EpiCypher offers cutting edge tools and service for epigenetics and chromatin biology research. We pioneered the manufacture of designer recombinant nucleosomes that contain defined post-translational modifications. Our latest product, SNAP-ChIP, is the gold standard for antibody validation for chromatin IP, and also serves as the most reproducible spike-in for normalizing ChIP and ChIP-seq assays. EpiCypher also develops assays for chromatin-modifying enzymes and PTM-binding proteins using a variety of readouts, such as Alpha, FRET and radioactivity, employing nucleosome or peptide-based substrates.


Product Categories

Featured Technologies

EpiCypher is dedicated to creating novel platforms that support innovative chromatin research. We are constantly expanding our product lines to provide the latest in quantitative chromatin technology.

New CUTANA CUNT&RUN
Compatible Antibodies

Extensively validated. Meticulously optimized.

NEW! CUTANA™
ChIC / CUT&RUN Kit

Extensively validated. Meticulously optimized.

CUTANA™ CUT&Tag Assays

Ultra-sensitive genomic profiling assays with lower input and sequencing read depth requirements than ChIP

SNAP-ChIP™ spike-ins

Accurate ChIP-seq antibody validation and data normalization using DNA-barcoded nucleosome spike-ins

CUTANA™ ChIC / CUT&RUN Assays

Robust, high-resolution chromatin profiling, at a fraction of the time and cost of ChIP-seq

Nucleosomes

rNucs Human Recombinant Nucleosomes, No PTMs

NameCat. No.
Mononucleosomes, biotinylated 16-0006
Mononucleosomes, non-biotinylated 16-0009
Mononucleosomes, desthiobiotinylated 16-0024
Tailless Nucleosomes, biotinylated 16-0027
Mononucleosomes, H3.1 ΔN2, biotinylated 16-0023
Mononucleosomes, H3.1 ΔN32, biotinylated16-0016
Mononucleosomes, H3.1 ΔN32, non-biotinylated 16-1016
Mononucleosomes, H3.3 ΔN32, biotinylated 16-0017
Mononucleosomes, H3.3 ΔN32, non-biotinylated16-0017
Mononucleosomes, H4 ΔN15, biotinylated 16-0018

dNucs Designer Recombinant Nucleosomes with PTMs

Lysine Methylation

NameCat#NameCat#
H3K4me1, biotinylated16-0321H3K36me1, biotinylated16-0322
H3K4me2, biotinylated16-0334H3K36me2, biotinylated16-0319
H3K4me2, non-biotinylated16-1334H3K36me2, non-biotinylated16-1319
H3K4me3, biotinylated16-0316H3K36me3, biotinylated16-0320
H3K4me3, non-biotinylated16-1316H3K36me3, non-biotinylated16-1320
H3K4me3,K9,14,18ac16-0335H3.3K36me3, biotinylated16-0390
H3K9me1, biotinylated16-0325H3K79me1, biotinylated 16-0367
H3K9me2, biotinylated16-0324H3K79me2, biotinylated 16-0368
H3K9me3, biotinylated16-0315H3K79me3, biotinylated 16-0369
H3K27me1, biotinylated16-0338H4K12me1, biotinylated 16-0393
H3K27me2, biotinylated16-0339H4K20me1, biotinylated16-0331
H3K27me3, biotinylated16-0317H4K20me2, biotinylated16-0332
H3K27me3, non-biotinylated16-1317H4K20me3, biotinylated16-0333
H4K20me3, non-biotinylated16-1333

Lysine Acylation

NameCat#NameCat#
H2AK5,9,13,15ac, biotinylated16-0376H3K27bu, biotinylated16-0384
H2BK12ac, biotinylated16-0386H3K27cr, biotinylated 16-0383
H2BK20ac, biotinylated16-0387H3K27ac, S28phos, biotinylated16-0385
H3K4ac, biotinylated16-0342H3K36ac, biotinylated 16-0378
H3K9ac, biotinylated16-0314H4K5ac, biotinylated 16-0352
H3K9ac, non-biotinylated16-1314H4K8ac, biotinylated 16-0353
H3K9bu, biotinylated 16-0371H4K12ac, biotinylated16-0312
H3K9cr, biotinylated16-0351H4K16ac, biotinylated16-0354
H3K14ac, biotinylated16-0343H4K20ac, biotinylated 16-0377
H3K18ac, biotinylated 16-0372H2AK5,9,13,15ac, biotinylated16-0376
H3K18bu, biotinylated 16-0373H3K4,9,14,18ac, biotinylated 16-0336
H3K18cr, biotinylated16-0337H3K4me3, K9,14,18ac, biotinylated 16-0335
H3K23ac, biotinylated 16-0364H4K5,8,12,16ac, biotinylated16-0313
H3K27ac, biotinylated16-0365H4K5,8,12,16ac, non-biotinylated16-1313
H3K27ac, non-biotinylated16-1365H3K4,9,14,18ac / H4K5,8,12,16ac, biotinylated16-0374
H3K27acS28phos, biotinylated16-0385

