EpiCypher offers cutting edge tools and service for epigenetics and chromatin biology research. We pioneered the manufacture of designer recombinant nucleosomes that contain defined post-translational modifications. Our latest product, SNAP-ChIP, is the gold standard for antibody validation for chromatin IP, and also serves as the most reproducible spike-in for normalizing ChIP and ChIP-seq assays. EpiCypher also develops assays for chromatin-modifying enzymes and PTM-binding proteins using a variety of readouts, such as Alpha, FRET and radioactivity, employing nucleosome or peptide-based substrates.


Product Categories

Featured Technologies

EpiCypher is dedicated to creating novel platforms that support innovative chromatin research. We are constantly expanding our product lines to provide the latest in quantitative chromatin technology.

New CUTANA CUNT&RUN
Compatible Antibodies

Extensively validated. Meticulously optimized.

NEW! CUTANA™
ChIC / CUT&RUN Kit

Extensively validated. Meticulously optimized.

CUTANA™ CUT&Tag Assays

Ultra-sensitive genomic profiling assays with lower input and sequencing read depth requirements than ChIP

SNAP-ChIP™ spike-ins

Accurate ChIP-seq antibody validation and data normalization using DNA-barcoded nucleosome spike-ins

CUTANA™ ChIC / CUT&RUN Assays

Robust, high-resolution chromatin profiling, at a fraction of the time and cost of ChIP-seq

Nucleosomes

rNucs Human Recombinant Nucleosomes, No PTMs

Name Cat. No.
Mononucleosomes, biotinylated 16-0006
Mononucleosomes, non-biotinylated 16-0009
Mononucleosomes, desthiobiotinylated 16-0024
Tailless Nucleosomes, biotinylated 16-0027
Mononucleosomes, H3.1 ΔN2, biotinylated 16-0023
Mononucleosomes, H3.1 ΔN32, biotinylated 16-0016
Mononucleosomes, H3.1 ΔN32, non-biotinylated 16-1016
Mononucleosomes, H3.3 ΔN32, biotinylated 16-0017
Mononucleosomes, H3.3 ΔN32, non-biotinylated 16-0017
Mononucleosomes, H4 ΔN15, biotinylated 16-0018

dNucs Designer Recombinant Nucleosomes with PTMs

Lysine Methylation

Name Cat# Name Cat#
H3K4me1, biotinylated 16-0321 H3K36me1, biotinylated 16-0322
H3K4me2, biotinylated 16-0334 H3K36me2, biotinylated 16-0319
H3K4me2, non-biotinylated 16-1334 H3K36me2, non-biotinylated 16-1319
H3K4me3, biotinylated 16-0316 H3K36me3, biotinylated 16-0320
H3K4me3, non-biotinylated 16-1316 H3K36me3, non-biotinylated 16-1320
H3K4me3,K9,14,18ac 16-0335 H3.3K36me3, biotinylated 16-0390
H3K9me1, biotinylated 16-0325 H3K79me1, biotinylated 16-0367
H3K9me2, biotinylated 16-0324 H3K79me2, biotinylated 16-0368
H3K9me3, biotinylated 16-0315 H3K79me3, biotinylated 16-0369
H3K27me1, biotinylated 16-0338 H4K12me1, biotinylated 16-0393
H3K27me2, biotinylated 16-0339 H4K20me1, biotinylated 16-0331
H3K27me3, biotinylated 16-0317 H4K20me2, biotinylated 16-0332
H3K27me3, non-biotinylated 16-1317 H4K20me3, biotinylated 16-0333
H4K20me3, non-biotinylated 16-1333

Lysine Acylation

Name Cat# Name Cat#
H2AK5,9,13,15ac, biotinylated 16-0376 H3K27bu, biotinylated 16-0384
H2BK12ac, biotinylated 16-0386 H3K27cr, biotinylated 16-0383
H2BK20ac, biotinylated 16-0387 H3K27ac, S28phos, biotinylated 16-0385
H3K4ac, biotinylated 16-0342 H3K36ac, biotinylated 16-0378
H3K9ac, biotinylated 16-0314 H4K5ac, biotinylated 16-0352
H3K9ac, non-biotinylated 16-1314 H4K8ac, biotinylated 16-0353
H3K9bu, biotinylated 16-0371 H4K12ac, biotinylated 16-0312
H3K9cr, biotinylated 16-0351 H4K16ac, biotinylated 16-0354
H3K14ac, biotinylated 16-0343 H4K20ac, biotinylated 16-0377
H3K18ac, biotinylated 16-0372 H2AK5,9,13,15ac, biotinylated 16-0376
H3K18bu, biotinylated 16-0373 H3K4,9,14,18ac, biotinylated 16-0336
H3K18cr, biotinylated 16-0337 H3K4me3, K9,14,18ac, biotinylated 16-0335
H3K23ac, biotinylated 16-0364 H4K5,8,12,16ac, biotinylated 16-0313
H3K27ac, biotinylated 16-0365 H4K5,8,12,16ac, non-biotinylated 16-1313
H3K27ac, non-biotinylated 16-1365 H3K4,9,14,18ac / H4K5,8,12,16ac, biotinylated 16-0374
H3K27acS28phos, biotinylated 16-0385

 

Arginine Methylation

Name Cat# Name Cat#
H2AR3me1, biotinylated 16-0359 H3R8me2a, biotinylated 16-0380
H2AR3me2a, biotinylated 16-0360 H3R8me2s, biotinylated 16-0381
H2AR3me2s, biotinylated 16-0361 H3R17me1, biotinylated 16-0382
H3R2me1, biotinylated 16-0340 H3R17me2a, biotinylated 16-0375
H3R2me2a, biotinylated 16-0341 H4R3me1, biotinylated 16-0356
H3R2me2s, biotinylated 16-0355 H4R3me2a, biotinylated 16-0357
H3R8me1, biotinylated 16-0379 H4R3me2s, biotinylated 16-0358

 

Lysine Ubiquitination

Name Cat# Name Cat#
H2AUb1, biotinylated 16-0020 H2BK120ub, biotinylated 16-0370
H2AK119ub, biotinylated 16-0363 H3K14ub, biotinylated 16-0391
H2AK129ub, biotinylated 16-0394

 

Other PTMs

Name Cat# Name Cat#
H3R2,8,17cit, biotinylated 16-0362 H3S10phos, non-biotinylated 16-1345
H3R2,8,17cit, non-biotinylated 16-1362 H3.1S28phos, biotinylated 16-0388
H3S10phos, biotinylated 16-0345 H3.3S31phos, biotinylated 16-0389

 

