Lysine Methylation

Lysine Acylation

Arginine Methylation

Lysine Ubiquitination

Other PTMs

Small Pack Sizes Available

SNAP-ChIP™ spike-in panels use modified DNA-barcoded Designer Nucleosomes (dNucs™) carrying disease-relevant histone PTMs as quantitative controls and for antibody validation, enabling unprecedented control of ChIP experiments.

Three ways SNAP-ChIP spike-ins improve ChIP

• Identify and/or validate PTM-specific antibody
• In situ monitoring of ChIP performance
• Quantitative normalization

SNAP CUTANA™ Spike-in Controls for CUT&RUN and CUT&Tag are panels of modified DNA-barcoded Designer Nucleosomes (dNucs™) carrying an array of widely studied histone post-translational modifications (PTMs). These controls offer a simple and cost-effective means to monitor assay success, antibody specificity and enable quantitative cross-sample comparisons to provide high confidence in your results.

EpiCypher dNucs™ are semi-synthetic recombinant nucleosomes, containing one (or more) histone PTMs. Nucleosomes are the natural target of chromatin readers and modifying enzymes, and thus provide a superior substrate for epigenetic enzymes and interactor proteins

dNuc substrates enable cutting-edge chromatin research
EpiCypher has an extensive library of >70 uniquely modified dNucs, useful for:

• dCypher™: Discovery platform to study chromatin readers and enzymes
• AlphaNuc™: HTS assays for inhibitor development
• EpiDyne™: Quantitative chromatin remodeling assays

dCypher™ Nucleosome Panels provide unprecedented access to epigenetic diversity in a physiologically relevant nucleosome context. Each panels contain single and combinatorially-modified nucleosomes, and can be purchased as focused sets (Lysine Acylation, Arginine Methylation and the Lysine-Methylation / Oncostat panels) or as one large panel (Full Nucleosome Panel).

Three ways to leverage dCypher Nucleosome Panel diversity

• Interrogating chromatin reader binding specificity: see the latest application of dCypher technology in Nature!
• Screening antibody specificity / efficiency
• As controls in IP, immunoblot, and ELISA

Chromatin remodeling has been implicated in diverse diseases, including most types of cancer. The study of chromatin remodeling requires highly defined nucleosomal substrates, which are challenging to produce and adapt to various assays. The EpiDyne™ platform addresses this unmet need, leveraging EpiCypher dNucs™ as substrates for a variety of nucleosome remodeling assays.

EpiDyne substrates are available for three assay readouts

• FRET – HTS compatible
• Radiometric DAM methyltransferase assays – HTS compatible
• Restriction enzyme accessibility

• rNucs wrapped with hemi-methylated and methylated DNA templates
• Unmodified rNucs, which can be used as controls in chromatin assays
• rNucs assembled with histone variants (i.e. H2AZ)

• Use as a positive control in Western blot
• Can be used as substrates for enzymatic reactions
• Test binding activity of chromatin reader proteins

• Have been used to assemble CENP-A variant nucleosomes for microscopy (Stumme-Diers et al. 2019)
• Nucleosome binding assays (Chang et al. 2019)
• Applied as controls in western blotting (Masuda et al. 2015)

• Use as substrates for testing for inhibitors
• Study effects of variant histone on enzyme activity
• Test specificity of antibody

Somatic mutations in histone proteins occur in diverse cancer types, but have been challenging to study in a physiological context. EpiCypher’s oncogenic nucleosomes (oncoNucs) are assembled with histones containing mutations specifically associated with cancer, thus providing highly relevant nucleosomal substrates for cancer research.

Advancing cancer research with oncoNucs

• Study effects of mutations on enzyme activity
• Use as substrates for testing for drug / inhibitors
• Suitable for high-throughput screening

DNA methylation is one of the most well-studied epigenetic modifications, and has known roles in regulating gene expression and genomic stability. EpiCypher methyl DNA designer nucleosomes (MeDNA dNucs) are unmodified nucleosomes wrapped with unmethylated or hemi-methylated DNA, and are the optimal substrates for interrogating the function of DNA methylation on the binding activity of chromatin-associated proteins or inhibitors.

Three ways to use MeDNA dNucs to explore chromatin function

• Study impact of MeDNA dNucs on chromatin modifying enzymes
• Use MeDNA dNucs as substrates to identify methylation-sensitive inhibitors
• Apply to high-throughput screening

Histone mutations cause distinct changes in nucleosome structure, chromatin accessibility and gene expression, and are linked to numerous disease states. This research has also revealed the importance of considering the entire nucleosome structure in chromatin interactions, as several of these mutations occur within the nucleosome core and modulate PTM crosstalk and multivalent binding mechanisms. EpiCypher offers a unique collection of Mutant Nucleosomes (Mutant Nucs) providing defined substrates for binding assays, drug research, and more.