Arginine Methylation

NameCat#NameCat#
H2AR3me1, biotinylated16-0359H3R8me2a, biotinylated 16-0380
H2AR3me2a, biotinylated 16-0360H3R8me2s, biotinylated 16-0381
H2AR3me2s, biotinylated16-0361H3R17me1, biotinylated 16-0382
H3R2me1, biotinylated16-0340H3R17me2a, biotinylated 16-0375
H3R2me2a, biotinylated16-0341H4R3me1, biotinylated 16-0356
H3R2me2s, biotinylated16-0355H4R3me2a, biotinylated16-0357
H3R8me1, biotinylated 16-0379H4R3me2s, biotinylated16-0358

Lysine Ubiquitination

NameCat#NameCat#
H2AUb1, biotinylated16-0020H2BK120ub, biotinylated16-0370
H2AK119ub, biotinylated16-0363H3K14ub, biotinylated16-0391
H2AK129ub, biotinylated16-0394

Other PTMs

NameCat#NameCat#
H3R2,8,17cit, biotinylated16-0362H3S10phos, non-biotinylated16-1345
H3R2,8,17cit, non-biotinylated16-1362H3.1S28phos, biotinylated16-0388
H3S10phos, biotinylated16-0345H3.3S31phos, biotinylated16-0389

Small Pack Sizes Available

NameCat#NameCat#
H3K4me1, biotinylated16-0321H3K9me3, biotinylated16-0315
H3K4me2, biotinylated16-0334H3K27ac, biotinylated16-0365
H3K4me2, non-biotinylated16-1334H3K27me3, biotinylated16-0317
H3K9ac, biotinylated16-0314H3K36me2, biotinylated16-0319
H3K9me2, biotinylated16-0324H3K36me3, biotinylated16-0320

vNucs Histone Variants

NameCat#
H2AX16-0013
H2AX, non-biotinylated16-1013
H2AXS139phos 16-0366
H2AZ.116-0014
H2AZ.1, non-biotinylated16-1014
H2AZ.216-0015
H3.316-0011
H3.3, non-biotinylated 100 μg16-0012

Methyl DNA Nucs Nucleosomes with Methylated DNA

NameCat#
Mononucleosomes, Hemi-methylated, Biotinylated16-2003
Mononucleosomes, Hemi-methylated16-2103
Mononucleosomes, 187×601 DNA, Biotinylated16-2004
Mononucleosomes, 187×601 DNA16-2104

SNAP-ChIP™ Spike-in Panels

NameCat#
SNAP-ChIP K-MetStat Panel19-1001
SNAP-ChIP OncoStat Panel19-2001
SNAP-ChIP K-AcylStat Panel19-3001

oncoNucs AA Subs. in Cancer

NameCat#
H3.3K4M16-0349
H3.3K9M16-0350
H3.3K27M16-1323
H3.3K27M, non-biotinylated16-0323
H3.3G34R16-0346
H3.3G34V16-0347
H3.3G34W16-0348
H3.3K36M16-0344

EpiDyne™ Chromatin Remodeling Assay Substrates

NameCat#
EpiDyne FRET Nucleosome Remodeling Assay Substrate16-4201
EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC116-4101
EpiDyne Remodeling Assay Substrate DNA ST601-GATC018-4100
EpiDyne Remodeling Assay Substrate DNA ST601-GATC118-4101

dCypher™ Nucleosome Panels

NameCat#
dCypher™ Nucleosome Full Panel16-9001
dCypher™ Nucleosome K-MetStat & OncoStat Panel16-9002
dCypher™ Nucleosome K-AcylStat Panel16-9003
dCypher™ Nucleosome R-MetStat Panel16-9004

SNAP-ChIP™ spike-in panels use modified DNA-barcoded Designer Nucleosomes (dNucs™) carrying disease-relevant histone PTMs as quantitative controls and for antibody validation, enabling unprecedented control of ChIP experiments.