Small Pack Sizes Available

Name Cat# Name Cat#
H3K4me1, biotinylated 16-0321 H3K9me3, biotinylated 16-0315
H3K4me2, biotinylated 16-0334 H3K27ac, biotinylated 16-0365
H3K4me2, non-biotinylated 16-1334 H3K27me3, biotinylated 16-0317
H3K9ac, biotinylated 16-0314 H3K36me2, biotinylated 16-0319
H3K9me2, biotinylated 16-0324 H3K36me3, biotinylated 16-0320

 

vNucs Histone Variants

Name Cat#
H2AX 16-0013
H2AX, non-biotinylated 16-1013
H2AXS139phos 16-0366
H2AZ.1 16-0014
H2AZ.1, non-biotinylated 16-1014
H2AZ.2 16-0015
H3.3 16-0011
H3.3, non-biotinylated 100 μg 16-0012

Methyl DNA Nucs Nucleosomes with Methylated DNA

Name Cat#
Mononucleosomes, Hemi-methylated, Biotinylated 16-2003
Mononucleosomes, Hemi-methylated 16-2103
Mononucleosomes, 187×601 DNA, Biotinylated 16-2004
Mononucleosomes, 187×601 DNA 16-2104

SNAP-ChIP™ Spike-in Panels

Name Cat#
SNAP-ChIP K-MetStat Panel 19-1001
SNAP-ChIP OncoStat Panel 19-2001
SNAP-ChIP K-AcylStat Panel 19-3001

oncoNucs AA Subs. in Cancer

Name Cat#
H3.3K4M 16-0349
H3.3K9M 16-0350
H3.3K27M 16-1323
H3.3K27M, non-biotinylated 16-0323
H3.3G34R 16-0346
H3.3G34V 16-0347
H3.3G34W 16-0348
H3.3K36M 16-0344

EpiDyne™ Chromatin Remodeling Assay Substrates

Name Cat#
EpiDyne FRET Nucleosome Remodeling Assay Substrate 16-4201
EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1 16-4101
EpiDyne Remodeling Assay Substrate DNA ST601-GATC0 18-4100
EpiDyne Remodeling Assay Substrate DNA ST601-GATC1 18-4101

dCypher™ Nucleosome Panels

Name Cat#
dCypher™ Nucleosome Full Panel 16-9001
dCypher™ Nucleosome K-MetStat & OncoStat Panel 16-9002
dCypher™ Nucleosome K-AcylStat Panel 16-9003
dCypher™ Nucleosome R-MetStat Panel 16-9004

SNAP-ChIP™ spike-in panels use modified DNA-barcoded Designer Nucleosomes (dNucs™) carrying disease-relevant histone PTMs as quantitative controls and for antibody validation, enabling unprecedented control of ChIP experiments.

Three ways SNAP-ChIP spike-ins improve ChIP

  • Identify and/or validate PTM-specific antibody
  • In situ monitoring of ChIP performance
  • Quantitative normalization

 

Name Cat#
SNAP-ChIP™ K-MetStat Panel SKU: 19-1001
SNAP-ChIP™ K-AcylStat Panel SKU: 19-3001
SNAP-ChIP™ OncoStat Panel SKU: 19-2001
Histone H3K4me3 Antibody: SNAP-ChIP™ Certified, CUTANA™ CUT&RUN Compatible SKU: 13-0041
Histone H3K27me3 Antibody, SNAP-ChIP™ Certified, CUTANA™ CUT&RUN Compatible SKU: 13-0030
SNAP-ChIP™ Dual Labeled Hydrolysis Probe SKU: 18-6001
SNAP-ChIP™ K-MetStat™ Full Panel Primer Set SKU: 18-6101
SNAP-ChIP™ OncoStat™ Panel Primer Set SKU: 18-6201
SNAP-ChIP™ K-MetStat™ H3K4 MiniPanel Primer Set SKU: 18-6102
SNAP-ChIP™ K-MetStat™ H3K9 MiniPanel Primer Set SKU: 18-6103
SNAP-ChIP™ K-MetStat™ H3K27 MiniPanel Primer Set SKU: 18-6104
SNAP-ChIP™ K-MetStat™ H3K36 MiniPanel Primer Set SKU: 18-6105
SNAP-ChIP™ K-MetStat™ H4K20 MiniPanel Primer Set SKU: 18-6106

EpiCypher dNucs™ are semi-synthetic recombinant nucleosomes, containing one (or more) histone PTMs. Nucleosomes are the natural target of chromatin readers and modifying enzymes, and thus provide a superior substrate for epigenetic enzymes and interactor proteins

dNuc substrates enable cutting-edge chromatin research
EpiCypher has an extensive library of >70 uniquely modified dNucs, useful for:

  • dCypher™: Discovery platform to study chromatin readers and enzymes
  • AlphaNuc™: HTS assays for inhibitor development
  • EpiDyne™: Quantitative chromatin remodeling assays

dCypher™ Nucleosome Panels provide unprecedented access to epigenetic diversity in a physiologically relevant nucleosome context. Each panels contain single and combinatorially-modified nucleosomes, and can be purchased as focused sets (Lysine Acylation, Arginine Methylation and the Lysine-Methylation / Oncostat panels) or as one large panel (Full Nucleosome Panel).

Three ways to leverage dCypher Nucleosome Panel diversity

Chromatin remodeling has been implicated in diverse diseases, including most types of cancer. The study of chromatin remodeling requires highly defined nucleosomal substrates, which are challenging to produce and adapt to various assays. The EpiDyne™ platform addresses this unmet need, leveraging EpiCypher dNucs™ as substrates for a variety of nucleosome remodeling assays.

EpiDyne substrates are available for three assay readouts

  • FRET – HTS compatible
  • Radiometric DAM methyltransferase assays – HTS compatible
  • Restriction enzyme accessibility
Recombinant Nucleosomes (rNucs) are human recombinant nucleosomes comprised of histone proteins made in E. coli wrapped with fully synthetic Widom 601 DNA. As the nucleosome is the true physiologic substrate for many histone modifying enzyme, these are excellent substrates for enzyme screening assays.
Nucleosomes: Nature’s preferred chromatin substrate
EpiCypher has an extensive library of recombinant nucleosomes, including
  • rNucs wrapped with hemi-methylated and methylated DNA templates
  • Unmodified rNucs, which can be used as controls in chromatin assays
  • rNucs assembled with histone variants (i.e. H2AZ)

 

