Signalchem COVID-19

Research Solutions for Coronavirus – Signalchem

The COVID-19 outbreak due to the novel SARS-CoV-2 virus has turned into a global pandemic infecting hundred thousands of people worldwide. With a rapid rate of infection and a high mortality rate, it is estimated that the novel COVID-19 can infect millions of people all over the globe. Quick intervention at this stage to curb further spread becomes crucial and can only be achieved through expediting critical life science research. Scientists and clinicians around the world are focusing on understanding the molecular biology and mechanisms adapted by COVID-19, and its lethal mode of action. To understand the virus completely and develop safe therapeutic interventions, scientists require a range of trusted molecular biology reagents such as high-quality recombinant proteins related to SARS-CoV-2.
SignalChem, a global leader in cell signaling and protein engineering, has been devoting resources in creating and developing new research tools related to SARS-CoV-2 virus since the outbreak. With the help of advanced bioengineering technology to produce active proteins and AI drug development capability, we have already established expression systems for production of SARS-CoV-2 Spike proteins, RBD fragments, N protein, and other related protein products on a large scale to help detect IgG / IgM in potential patients. These proteins can be used as diagnostic standard antigens and can serve as targets of neutralizing antibodies under development and can also be used by scientists in their development of antibodies to detect SARS-CoV-2 virus in patients. In addition, SignalChem uses mammalian and insect cell expression systems in its production of these protein products as this is more immunogenic than the protein expressed from the E. coli system.
Code Name Size Sample type
N02101-b COVID-19 antigen test 1000 tests Oral/Nasal swabs
N02092-B COVID-19 IgG / IgM antibody detection kit (finger prick) 1000 tests Human blood, serum or plasma
8100805 Sample Collection Kit for Throat Sampling 100 preps Throat swabs
8100812 Sample Collection Kit for Nasal Sampling 100 preps Nasal swabs
Code Name
C19NP-60H Anti 2019-nCoV N-Protein hIgG Antibody
C19NP-60DH Anti 2019-nCoV N-Protein hIgG Antibody, HRP conjugated
C19S1-60H Anti 2019-nCoV Spike Protein hIgG Antibody
C19S1-61H Anti 2019-nCoV Spike Protein hIgG Antibody
C19S1-61MH Anti 2019-nCoV Spike Protein mIgG Antibody
C19S1-A60H Anti 2019-nCoV Spike Protein hIgA Antibody
C19S1-60DH Anti 2019-nCoV Spike Protein hIgG Antibody, HRP conjugated
C19S1-M60H Anti 2019-nCoV Spike Protein hIgM Antibody

SARS-CoV-2 RBD-N Protein Chimera 

Simultaneously binds to anti-S and anti-N protein antibody with high affinity 

RBD-N protein chimera was added into the wells coated with anti 2019-nCoV N-protein hIgG antibody (C19NP-60H).  Binding was detected by incubating the wells with HRP-conjugated anti 2019-nCoV Spike Protein hIgG antibody (C19S1-60DH).

Comparison of SARS-CoV-2 Proteins for Antibody Detection

Detection with Anti-SARS-CoV-2 S Protein Antibody 

Detection with Anti-SARS-CoV-2 N Protein Antibody 

ELISA plates were coated with recombinant SARS-CoV-2 proteins. HRP-conjugated specific antibodies were added to detect the binding. RBD-N chimera protein demonstrated specific binding to anti-S and anti-N antibody with higher affinity comparing to individual S or N protein.

Antibody Pair for High-Sensitivity Detection of SARS-CoV-2

The sensitivity of ELISA using new antibody pair is significantly increased comparing to previous assay.

The ELISA using new antibody pair has limit of detection (LOD) at picomole level.

Code Name
C19SD-876 2019-nCoV S1 Protein ELISA Kit
C19S1-877 2019-nCoV S1 Human IgG ELISA Kit
C19S1-M877 2019-nCoV S1 Human IgM ELISA Kit
C19S1-P877 2019-nCoV S1 Human IgG-IgA-IgM ELISA Kit
C19S1-A877 2019-nCoV S1 Human IgA ELISA Kit
C19NS1-P877 2019-nCoV N-S1 Human IgG-IgA-IgM ELISA Kit
C19NS1-A877 2019-nCoV N-S1 Human IgA ELISA Kit
C19NS1-M877 2019-nCoV N-S1 Human IgM ELISA Kit
C19NS1-877 2019-nCoV N-S1 Human IgG ELISA Kit
C19S1-876 2019-nCoV High Sensitivity S1 Protein ELISA KIT
C19SD-N887 2019-nCoV Neutralization ELISA KIT

COVID-19 ELISA KIT

SignalChem’s SARS-CoV-2 ELISA KITS are effective research and detection tools to study the novel 2019-nCoV and its
molecular interactions with the human host cells. Designed following the principles of ELISA (Enzyme Linked Immunosorbent Assay), our kits can be used for the detection and quantification of SARS-CoV-2 viral protein, human anti-SARS-CoV-2 immunoglobulins, and for research & development of diagnostics and therapeutics against the SARS-CoV-2 virus.