Three ways SNAP-ChIP spike-ins improve ChIP

  • Identify and/or validate PTM-specific antibody
  • In situ monitoring of ChIP performance
  • Quantitative normalization
NameCat#
SNAP-ChIP™ K-MetStat PanelSKU: 19-1001
SNAP-ChIP™ K-AcylStat PanelSKU: 19-3001
SNAP-ChIP™ OncoStat PanelSKU: 19-2001
Histone H3K4me3 Antibody: SNAP-ChIP™ Certified, CUTANA™ CUT&RUN CompatibleSKU: 13-0041
Histone H3K27me3 Antibody, SNAP-ChIP™ Certified, CUTANA™ CUT&RUN CompatibleSKU: 13-0030
SNAP-ChIP™ Dual Labeled Hydrolysis ProbeSKU: 18-6001
SNAP-ChIP™ K-MetStat™ Full Panel Primer SetSKU: 18-6101
SNAP-ChIP™ OncoStat™ Panel Primer SetSKU: 18-6201
SNAP-ChIP™ K-MetStat™ H3K4 MiniPanel Primer SetSKU: 18-6102
SNAP-ChIP™ K-MetStat™ H3K9 MiniPanel Primer SetSKU: 18-6103
SNAP-ChIP™ K-MetStat™ H3K27 MiniPanel Primer SetSKU: 18-6104
SNAP-ChIP™ K-MetStat™ H3K36 MiniPanel Primer SetSKU: 18-6105
SNAP-ChIP™ K-MetStat™ H4K20 MiniPanel Primer SetSKU: 18-6106

EpiCypher dNucs™ are semi-synthetic recombinant nucleosomes, containing one (or more) histone PTMs. Nucleosomes are the natural target of chromatin readers and modifying enzymes, and thus provide a superior substrate for epigenetic enzymes and interactor proteins

dNuc substrates enable cutting-edge chromatin research
EpiCypher has an extensive library of >70 uniquely modified dNucs, useful for:

  • dCypher™: Discovery platform to study chromatin readers and enzymes
  • AlphaNuc™: HTS assays for inhibitor development
  • EpiDyne™: Quantitative chromatin remodeling assays

dCypher™ Nucleosome Panels provide unprecedented access to epigenetic diversity in a physiologically relevant nucleosome context. Each panels contain single and combinatorially-modified nucleosomes, and can be purchased as focused sets (Lysine Acylation, Arginine Methylation and the Lysine-Methylation / Oncostat panels) or as one large panel (Full Nucleosome Panel).

Three ways to leverage dCypher Nucleosome Panel diversity

Chromatin remodeling has been implicated in diverse diseases, including most types of cancer. The study of chromatin remodeling requires highly defined nucleosomal substrates, which are challenging to produce and adapt to various assays. The EpiDyne™ platform addresses this unmet need, leveraging EpiCypher dNucs™ as substrates for a variety of nucleosome remodeling assays.