Substrate Types and Applications

• Oncogenic Nucleosomes (e.g. H3.3K27M, H3.3G34R)
• Acidic Patch Mutant Nucleosomes (e.g. H2AE61A, H2AE92K)
• Study impact of mutant histones on protein binding / enzymatic function
• Useful for drug / inhibitor development and HTS

Acidic Patch Nucleosomes

Oncogenic Nucleosomes (oncoNucs)

• Validated using SNAP-ChIP spike-in controls
• Low cross-reactivity; High IP efficiency
• Extensive lot-specific testing of each antibody
• The result? Best-in-class antibodies for optimized ChIP

CUT&RUN provides high-resolution chromatin profiling with low cell input and sequencing requirements. CUTANA™ CUT&RUN Compatible Antibodies have been rigorously tested for reliable and robust performance in CUT&RUN assays. Each antibody passes our stringent SNAP-ChIP™ certification criteria, and sequencing results between ChIP-seq and CUT&RUN are highly correlated.

Which antibodies work in CUT&RUN? Let EpiCypher do the work for you!

• Each antibody passes our stringent SNAP-ChIP™ certification testing (high specificity and enrichment)
• Results are highly correlated between ChIP-seq and CUT&RUN assays
• Compatible with our recently released CUTANA CUT&RUN protocol
• New antibodies being released – check back for updates!

EpiCypher offers a wide range of antibodies to help study lysine methylation, especially with regards to histone post-translational modifications (PTMs). Many of these are EpiCypher SNAP-ChIP™ Certified Antibodies, which have been extensively validated using SNAP-ChIP spike-in technology, delivering histone methylation-specific antibodies with the highest specificity and IP efficiency on the market.

Antibodies are a key reagent for chromatin research
Our antibodies can be used for:

• ChIP-qPCR and ChIP-seq analysis
• Capture or detection in ELISA and immunoblot
• Immunostaining to determine localization of PTMs / nucleosomes

EpiCypher offers a wide range of antibodies to help study arginine methylation, including those specific for the enzymes that catalyze the modifications.

Antibodies are a key reagent for chromatin research
Our antibodies can be used for:

• Capture or detection in ELISA and immunoblot
• Immunostaining to determine localization of PTMs / nucleosomes

GST Epitope Tag Antibody
SKU: 13-0022
Pack Size: 100 μg

Type:  Polyclonal
Host:  Rabbit
Mol Wgt.:  N/A
Appl.:  IF, WB
Format:  Serum
Reactivity:  N/A

GST Epitope Tag Antibody Description:
Glutathione S-transferase (GST) is a 26 kDa protein from Schistosoma japonica and is used as an epitope tag for improving the expression and solubility of GST fusion proteins expressed in E coli. This antibody is useful for detection of GST-tagged proteins on the EpiTitan Histone Peptide array and for detection of GST-tagged fusion proteins by Western Blot, including proteins bound to specific peptides in pull-down experiments.

Antibodies specific for a variety of enzymes that modify histones.

EpiCypher offers a wide range of anti-nucleosome antibodies for various chromatin assays, targeting specific histone post-translational modifications, such as arginine methylation, and unmodified histones. In addition, our SNAP-ChIP™ Certified Antibodies have been extensively validated for ChIP experiments, using recombinant modified designer nucleosomes (dNucs) for in-application testing in a nucleosome context.

Antibodies are a key reagent for chromatin research
Our antibodies can be applied for:

• ChIP-qPCR and ChIP-seq analysis
• Capture or detection in ELISA and immunoblot
• Immunostaining to determine localization of PTMs / nucleosomes

• Reduced cell input : Compatible with as few as 5,000 cells
• Diverse target profiling : histone PTMs and chromatin-interacting proteins (including remodelers)
• Low background: Fewer required sequencing reads per sample (3-5 million)
• Lower cost: Less required antibody and sequencing depth saves money

CUTANA™ CUT&Tag reagents, kits, and assay services enable rapid, cost-effective epigenomic mapping and deliver high quality data with improved resolution compared to ChIP-seq.

Try CUTANA CUT&Tag assays for next-generation epigenomic profiling

• Streamlined 2-day protocol: from samples to NGS libraries in a single tube
• Low background: Fewer required sequencing reads per sample (3-5 million)
• Improved sensitivity: high quality data with ultra-low sample input
• Lower cost: Less required antibody and sequencing depth saves money

Nuclear extracts can be a rich source of chromatin-associated proteins, including transcription factors, chromatin modifying enzymes, and regulatory complexes, and are commonly used as controls or to profile binding interactions of novel proteins. EpiCypher offers high-quality HeLa cell nuclear extracts for these diverse chromatin applications.