High-Quality ELISA KITS suitable for

  • High Sensitivity Detection and Quantification of SARS-CoV-2 Spike (S1) Protein
  • High Sensitivity Detection and Quantification of Nucleocapsid (N) Protein
  • Detection and Quantification of human anti-SARS-COV-2 immunoglobulins (IgG, IgA, IgM)
  • Detection of binding antibodies that block the interaction of SARS-CoV-2 and human ACE2
  • Research and Development of diagnostics and therapeutics targeting the SARS-CoV-2 virus
Name Catalog Number CAS Registration Number Molecular Formula Purity Chemical Name
Bafilomycin A1 V115-905 88899-55-2 C35H58O9 >98% (HPLC); NMR (conforms) (-)-Bafilomycin A1
Camostat Mesilate S565-906 59721-29-8 C20H22N4O5 • CH3SO3H >98% (HPLC); NMR (Conforms) 4-(2-(2-(dimethylamino)-2-oxoethoxy)-2-oxoethyl)phenyl 4-guanidinobenzoate, methanesul-fonic acid salt; FOY-305; Foipan
Captopril A51-906 62571-86-2 C9H15NO3S >98% (HPLC, TLC); NMR (conforms) (2S)-1-(3-mercapto-2-methyl-propionyl)-L-proline
Chloroquine phosphate C119-905 50-63-5 C18H26ClN3 · 2H3PO4 >98% (TLC); NMR (conforms) N4-(7-Chloro-4-quinolinyl)-N1,N1-dimethyl-1,4-pentanediamine, diphosphate
Hydroxychloroquine T860-905 747-36-4 C18H26ClN3O · H2SO4 >98% (TLC); NMR (conforms) 7-Chloro-4-[4-(N-ethyl-N-b-hydroxyethylamino)-1-methylbutylamino]quinoline, sulfate.
Losartan potassium S35-908 124750-99-8 C22H22ClN6O · K >99% (TLC); NMR (conforms) 2-Butyl-4-chloro-1-{[2′-(1H-tetrazol-5-yl)(1,1′-biphenyl)-4-yl]methyl}-1H-imidazole-5-methanol, potassium salt (DUP-753)
Mycophenolic acid I23-905 24280-93-1 C17H20O6 >98% (HPLC); NMR (conforms) 6-(4-Hydroxy-6-methoxy-7-methyl-3-oxo-5-phthalanyl)-4-methyl-4-hexanoic acid; NSC129185
Niclosamide S54-900 50-65-7 C13H8Cl2N2O4 >98% (TLC); NMR (conforms) 5-Chloro-N-(2-chloro-4-nitrophenyl)-2-hydroxybenzamide
Nitazoxanide P312-905 55981-09-4 C12H9N3O5S >98% (HPLC); NMR (conforms) O-[N-(5-Nitrothiazol-2-yl)carbamoyl]phenyl acetate; NTZ
RVX-208 B17-905B 1044870-39-4 C20H22N2O5 >98% (TLC); NMR (conforms) 2-[4-(2-hydroxyethoxy)-3,5-dimethylphenyl]-5,7-dimethoxy-4(3H)-quinazolinone
Valinomycin V113-908 2001-95-8 C54H90N6O18 >98% (HPLC); NMR (conforms) Cyclo(D-α-hydroxyisovaleryl-D-valyl-L-lactoyl-L-valyl-D-α-hydroxyisovaleryl-D-valyl-L-lactoyl-L-valyl-D-α-hydroxyisovaleryl-D-valyl-L-lactoyl-L-valyl)
Verapamil HCl V114-908 152-11-4 C27H38N2O4 · HCl >98% (TLC); NMR (conforms) α-[3-[[2-(3,4-Dimethoxyphenyl)ethyl]methylamino]propyl]-3,4-dimethoxy-α-(1-methylethyl)benzeneacetonitrile, hydrochloride
Code Name
C19N7-E541H 2019-nCoV NSP7 Protein
C19CL-G241H 2019-nCoV 3CLpro, Active
C19NS-E301H 2019-nCoV NSP10/NSP16 Methyltransferase, Active
C19NS-E311H 2019-nCoV NSP14 Methyltransferase, Active
C19N8-E541H 2019-nCoV NSP8 Protein
C19ND-G241H 2019-nCoV Nucleocapsid protein CTD
C19PL-G241H 2019-nCoV PLpro, Active
C19RP-G241H 2019-nCoV RdRp, Active
A51C2-G342F ACE2 (18-615) Protein
A53C2-G341F ACE2 (18-739) Protein
A52C2-G341F ACE2 (18-740) Protein
A51C2-G341F ACE2 (19-740) Protein
C12CR-E241G Cas12 (LbCpf1), Active
C12CR-E241H Cas12 (LbCpf1), Active
CS13A-E321H Cas13a (LwC2c2), Active
C81D7-G651F CD147 (22-205) Protein
C81D7-G651H CD147 (22-205) Protein
DP4CD-G441H DPP4 (29-766) Protein
M336-380G METTL16, Active
M323-380G METTL3/METTL14, Active
P535-30N Proteinase K, Active (MBG)

Table is showing both binding (Bind) activities to SARS-CoV-2 spike protein RBD and neutralizing (NT) abilities (inhibition of SARS-CoV-2 spike protein RBD binding to ACE-2 receptor) of each neutralizing nanobody measured in functional ELISA.