EpiDyne substrates are available for three assay readouts

  • FRET – HTS compatible
  • Radiometric DAM methyltransferase assays – HTS compatible
  • Restriction enzyme accessibility
Recombinant Nucleosomes (rNucs) are human recombinant nucleosomes comprised of histone proteins made in E. coli wrapped with fully synthetic Widom 601 DNA. As the nucleosome is the true physiologic substrate for many histone modifying enzyme, these are excellent substrates for enzyme screening assays.
Nucleosomes: Nature’s preferred chromatin substrate
EpiCypher has an extensive library of recombinant nucleosomes, including
  • rNucs wrapped with hemi-methylated and methylated DNA templates
  • Unmodified rNucs, which can be used as controls in chromatin assays
  • rNucs assembled with histone variants (i.e. H2AZ)
NameCat#
Mononucleosomes, (H3.1ΔN32), Recombinant, 187×601 DNASKU: 16-2134
Mononucleosomes, Recombinant Human, DesthiobiotinylatedSKU: 16-0024
Tailless Nucleosomes, Recombinant Human, BiotinylatedSKU: 16-0027
Mononucleosomes, Recombinant HumanSKU: 16-0009
Mononucleosomes, Recombinant Human BiotinylatedSKU: 16-0006
Mononucleosomes, Recombinant, Hemi-methylated, BiotinylatedSKU: 16-2003
Mononucleosomes, Recombinant, Hemi-methylatedSKU: 16-2103
Mononucleosomes (H2AX), Human RecombinantSKU: 16-1013
Mononucleosomes (H2AX), Human Recombinant BiotinylatedSKU: 16-0013
Mononucleosomes (H2AZ.1), Human RecombinantSKU: 16-1014
Mononucleosomes (H2AZ.1), Human Recombinant BiotinylatedSKU: 16-0014
Mononucleosomes (H2AZ.2), Human Recombinant BiotinylatedSKU: 16-0015
Mononucleosomes (H3.3), Human Recombinant BiotinylatedSKU: 16-0011
Mononucleosomes (H3.3), Human RecombinantSKU: 16-0012
Mononucleosomes (H3.1 N32), Recombinant HumanSKU: 16-1016
Mononucleosomes (H3.1 ΔN32), Recombinant Human BiotinylatedSKU: 16-0016
Mononucleosomes (H3.3 N32), Recombinant Human BiotinylatedSKU: 16-0017
Mononucleosomes (H3.3 ΔN32), Recombinant HumanSKU: 16-1017
In addition to our extensive library of recombinant nucleosomes, EpiCypher offers highly purified nucleosomes obtained from either HeLa cells or avian erythrocytes, free from extraneous DNA and contaminating enzyme activities.
Purified nucleosomes : the ultimate experimental control
  • Use as a positive control in Western blot
  • Can be used as substrates for enzymatic reactions
  • Test binding activity of chromatin reader proteins
NameCat#
HeLa Mononucleosomes, PurifiedSKU: 16-0002
HeLa Polynucleosomes, PurifiedSKU: 16-0003
Chicken Mononucleosomes, PurifiedSKU: 16-0019
Chicken Polynucleosomes, PurifiedSKU: 16-0004
Chicken Polynucleosomes, Purified (HSW)SKU: 16-0022
Many scientists are interested in using specific parts of our recombinant nucleosomes, either for their own nucleosome reconstitutions or more custom experiments. EpiCypher™ is pleased to offer recombinant subunits of nucleosomes including histone octamers, tetramers, dimers and the Widom 601 sequence DNA.
The building blocks of nucleosomes
  • Have been used to assemble CENP-A variant nucleosomes for microscopy (Stumme-Diers et al. 2019)
  • Nucleosome binding assays (Chang et al. 2019)
  • Applied as controls in western blotting (Masuda et al. 2015)
NameCat#
Histone Octamer (H3.1 ΔN32), Recombinant HumanSKU: 16-8016
Histone Octamer (H4 ΔN15), Recombinant HumanSKU: 16-8018
Histone Octamer (H3.3), Human RecombinantSKU: 16-8012
Histone Octamer, Recombinant HumanSKU: 16-0001
Nucleosome Assembly 601 Sequence DNA, BiotinylatedSKU: 18-0005
EpiDyne Remodeling Assay Substrate DNA ST601-GATC0SKU: 18-4100
EpiDyne Remodeling Assay Substrate DNA ST601-GATC1SKU: 18-4101
SNAP-ChIP™ Dual Labeled Hydrolysis ProbeSKU: 18-6001
SNAP-ChIP™ K-MetStat™ Full Panel Primer SetSKU: 18-6101
SNAP-ChIP™ OncoStat™ Panel Primer SetSKU: 18-6201
SNAP-ChIP™ K-MetStat™ H3K4 MiniPanel Primer SetSKU: 18-6102
SNAP-ChIP™ K-MetStat™ H3K9 MiniPanel Primer SetSKU: 18-6103
SNAP-ChIP™ K-MetStat™ H3K27 MiniPanel Primer SetSKU: 18-6104
SNAP-ChIP™ K-MetStat™ H3K36 MiniPanel Primer SetSKU: 18-6105
SNAP-ChIP™ K-MetStat™ H4K20 MiniPanel Primer SetSKU: 18-6106
Histone H2A/H2B Dimer, Recombinant HumanSKU: 15-0311
Histone H3/H4 Tetramer, Recombinant HumanSKU: 16-0008
Histone variants have important roles across diverse cellular processes, including DNA replication, DNA repair, and transcriptional regulation. EpiCypher variant nucleosomes (vNucs) are assembled with defined histone variants, providing a physiological substrate for the functional analysis of these non-canonical nucleosomes.
Three ways to leverage vNucs for chromatin research
  • Use as substrates for testing for inhibitors
  • Study effects of variant histone on enzyme activity
  • Test specificity of antibody
NameCat#
Mononucleosome (H3.1ΔN2), Recombinant Human, BiotinylatedSKU: 16-0023
Mononucleosomes (H2AX), Human RecombinantSKU: 16-1013
Mononucleosomes (H2AX), Human Recombinant BiotinylatedSKU: 16-0013
Mononucleosomes (H2AZ.1), Human RecombinantSKU: 16-1014
Mononucleosomes (H2AZ.1), Human Recombinant BiotinylatedSKU: 16-0014
Mononucleosomes (H2AZ.2), Human Recombinant BiotinylatedSKU: 16-0015
Mononucleosomes (H3.3), Human Recombinant BiotinylatedSKU: 16-0011
Mononucleosomes (H3.3), Human RecombinantSKU: 16-0012

Somatic mutations in histone proteins occur in diverse cancer types, but have been challenging to study in a physiological context. EpiCypher’s oncogenic nucleosomes (oncoNucs) are assembled with histones containing mutations specifically associated with cancer, thus providing highly relevant nucleosomal substrates for cancer research.

