| Name | Cat. No. | Size |
| Direct qPCR ProbesMaster | PCR-396S | 2 x 1,25 ml (250 reactions x 20 μl) |
| Direct qPCR ProbesMaster | PCR-396L | 10 x 1,25 ml (1250 reactions x 20 μl) |
| Direct qPCR ProbesMaster highROX | PCR-397S | 2 x 1,25 ml (250 reactions x 20 μl) |
| Direct qPCR ProbesMaster highROX | PCR-397L | 10 x 1,25 ml (1250 reactions x 20 μl) |
Real-Time PCR / qPCR
JBS qPCR Master Series allows quantitative real-time analysis of DNA samples based on detection of labeled DNA probes. Master Mixes contain all reagents required for qPCR (except template, primer and labeled fluorescent probe) in a premixed 2x concentrated solution, including hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of initial denaturation to prevent extension of nonspecifically annealed primers and primer-dimer formation.
Both qPCR ProbesMaster mixes with and without UNG (Uracil-N-Glycosylase) are available in combination with low ROX and high ROX as reference dye for cycler-internal signal normalization. To facilitate pipetting into opaque plasticware a blue dyed version of the qPCR ProbesMaster Mixes is available. Mixes are recommended for use with Dual Labeled Fluorescent Probes, e.g. TaqMan®, Molecular Beacons or FRET probes.
| Name | Cat. No. | Size |
| qPCR ProbesMaster | PCR-360S | 2 x 1,25 ml (250 reactions x 20 μl) |
| qPCR ProbesMaster | PCR-360L | 10 x 1,25 ml (1250 reactions x 20 μl) |
| qPCR ProbesMaster lowROX | PCR-361S | 2 x 1,25 ml (250 reactions x 20 μl) |
| qPCR ProbesMaster lowROX | PCR-361L | 10 x 1,25 ml (1250 reactions x 20 μl) |
| qPCR ProbesMaster highROX | PCR-362S | 2 x 1,25 ml (250 reactions x 20 μl) |
| qPCR ProbesMaster highROX | PCR-362L | 10 x 1,25 ml (1250 reactions x 20 μl) |
| qPCR ProbesMaster UNG | PCR-363S | 2 x 1,25 ml (250 reactions x 20 μl) |
| qPCR ProbesMaster UNG | PCR-363L | 10 x 1,25 ml (1250 reactions x 20 μl) |
| qPCR ProbesMaster UNG lowROX | PCR-364S | 2 x 1,25 ml (250 reactions x 20 μl) |
| qPCR ProbesMaster UNG lowROX | PCR-364L | 10 x 1,25 ml (1250 reactions x 20 μl) |
| qPCR ProbesMaster UNG highROX | PCR-365S | 2 x 1,25 ml (250 reactions x 20 μl) |
| qPCR ProbesMaster UNG highROX | PCR-365L | 10 x 1,25 ml (1250 reactions x 20 μl) |
Lyophilisates – Preloaded and Stable at Room Temperature
Ready-to-Use Lyophilisates are delivered in PCR reaction tube strips or 96-well plates preloaded with a complete master mix in a dry, room temperature stable format. Facilitate handling with ready-to-use lyophilisates: no need for freezing, thawing or pipetting on ice and the few remaining pipetting steps minimize the risk of errors or contaminations.
Development, optimization and scale-up of custom-specific lyophilisates
Freeze-dryed mixes are stable at ambient temperatures and significantly reduce contamination risk, turnaround time and total costs.
Lyophilisation service of Jena Bioscience at a glance
Optimization
- adaptation of in-house lyophilisates to customized applications
- modification of liquid custom-established assays for lyophilisation
- optimization of the lyophilisation process to improve product functionality, stability and appearance
Lyophilisation
- addition of custom-synthesized primers and internal/positive control DNA
- up-scaling from small batch sizes to several thousand assay per batch
- highly efficient cryo- and lyoprotectors with minimal influence on PCR available
Packaging
- lyophilisation of PCR mixes in single PCR tubes, 8-well tube strips, 96-well plates or custom-specific formats
- thermosealing into aluminium composite foil according to individual demands
- custom labeling
Ready-to-Use Lyophilisates containing custom primers are custom made master mix lyophilisates comprising primers, polymerase, dNTPs and reaction buffer. Provide us with primer sequence and we deliver preloaded PCR tubes and plates ready-made for your special application. The only thing you still have to do is adding template DNA, filling up with water and starting the cycler!
The JBS qPCR SybrMaster Series is designed for quantitative real-time analysis of DNA samples. The mixes contain all reagents required for qPCR (except template and primers) in a premixed 2x concentrated ready-to-use solution. The mixes are recommended for routine PCR applications, high throughput PCR or genotyping and provide an improved specificity and sensitivity when amplifying low-copy-number targets or working with complex backgrounds.
