50 µg | ||
| StrepMAB-Classic | 2-1507-001 | Mouse IgG | Step-Tactin Proteins | E, IF, FACS | 100 ug |
| StrepMAB-Classic DY-488 | 2-1563-050 | Mouse IgG | 2 x 50 µg | ||
| StrepMAB-Classic DY-488 | 2-1563-050 | Mouse IgG | 5 x 50 µg | ||
| StrepMAB-Classic DY-488 | 2-1563-050 | Mouse IgG | 50 µg | ||
| StrepMAB-Classic DY-549 | 2-1566-050 | Mouse IgG | 2 x 50 µg | ||
| StrepMAB-Classic DY-549 | 2-1566-050 | Mouse IgG | 5 x 50 µg | ||
| StrepMAB-Classic DY-549 | 2-1566-050 | Mouse IgG | 50 µg | ||
| StrepMAB-Classic DY-649 | 2-1569-050 | Mouse IgG | 2 x 50 µg | ||
| StrepMAB-Classic DY-649 | 2-1569-050 | Mouse IgG | 5 x 50 µg | ||
| StrepMAB-Classic DY-649 | 2-1569-050 | Mouse IgG | 50 µg | ||
| StrepMAB-Classic HRP conjugate | 2-1509-001 | Mouse IgG | Step-Tactin Proteins | WB | 75 ul |
| StrepMAB-Immo | 2-1517-001 | Mouse IgG | Step-Tactin Proteins | E, BSC-CM5 | 100 ug |
| StrepMAB-Immo DY-488 | 2-1564-050 | Mouse IgG | 2 x 50 µg | ||
| StrepMAB-Immo DY-488 | 2-1564-050 | Mouse IgG | 5 x 50 µg | ||
| StrepMAB-Immo DY-488 | 2-1564-050 | Mouse IgG | 50 µg | ||
| StrepMAB-Immo DY-549 | 2-1567-050 | Mouse IgG | 2 x 50 µg | ||
| StrepMAB-Immo DY-549 | 2-1567-050 | Mouse IgG | 5 x 50 µg | ||
| StrepMAB-Immo DY-549 | 2-1567-050 | Mouse IgG | 50 µg | ||
| StrepMAB-Immo DY-649 | 2-1570-050 | Mouse IgG | 2 x 50 µg | ||
| StrepMAB-Immo DY-649 | 2-1570-050 | Mouse IgG | 5 x 50 µg | ||
| StrepMAB-Immo DY-649 | 2-1570-050 | Mouse IgG | 50 µg | ||
| Twin-Strep-tag® Capture Kit | 2-4370-000 | 1 kit | |||
| Twin-Strep-tag® Capture Maxi Kit | 2-4370-010 | 1 Kit |
Lysis, Elution, Regeneration, Blocking and Washing Buffers
| Name | Catalog # | Type | Species | Applications | Size |
| Anhydrotetracycline | 2-0401-001 | Lysis Buffer | Not Applicable | 50 mg | |
| Anhydrotetracycline | 2-0401-002 | Cloning | Not Applicable | 25 mg | |
| BioLock Biotin blocking solution | 2-0205-050 | Blocking Buffer | Not Applicable | 50 ml | |
| Biotin Blocking Buffer | 2-0501-002 | Blocking Buffer | Not Applicable | WB | 2 ml |
| Desthiobiotin | 2-1000-002 | 1 g | |||
| Desthiobiotin | 2-1000-005 | Not Applicable | 4 x 5 g | ||
| Desthiobiotin | 2-1000-005 | Not Applicable | 5 g | ||
| Desthiobiotin | 2-1000-001 | Not Applicable | 0.107 g | ||
| GFP-Strep-tag control protein | 2-1006-005 | 500 ug | |||
| GFP-Twin-Strep-tag control protein | 2-1007-005 | Protein | 500 µg | ||
| Strep-tag II Desthiobiotin elution reagent | 2-1000-025 | Elution Buffer | Not Applicable | Protein Purification | 25 ml |
| Strep-tag II Peptide | 2-1018-002 | Peptide | Gravity Flow | 1.8 mg | |
| Strep-tag II Peptide | 2-1018-002 | Peptide | Gravity Flow | 5 x 1.8 mg | |
| Strep-tag protein purification buffer set | 2-1002-001 | Not Applicable | Protein Purification | 1 Buffer Set | |
| Strep-tag regeneration buffer with HABA | 2-1002-100 | Regeneration Buffer | Not Applicable | 100 ml | |
| Strep-tag washing buffer | 2-1003-100 | Wash Buffer | Not Applicable | 100 ml | |
| WET FRED For 1 ml Gravity flow columns | 2-0911-001 | Not Applicable | Cell Culturing System | 1 Set | |
| WET FRED For 5-10 ml Gravity flow columns | 2-0910-001 | Not Applicable | Cell Culturing System | 1 Set |
High affinity meets high capacity!