Advancing cancer research with oncoNucs

  • Study effects of mutations on enzyme activity
  • Use as substrates for testing for drug / inhibitors
  • Suitable for high-throughput screening
NameCat#
Mononucleosomes (H3.3G34R), Recombinant Human, BiotinylatedSKU: 16-0346
Mononucleosomes (H3.3K4M), Recombinant Human BiotinylatedSKU: 16-0349
Mononucleosomes (H3.3K9M), Recombinant Human BiotinylatedSKU: 16-0350
Mononucleosomes (H3.3 K27M), Recombinant HumanSKU: 16-0323
Mononucleosomes (H3.3 K27M), Recombinant Human BiotinylatedSKU: 16-1323
Mononucleosomes (H3.3G34V), Recombinant Human BiotinylatedSKU: 16-0347
Mononucleosomes (H3.3G34W), Recombinant Human BiotinylatedSKU: 16-0348
Mononucleosomes (H3.3K36M), Recombinant Human BiotinylatedSKU: 16-0344

DNA methylation is one of the most well-studied epigenetic modifications, and has known roles in regulating gene expression and genomic stability. EpiCypher methyl DNA designer nucleosomes (MeDNA dNucs) are unmodified nucleosomes wrapped with unmethylated or hemi-methylated DNA, and are the optimal substrates for interrogating the function of DNA methylation on the binding activity of chromatin-associated proteins or inhibitors.

Three ways to use MeDNA dNucs to explore chromatin function

  • Study impact of MeDNA dNucs on chromatin modifying enzymes
  • Use MeDNA dNucs as substrates to identify methylation-sensitive inhibitors
  • Apply to high-throughput screening
NameCat#
Mononucleosomes, Recombinant, 187×601 DNASKU: 16-2104
Mononucleosomes, Recombinant, 187×601 DNA, BiotinylatedSKU: 16-2004
Mononucleosomes, Recombinant, Hemi-methylated, BiotinylatedSKU: 16-2003
Mononucleosomes, Recombinant, Hemi-methylatedSKU: 16-2103
Nucleosome Assembly 601 Sequence, 187bp, BiotinylatedSKU: 18-2004
Nucleosome Assembly 601 Sequence, 187bp, Hemi-methylated, BiotinylatedSKU: 18-2003

Antibodies

Poor specificity of histone PTM antibodies is a widespread problem in epigenetics research. EpiCypher SNAP-ChIP™ Certified Antibodies have been extensively validated using SNAP-ChIP spike-in technology, delivering PTM antibodies with the highest specificity and IP efficiency on the market.
Take the guesswork out of ChIP experiments
  • Validated using SNAP-ChIP spike-in controls
  • Low cross-reactivity; High IP efficiency
  • Extensive lot-specific testing of each antibody
  • The result? Best-in-class antibodies for optimized ChIP

CUT&RUN provides high-resolution chromatin profiling with low cell input and sequencing requirements. CUTANA™ CUT&RUN Compatible Antibodies have been rigorously tested for reliable and robust performance in CUT&RUN assays. Each antibody passes our stringent SNAP-ChIP™ certification criteria, and sequencing results between ChIP-seq and CUT&RUN are highly correlated.

Which antibodies work in CUT&RUN? Let EpiCypher do the work for you!

  • Each antibody passes our stringent SNAP-ChIP™ certification testing (high specificity and enrichment)
  • Results are highly correlated between ChIP-seq and CUT&RUN assays
  • Compatible with our recently released CUTANA CUT&RUN protocol
  • New antibodies being released – check back for updates!

EpiCypher offers a wide range of antibodies to help study lysine methylation, especially with regards to histone post-translational modifications (PTMs). Many of these are EpiCypher SNAP-ChIP™ Certified Antibodies, which have been extensively validated using SNAP-ChIP spike-in technology, delivering histone methylation-specific antibodies with the highest specificity and IP efficiency on the market.

Antibodies are a key reagent for chromatin research
Our antibodies can be used for:

  • ChIP-qPCR and ChIP-seq analysis
  • Capture or detection in ELISA and immunoblot
  • Immunostaining to determine localization of PTMs / nucleosomes
NameCat#
Histone H3 K4Me3 AntibodySKU: 13-0004
Histone H3K4me3 Antibody: SNAP-ChIP® Certified, CUTANA™ CUT&RUN CompatibleSKU: 13-0041
Histone H3 K9Me1 AntibodySKU: 13-0014
Histone H3K4me2 Antibody, SNAP-ChIP Certified (monoclonal)SKU: 13-0027
Histone H3K4me3 Antibody, SNAP-ChIP Certified SKU: 13-0028
Histone H3K9me1 Antibody, SNAP-ChIP Certified SKU: 13-0029
Histone H3K27me3 Antibody, SNAP-ChIP™ Certified, CUTANA™ CUT&RUN CompatibleSKU: 13-0030
Histone H3K36me3 Antibody, SNAP-ChIP™ Certified, CUTANA™ CUT&RUN CompatibleSKU: 13-0031
SETD2 AntibodySKU: 13-0025
SETD6 AntibodySKU: 13-0003

EpiCypher offers a wide range of antibodies to help study arginine methylation, including those specific for the enzymes that catalyze the modifications.