Name Cat#
Mononucleosomes, (H3.1ΔN32), Recombinant, 187×601 DNA SKU: 16-2134
Mononucleosomes, Recombinant Human, Desthiobiotinylated SKU: 16-0024
Tailless Nucleosomes, Recombinant Human, Biotinylated SKU: 16-0027
Mononucleosomes, Recombinant Human SKU: 16-0009
Mononucleosomes, Recombinant Human Biotinylated SKU: 16-0006
Mononucleosomes, Recombinant, Hemi-methylated, Biotinylated SKU: 16-2003
Mononucleosomes, Recombinant, Hemi-methylated SKU: 16-2103
Mononucleosomes (H2AX), Human Recombinant SKU: 16-1013
Mononucleosomes (H2AX), Human Recombinant Biotinylated SKU: 16-0013
Mononucleosomes (H2AZ.1), Human Recombinant SKU: 16-1014
Mononucleosomes (H2AZ.1), Human Recombinant Biotinylated SKU: 16-0014
Mononucleosomes (H2AZ.2), Human Recombinant Biotinylated SKU: 16-0015
Mononucleosomes (H3.3), Human Recombinant Biotinylated SKU: 16-0011
Mononucleosomes (H3.3), Human Recombinant SKU: 16-0012
Mononucleosomes (H3.1 N32), Recombinant Human SKU: 16-1016
Mononucleosomes (H3.1 ΔN32), Recombinant Human Biotinylated SKU: 16-0016
Mononucleosomes (H3.3 N32), Recombinant Human Biotinylated SKU: 16-0017
Mononucleosomes (H3.3 ΔN32), Recombinant Human SKU: 16-1017
In addition to our extensive library of recombinant nucleosomes, EpiCypher offers highly purified nucleosomes obtained from either HeLa cells or avian erythrocytes, free from extraneous DNA and contaminating enzyme activities.
Purified nucleosomes : the ultimate experimental control
  • Use as a positive control in Western blot
  • Can be used as substrates for enzymatic reactions
  • Test binding activity of chromatin reader proteins

 

Name Cat#
HeLa Mononucleosomes, Purified SKU: 16-0002
HeLa Polynucleosomes, Purified SKU: 16-0003
Chicken Mononucleosomes, Purified SKU: 16-0019
Chicken Polynucleosomes, Purified SKU: 16-0004
Chicken Polynucleosomes, Purified (HSW) SKU: 16-0022
Many scientists are interested in using specific parts of our recombinant nucleosomes, either for their own nucleosome reconstitutions or more custom experiments. EpiCypher™ is pleased to offer recombinant subunits of nucleosomes including histone octamers, tetramers, dimers and the Widom 601 sequence DNA.
The building blocks of nucleosomes
  • Have been used to assemble CENP-A variant nucleosomes for microscopy (Stumme-Diers et al. 2019)
  • Nucleosome binding assays (Chang et al. 2019)
  • Applied as controls in western blotting (Masuda et al. 2015)

 

Name Cat#
Histone Octamer (H3.1 ΔN32), Recombinant Human SKU: 16-8016
Histone Octamer (H4 ΔN15), Recombinant Human SKU: 16-8018
Histone Octamer (H3.3), Human Recombinant SKU: 16-8012
Histone Octamer, Recombinant Human SKU: 16-0001
Nucleosome Assembly 601 Sequence DNA, Biotinylated SKU: 18-0005
EpiDyne Remodeling Assay Substrate DNA ST601-GATC0 SKU: 18-4100
EpiDyne Remodeling Assay Substrate DNA ST601-GATC1 SKU: 18-4101
SNAP-ChIP™ Dual Labeled Hydrolysis Probe SKU: 18-6001
SNAP-ChIP™ K-MetStat™ Full Panel Primer Set SKU: 18-6101
SNAP-ChIP™ OncoStat™ Panel Primer Set SKU: 18-6201
SNAP-ChIP™ K-MetStat™ H3K4 MiniPanel Primer Set SKU: 18-6102
SNAP-ChIP™ K-MetStat™ H3K9 MiniPanel Primer Set SKU: 18-6103
SNAP-ChIP™ K-MetStat™ H3K27 MiniPanel Primer Set SKU: 18-6104
SNAP-ChIP™ K-MetStat™ H3K36 MiniPanel Primer Set SKU: 18-6105
SNAP-ChIP™ K-MetStat™ H4K20 MiniPanel Primer Set SKU: 18-6106
Histone H2A/H2B Dimer, Recombinant Human SKU: 15-0311
Histone H3/H4 Tetramer, Recombinant Human SKU: 16-0008
Histone variants have important roles across diverse cellular processes, including DNA replication, DNA repair, and transcriptional regulation. EpiCypher variant nucleosomes (vNucs) are assembled with defined histone variants, providing a physiological substrate for the functional analysis of these non-canonical nucleosomes.
Three ways to leverage vNucs for chromatin research
  • Use as substrates for testing for inhibitors
  • Study effects of variant histone on enzyme activity
  • Test specificity of antibody

 

Name Cat#
Mononucleosome (H3.1ΔN2), Recombinant Human, Biotinylated SKU: 16-0023
Mononucleosomes (H2AX), Human Recombinant SKU: 16-1013
Mononucleosomes (H2AX), Human Recombinant Biotinylated SKU: 16-0013
Mononucleosomes (H2AZ.1), Human Recombinant SKU: 16-1014
Mononucleosomes (H2AZ.1), Human Recombinant Biotinylated SKU: 16-0014
Mononucleosomes (H2AZ.2), Human Recombinant Biotinylated SKU: 16-0015
Mononucleosomes (H3.3), Human Recombinant Biotinylated SKU: 16-0011
Mononucleosomes (H3.3), Human Recombinant SKU: 16-0012

Somatic mutations in histone proteins occur in diverse cancer types, but have been challenging to study in a physiological context. EpiCypher’s oncogenic nucleosomes (oncoNucs) are assembled with histones containing mutations specifically associated with cancer, thus providing highly relevant nucleosomal substrates for cancer research.

Advancing cancer research with oncoNucs

  • Study effects of mutations on enzyme activity
  • Use as substrates for testing for drug / inhibitors
  • Suitable for high-throughput screening

 

Name Cat#
Mononucleosomes (H3.3G34R), Recombinant Human, Biotinylated SKU: 16-0346
Mononucleosomes (H3.3K4M), Recombinant Human Biotinylated SKU: 16-0349
Mononucleosomes (H3.3K9M), Recombinant Human Biotinylated SKU: 16-0350
Mononucleosomes (H3.3 K27M), Recombinant Human SKU: 16-0323
Mononucleosomes (H3.3 K27M), Recombinant Human Biotinylated SKU: 16-1323
Mononucleosomes (H3.3G34V), Recombinant Human Biotinylated SKU: 16-0347
Mononucleosomes (H3.3G34W), Recombinant Human Biotinylated SKU: 16-0348
Mononucleosomes (H3.3K36M), Recombinant Human Biotinylated SKU: 16-0344

DNA methylation is one of the most well-studied epigenetic modifications, and has known roles in regulating gene expression and genomic stability. EpiCypher methyl DNA designer nucleosomes (MeDNA dNucs) are unmodified nucleosomes wrapped with unmethylated or hemi-methylated DNA, and are the optimal substrates for interrogating the function of DNA methylation on the binding activity of chromatin-associated proteins or inhibitors.