Key benefits:
- Highest affinity available among affinity purification systems
- Higher protein yields compared to all other Strep-Tactin® resins
- Elution of concentrated protein due to small elution volumes
- Cost efficent: small price for more protein
| Name | Catalog # | Type | Applications | Size |
| 10x Buffer BXT; Strep-Tactin®XT Elution Buffer | 2-1042-025 | Purification | 25 ml | |
| Gravity flow Strep-Tactin®XT Superflow® high capacity column | 2-4032-001 | Column | Purification | 1 ml |
| Gravity flow Strep-Tactin®XT Superflow® high capacity column | 2-4032-001 | Column | Purification | 5 x 1 ml |
| Gravity flow Strep-Tactin®XT Superflow® high capacity column | 2-4033-001 | Column | Protein Purification | 5 ml |
| Gravity flow Strep-Tactin®XT Superflow® high capacity column | 2-4033-001 | Column | Protein Purification | 5 x 5 ml |
| Gravity flow Strep-Tactin®XT Superflow® high capacity mini-column | 2-4031-005 | Column | Protein Purification | 0.2 ml |
| Strep-Tactin® XT Superflow® 50% suspension | 2-4010-002 | Column Suspensions | Protein Purification | 4 ml |
| Strep-Tactin® XT Superflow® 50% suspension | 2-4010-010 | Column Suspensions | Protein Purification, FPLC | 20 ml |
| Strep-Tactin® XT Superflow® 50% suspension | 2-4010-010 | Column Suspensions | Protein Purification, FPLC | 5 x 20 ml |
| Strep-Tactin® XT Superflow® 50% suspension | 2-4010-025 | Column Suspensions | Protein Purification, FPLC | 4 x 50 ml |
| Strep-Tactin® XT Superflow® 50% suspension | 2-4010-025 | Column Suspensions | Protein Purification, FPLC | 50 ml |
| Strep-Tactin®XT 4Flow® cartridge | 2-5023-001 | Protein Purification | 1 ml | |
| Strep-Tactin®XT 4Flow® cartridge | 2-5023-001 | Protein Purification | 10 x 1 ml | |
| Strep-Tactin®XT 4Flow® cartridge | 2-5023-001 | Protein Purification | 5 x 1 ml | |
| Strep-Tactin®XT 4Flow® cartridge | 2-5021-001 | Protein Purification | 1 ml | |
| Strep-Tactin®XT 4Flow® cartridge | 2-5021-001 | Protein Purification | 10 x 1 ml | |
| Strep-Tactin®XT 4Flow® cartridge | 2-5021-001 | Protein Purification | 5 x 1 ml | |
| Strep-Tactin®XT 4Flow® cartridge | 2-5024-001 | Protein Purification | 1 ml | |
| Strep-Tactin®XT 4Flow® cartridge | 2-5024-001 | Protein Purification | 10 x 1 ml | |
| Strep-Tactin®XT 4Flow® cartridge | 2-5024-001 | Protein Purification | 5 x 1 ml | |
| Strep-Tactin®XT 4Flow® high capacity cartridge | 2-5027-001 | Protein Purification | 1 ml | |
| Strep-Tactin®XT 4Flow® high capacity cartridge | 2-5027-001 | Protein Purification | 10 x 1 ml | |
| Strep-Tactin®XT 4Flow® high capacity cartridge | 2-5027-001 | Protein Purification | 5 x 1 ml | |
| Strep-Tactin®XT 4Flow® high capacity cartridge | 2-5028-001 | Protein Purification | 10 x 5 ml | |
| Strep-Tactin®XT 4Flow® high capacity cartridge | 2-5028-001 | Protein Purification | 5 ml | |
| Strep-Tactin®XT 4Flow® high capacity cartridge | 2-5028-001 | Protein Purification | 5 x 5 ml | |
| Strep-Tactin®XT 4Flow® high capacity cartridge | 2-5025-001 | Protein Purification | 1 ml | |
