Technical Tips

Technical Tips for Secondary Antibodies

Simultaneous detection of more than one antigen depends on: Secondary antibodies that do not; recognise one another recognise other primary Read More…

Affinity-Purified Secondary Antibodies

Affinity purified antibodies are isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. A proprietary elution process Read More…

Cy2, Cy3 and Cy5 Conjugates for Permanent Mounting

Among currently available fluorescent dyes, the cyanine dyes are better able to withstand the harsh dehydration and embedding conditions required Read More…

Weak Signals from Labelled Secondary Antibodies

  What are the possible causes of weak signals from labelled secondary antibodies assuming that antigens are present and primary Read More…

Probes Conjugated to Affinity-Purified Antibodies and to Other Proteins

Fluorescent Dyes Selection of fluorophores depends on: Instrument set‑up. Examples include availability of light sources, filter sets and detection systems. Read More…

Alexa Fluor® 680 & Alexa Fluor® 790 for Far-red and Infrared Detection on Western Blots

Alexa Fluor® 680 is a far-red-emitting dye with peak excitation at 684 nm and peak emission at 702 nm.  Alexa Read More…

Normal Serums & Gamma Globulins from Jackson ImmunoResearch

Jackson ImmunoResearch provides a range of normal serums and gamma globulins for research use. Normal serums are lipid extracted to Read More…