Three ways to use MeDNA dNucs to explore chromatin function

  • Study impact of MeDNA dNucs on chromatin modifying enzymes
  • Use MeDNA dNucs as substrates to identify methylation-sensitive inhibitors
  • Apply to high-throughput screening

 

Name Cat#
Mononucleosomes, Recombinant, 187×601 DNA SKU: 16-2104
Mononucleosomes, Recombinant, 187×601 DNA, Biotinylated SKU: 16-2004
Mononucleosomes, Recombinant, Hemi-methylated, Biotinylated SKU: 16-2003
Mononucleosomes, Recombinant, Hemi-methylated SKU: 16-2103
Nucleosome Assembly 601 Sequence, 187bp, Biotinylated SKU: 18-2004
Nucleosome Assembly 601 Sequence, 187bp, Hemi-methylated, Biotinylated SKU: 18-2003

Histone mutations cause distinct changes in nucleosome structure, chromatin accessibility and gene expression, and are linked to numerous disease states. This research has also revealed the importance of considering the entire nucleosome structure in chromatin interactions, as several of these mutations occur within the nucleosome core and modulate PTM crosstalk and multivalent binding mechanisms. EpiCypher offers a unique collection of Mutant Nucleosomes (Mutant Nucs) providing defined substrates for binding assays, drug research, and more.

Substrate Types and Applications
  • Oncogenic Nucleosomes (e.g. H3.3K27M, H3.3G34R)
  • Acidic Patch Mutant Nucleosomes (e.g. H2AE61A, H2AE92K)
  • Study impact of mutant histones on protein binding / enzymatic function
  • Useful for drug / inhibitor development and HTS

Acidic Patch Nucleosomes

The nucleosome acidic patch refers to a small set of negatively charged amino acids clustered on the surface of the nucleosome disk, at the interface of histones H2A and H2B. This patch serves as a central binding site for many chromatin binding proteins, including nucleosome remodeling complexes, and has emerged as a key regulator of multivalent chromatin interactions. The acidic patch has also been shown to modulate high-order chromatin folding and condensation through extensive interactions with histone tails. EpiCypher Acidic Patch Mutant Nucs have defined mutations in the acidic patch, and are useful substrates for biochemical studies, drug development, and more.

Ideal Substrates for Functional Studies
  • Three unique Acidic Patch Mutant Nucs available
  • Define multivalent interactions involving the acidic patch
  • Useful for drug / inhibitor development and HTS
  • Valuable controls for structural analysis

Oncogenic Nucleosomes (oncoNucs)

Mutations in histone proteins occur in diverse cancer types but have been challenging to study in a physiological context. A recent large-scale study of more than 180 tumor types suggests that somatic histone mutations occur in 4% of cancers, highlighting novel epigenetic diversity that may be leveraged for future drug and biomarker development. EpiCypher’s Oncogenic Nucleosomes, or oncoNucs, are assembled using histones with cancer-associated mutations, providing highly relevant nucleosome substrates for cancer research.

Advancing cancer research with oncoNucs
  • Study effects of mutations on enzyme activity and chromatin reader binding
  • Use as substrates for drug / inhibitor testing and HTS
  • Physiological controls for characterizing chromatin interactions

 

Product Name Product Code
Nucleosome, Recombinant Human, Acidic Patch Mutant H2BE105A,E113A  16-1031
Nucleosome, Recombinant Human, Acidic Patch Mutant H2AE92K  16-1030
Nucleosome, Recombinant Human, Acidic Patch Mutant H2AE61A  16-1029
Nucleosome, Recombinant Human, Acidic Patch Mutant H2AE61A Biotinylated  16-0029
Nucleosome, Recombinant Human, Acidic Patch Mutant H2AE92K Biotinylated  16-0030
Nucleosome, Recombinant Human, Acidic Patch Mutant H2BE105A,E113A Biotinylated  16-0031
Mononucleosomes (H3.3G34R), Recombinant Human, Biotinylated  16-0346
Mononucleosomes (H3.3K4M), Recombinant Human Biotinylated  16-0349
Mononucleosomes (H3.3K9M), Recombinant Human Biotinylated  16-0350
Mononucleosomes (H3.3 K27M), Recombinant Human  16-0323
Mononucleosomes (H3.3 K27M), Recombinant Human Biotinylated  16-1323
Mononucleosomes (H3.3G34V), Recombinant Human Biotinylated  16-0347
Mononucleosomes (H3.3G34W), Recombinant Human Biotinylated  16-0348
Mononucleosomes (H3.3K36M), Recombinant Human Biotinylated  16-0344

Antibodies

Poor specificity of histone PTM antibodies is a widespread problem in epigenetics research. EpiCypher SNAP-ChIP™ Certified Antibodies have been extensively validated using SNAP-ChIP spike-in technology, delivering PTM antibodies with the highest specificity and IP efficiency on the market.
Take the guesswork out of ChIP experiments
  • Validated using SNAP-ChIP spike-in controls
  • Low cross-reactivity; High IP efficiency
  • Extensive lot-specific testing of each antibody
  • The result? Best-in-class antibodies for optimized ChIP

CUT&RUN provides high-resolution chromatin profiling with low cell input and sequencing requirements. CUTANA™ CUT&RUN Compatible Antibodies have been rigorously tested for reliable and robust performance in CUT&RUN assays. Each antibody passes our stringent SNAP-ChIP™ certification criteria, and sequencing results between ChIP-seq and CUT&RUN are highly correlated.

Which antibodies work in CUT&RUN? Let EpiCypher do the work for you!

  • Each antibody passes our stringent SNAP-ChIP™ certification testing (high specificity and enrichment)
  • Results are highly correlated between ChIP-seq and CUT&RUN assays
  • Compatible with our recently released CUTANA CUT&RUN protocol
  • New antibodies being released – check back for updates!

EpiCypher offers a wide range of antibodies to help study lysine methylation, especially with regards to histone post-translational modifications (PTMs). Many of these are EpiCypher SNAP-ChIP™ Certified Antibodies, which have been extensively validated using SNAP-ChIP spike-in technology, delivering histone methylation-specific antibodies with the highest specificity and IP efficiency on the market.

Antibodies are a key reagent for chromatin research
Our antibodies can be used for:

  • ChIP-qPCR and ChIP-seq analysis
  • Capture or detection in ELISA and immunoblot
  • Immunostaining to determine localization of PTMs / nucleosomes

 

Name Cat#
Histone H3 K4Me3 Antibody SKU: 13-0004
Histone H3K4me3 Antibody: SNAP-ChIP® Certified, CUTANA™ CUT&RUN Compatible SKU: 13-0041
Histone H3 K9Me1 Antibody SKU: 13-0014
Histone H3K4me2 Antibody, SNAP-ChIP Certified (monoclonal) SKU: 13-0027
Histone H3K4me3 Antibody, SNAP-ChIP Certified SKU: 13-0028
Histone H3K9me1 Antibody, SNAP-ChIP Certified SKU: 13-0029
Histone H3K27me3 Antibody, SNAP-ChIP™ Certified, CUTANA™ CUT&RUN Compatible SKU: 13-0030
Histone H3K36me3 Antibody, SNAP-ChIP™ Certified, CUTANA™ CUT&RUN Compatible SKU: 13-0031
SETD2 Antibody SKU: 13-0025
SETD6 Antibody SKU: 13-0003

EpiCypher offers a wide range of antibodies to help study arginine methylation, including those specific for the enzymes that catalyze the modifications.