| Strep-Tactin®XT 4Flow® high capacity cartridge | 2-5025-001 | Protein Purification | 10 x 1 ml | |
| Strep-Tactin®XT 4Flow® high capacity cartridge | 2-5025-001 | Protein Purification | 5 x 1 ml | |
| Strep-Tactin®XT 4Flow® high capacity cartridge | 2-5026-001 | Protein Purification | 10 x 5 ml | |
| Strep-Tactin®XT 4Flow® high capacity cartridge | 2-5026-001 | Protein Purification | 5 ml | |
| Strep-Tactin®XT 4Flow® high capacity cartridge | 2-5026-001 | Protein Purification | 5 x 5 ml | |
| Strep-Tactin®XT 4Flow® high capacity column | 2-5031-005 | Protein Purification | 5 x 0.2 ml | |
| Strep-Tactin®XT 4Flow® high capacity column | 2-5032-001 | Protein Purification | 1 ml | |
| Strep-Tactin®XT 4Flow® high capacity column | 2-5032-001 | Protein Purification | 5 x 1ml | |
| Strep-Tactin®XT 4Flow® high capacity column | 2-5033-001 | Protein Purification | 5 ml | |
| Strep-Tactin®XT 4Flow® high capacity column | 2-5033-001 | Protein Purification | 5 x 5 ml | |
| Strep-Tactin®XT 4Flow® high capacity column | 2-5034-001 | Protein Purification | 10 ml | |
| Strep-Tactin®XT 4Flow® high capacity resin | 2-5030-002 | Protein Purification | 4 ml | |
| Strep-Tactin®XT 4Flow® high capacity resin | 2-5030-010 | Protein Purification | 20 ml | |
| Strep-Tactin®XT 4Flow® high capacity resin | 2-5030-010 | Protein Purification | 5 x 20 ml | |
| Strep-Tactin®XT 4Flow® high capacity resin | 2-5030-025 | Protein Purification | 4 x 50 ml | |
| Strep-Tactin®XT 4Flow® high capacity resin | 2-5030-025 | Protein Purification | 50 ml | |
| Strep-Tactin®XT 4Flow® resin | 2-5010-002 | Protein Purification | 4 ml | |
| Strep-Tactin®XT 4Flow® resin | 2-5010-010 | Protein Purification | 20 ml | |
| Strep-Tactin®XT 4Flow® resin | 2-5010-010 | Protein Purification | 5 x 20 ml | |
| Strep-Tactin®XT 4Flow® resin | 2-5010-025 | Protein Purification | 4 x 50 ml | |
| Strep-Tactin®XT 4Flow® resin | 2-5010-025 | Protein Purification | 50 ml | |
| Strep-Tactin®XT Superflow® cartridge | 2-4021-001 | Protein Purification | 1 ml | |
| Strep-Tactin®XT Superflow® cartridge | 2-4021-001 | Protein Purification | 10 x 1 ml | |
| Strep-Tactin®XT Superflow® cartridge | 2-4021-001 | Protein Purification | 5 x 1 ml | |
| Strep-Tactin®XT Superflow® high capacity 50% suspension | 2-4030-002 | Protein Purification | 4 ml | |
| Strep-Tactin®XT Superflow® high capacity 50% suspension | 2-4030-010 | Protein Purification | 20 ml | |
| Strep-Tactin®XT Superflow® high capacity 50% suspension | 2-4030-010 | Protein Purification | 5 x 20 ml | |
| Strep-Tactin®XT Superflow® high capacity cartridge | 2-4026-001 | Protein Purification | 10 x 5 ml | |
| Strep-Tactin®XT Superflow® high capacity cartridge | 2-4026-001 | Protein Purification | 5 ml | |
| Strep-Tactin®XT Superflow® high capacity cartridge | 2-4026-001 | Protein Purification | 5 x 5 ml | |
| Strep-Tactin®XT Superflow® high capacity cartridge | 2-4025-001 | Purification | 1 x 1 ml | |
| Strep-Tactin®XT Superflow® high capacity cartridge | 2-4025-001 | Purification | 10 x 1 ml | |
