Sino Biological COVID-19

Research Solutions for Coronavirus

SARS-CoV-2 (2019-nCoV) Detection Kits, Antibodies and Proteins

The newly identified coronavirus, SARS-CoV-2 (2019-nCoV), has led to pneumonia (COVID-19) that sickened over 240,000 people worldwide. SARS-CoV-2 belongs to the Betacoronavirus Genus, which also includes SARS CoV (2003) and MERS CoV (2012). Same as all other coronaviruses, the genome of SARS-CoV-2 (2019-nCoV) encodes the spike protein, the envelope protein, the membrane protein, and the nucleocapsid protein.
The spike protein (S-protein) mediates receptor binding and membrane fusion. Spike protein contains two subunits, S1 and S2. S1 contains a receptor binding domain (RBD), which is responsible for recognizing and binding with the cell surface receptor. S2 subunit is the “stem” of the structure, which contains other basic elements needed for the membrane fusion. The spike protein is the common target for neutralizing antibodies and vaccines. It’s been reported that SARS-CoV-2 (2019-nCoV) (ref.) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. Indeed, the recombinant Spike protein can bind with recombinant ACE2 protein.
The Nucleocapsid Protein (N-protein) is the most abundant protein in coronavirus. The N-protein is a highly immunogenic phosphoprotein, and it is normally very conserved. The N protein of coronavirus is often used as a marker in diagnostic assays.
To aid the efforts of developing vaccines and neutralizing antibodies against this virus, Sino Biological Inc. has developed a panel of research reagents for SARS-CoV-2, including recombinant antigens (the N protein, S protein, the S1 and S2 subunits of the S protein, and the RBD domain of the S proteins), antibodies, antigen detection kits and genes.

SARS-CoV-2 Antigens

N SARS-CoV-2 (2019-nCoV) Nucleocapsid-His Protein 40588-V08B Expresed in Insect cell
S1 SARS-CoV-2 (2019-nCoV) Spike S1-His Protein 40591-V08B Expresed in HEK293 cell, bind with ACE2
S1 SARS-CoV-2 (2019-nCoV) Spike S1-His Protein 40591-V08B1 Expresed in Insect cell, bind with ACE2
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein 40592-V05H Expresed in HEK293 cell, bind with ACE2
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-Fc Protein 40592-V02H Expresed in HEK293 cell, bind with ACE2
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-rFc Protein 40592-V31H Expresed in HEK293 cell, bind with ACE2
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein, Biotinylated 40592-V08B-B Expresed in Insect cell
S2 SARS-CoV-2 (2019-nCoV) Spike S2-mFc Protein 40592-V05H Expresed in HEK293 cell
S1+S2 SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Protein 40589-V08B1 Expresed in Insect cell, bind with ACE2
S1 SARS-CoV-2 (2019-nCoV) Spike S1-mFc Protein 40591-V05H1 Expresed in HEK293 cell, bind with ACE2
S1 SARS-CoV-2 (2019-nCoV) Spike S1-Fc Protein 40591-V02H Expresed in HEK293 cell, bind with ACE2
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein 40592-V08B Expresed in Insect cell, bind with ACE2
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein 40592-V08H Expresed in HEK293 cell, bind with ACE2
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein,Biotinylated 40592-V08H-B Expresed in HEK293 cell
S2 SARS-CoV-2 (2019-nCoV) Spike S2 ECD-His Protein 40590-V08B Expresed in Insect cell
Plpro SARS-CoV-2 (2019-nCoV) Plpro-His protein 40593-V07E Expresed in E.coli cell