Antibodies are a key reagent for chromatin research
Our antibodies can be used for:

  • Capture or detection in ELISA and immunoblot
  • Immunostaining to determine localization of PTMs / nucleosomes

 

Name Cat#
Asymmetric Dimethyl-arginine Antibody (ADMA) Asym26 SKU: 13-0011
CARM1 / PRMT4 Antibody SKU: 13-0006
PRMT1 Antibody SKU: 13-0007
PRMT5 / JBP1 Antibody SKU: 13-0008
PRMT7 Antibody SKU: 13-0009
Symmetric Dimethyl-arginine Antibody (SDMA) Sym10 SKU: 13-0012
Histone H3 R8Me1 Antibody SKU: 13-0017
Histone H3 R8Me2a Antibody SKU: 13-0018
Histone H3 R8Me2s Antibody SKU: 13-0019
Histone H3 R17Me2a Antibody SKU: 13-0016

GST Epitope Tag Antibody
SKU: 13-0022
Pack Size: 100 μg

Type:  Polyclonal
Host:  Rabbit
Mol Wgt.:  N/A
Appl.:  IF, WB
Format:  Serum
Reactivity:  N/A

GST Epitope Tag Antibody Description:
Glutathione S-transferase (GST) is a 26 kDa protein from Schistosoma japonica and is used as an epitope tag for improving the expression and solubility of GST fusion proteins expressed in E coli. This antibody is useful for detection of GST-tagged proteins on the EpiTitan Histone Peptide array and for detection of GST-tagged fusion proteins by Western Blot, including proteins bound to specific peptides in pull-down experiments.

Antibodies specific for a variety of enzymes that modify histones.

 

Name Cat#
CARM1 / PRMT4 Antibody SKU: 13-0006
PRMT1 Antibody SKU: 13-0007
PRMT5 / JBP1 Antibody SKU: 13-0008
PRMT7 Antibody SKU: 13-0009
SETD2 Antibody SKU: 13-0025

EpiCypher offers a wide range of anti-nucleosome antibodies for various chromatin assays, targeting specific histone post-translational modifications, such as arginine methylation, and unmodified histones. In addition, our SNAP-ChIP™ Certified Antibodies have been extensively validated for ChIP experiments, using recombinant modified designer nucleosomes (dNucs) for in-application testing in a nucleosome context.

Antibodies are a key reagent for chromatin research
Our antibodies can be applied for:

  • ChIP-qPCR and ChIP-seq analysis
  • Capture or detection in ELISA and immunoblot
  • Immunostaining to determine localization of PTMs / nucleosomes

Epigenetics Reagents and Assays

CUTANA™ CUT&RUN reagents, kits, and assay services map histone PTMs and chromatin-interacting proteins with high resolution, at a fraction of the time and cost of standard ChIP-seq experiments.
CUTANA CUT&RUN assays offer clear advantages over ChIP
  • Reduced cell input : Compatible with as few as 5,000 cells
  • Diverse target profiling : histone PTMs and chromatin-interacting proteins (including remodelers)
  • Low background: Fewer required sequencing reads per sample (3-5 million)
  • Lower cost: Less required antibody and sequencing depth saves money

 

Name Cat#
CUTANA ChIC/CUT&RUN Kit 14-1048-EPC
CUTANA™ CUT&RUN 8-strip 0.2mL tubes 10-0009-EPC
CUTANA™ E. coli Spike-in DNA 18-1401-EPC
CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows – 50 rxns 15-1016-EPC
CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody 13-0042-EPC
Magnetic Separation Rack, 1.5 mL Tubes 10-0012-EPC
CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows – 250 Rxns 15-1116-EPC
Magnetic Separation Rack, 0.2 mL Tubes 10-0008-EPC
CUTANA™ Concanavalin A Conjugated Paramagnetic Beads 21-1401-EPC
Histone H3K4me3 Antibody: SNAP-ChIP™ Certified, CUTANA™ CUT&RUN Compatible 13-0041-EPC
Histone H3K27me3 Antibody, SNAP-ChIP™ Certified, CUTANA™ CUT&RUN Compatible 13-0030-EPC
Histone H3K36me3 Antibody, SNAP-ChIP™ Certified, CUTANA™ CUT&RUN Compatible 13-0031-EPC

CUTANA™ CUT&Tag reagents, kits, and assay services enable rapid, cost-effective epigenomic mapping and deliver high quality data with improved resolution compared to ChIP-seq.

Try CUTANA CUT&Tag assays for next-generation epigenomic profiling

  • Streamlined 2-day protocol: from samples to NGS libraries in a single tube
  • Low background: Fewer required sequencing reads per sample (3-5 million)
  • Improved sensitivity: high quality data with ultra-low sample input
  • Lower cost: Less required antibody and sequencing depth saves money

 

Name Cat#
CUTANA™ pAG-Tn5 for ChIC/CUT&Tag – 50 rxns SKU: 15-1017
CUTANA™ pAG-Tn5 for ChIC/CUT&Tag – 250 Rxns SKU: 15-1117
CUTANA™ Concanavalin A Conjugated Paramagnetic Beads SKU: 21-1401
Histone H3K4me3 Antibody: SNAP-ChIP™ Certified, CUTANA™ CUT&RUN Compatible SKU: 13-0041
CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody SKU: 13-0042
Magnetic Separation Rack, 0.2 mL Tubes SKU: 10-0008
Magnetic Separation Rack, 1.5 mL Tubes SKU: 10-0012
CUTANA™ CUT&RUN 8-strip 0.2mL tubes SKU: 10-0009
CUTANA™ High Fidelity 2X PCR Master Mix SKU: 15-1018
Histone H3K27me3 Antibody, SNAP-ChIP™ Certified, CUTANA™ CUT&RUN Compatible SKU: 13-0030

Nuclear extracts can be a rich source of chromatin-associated proteins, including transcription factors, chromatin modifying enzymes, and regulatory complexes, and are commonly used as controls or to profile binding interactions of novel proteins. EpiCypher offers high-quality HeLa cell nuclear extracts for these diverse chromatin applications.