| Strep-Tactin®XT Superflow® high capacity cartridge | 2-4025-001 | Purification | 5 x 1 ml | |
| Strep-Tactin®XT Superflow® high capacity column | 2-4034-001 | Protein Purification | 10 ml | |
| Strep-Tactin®XT Superflow® high capacity resin | 2-4030-025 | Protein Purification | 4 x 50 ml | |
| Strep-Tactin®XT Superflow® high capacity resin | 2-4030-025 | Protein Purification | 50 ml |
The newly engineered Strep-Tactin®XT has an improved binding affinity to Twin-Strep-tag® and Strep-tag®II and is more stable compared to Strep-Tactin®. Strep-Tactin®XT now enables new applications in the field of high throughput screening, batch purification, purification using denaturing conditions and protein interaction studies. It also provides higher protein yields compared to Strep-Tactin®. Simultaneously, Strep-Tactin®XT preserves all benefits of Strep-Tactin® for protein purification, e.g. high purity (> 95 %), physiological buffer conditions, mild elution, regeneration of the resin for re-use.
Strep-Tactin XT:Twin-Srep-tag is the most versatile affinity purification system and can be used for all kind of applications.
| Name | Catalog # | Type | Applications | Size |
| Gravity flow Strep-Tactin® XT Superflow® column | 2-4012-001 | Column | Protein Purification | 1 x 1 ml |
| Gravity flow Strep-Tactin® XT Superflow® column | 2-4012-001 | Column | Protein Purification | 5 x 1 ml |
| Gravity flow Strep-Tactin® XT Superflow® column | 2-4013-001 | Column | Protein Purification | 1 x 5 ml |
| Gravity flow Strep-Tactin® XT Superflow® column | 2-4013-001 | Column | Protein Purification | 5 x 5 ml |
| Gravity flow Strep-Tactin® XT Superflow® column | 2-4014-001 | Column | Protein Purification | 1 x 10 ml |
| Magnetic Separator | 2-1602-000 | 1 Piece | ||
| MagStrep “type3” XT beads 5% suspension | 2-4090-002 | 2 ml | ||
| MagStrep “type3” XT beads 5% suspension | 2-4090-002 | 3 x 2 ml | ||
| MagStrep “type3” XT beads 5% suspension | 2-4090-002 | 5 x 2 ml | ||
| Strep-Tactin®XT 4Flow® column | 2-5012-001 | Protein Purification | 1 ml | |
| Strep-Tactin®XT 4Flow® column | 2-5012-001 | Protein Purification | 5 x 1 ml | |
| Strep-Tactin®XT 4Flow® column | 2-5013-001 | Purification | 5 ml | |
| Strep-Tactin®XT 4Flow® column | 2-5013-001 | Purification | 5 x 5 ml | |
| Strep-Tactin®XT 4Flow® column | 2-5014-001 | Protein Purification | 10 ml | |
| Strep-Tactin®XT 4Flow® column | 2-5011-005 | Protein Purification | 0.2 ml | |
| Strep-Tactin®XT 4Flow® Starter Kit | 2-5998-000 | Protein Purification | 1 kit | |
| Strep-Tactin®XT Superflow® column | 2-4011-005 | Protein Purification | 0.2 ml |
Five Strep-Tactin resin versions are available which differ in their properties and applications. While Strep-Tactin Sepharose® is preferentially used for gravity flow chromatography, Strep-Tactin® Superflow®, Strep-Tactin® Superflow® high capacity and Strep-Tactin MacroPrep® can also be used for low pressure or FPLC applications, and Strep-Tactin POROS® (20 and 50) for FPLC and HPLC chromatography. Each matrix exhibits different non-specific binding properties.
