Signalchem COVID-19

Research Solutions for Coronavirus – Signalchem

The COVID-19 outbreak due to the novel SARS-CoV-2 virus has turned into a global pandemic infecting hundred thousands of people worldwide. With a rapid rate of infection and a high mortality rate, it is estimated that the novel COVID-19 can infect millions of people all over the globe. Quick intervention at this stage to curb further spread becomes crucial and can only be achieved through expediting critical life science research. Scientists and clinicians around the world are focusing on understanding the molecular biology and mechanisms adapted by COVID-19, and its lethal mode of action. To understand the virus completely and develop safe therapeutic interventions, scientists require a range of trusted molecular biology reagents such as high-quality recombinant proteins related to SARS-CoV-2.
SignalChem, a global leader in cell signaling and protein engineering, has been devoting resources in creating and developing new research tools related to SARS-CoV-2 virus since the outbreak. With the help of advanced bioengineering technology to produce active proteins and AI drug development capability, we have already established expression systems for production of SARS-CoV-2 Spike proteins, RBD fragments, N protein, and other related protein products on a large scale to help detect IgG / IgM in potential patients. These proteins can be used as diagnostic standard antigens and can serve as targets of neutralizing antibodies under development and can also be used by scientists in their development of antibodies to detect SARS-CoV-2 virus in patients. In addition, SignalChem uses mammalian and insect cell expression systems in its production of these protein products as this is more immunogenic than the protein expressed from the E. coli system.
Code Name Size Sample type
N02101-b COVID-19 antigen test 1000 tests Oral/Nasal swabs
N02092-B COVID-19 IgG / IgM antibody detection kit (finger prick) 1000 tests Human blood, serum or plasma
8100805 Sample Collection Kit for Throat Sampling 100 preps Throat swabs
8100812 Sample Collection Kit for Nasal Sampling 100 preps Nasal swabs
Code Name Type
A51C2-G341F Recombinant human angiotensin-converting enzyme 2 (ACE2) extracellular domain (19-740) Protein
A51C2-G342F Recombinant human angiotensin-converting enzyme 2 (ACE2) extracellular domain (18-615) Protein
C19SD-G241F Recombinant 2019-nCoV Spike protein S1 subunit, receptor-binding domain (RBD) (319-541) Protein
C19SD-G241H Recombinant SARS-CoV-2 Coronavirus (COVID-19) Spike protein S1 subunit, receptor-binding domain (RBD) (319-541)  C-terminal his tag Protein
C19NSD-G242H 2019-nCoV Spike RBD-N Protein Chimera Protein
C19S1-G241H Recombinant 2019-nCoV Spike protein S1 (16-685) Protein
C19RP-G241H 2019-nCoV RdRp, Active Protein
C19PL-G241H Recombinant 2019-nCoV PLpro/papain-like protease, part of a large replicase polyprotein 1ab (E1564-Y1882) – his tag Protein
C19CL-G241H 2019-nCoV 3CLpro, Active Protein
C12CR-G241G Cas12(LbCpf1) Protein Protein
M323-380G Full-length recombinant human METTL3/METTL14 complex Protein
C19NP-60H Anti-2019-nCoV N-Protein Recombinant Human Monoclonal Antibody Antibody
C19S1-60H Anti-2019-nCoV Spike (SC0175) Recombinant Human Monoclonal Antibody Antibody
C19S1-61H Anti-2019nCoV Spike Protein Antibody
C19S1-61MH Anti-2019-nCoV Spike (mFc) Recombinant mAb with human Fab and mouse Fc region Antibody
H38-60M-100 Anti-Human IgG, Unconjugated Antibody
H38M-60M-100 Anti-Human IgM, Unconjugated Antibody

SARS-CoV-2 RBD-N Protein Chimera 

Simultaneously binds to anti-S and anti-N protein antibody with high affinity 

RBD-N protein chimera was added into the wells coated with anti 2019-nCoV N-protein hIgG antibody (C19NP-60H).  Binding was detected by incubating the wells with HRP-conjugated anti 2019-nCoV Spike Protein hIgG antibody (C19S1-60DH).

Comparison of SARS-CoV-2 Proteins for Antibody Detection

Detection with Anti-SARS-CoV-2 S Protein Antibody 

Detection with Anti-SARS-CoV-2 N Protein Antibody 

ELISA plates were coated with recombinant SARS-CoV-2 proteins. HRP-conjugated specific antibodies were added to detect the binding. RBD-N chimera protein demonstrated specific binding to anti-S and anti-N antibody with higher affinity comparing to individual S or N protein.

Antibody Pair for High-Sensitivity Detection of SARS-CoV-2

The sensitivity of ELISA using new antibody pair is significantly increased comparing to previous assay.