Three ways to apply HeLa Nuclear Extracts

  • Screening inhibitors
  • Substrates for enzymatic assays (e.g. HDACs)
  • Controls in immunoblotting

EpiCypher offers mammalian vectors encoding wild-type histone H3.3, as well as the oncogenic H3.3K9M and H3.3K27M. These histones can be used to screen binders or inhibitors that are sensitive to common histone mutations.

Recombinant Proteins

ATP-dependent chromatin remodeling enzymes are among the most commonly mutated proteins in cancer, making them a top target for the development of novel therapeutic strategies. The EpiDyne™ platform leverages EpiCypher dNucs as substrates to study nucleosome remodeling. To complement these assays, EpiCypher offers a range of remodeling enzymes, including SMARCA2 and SMARCA4 (the ATPases of the SWI/SNF complex) and the ACF ISWI-type complex.

EpiDyne : advanced tools for next-generation epigenetics assays
EpiDyne Chromatin Remodeling Enzymes can be used in a variety of assay readouts, including:

  • FRET – HTS compatible
  • Radiometric DAM methyltransferase assays – HTS compatible
  • Restriction enzyme accessibility

 

Name Cat#
SMARCA2 Chromatin Remodeling Enzyme (Human BRM) SKU: 15-1015
SMARCA4 Chromatin Remodeling Enzyme (Human BRG1) SKU: 15-1014
ACF Chromatin Remodeling Enzyme Complex SKU: 15-1013

Recombinant histone proteins are the building block of nucleosomes, and an essential reagent in chromatin research. EpiCypher offers highly pure recombinant histone proteins, including canonical histones (H2A, H2B, H3 and H4) and histone variants, such as H2AZ.1, H2AZ.2 and H2AX.

Application of EpiCypher Recombinant Histones

  • Nucleosome binding assays (Chang et al. 2019)
  • Have been used to assemble CENP-A variant nucleosomes for microscopy (Stumme-Diers et al. 2019)
  • Applied as controls in western blotting (Masuda et al. 2015)
Chromatin architecture is composed of a diverse array of histone PTMs, which influence gene expression via the binding activity of various chromatin reader proteins. These proteins contain binding domains that target specific histone PTMs, and are important subjects in academic and pharmaceutical research. EpiCypher™ offers a wide range of recombinant GST-tagged histone binding domains, including chromodomains and bromodomains, which can be used in a variety of downstream applications.
EpiCypher histone PTM binding domains
  • Bromodomain Proteins : Histone Acetylation
  • YEATs Proteins : Histone Acylation
  • Chromodomain Proteins : Histone Methylation
  • PHD domain proteins : Histone Methylation
  • MBT domain proteins : Histone Methylation
HP1ß, Recombinant Human, His-Tagged
SKU: 15-0074
Type:  Chromodomain
Host:  E. coli
Mol Wgt.:  24.4 kDa
Epitope Tag:  6xHis
HP1ß, Recombinant Human, His-Tagged Description: Recombinant human HP1ß (CBX1 M31, Heterochromatin protein 1 beta, accession number P83916, amino acids 1 to 184) containing an N-terminal 6xHis tag, expressed in E. coli. HP1ß is a component of constitutive (transcriptionally silent) heterochromatin, recruited through the interaction of the chromodomain with histone H3 methylated at lysine 9 (H3K9me3).
HP1ß, Recombinant Human, His-Tagged Formulation: Recombinant 6xHis-tagged protein in 20 mM Tris-HCl pH 7.5, 100 mM NaCl, 1 mM DTT, 5% glycerol.
HP1ß, Recombinant Human, His-Tagged Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
HP1ß, Recombinant Human, His-Tagged Application Notes: HP1ß, Recombinant Human, His-Tagged is useful for protein binding and screening experiments examining methylated protein and peptide substrates.
References: Li Y et al (2014)

Chromatin modifying enzymes target specific histone residues for post-translational modification, including methylation, acetylation, phosphorylation. As such, these proteins drive major changes in chromatin structure, and are important targets in academic and pharmaceutical research. EpiCypher offers a library of histone methyltransferase enzymes and catalytic domains, each highly pure and validated for on-target substrates.

Applications for chromatin modifying enzymes

  • Inhibitor assays
  • Methyltransferase assays

 

Name Cat#
SMARCA2 Chromatin Remodeling Enzyme (Human BRM) SKU: 15-1015
SMARCA4 Chromatin Remodeling Enzyme (Human BRG1) SKU: 15-1014
ACF Chromatin Remodeling Enzyme Complex SKU: 15-1013
5x ACF Remodeling Assay Buffer SKU: 21-0013
DOT1L Catalytic Domain, Recombinant Human SKU: 15-1001
G9a, Recombinant Human, His-tagged SKU: 15-1010
NSD2 / MMSET Catalytic Domain, Recombinant Human SKU: 15-1002
SETD2, Recombinant Human SKU: 15-1012
SETD2, Recombinant Human GST-tagged SKU: 15-1011
SETD6, Recombinant Human SKU: 15-1004
SMYD2, Recombinant Human SKU: 15-1006
SMYD3, Recombinant Human SKU: 15-1007

Histone Peptides and Arrays

EpiCypher histone peptide arrays enable rapid, high-throughput screening of chromatin effector proteins, antibodies, and enzymes against a diverse repertoire of single and combinatorial histone PTMs. We offer two versions of histone peptide arrays : the EpiTitan™ array (95 unique modifications) and the EpiTriton™ array (296 unique modifications). Extensive validation and purification of peptides is performed prior to histone peptide array printing to ensure the highest quality product for your studies.

Advantages of EpiCypher Histone Peptide Arrays

  • Significantly reduced false-negatives and false positives, reducing time and cost of experiments
  • Can Perform multiple experiments on a single array; each sub-array contains 4 replicates of each peptide
  • HPLC quality control of all peptides allows full confidence in peptide consistency from array analysis to follow-on experiments
  • Are not limited to 20 residues, as all peptides are synthesized and validated prior to array spotting
  • Up to 6 unique modifications per peptide allows for greater understanding and utility of peptide protein interaction analysis

 

Name Cat#
NEW EpiTriton™ Histone Peptide Array SKU: 11-4001

EpiCypher modified histone peptides are highly pure and extensively validated, ensuring the highest quality product for chromatin research. Our modified H2A peptides are biotinylated, allowing use in peptide pull down and / or binding assays.