Antibodies are a key reagent for chromatin research
Our antibodies can be used for:

  • Capture or detection in ELISA and immunoblot
  • Immunostaining to determine localization of PTMs / nucleosomes
NameCat#
Asymmetric Dimethyl-arginine Antibody (ADMA) Asym26SKU: 13-0011
CARM1 / PRMT4 AntibodySKU: 13-0006
PRMT1 AntibodySKU: 13-0007
PRMT5 / JBP1 AntibodySKU: 13-0008
PRMT7 AntibodySKU: 13-0009
Symmetric Dimethyl-arginine Antibody (SDMA) Sym10SKU: 13-0012
Histone H3 R8Me1 AntibodySKU: 13-0017
Histone H3 R8Me2a AntibodySKU: 13-0018
Histone H3 R8Me2s AntibodySKU: 13-0019
Histone H3 R17Me2a AntibodySKU: 13-0016

GST Epitope Tag Antibody
SKU: 13-0022
Pack Size: 100 μg

Type:  Polyclonal
Host:  Rabbit
Mol Wgt.:  N/A
Appl.:  IF, WB
Format:  Serum
Reactivity:  N/A

GST Epitope Tag Antibody Description:
Glutathione S-transferase (GST) is a 26 kDa protein from Schistosoma japonica and is used as an epitope tag for improving the expression and solubility of GST fusion proteins expressed in E coli. This antibody is useful for detection of GST-tagged proteins on the EpiTitan Histone Peptide array and for detection of GST-tagged fusion proteins by Western Blot, including proteins bound to specific peptides in pull-down experiments.

Antibodies specific for a variety of enzymes that modify histones.

NameCat#
CARM1 / PRMT4 AntibodySKU: 13-0006
PRMT1 AntibodySKU: 13-0007
PRMT5 / JBP1 AntibodySKU: 13-0008
PRMT7 AntibodySKU: 13-0009
SETD2 AntibodySKU: 13-0025

EpiCypher offers a wide range of anti-nucleosome antibodies for various chromatin assays, targeting specific histone post-translational modifications, such as arginine methylation, and unmodified histones. In addition, our SNAP-ChIP™ Certified Antibodies have been extensively validated for ChIP experiments, using recombinant modified designer nucleosomes (dNucs) for in-application testing in a nucleosome context.

Antibodies are a key reagent for chromatin research
Our antibodies can be applied for:

  • ChIP-qPCR and ChIP-seq analysis
  • Capture or detection in ELISA and immunoblot
  • Immunostaining to determine localization of PTMs / nucleosomes

Epigenetics Reagents and Assays

CUTANA™ CUT&RUN reagents, kits, and assay services map histone PTMs and chromatin-interacting proteins with high resolution, at a fraction of the time and cost of standard ChIP-seq experiments.
CUTANA CUT&RUN assays offer clear advantages over ChIP
  • Reduced cell input : Compatible with as few as 5,000 cells
  • Diverse target profiling : histone PTMs and chromatin-interacting proteins (including remodelers)
  • Low background: Fewer required sequencing reads per sample (3-5 million)
  • Lower cost: Less required antibody and sequencing depth saves money
NameCat#
CUTANA ChIC/CUT&RUN Kit14-1048-EPC
CUTANA™ CUT&RUN 8-strip 0.2mL tubes10-0009-EPC
CUTANA™ E. coli Spike-in DNA18-1401-EPC
CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows - 50 rxns15-1016-EPC
CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody13-0042-EPC
Magnetic Separation Rack, 1.5 mL Tubes10-0012-EPC
CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows - 250 Rxns15-1116-EPC
Magnetic Separation Rack, 0.2 mL Tubes10-0008-EPC
CUTANA™ Concanavalin A Conjugated Paramagnetic Beads21-1401-EPC
Histone H3K4me3 Antibody: SNAP-ChIP™ Certified, CUTANA™ CUT&RUN Compatible13-0041-EPC
Histone H3K27me3 Antibody, SNAP-ChIP™ Certified, CUTANA™ CUT&RUN Compatible13-0030-EPC
Histone H3K36me3 Antibody, SNAP-ChIP™ Certified, CUTANA™ CUT&RUN Compatible13-0031-EPC

CUTANA™ CUT&Tag reagents, kits, and assay services enable rapid, cost-effective epigenomic mapping and deliver high quality data with improved resolution compared to ChIP-seq.

