EpiCypher recognizes the demand for reliable profiling from low cell numbers. In fact, CUT&Tag was specifically developed by the Henikoff lab to enable high-quality, cost-effective chromatin mapping from ultra-low cell inputs1. The use of tagmentation and the Direct-to-PCR protocol eliminates multiple assay steps, reducing the risk of sample loss and making CUTANA CUT&Tag well suited for low sample inputs.
EpiCypher has validated our CUT&Tag Kit for reliable profiling using 100,000 to 10,000 nuclei and has the data to back it up. As shown in Figure 3, EpiCypher’s CUT&Tag kit generates reliable data for histone PTM targets of high (H3K27me3) and low (H3K4me1) abundance. Our kit manual provides detailed guidance and considerations for reducing cell inputs.
*What about inputs below 10,000 nuclei/cells? Although CUT&Tag has been used for single cell or low-input profiling1,3,5-9, these data were generated using microfluidics platforms and/or combinatorial barcoding. With the CUTANA CUT&Tag Kit, most users require at least 10,000 nuclei for reproducible CUT&Tag profiles. Note that success at low inputs is highly dependent on antibody specificity and efficiency, target abundance in your cell type, and the quality of sample preparation.
Figure 3: The CUTANA CUT&Tag Kit yields robust, reliable profiles down to 10,000 nuclei. Representative genome browser tracks are shown for H3K4me1 (low abundance target; EpiCypher Cat No. 13-0057) and H3K27me3 (high abundance target; EpiCypher Cat No. 13-0055). CUT&Tag data were generated by EpiCypher using decreasing amounts of K562 nuclei and 5-8 million sequencing reads per reaction.