SARS-CoV-2 Antibodies

Research Solutions for Coronavirus
Research Solutions for Coronavirus
Cat# Antigen Description Type
40588-T62 Nucleocapsid SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody Rabbit PAb
40143-R001 Nucleocapsid SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody Rabbit MAb
40143-MM05 Nucleocapsid SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody Mouse MAb
40143-R019 Nucleocapsid SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody Rabbit MAb
40143-MM08 Nucleocapsid SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody Mouse MAb
40143-R040 Nucleocapsid SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody Rabbit MAb
40143-R004 Nucleocapsid SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody Rabbit MAb
40150-R007 Spike SARS-CoV-2 (2019-nCoV) Spike S1 Antibody Rabbit MAb
40150-D006 Spike SARS-CoV/SARS-CoV-2 Spike Antibody Chimeric MAb
40150-D004 Spike SARS-CoV/SARS-CoV-2 Spike antibody Chimeric MAb
40150-D003 Spike SARS-CoV/SARS-CoV-2 Spike antibody Chimeric MAb
40150-D005 Spike SARS-CoV/SARS-CoV-2 Spike antibody Chimeric MAb
40150-D002 Spike SARS-CoV/SARS-CoV-2 Spike antibody Chimeric MAb
40150-D001 Spike SARS-CoV/SARS-CoV-2 Spike antibody Chimeric MAb
40592-T62 Spike SARS-CoV-2 (2019-nCoV) Spike RBD Antibody Rabbit PAb
40590-T62 Spike SARS-CoV-2 (2019-nCoV) Spike S2 Antibody Rabbit PAb

Validated Applications

IF (40150-R007) – Pseudovirus Validated

Research Solutions for Coronavirus

SARS-CoV-2 Spike in ACE2-overexpressed 293T cells, infected (left) or noninfected (right) by 2019-nCOV pseudovirus

WB (40143-MM05)

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1-2: SARS-CoV N Protein
3-4: 2019-nCoV N Protein

IHC (40143-R019)

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Renal tissue from COVID-19 Patient. N antigens in kidney tubules is validated by IHC

The S and N detection kits employ a standard sandwich ELISA, allowing rapid quantification of the N and S antigens.

  • All antibodies used in the kits are in-house developed monoclonal antibodies, ensuring batch-to-batch consistency.
  • These assays may be used to quantify antigens on the surface of SARS-CoV-2 and/or pseudovirus, as well as antigens expressed recombinantly.

2019-nCoV N Detection Kit
(KIT40588)

Research Solutions for Coronavirus

Catalogue

Species

Antigen

Type

Description

40592-T62

SARS-CoV-2

S RBD

Rabbit PAb

WB, ELISA, IHC-PF CM, ICC/IF, IP

40588-T62

SARS-CoV-2

Nucleocapsid

Rabbit PAb

WB, ELISA, IHC-PF CM, ICC/IF, IP

40590-T62

SARS-CoV-2

S2 subunit

Rabbit PAb

WB, ELISA, IHC-PF CM, ICC/IF, IP

40143-T62

SARS-CoV

Nucleocapsid

Rabbit PAb

Has cross-reactivity in ELISA with SARS-CoV-2 N Protein (Cat# 40588-V08B).

40150-T62-COV2

SARS-CoV

Spike

Rabbit PAb

Has cross-reactivity in ELISA and WB with SARS-CoV-2 S1 (Cat# 40591-V08H) and S-RBD (Cat# 40592-V05H)

Sino Biological and Nanommune (Irvine, California) jointly developed the coronavirus antigen array. These arrays are constructed by printing recombinant antigens on a nitrocellulose-coated membrane. It can analyze serum antibodies against 65 antigens from 23 types of viruses known to cause respiratory tract infections, including SARS-COV-2 and the other six coronaviruses. The array is specifically designed for high throughput serological surveillance studies.

Catalogue

Description

Species

Tag

Vector

VG40588-UT

CoV Nucleocapsid
2019-nCoV

2019-nCoV

No Tag

Expression

VG40589-UT

CoV spike
2019-nCoV

2019-nCoV

No Tag

Expression

VG40592-UT

CoV spike
2019-nCoV

2019-nCoV

No Tag

Expression

VG40590-UT

CoV spike
2019-nCoV

2019-nCoV

No Tag

Expression

VG40591-UT

CoV spike
2019-nCoV

2019-nCoV

No Tag

Expression

VG40596-UT

CoV Hemagglutinin esterase

2019-nCoV

No Tag

Expression

VG40598-UT

CoV Membrane

2019-nCoV

No Tag

Expression

VG40599-UT

CoV NSP10

2019-nCoV

No Tag

Expression

VG40594-UT

CoV 3CLpro / 3C-like protease

2019-nCoV

No Tag

Expression

VG40597-UT

CoV Envelope

2019-nCoV

No Tag

Expression

VG40593-UT

CoV NSP3

2019-nCoV

No Tag

Expression

Research Solutions for Coronavirus

SUPPORT

outstanding technical support

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PRODUCT

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Research Solutions for Coronavirus

DELIVERY

we offer free delivery to UK universities and non profit organisations