Three ways to apply HeLa Nuclear Extracts

• Screening inhibitors
• Substrates for enzymatic assays (e.g. HDACs)
• Controls in immunoblotting

EpiCypher offers mammalian vectors encoding wild-type histone H3.3, as well as the oncogenic H3.3K9M and H3.3K27M. These histones can be used to screen binders or inhibitors that are sensitive to common histone mutations.

ATP-dependent chromatin remodeling enzymes are among the most commonly mutated proteins in cancer, making them a top target for the development of novel therapeutic strategies. The EpiDyne™ platform leverages EpiCypher dNucs as substrates to study nucleosome remodeling. To complement these assays, EpiCypher offers a range of remodeling enzymes, including SMARCA2 and SMARCA4 (the ATPases of the SWI/SNF complex) and the ACF ISWI-type complex.

EpiDyne : advanced tools for next-generation epigenetics assays
EpiDyne Chromatin Remodeling Enzymes can be used in a variety of assay readouts, including:

• FRET – HTS compatible
• Radiometric DAM methyltransferase assays – HTS compatible
• Restriction enzyme accessibility

Recombinant histone proteins are the building block of nucleosomes, and an essential reagent in chromatin research. EpiCypher offers highly pure recombinant histone proteins, including canonical histones (H2A, H2B, H3 and H4) and histone variants, such as H2AZ.1, H2AZ.2 and H2AX.

Application of EpiCypher Recombinant Histones

• Nucleosome binding assays (Chang et al. 2019)
• Have been used to assemble CENP-A variant nucleosomes for microscopy (Stumme-Diers et al. 2019)
• Applied as controls in western blotting (Masuda et al. 2015)

• Bromodomain Proteins : Histone Acetylation
• YEATs Proteins : Histone Acylation
• Chromodomain Proteins : Histone Methylation
• PHD domain proteins : Histone Methylation
• MBT domain proteins : Histone Methylation

Chromatin modifying enzymes target specific histone residues for post-translational modification, including methylation, acetylation, phosphorylation. As such, these proteins drive major changes in chromatin structure, and are important targets in academic and pharmaceutical research. EpiCypher offers a library of histone methyltransferase enzymes and catalytic domains, each highly pure and validated for on-target substrates.

Applications for chromatin modifying enzymes

• Inhibitor assays
• Methyltransferase assays

EpiCypher histone peptide arrays enable rapid, high-throughput screening of chromatin effector proteins, antibodies, and enzymes against a diverse repertoire of single and combinatorial histone PTMs. We offer two versions of histone peptide arrays : the EpiTitan™ array (95 unique modifications) and the EpiTriton™ array (296 unique modifications). Extensive validation and purification of peptides is performed prior to histone peptide array printing to ensure the highest quality product for your studies.

Advantages of EpiCypher Histone Peptide Arrays

• Significantly reduced false-negatives and false positives, reducing time and cost of experiments
• Can Perform multiple experiments on a single array; each sub-array contains 4 replicates of each peptide
• HPLC quality control of all peptides allows full confidence in peptide consistency from array analysis to follow-on experiments
• Are not limited to 20 residues, as all peptides are synthesized and validated prior to array spotting
• Up to 6 unique modifications per peptide allows for greater understanding and utility of peptide protein interaction analysis

EpiCypher modified histone peptides are highly pure and extensively validated, ensuring the highest quality product for chromatin research. Our modified H2A peptides are biotinylated, allowing use in peptide pull down and / or binding assays.

Advantages of EpiCypher Histone Peptides

• 99% pure by MALDI-TOF mass spectrometry and HPLC analysis
• Available as unmodified and modified varieties, including combinatorial PTMs

• 99% pure by MALDI-TOF mass spectrometry and HPLC analysis
• Available as unmodified and modified varieties, including combinatorial PTMs

EpiCypher modified histone peptides are highly pure and extensively validated, ensuring the highest quality product for chromatin research. Our modified H3 peptides are biotinylated, allowing use in peptide pull down and / or binding assays.

Advantages of EpiCypher Histone Peptides

• 99% pure by MALDI-TOF mass spectrometry and HPLC analysis
• Available as unmodified and modified varieties, including combinatorial PTMs

EpiCypher modified histone peptides are highly pure and extensively validated, ensuring the highest quality product for chromatin research. Our modified H4 peptides are biotinylated, allowing use in peptide pull down and / or binding assays.

Advantages of EpiCypher Histone Peptides

• 99% pure by MALDI-TOF mass spectrometry and HPLC analysis
• Available as unmodified and modified varieties, including combinatorial PTMs