In cases where unsatisfactory results are obtained with one matrix due to unspecific background, it is advisable to switch to one of the other matrices. In addition, Superflow is especially suited for increased flow rates and is also useful for the purification of large protein complexes. Please note, that the Strep-Tactin resins are optimized for column affinity chromatography as opposed to batch purification
| Name | Catalog # | Type | Applications | Size |
| Strep-Tactin Sepharose 50% suspension | 2-1201-002 | Column Suspensions | Protein Purification | 4 ml |
| Strep-Tactin Sepharose 50% suspension | 2-1201-010 | Column Suspensions | Protein Purification | 20 ml |
| Strep-Tactin Sepharose 50% suspension | 2-1201-010 | Column Suspensions | Protein Purification | 5 x 20 ml |
| Strep-Tactin Sepharose 50% suspension | 2-1201-025 | Column Suspensions | Protein Purification | 4 x 50 ml |
| Strep-Tactin Sepharose 50% suspension | 2-1201-025 | Column Suspensions | Protein Purification | 50 ml |
| Strep-Tactin Superflow 50% suspension | 2-1206-025 | Column Suspensions | Protein Purification | 4 x 50 ml |
| Strep-Tactin Superflow 50% suspension | 2-1206-025 | Column Suspensions | Protein Purification | 50 ml |
| Strep-Tactin® Sepharose Gravityflow-1 ml | 2-1202-001 | Column | Protein Purification | 1 ml |
| Strep-Tactin® Sepharose Gravityflow-1 ml | 2-1202-001 | Column | Protein Purification | 5 x 1 ml |
| Strep-Tactin® Sepharose Gravityflow-10 ml | 2-1202-101 | Column | Protein Purification | 10 ml |
| Strep-Tactin® Sepharose Gravityflow-5 ml | 2-1202-051 | Column | Protein Purification | 5 ml |
| Strep-Tactin® Sepharose Gravityflow-5 ml | 2-1202-051 | Column | Protein Purification | 5 x 5 ml |
| Strep-Tactin® Superflow® high capacity 50% suspension | 2-1208-002 | Column Suspensions | Protein Purification | 4 ml |
| Strep-Tactin® Superflow® high capacity 50% suspension | 2-1208-010 | 20 ml | ||
| Strep-Tactin® Superflow® high capacity 50% suspension | 2-1208-010 | 5 x 20 ml | ||
| Strep-Tactin® Superflow® high capacity 50% suspension | 2-1208-025 | 4 x 50 ml | ||
| Strep-Tactin® Superflow® high capacity 50% suspension | 2-1208-025 | 50 ml | ||
| Strep-Tactin® Superflow® high capacity cartridge | 2-1237-001 | Protein Purification | 1 ml | |
| Strep-Tactin® Superflow® high capacity cartridge | 2-1237-001 | Protein Purification | 10 x 1 ml | |
| Strep-Tactin® Superflow® high capacity cartridge | 2-1237-001 | Protein Purification | 5 x 1 ml | |
| Strep-Tactin® Superflow® high capacity cartridge | 2-1238-001 | Protein Purification | 10 x 5 ml | |
| Strep-Tactin® Superflow® high capacity cartridge | 2-1238-001 | Protein Purification | 5 ml | |
| Strep-Tactin® Superflow® high capacity cartridge | 2-1238-001 | Protein Purification | 5 x 5 ml | |
| Strep-Tactin® Superflow® high capacity cartridge | 2-1239-001 | Protein Purification | 1 ml | |