Try CUTANA CUT&Tag assays for next-generation epigenomic profiling

  • Streamlined 2-day protocol: from samples to NGS libraries in a single tube
  • Low background: Fewer required sequencing reads per sample (3-5 million)
  • Improved sensitivity: high quality data with ultra-low sample input
  • Lower cost: Less required antibody and sequencing depth saves money
NameCat#
CUTANA™ pAG-Tn5 for ChIC/CUT&Tag - 50 rxnsSKU: 15-1017
CUTANA™ pAG-Tn5 for ChIC/CUT&Tag - 250 RxnsSKU: 15-1117
CUTANA™ Concanavalin A Conjugated Paramagnetic BeadsSKU: 21-1401
Histone H3K4me3 Antibody: SNAP-ChIP™ Certified, CUTANA™ CUT&RUN CompatibleSKU: 13-0041
CUTANA™ Rabbit IgG CUT&RUN Negative Control AntibodySKU: 13-0042
Magnetic Separation Rack, 0.2 mL TubesSKU: 10-0008
Magnetic Separation Rack, 1.5 mL TubesSKU: 10-0012
CUTANA™ CUT&RUN 8-strip 0.2mL tubesSKU: 10-0009
CUTANA™ High Fidelity 2X PCR Master MixSKU: 15-1018
Histone H3K27me3 Antibody, SNAP-ChIP™ Certified, CUTANA™ CUT&RUN CompatibleSKU: 13-0030

Nuclear extracts can be a rich source of chromatin-associated proteins, including transcription factors, chromatin modifying enzymes, and regulatory complexes, and are commonly used as controls or to profile binding interactions of novel proteins. EpiCypher offers high-quality HeLa cell nuclear extracts for these diverse chromatin applications.

Three ways to apply HeLa Nuclear Extracts

  • Screening inhibitors
  • Substrates for enzymatic assays (e.g. HDACs)
  • Controls in immunoblotting

EpiCypher offers mammalian vectors encoding wild-type histone H3.3, as well as the oncogenic H3.3K9M and H3.3K27M. These histones can be used to screen binders or inhibitors that are sensitive to common histone mutations.

Recombinant Proteins

ATP-dependent chromatin remodeling enzymes are among the most commonly mutated proteins in cancer, making them a top target for the development of novel therapeutic strategies. The EpiDyne™ platform leverages EpiCypher dNucs as substrates to study nucleosome remodeling. To complement these assays, EpiCypher offers a range of remodeling enzymes, including SMARCA2 and SMARCA4 (the ATPases of the SWI/SNF complex) and the ACF ISWI-type complex.

EpiDyne : advanced tools for next-generation epigenetics assays
EpiDyne Chromatin Remodeling Enzymes can be used in a variety of assay readouts, including:

  • FRET – HTS compatible
  • Radiometric DAM methyltransferase assays – HTS compatible
  • Restriction enzyme accessibility
NameCat#
SMARCA2 Chromatin Remodeling Enzyme (Human BRM)SKU: 15-1015
SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)SKU: 15-1014
ACF Chromatin Remodeling Enzyme ComplexSKU: 15-1013

Recombinant histone proteins are the building block of nucleosomes, and an essential reagent in chromatin research. EpiCypher offers highly pure recombinant histone proteins, including canonical histones (H2A, H2B, H3 and H4) and histone variants, such as H2AZ.1, H2AZ.2 and H2AX.

Application of EpiCypher Recombinant Histones

  • Nucleosome binding assays (Chang et al. 2019)
  • Have been used to assemble CENP-A variant nucleosomes for microscopy (Stumme-Diers et al. 2019)
  • Applied as controls in western blotting (Masuda et al. 2015)
Chromatin architecture is composed of a diverse array of histone PTMs, which influence gene expression via the binding activity of various chromatin reader proteins. These proteins contain binding domains that target specific histone PTMs, and are important subjects in academic and pharmaceutical research. EpiCypher™ offers a wide range of recombinant GST-tagged histone binding domains, including chromodomains and bromodomains, which can be used in a variety of downstream applications.
EpiCypher histone PTM binding domains
  • Bromodomain Proteins : Histone Acetylation
  • YEATs Proteins : Histone Acylation
  • Chromodomain Proteins : Histone Methylation
  • PHD domain proteins : Histone Methylation
  • MBT domain proteins : Histone Methylation
HP1ß, Recombinant Human, His-Tagged
SKU: 15-0074
Type:  Chromodomain
Host:  E. coli
Mol Wgt.:  24.4 kDa
Epitope Tag:  6xHis
HP1ß, Recombinant Human, His-Tagged Description: Recombinant human HP1ß (CBX1 M31, Heterochromatin protein 1 beta, accession number P83916, amino acids 1 to 184) containing an N-terminal 6xHis tag, expressed in E. coli. HP1ß is a component of constitutive (transcriptionally silent) heterochromatin, recruited through the interaction of the chromodomain with histone H3 methylated at lysine 9 (H3K9me3).
HP1ß, Recombinant Human, His-Tagged Formulation: Recombinant 6xHis-tagged protein in 20 mM Tris-HCl pH 7.5, 100 mM NaCl, 1 mM DTT, 5% glycerol.
HP1ß, Recombinant Human, His-Tagged Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
HP1ß, Recombinant Human, His-Tagged Application Notes: HP1ß, Recombinant Human, His-Tagged is useful for protein binding and screening experiments examining methylated protein and peptide substrates.
References: Li Y et al (2014)

Chromatin modifying enzymes target specific histone residues for post-translational modification, including methylation, acetylation, phosphorylation. As such, these proteins drive major changes in chromatin structure, and are important targets in academic and pharmaceutical research. EpiCypher offers a library of histone methyltransferase enzymes and catalytic domains, each highly pure and validated for on-target substrates.