The fluorescent DNA stain SYBR® Green intercalates into the amplification product during the PCR process and allows the direct quantification of target DNA without the need of sequence-specific labeled probes (i.g. TaqMan® Probes).
High Resolution Melting (HRM) analysis is a powerful tool for detection of mutations, polymorphisms and epigenetic differences in DNA samples. It is a fast and cost effective alternative to other genotyping technologies.
UNG (Uracil-N-Glycosylase) eliminates carry-over contamination of DNA from previous PCR reactions. An UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation.
SYBR® Green Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable, providing convenience during routine handling. SYBR® Green is in contrast to EvaGreen® not recommended for high-resolution melting curve analysis (HRM).
| Name | Cat. No. | Size |
| qPCR SybrMaster | PCR-372S | 2 x 1,25 ml (2x conc.) |
| qPCR SybrMaster | PCR-372L | 10 x 1,25 ml (2x conc.) |
| qPCR SybrMaster lowROX | PCR-373S | 2 x 1,25 ml (2x conc.) |
| qPCR SybrMaster lowROX | PCR-373L | 10 x 1,25 ml (2x conc.) |
| qPCR SybrMaster highROX | PCR-374S | 2 x 1,25 ml (2x conc.) |
| qPCR SybrMaster highROX | PCR-374L | 10 x 1,25 ml (2x conc.) |
| qPCR SybrMaster UNG | PCR-375S | 2 x 1,25 ml (2x conc.) |
| qPCR SybrMaster UNG | PCR-375L | 10 x 1,25 ml (2x conc.) |
| qPCR SybrMaster UNG lowROX | PCR-376S | 2 x 1,25 ml (2x conc.) |
| qPCR SybrMaster UNG lowROX | PCR-376L | 10 x 1,25 ml (2x conc.) |
| qPCR SybrMaster UNG highROX | PCR-377S | 2 x 1,25 ml (2x conc.) |
| qPCR SybrMaster UNG highROX | PCR-377L | 10 x 1,25 ml (2x conc.) |
EvaGreen® is a registered trademark and licensed for sale by Biotium, Inc., Hayward, CA, USA.
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.
JBS qPCR GreenMaster Series is designed for quantitative real-time analysis of DNA samples including High Resolution Melting (HRM) curve analysis. The mixes contain all reagents required for qPCR (except template and primers) in a premixed 2x concentrated ready-to-use solution. The mixes are recommended for routine PCR applications, high throughput PCR or genotyping and provide an improved specificity and sensitivity when amplifying low-copy-number targets or working with complex backgrounds.
The fluorescent DNA stain EvaGreen® intercalates into the amplification product during the PCR process and allows the direct quantification of target DNA without the need of sequence-specific labeled probes (i.g. TaqMan® Probes).
High Resolution Melting (HRM) analysis is a powerful tool for detection of mutations, polymorphisms and epigenetic differences in DNA samples. It is a fast and cost effective alternative to other genotyping technologies.
UNG (Uracil-N-Glycosylase) eliminates carry-over contamination of DNA from previous PCR reactions. An UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation.
EvaGreen® Fluorescent DNA Stain is a DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR) and high-resolution DNA melting curve analysis (HRM). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.
| Name | Cat. No. | Size |
| qPCR GreenMaster highROX | PCR-368S | 2 x 1,25 ml (2x conc.) |
| qPCR GreenMaster highROX | PCR-368L | 10 x 1,25 ml (2x conc.) |
| qPCR GreenMaster UNG | PCR-369S | 2 x 1,25 ml (2x conc.) |
| qPCR GreenMaster UNG | PCR-369L | 10 x 1,25 ml (2x conc.) |
EvaGreen® is a registered trademark and licensed for sale by Biotium, Inc., Hayward, CA, USA.
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.
The JBS qPCR Multiplex Master Series has been specially developed and optimized for simultaneous real-time analysis of >4 target sequences in a single tube. The system overcomes multiplex limitations of conventional qPCR probe mixes combining with an above-average robustness for a multitude of known PCR inhibitors and an excellent sensitivity for amplification of lowest template amounts.
The 2x concentrated master mixes contain all reagents required for qPCR (except template, primers and labeled fluorescent probes) including a highly processive antibody-inhibited hot-start polymerase and ultra-pure dNTPs.