Advantages of EpiCypher Histone Peptides

  • 99% pure by MALDI-TOF mass spectrometry and HPLC analysis
  • Available as unmodified and modified varieties, including combinatorial PTMs
EpiCypher modified histone peptides are highly pure and extensively validated, ensuring the highest quality product for chromatin research. Our modified H2B peptides are biotinylated, allowing use in peptide pull down and / or binding assays.
Advantages of EpiCypher Histone Peptides
  • 99% pure by MALDI-TOF mass spectrometry and HPLC analysis
  • Available as unmodified and modified varieties, including combinatorial PTMs

 

Name Cat#
Histone H2B N-terminal Peptide, Biotinylated SKU: 12-0077
Histone H2BK12, K15, K16, K20ac Peptide, Biotinylated SKU: 12-0211
Histone H2BK12ac Peptide, Biotinylated SKU: 12-0207
Histone H2BK15ac Peptide, Biotinylated SKU: 12-0208
Histone H2BK16ac Peptide, Biotinylated SKU: 12-0209
Histone H2BK20ac Peptide, Biotinylated SKU: 12-0210
Histone H2BK5me1 Peptide, Biotinylated SKU: 12-0206
Histone H2BK5me2 Peptide, Biotinylated SKU: 12-0205
Histone H2BK5me3 Peptide, Biotinylated SKU: 12-0204

EpiCypher modified histone peptides are highly pure and extensively validated, ensuring the highest quality product for chromatin research. Our modified H3 peptides are biotinylated, allowing use in peptide pull down and / or binding assays.

Advantages of EpiCypher Histone Peptides

  • 99% pure by MALDI-TOF mass spectrometry and HPLC analysis
  • Available as unmodified and modified varieties, including combinatorial PTMs

EpiCypher modified histone peptides are highly pure and extensively validated, ensuring the highest quality product for chromatin research. Our modified H4 peptides are biotinylated, allowing use in peptide pull down and / or binding assays.

Advantages of EpiCypher Histone Peptides

  • 99% pure by MALDI-TOF mass spectrometry and HPLC analysis
  • Available as unmodified and modified varieties, including combinatorial PTMs

SNAP-ChIP® Antibody Validation

SNAP-ChIP® antibody validation services identify high-performance ChIP antibodies with superior specificity and enrichment, all within the context of a ChIP experiment.

EpiDyne® Chromatin Remodeling HTS Assays

EpiDyne® enables direct detection of chromatin remodeling activity, and is ideal for HTS and inhibitor profiling. EpiDyne services accelerate assay development and generate robust results.

dCypher™ Assay Services for Novel Chromatin Interactions

dCypher™ assay services rapidly interrogate chromatin reader binding against a collection of modified histone peptides or nucleosomes, revealing novel mechanisms in chromatin biology.

Custom Nucleosome Development

Nucleosomes are the preferred target of chromatin interacting proteins, and are optimal substrates for epigenetics research. To address this need, EpiCypher provides custom nucleosome development.

EpiCypher provides SNAP-ChIP® antibody validation services for histone PTM-specific antibodies. SNAP-ChIP® spike-in controls are composed of DNA-barcoded recombinant designer nucleosomes (dNucs), and are only available from EpiCypher. These spike-ins can easily be added to any ChIP workflow to rigorously validate antibody specificity when it matters: in your experiment. Use EpiCypher’s SNAP-ChIP® antibody validation services to identify high performance ChIP-grade antibodies and generate quality ChIP-seq data you can trust.

What are the advantages of SNAP-ChIP® antibody validation services?

SNAP-ChIP is unique from other forms of ChIP antibody validation, such as modified histone peptide arrays, as it examines both the specificity and enrichment of a histone PTM antibody against a physiological nucleosome substrate during a standard ChIP workflow.

EpiCypher scientists have significant expertise in optimizing SNAP-ChIP for profiling antibodies to various histone PTM targets, including methylation, acetylation, crotonylation, butyrylation, and more. SNAP-ChIP services are ideal for:

  • Scientists new to SNAP-ChIP or chromatin profiling technologies
  • Projects that require extensive antibody screening and validation
  • Identification of an antibody to an understudied histone PTM

What are the service applications?

We offer SNAP-ChIP antibody validation services to identify highly specific antibodies for ChIP experiments. However, we also provide nucleosome-based antibody validation services for other applications:

  • AlphaLISA / AlphaScreen: For high-throughput biochemical and cell-based assays
  • ELISA: For biochemical or cell-based assays
  • And other customized assays as needed

Publications and Technical Notes

We recently used SNAP-ChIP to profile antibodies to H3K4 methyl states, revealing widespread cross-reactivity of these antibodies in ChIP:

For more information on how SNAP-ChIP experiments are performed, see the manual for the K-MetSata SNAP-CHIP panel.

Other Resources

To learn more about SNAP-ChIP technology, check out these additional resources:

Chromatin remodeling regulates DNA access on chromatin, thus impacting gene expression and genome repair. Many ATP-dependent chromatin remodeling enzymes are associated with human disease, but are challenging targets to study biochemically due to the requirement for nucleosome-based substrates. EpiCypher has addressed this need by developing EpiDyne® substrates, a family of highly pure recombinant nucleosomes designed to monitor chromatin remodeling activity in vitro. EpiDyne® assays enhance our understanding of chromatin remodeling mechanisms, revealing new avenues for targeted epigenetic therapy.

What are the advantages of EpiDyne® Chromatin Remodeling HTS Assay Services?

EpiDyne assays directly measure the effects of chromatin remodeling enzymes on a physiological nucleosome substrate. This is in direct contrast to leading assays, which examine chromatin remodeling with indirect methods, and have low sensitivity. With EpiDyne assay services you will have access to:

  • Expert assay development and optimization
  • Ultra-sensitive inhibitor profiling and specificity testing
  • HTS assay optimization and miniaturization

What are the service applications?

EpiDyne assays are compatible with multiple downstream formats:

  • FRET
  • Restriction Enzyme Accessibility
  • DAM methyltransferase

Technical Notes

EpiCypher has developed robust protocols for EpiDyne chromatin remodeling assays, including methods for restriction enzyme accessibility and FRET assays.

Resources

Need more information on EpiDyne? Here are some additional resources:

dCypher™ assay services combine our comprehensive library of recombinant nucleosomes and/or modified histone peptides with the high sensitivity of AlphaScreen® to discover novel chromatin interactions. This transformative technology identifies chromatin interactions that are missed by standard peptide arrays, while also requiring less starting material. As part of our high-throughput dCypher™ services, chromatin interacting proteins are screened against hundreds of histone PTMs in a single experiment, revealing the true binding specificity of chromatin readers, enzymes, antibodies, and more.

What are the advantages of dCypher™ assay services?