| Strep-Tactin® Superflow® high capacity cartridge | 2-1239-001 | Protein Purification | 10 x 1 ml | |
| Strep-Tactin® Superflow® high capacity cartridge | 2-1239-001 | Protein Purification | 5 x 1 ml | |
| Strep-Tactin® Superflow® high capacity cartridge | 2-1240-001 | Protein Purification | 10 x 5 ml | |
| Strep-Tactin® Superflow® high capacity cartridge | 2-1240-001 | Protein Purification | 5 ml | |
| Strep-Tactin® Superflow® high capacity cartridge | 2-1240-001 | Protein Purification | 5 x 5 ml | |
| Strep-Tactin® Superflow-10 ml | 2-1209-101 | Column | Protein Purification, FPLC | 10 ml |
| Strep-Tactin® Superflow-1X1 ml | 2-1209-001 | Column | Protein Purification, FPLC | 1 ml |
| Strep-Tactin® Superflow-1X1 ml | 2-1209-001 | Column | Protein Purification, FPLC | 10 x 1 ml |
| Strep-Tactin® Superflow-1X1 ml | 2-1209-001 | Column | Protein Purification, FPLC | 5 x 1 ml |
| Strep-Tactin® Superflow-5 ml | 2-1209-051 | Column | Protein Purification, FPLC | 5 ml |
| Strep-Tactin® Superflow-5 ml | 2-1209-051 | Column | Protein Purification, FPLC | 5 x 5 ml |
| Strep-Tactin®XT Spin Column Kit | 2-4151-000 | Protein Purification | 1 kit | |
| Strep-Tactin®XT Spin Columns | 2-4150-025 | Protein Purification | 25 Columns |
| Name | Catalog # | Type | Applications | Size |
| Avidin | 2-0204-015 | Protein | 15 mg | |
| Avidin | 2-0204-050 | Protein | 50 mg | |
| BioLock Biotin blocking solution | 2-0205-250 | Blocking Buffer | 250 ml | |
| Buffer XT-R | 2-1045-250 | Regeneration Buffer | 250ml | |
| MagStrep “type3” XT beads 5% suspension | 2-4090-010 | 10 ml | ||
| MagStrep “type3” XT beads 5% suspension | 2-4090-010 | 10 x 10 ml | ||
| MagStrep “type3” XT beads 5% suspension | 2-4090-010 | 5 x 10 ml | ||
| Strep-Tactin® | 2-1204-001 | 1 mg | ||
| Strep-Tactin® | 2-1204-005 | 5 mg | ||
| Strep-Tactin® MacroPrep® resin | 2-1505-010 | Protein Purification | 20 ml | |
| Strep-Tactin® MacroPrep® resin | 2-1505-010 | Protein Purification | 5 x 20 ml | |
| Strep-Tactin® XT coated microplate | 2-4101-001 | 1 plate | ||
| Strep-Tactin® XT coated microplate | 2-4101-001 | 100 x 1 plate | ||
| Strep-Tactin® XT coated microplate | 2-4101-001 | 5 x 1 plate | ||
| Strep-Tactin® XT coated microplate | 2-4101-001 | 50 x 1 plate | ||
| Strep-Tactin®XT Buffer Set | 2-1043-000 | 1 Set | ||
| WET FRED For 1 ml Gravity flow columns | 2-0911-001 | Cell Culturing System | 1 Set | |
| WET FRED For 5-10 ml Gravity flow columns | 2-0910-001 | Cell Culturing System | 1 Set |
Silica nano-beads has a superparamagnetic Fe3O4 core and hydroxyl outer surface modified with SiO2. It features with -low non-specific adsorption by the optimized size of the microspheres, the thickness of SiO2 layer and -SiOH density. All these features lead to highly pure and effective isolation and purification of nucleic acids including DNA, RNA from E Coli, blood, cells and viruses.