Code Name
C19S1-644HL Anti 2019-nCoV Spike Protein Neutralizing Nanobody (NBX20014)
C19S1-643HL Anti 2019-nCoV Spike Protein Neutralizing Nanobody (NBX20025)
C19S1-642HL Anti 2019-nCoV Spike Protein Neutralizing Nanobody (NBX20038)
C19S1-654L Anti-2019-nCoV Spike Protein Neutralizing Nanobody (NBX20014)
C19S1-653L Anti-2019-nCoV Spike Protein Neutralizing Nanobody (NBX20025)
C19S1-652L Anti-2019-nCoV Spike Protein Neutralizing Nanobody (NBX20038)
C19S1-641HL Anti 2019-nCoV Spike Protein Neutralizing Nanobody (NBX20010)
Code Name Lineage WHO Nomenclature
C19S1-G241F 2019-nCoV Spike protein S1, Fc Tag Wild Type
C19S1-G241H 2019-nCoV Spike protein S1, HIS Tag Wild Type
C19S2-G241F 2019-nCoV Spike protein S2, Fc Tag Wild Type
C19SD-G241D 2019-nCoV Spike protein RBD, HRP conjugated Wild Type
C19SD-G241F 2019-nCoV Spike protein RBD, Fc Tag Wild Type
C19SD-G241H 2019-nCoV Spike protein RBD, HIS Tag Wild Type
C19NP-G242H 2019-nCoV Nucleocapsid (N) Protein Wild Type
C19NSD-G242H 2019-nCoV Spike RBD-N Protein Chimera Wild Type
C19S1-G232H 2019-nCoV Spike protein S1 (P681H) B.1.1.7 Alpha
C19SD-G231H 2019-nCoV Spike protein RBD (N501Y) B.1.1.7 Alpha
C19SD-G231D 2019-nCoV Spike protein RBD (N501Y), HRP conjugated B.1.1.7 Alpha
C19S1-G239H 2019-nCoV Spike protein S1 (Δ69-70 Δ144 N501Y A570D D614G P681H) B.1.1.7 Alpha
C19S1-G238H 2019-nCoV Spike protein S1 (K417N, E484K, N501Y, D614G) B.1.351 Beta
C19SD-G237H 2019-nCoV spike protein RBD (E484K) B.1.351 Beta
C19SD-G236H 2019-nCoV Spike protein RBD (K417N) B.1.351 Beta
C19SD-G232H 2019-nCoV Spike protein RBD (K417N, E484K, N501Y) (Beta variant) B.1.351 Beta
C19SD-G232D 2019-nCoV Spike protein RBD (K417N, E484K, N501Y), HRP conjugated B.1.351 Beta
C19SD-G235H 2019-nCoV Spike protein RBD (L452R) B.1.427/B.1.429 Epsilon
C19SD-G231DH 2019-nCoV spike protein RBD (L452R, E484Q) B.1.617.1 Kappa
C19SD-G231DD 2019-nCoV Spike protein RBD (L452R, E484Q), HRP conjugated B.1.617.1 Kappa
C19SD-G231CH 2019-nCoV Spike protein RBD (E484Q) B.1.617.1 Kappa
C19SD-G231FH 2019-nCoV Spike Protein RBD (L452R, T478K) B.1.617.2 Delta
C19S1-G231FH 2019-nCoV Spike protein S1 (T19R, ∆157-158, L452R, T478K, D614G, P681R) B.1.617.2 Delta
C19SD-G231FD 2019-nCoV Spike protein RBD (L452R, T478K), HRP conjugated B.1.617.2 Delta
C19S1-G231DH 2019-nCoV spike protein S1 (∆Y145, ∆H146, E484K, D614G) B.1.618
C19SD-G231AD 2019-nCoV Spike protein RBD (K417T, E484K, N501Y), HRP conjugated P.1 Gamma
C19SD-G239H 2019-nCoV Spike protein RBD (K417T) P.1 Gamma
C19SD-G231AH 2019-nCoV Spike protein RBD (K417T, E484K and N501Y) P.1 Gamma
C19SD-G231LH 2019-nCoV Spike protein RBD (K417N, L452R, T478K)
C19SD-G233H 2019-nCoV Spike protein RBD (N439K)
C19SD-G231EH 2019-nCoV Spike protein RBD (N440K)
C19SD-G231BH 2019-nCoV Spike protein RBD (S477G)
C19SD-G238H 2019-nCoV Spike protein RBD (S477N)
C19SD-G234H 2019-nCoV Spike protein RBD (Y453F)
C19S1-G231BH 2019-nCoV Spike protein S1 (Δ69-70, N439K, D614G)
C19S1-G231AH 2019-nCoV Spike protein S1 (Δ69-70, Y453F, D614G)
C19S1-G231H 2019-nCoV Spike protein S1 (D614G)

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