EpiCypher has considerable experience in developing and optimizing chromatin binding assays, including those for chromatin readers, writer / eraser enzymes, antibodies, and more. dCypher assay services leverage this expertise, combined with our comprehensive library of modified nucleosome and histone peptide substrates, to deliver highly sensitive and customized assays. There are multiple advantages to using dCypher assay services:

  • Detect chromatin interactions missed by histone peptide arrays
  • Screen against physiologically relevant targets, with access to >80 modified nucleosome substrates
  • Assess the binding of histone binding multiple domains in one experiment
  • Reduced protein input requirements (average is nM)
  • Accommodates full length proteins
  • Prior knowledge of histone PTM interactions not necessary
  • Have option of profiling against our library of ~300 modified histone peptides, including single and combinatorial histone PTMs

What are the service applications?

applications for dCyhper assay services include:

  • Drug target identification and validation
  • Reveal novel biological mechanisms
  • Determine how modifications impact chromatin enzyme activity
  • Examine chromatin reader binding specificity

Publications and Technical Notes

dCypher is starting to make a significant impact in epigenetics research. Learn about our most recent collaboration, in which dCypher was instrumental in revealing mechanism of DNMT3A recruitment at intergenic regions

EpiCypher has also published a series of Technical Notes to help scientists use dCypher assays in their chromatin research.

Resources

Looking for additional information on dCypher? Click through to learn more!

Nucleosomes are the in vivo targets of chromatin regulating proteins (i.e. readers, writers, and erasers), making them ideal biochemical substrates for drug development, and enable access to historically challenging targets. EpiCypher offers custom nucleosome development services, generating fully defined and homogeneous recombinant nucleosomes that incorporate different DNA and histone modifications, site mutations, or histone variants. We have developed a suite of robust technologies for these projects, allowing us to manufacture nucleosomes for nearly any downstream application.

What are the service applications?

EpiCypher nucleosomes can be used for a variety of downstream experiments, such as:

  • Study effects of combinations of epigenetic marks on enzyme activity or chromatin binding
  • Examine the biological properties of understudied PTMs
  • Suitable for enzyme assays, inhibitor testing, and high-throughput screening
  • Perform protein-protein interaction studies involving the modification of interest
  • Study impact of histone mutations or DNA methylation on enzyme activity / chromatin binding

What are the advantages of EpiCypher's custom nucleosome development services?

EpiCypher is an expert in the manufacturing of highly pure recombinant nucleosomes. With custom nucleosome services, you will have access to our impressive portfolio of nucleosome assembly technologies, including recombinant designer nucleosomes (dNucs™), EpiDyne® chromatin remodeling substrates, oncogenic nucleosomes (oncoNucs), and much more. In addition, all of our nucleosomes are subject to rigorous quality control, including validation by HPLC and high resolution mass spectrometry, meaning that you can be confident in your data.

With EpiCypher custom nucleosome development services you will have access to:

  • Assay development and optimization
  • HTS assay optimization and miniaturization
  • Assembly of nucleosomes with diverse DNA and histone modifications, site mutations and histone variants

versaNuc™ custom nucleosome development

versaNuc is our pilot platform for custom nucleosome development, enabling assembly of personalized panels of nucleosomes with unique histone H3 modifications and custom DNA templates (e.g. various DNA length, sequence, and methylations). versaNuc can be used to:

  • Create custom single nucleosomes or panels of nucleosomes
  • Define unique biological properties encoded by combinations of epigenetic marks
  • Develop physiological reagents to study poorly understood epigenetic changes

Technical Notes and Publications

Resources

Need more information on EpiCypher recombinant nucleosome technology? Check out these resources to learn more!


The Nucleosome Acidic Patch: A Master Landing Dock for Chromatin Regulators

The nucleosome is the repeating unit of chromatin and serves as the physiological substrate for diverse chromatin interacting proteins, including Read More...

Starting CUT&RUN for a new target? Don’t forget these key optimization steps

CUT&RUN is an ultra-sensitive chromatin mapping technology that is gaining widespread popularity in the epigenomics field. This streamlined approach is Read More...

Choosing the Right ChIP Antibody for Your Experiment

There are multiple components to designing and executing a successful chromatin immunoprecipitation (ChIP) or ChIP-seq experiment: - What target should Read More...

CUT&RUN and CUT&Tag Growth Outpacing ATAC-seq

CUT&RUN and CUT&Tag Growth Outpacing ATAC-seq

Cleavage Under Targets and Release Using Nuclease (CUT&RUN) is an innovative chromatin mapping strategy, which leverages breakthroughs from the Chromatin Read More...

Which is better for ChIP antibody validation?

Histone Peptides vs. Nucleosomes

Highly specific antibodies are a crucial component of epigenetics research, and form the foundation of multiple chromatin profiling assays. For Read More...

EpiCypher’s H3K4me3 Antibody Provides a Useful Positive Control for CUT&RUN

H3K4me3 Antibody

Cleavage Under Targets and Release Using Nuclease (CUT&RUN) assays are one of the latest advancements in chromatin profiling technologies, allowing Read More...

CUTANA-compatible antibodies

New CUTANA-compatible antibodies pave the way to study transcription factors and chromatin-interacting proteins with CUT&RUN EpiCypher recently launched CUTANA™ CUT&RUN assays, which Read More...

CUTANA™ ChIC / CUT&RUN Kit

Extensively validated. Meticulously optimized.View Product Cleavage Under Targets and Release using Nuclease (CUT&RUN), based on the previous Chromatin ImmunoCleavage (ChIC) Read More...

Nucleosomes

Nucleosome | Stratech What is a nucleosome  Nucleosomes are integral components of all eukaryotic genomes with the purpose of organising Read More...

CUTANA™ ChIC / CUT&RUN Assays

Reliable and robust chromatin mapping assays CUTANA™ ChIC / CUT&RUN assays leverage recent advancements in immunotethering technologies to deliver efficient, Read More...

SNAP-ChIP™ Spike-In Controls

Nucleosome spike-ins for Quantitative ChIP-Seq Great Experiments Require Great Controls Chromatin immunoprecipitation (ChIP) is a powerful and widely used approach Read More...

CUTANA™ CUT&Tag Assays

Ultra low input chromatin mapping assays CUTANA™ CUT&Tag assays build on recent advancements in immunotethering technology to deliver an innovative Read More...

dCypher™ Nucleosome Panels: Unparalleled Diversity for Rapid Histone PTM Profiling

Epigenetics is one of the fastest-growing areas of academic and pharmaceutical research, with many projects focused on understanding the functions Read More...

SNAP-ChIP™ Spike-In Controls are Essential for ChIP Experiments

Despite the overwhelming popularity of chromatin immunoprecipitation (ChIP) in epigenetics research, ChIP methods have poor yields, high background, and low Read More...

Exploring Chromatin Remodeling Substrates with Nucleosomes

Nucleosomes are the fundamental repeating units of chromatin, comprised of a histone octamer wrapped by ~147bp DNA. A common focus Read More...

Peptide or Nucleosome?

dCypher ™ Reveals the Optimal Substrate for Histone PTM Analyses Histone ‘reader’ proteins contain evolutionarily conserved domains that bind to Read More...

Designer nucleosomes important for epigenetics research

Nucleosome substrates are emerging tools for the in vitro study of chromatin epigenetic regulation, with applications from basic chromatin biology Read More...