The ELISA using new antibody pair has limit of detection (LOD) at picomole level.

Code Name Type
C19SD-876 2019-nCoV S1 Protein ELISA Kit 96 well plate pre-coated with recombinant human ACE2 protein
C19S1-877 2019-nCoV S1 Human IgG ELISA Kit 96 well plate pre-coated with recombinant 2019-nCoV spike protein
C19S1-M877 2019-nCoV S1 Human IgM ELISA Kit ELISA kit
C19S1-P877 2019-nCoV S1 Human IgG-IgA-IgM ELISA Kit ELISA kit
C19NS1-P877 2019-nCoV N-S1 Human IgG-IgA-IgM ELISA Kit ELISA kit
C19NS1-A877 2019-nCoV N-S1 Human IgA ELISA Kit ELISA kit
C19NS1-M877 2019-nCoV N-S1 Human IgM ELISA Kit ELISA kit
C19NS1-877 2019-nCoV N-S1 Human IgG ELISA Kit ELISA kit
Name Catalog Number CAS Registration Number Molecular Formula Purity Chemical Name
Bafilomycin A1 V115-905 88899-55-2 C35H58O9 >98% (HPLC); NMR (conforms) (-)-Bafilomycin A1
Camostat Mesilate S565-906 59721-29-8 C20H22N4O5 • CH3SO3H >98% (HPLC); NMR (Conforms) 4-(2-(2-(dimethylamino)-2-oxoethoxy)-2-oxoethyl)phenyl 4-guanidinobenzoate, methanesul-fonic acid salt; FOY-305; Foipan
Captopril A51-906 62571-86-2 C9H15NO3S >98% (HPLC, TLC); NMR (conforms) (2S)-1-(3-mercapto-2-methyl-propionyl)-L-proline
Chloroquine phosphate C119-905 50-63-5 C18H26ClN3 · 2H3PO4 >98% (TLC); NMR (conforms) N4-(7-Chloro-4-quinolinyl)-N1,N1-dimethyl-1,4-pentanediamine, diphosphate
Hydroxychloroquine T860-905 747-36-4 C18H26ClN3O · H2SO4 >98% (TLC); NMR (conforms) 7-Chloro-4-[4-(N-ethyl-N-b-hydroxyethylamino)-1-methylbutylamino]quinoline, sulfate.
Losartan potassium S35-908 124750-99-8 C22H22ClN6O · K >99% (TLC); NMR (conforms) 2-Butyl-4-chloro-1-{[2′-(1H-tetrazol-5-yl)(1,1′-biphenyl)-4-yl]methyl}-1H-imidazole-5-methanol, potassium salt (DUP-753)
Mycophenolic acid I23-905 24280-93-1 C17H20O6 >98% (HPLC); NMR (conforms) 6-(4-Hydroxy-6-methoxy-7-methyl-3-oxo-5-phthalanyl)-4-methyl-4-hexanoic acid; NSC129185
Niclosamide S54-900 50-65-7 C13H8Cl2N2O4 >98% (TLC); NMR (conforms) 5-Chloro-N-(2-chloro-4-nitrophenyl)-2-hydroxybenzamide
Nitazoxanide P312-905 55981-09-4 C12H9N3O5S >98% (HPLC); NMR (conforms) O-[N-(5-Nitrothiazol-2-yl)carbamoyl]phenyl acetate; NTZ
RVX-208 B17-905B 1044870-39-4 C20H22N2O5 >98% (TLC); NMR (conforms) 2-[4-(2-hydroxyethoxy)-3,5-dimethylphenyl]-5,7-dimethoxy-4(3H)-quinazolinone
Valinomycin V113-908 2001-95-8 C54H90N6O18 >98% (HPLC); NMR (conforms) Cyclo(D-α-hydroxyisovaleryl-D-valyl-L-lactoyl-L-valyl-D-α-hydroxyisovaleryl-D-valyl-L-lactoyl-L-valyl-D-α-hydroxyisovaleryl-D-valyl-L-lactoyl-L-valyl)
Verapamil HCl V114-908 152-11-4 C27H38N2O4 · HCl >98% (TLC); NMR (conforms) α-[3-[[2-(3,4-Dimethoxyphenyl)ethyl]methylamino]propyl]-3,4-dimethoxy-α-(1-methylethyl)benzeneacetonitrile, hydrochloride

SignalChem Biotech responds to the COVID19 Pandemic

SignalChem Biotech Inc. COVID-19 strategies

SUPPORT

outstanding technical support

PRODUCT

we offer a full product guarantee

DELIVERY

we offer free delivery to UK universities and non profit organisations