Si-Mag series superparamagnetic silica nano-beads have different sizes range from 80nm to 3 µm to gain diverse binding of small to large DNA molecules. superparamagnetic Fe3O4 as core, low-density silicon modified hydroxy (-SiOH), and a further high specific surface area of the material, making them than conventional magnetic SiO2 microspheres having more excellent nucleic acid binding properties.
| Name | Catalog # | Type | Applications | Size |
| Agarose Gel DNA Extraction and Purification | EP10010 | Kit | DNA Extraction/Purification | 100 Tests |
| Genomic DNA Extraction and Purification | EP10012 | Kit | DNA Extraction/Purification | 100 Tests |
| PCR DNA Extraction and Purification | EP10013 | Kit | DNA Extraction/Purification | 100 Tests |
| Plasmid DNA Extraction and Purification | EP10014 | Kit | DNA Extraction/Purification | 100 Tests |
| Si-Mag Magnet | EP10016 | DNA Extraction/Purification | 1 Magnet Rack | |
| Tissue and Cell Extraction and Purification | EP10011 | Kit | DNA Extraction/Purification | 100 Tests |
| Virus DNA extraction and purification | EP10015 | Kit | DNA Extraction/Purification | 100 Tests |
The streptavidin:biotin system is widely used for immobilization and detection of biotinylated molecules, like proteins and nucleic acids. Our provides bulk amounts of lyophilized streptavidin with competitive prices in reliable high quality to generate
• streptavidin coated surfaces (microplates, SPR-chips or beads)
• detection reagents conjugated with e.g. fluorescent dyes
| Name | Catalog # | Type | Size |
| Streptavidin | 2-0203-001 | Protein | 1 mg |
| Streptavidin | 2-0203-010 | Protein | 10 mg |
| Streptavidin | 2-0203-100 | Protein | 10 x 100 mg |
| Streptavidin | 2-0203-100 | Protein | 100 mg |
| Streptavidin | 2-0203-100 | Protein | 100 x 100 mg |
| Streptavidin | 2-0203-100 | Protein | 5 x 100 mg |
| Streptavidin | 2-0203-100 | Protein | 50 x 100 mg |
| Streptavidin | 2-0203-101 | Protein | 1 g |
| Streptavidin | 2-0203-101 | Protein | 10 x 1 g |
| Streptavidin | 2-0203-101 | Protein | 5 x 1 g |
| Streptavidin | 2-0203-105 | Protein | 2 x 5 g |
| Streptavidin | 2-0203-105 | Protein | 5 g |
| Streptavidin | 2-0203-105 | Protein | 2 x 5 g |
| Streptavidin | 2-0203-105 | Protein | 5 g |
Selector resins are based on high-affinity single-domain antibodies (sdAb) which are covalently immobilized on 4% cross-linked agarose beads. The oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no “leakage” of light and heavy chains from IgGs is observed during elution with SDS sample buffer.
Key Benefits of Selector Resins
- High capacity
- Minimal unspecific binding
- No co-elution of heavy and light chain
- Broad buffer compatibility for high flexibility
| Name | Catalog # | Species | Size |
| GFP Selector | 2-9131-020 | 2 ml | |
| GST Selector | 2-9121-020 | 2 ml | |
| MBP Selector | 2-9111-020 | 2 ml | |
| Mini Spin Columns | 2-9102-010 | Not Applicable | 10 columns |
| Mini Spin Columns | 2-9102-050 | Not Applicable | 50 columns |
| RFP Selector | 2-9141-020 | 2 ml | |
| Selector Control | 2-9101-004 | 400 µl | |
| TagFP Selector | 2-9151-020 | 2 ml |
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