Applications for chromatin modifying enzymes

  • Inhibitor assays
  • Methyltransferase assays
NameCat#
SMARCA2 Chromatin Remodeling Enzyme (Human BRM)SKU: 15-1015
SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)SKU: 15-1014
ACF Chromatin Remodeling Enzyme ComplexSKU: 15-1013
5x ACF Remodeling Assay BufferSKU: 21-0013
DOT1L Catalytic Domain, Recombinant HumanSKU: 15-1001
G9a, Recombinant Human, His-taggedSKU: 15-1010
NSD2 / MMSET Catalytic Domain, Recombinant HumanSKU: 15-1002
SETD2, Recombinant HumanSKU: 15-1012
SETD2, Recombinant Human GST-taggedSKU: 15-1011
SETD6, Recombinant HumanSKU: 15-1004
SMYD2, Recombinant HumanSKU: 15-1006
SMYD3, Recombinant HumanSKU: 15-1007

Histone Peptides and Arrays

EpiCypher histone peptide arrays enable rapid, high-throughput screening of chromatin effector proteins, antibodies, and enzymes against a diverse repertoire of single and combinatorial histone PTMs. We offer two versions of histone peptide arrays : the EpiTitan™ array (95 unique modifications) and the EpiTriton™ array (296 unique modifications). Extensive validation and purification of peptides is performed prior to histone peptide array printing to ensure the highest quality product for your studies.

Advantages of EpiCypher Histone Peptide Arrays

  • Significantly reduced false-negatives and false positives, reducing time and cost of experiments
  • Can Perform multiple experiments on a single array; each sub-array contains 4 replicates of each peptide
  • HPLC quality control of all peptides allows full confidence in peptide consistency from array analysis to follow-on experiments
  • Are not limited to 20 residues, as all peptides are synthesized and validated prior to array spotting
  • Up to 6 unique modifications per peptide allows for greater understanding and utility of peptide protein interaction analysis
NameCat#
NEW EpiTriton™ Histone Peptide ArraySKU: 11-4001

EpiCypher modified histone peptides are highly pure and extensively validated, ensuring the highest quality product for chromatin research. Our modified H2A peptides are biotinylated, allowing use in peptide pull down and / or binding assays.

Advantages of EpiCypher Histone Peptides

  • 99% pure by MALDI-TOF mass spectrometry and HPLC analysis
  • Available as unmodified and modified varieties, including combinatorial PTMs
EpiCypher modified histone peptides are highly pure and extensively validated, ensuring the highest quality product for chromatin research. Our modified H2B peptides are biotinylated, allowing use in peptide pull down and / or binding assays.
Advantages of EpiCypher Histone Peptides
  • 99% pure by MALDI-TOF mass spectrometry and HPLC analysis
  • Available as unmodified and modified varieties, including combinatorial PTMs
NameCat#
Histone H2B N-terminal Peptide, BiotinylatedSKU: 12-0077
Histone H2BK12, K15, K16, K20ac Peptide, BiotinylatedSKU: 12-0211
Histone H2BK12ac Peptide, BiotinylatedSKU: 12-0207
Histone H2BK15ac Peptide, BiotinylatedSKU: 12-0208
Histone H2BK16ac Peptide, BiotinylatedSKU: 12-0209
Histone H2BK20ac Peptide, BiotinylatedSKU: 12-0210
Histone H2BK5me1 Peptide, BiotinylatedSKU: 12-0206
Histone H2BK5me2 Peptide, BiotinylatedSKU: 12-0205
Histone H2BK5me3 Peptide, BiotinylatedSKU: 12-0204

EpiCypher modified histone peptides are highly pure and extensively validated, ensuring the highest quality product for chromatin research. Our modified H3 peptides are biotinylated, allowing use in peptide pull down and / or binding assays.

Advantages of EpiCypher Histone Peptides

  • 99% pure by MALDI-TOF mass spectrometry and HPLC analysis
  • Available as unmodified and modified varieties, including combinatorial PTMs

EpiCypher modified histone peptides are highly pure and extensively validated, ensuring the highest quality product for chromatin research. Our modified H4 peptides are biotinylated, allowing use in peptide pull down and / or binding assays.

Advantages of EpiCypher Histone Peptides

  • 99% pure by MALDI-TOF mass spectrometry and HPLC analysis
  • Available as unmodified and modified varieties, including combinatorial PTMs

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Exploring Chromatin Remodeling Substrates with Nucleosomes

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Peptide or Nucleosome?

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Designer nucleosomes important for epigenetics research

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