The mixes provide an extremely stringent automatic hot-start allowing reaction set-up and temporary storage at room temperature prior to PCR.
qPCR Multiplex Master Mixes are available in combination with low ROX and high ROX as reference dye for cycler-internal signal normalization. All mixes are recommended for use with Dual Labeled Fluorescent Probes, e.g. TaqMan®, Molecular Beacons or FRET probes.
| Name | Cat. No. | Size |
| qPCR MultiplexMaster | PCR-321S | 2 x 1,25 ml (2x conc.) |
| qPCR MultiplexMaster | PCR-321L | 10 x 1,25 ml (2x conc.) |
| qPCR MultiplexMaster lowROX | PCR-322S | 2 x 1,25 ml (2x conc.) |
| qPCR MultiplexMaster lowROX | PCR-322L | 10 x 1,25 ml (2x conc.) |
| qPCR MultiplexMaster highROX | PCR-323S | 2 x 1,25 ml (2x conc.) |
| qPCR MultiplexMaster highROX | PCR-323L | 10 x 1,25 ml (2x conc.) |
Helpful tools for Real-Time PCR, to test the function or control the quality of qPCR polymerases and qPCR assay conditons.
| Name | Cat. No. | Size |
| Thermolabile UNG (Uracil N-Glycosylase) | PCR-353 | 200 units |
| SYBR® Green Fluorescent DNA Stain | PCR-378 | 500 μl x 100 μM |
| EvaGreen® Fluorescent DNA Stain | PCR-379 | 500 μl x 100 μM |
| ROX Reference Dye | PCR-351 | 1 ml (25 μM) |
EvaGreen® is a registered trademark and licensed for sale by Biotium, Inc., Hayward, CA, USA.
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.
Dual Labeled Fluorescent Probes are DNA oligonucleotides of 20-30 bp carrying a fluorophore (5′-end) and a quencher (3′-end). The labeled probe hybridizes sequence-specifically to its complementary section of the amplicon. During DNA extension of each PCR cycle, the fluorophore reporter is being cleaved and released. The resulting, detectable fluorescence signal is proportional to the amount of accumulated PCR product. All labeled probes are purified by HPLC and quality checked by MALDI-TOF. Select from Jena Bioscience’s extensive reporter/quencher repertoire or inquire for alternative combinations (info@stratech.co.uk).
BHQ-1 Quencher
Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.
A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.
| Name | Cat. No. | Size |
| 5’ ATTO-425 / 3’ BHQ-1® | FP-414-05 | 5 to 9 nmol |
| 5’ ATTO-425 / 3’ BHQ-1® | FP-414-10 | 10 to 19 nmol |
| 5’ ATTO-425 / 3’ BHQ-1® | FP-414-20 | 20 to 29 nmol |
| 5’ ATTO-425 / 3’ BHQ-1® | FP-414-30 | 30 to 49 nmol |
| 5’ ATTO-425 / 3’ BHQ-1® | FP-414-50 | 50 to 70 nmol |
| 5’ LC®Cyan500 / 3’ BHQ-1® | FP-120-05 | 5 to 9 nmol |
| 5’ LC®Cyan500 / 3’ BHQ-1® | FP-120-10 | 10 to 19 nmol |
| 5’ LC®Cyan500 / 3’ BHQ-1® | FP-120-20 | 20 to 29 nmol |
| 5’ LC®Cyan500 / 3’ BHQ-1® | FP-120-30 | 30 to 49 nmol |
| 5’ LC®Cyan500 / 3’ BHQ-1® | FP-120-50 | 50 to 70 nmol |
| 5’ 6-FAM / 3’ BHQ-1® | FP-180-05 | 5 to 9 nmol |
| 5’ 6-FAM / 3’ BHQ-1® | FP-180-10 | 10 to 19 nmol |
| 5’ 6-FAM / 3’ BHQ-1® | FP-180-20 | 20 to 29 nmol |
| 5’ 6-FAM / 3’ BHQ-1® | FP-180-30 | 30 to 49 nmol |
| 5’ 6-FAM / 3’ BHQ-1® | FP-180-50 | 50 to 70 nmol |
| 5’ Fluo / 3’ BHQ-1® | FP-190-05 | 5 to 9 nmol |
| 5’ Fluo / 3’ BHQ-1® | FP-190-10 | 10 to 19 nmol |
| 5’ Fluo / 3’ BHQ-1® | FP-190-20 | 20 to 29 nmol |
| 5’ Fluo / 3’ BHQ-1® | FP-190-30 | 30 to 49 nmol |
| 5’ Fluo / 3’ BHQ-1® | FP-190-50 | 50 to 70 nmol |
| 5’ FITC / 3’ BHQ-1® | FP-185-05 | 5 to 9 nmol |
| 5’ FITC / 3’ BHQ-1® | FP-185-10 | 10 to 19 nmol |
| 5’ FITC / 3’ BHQ-1® | FP-185-20 | 20 to 29 nmol |
| 5’ FITC / 3’ BHQ-1® | FP-185-30 | 30 to 49 nmol |
| 5’ FITC / 3’ BHQ-1® | FP-185-50 | 50 to 70 nmol |
| 5’ ATTO-495 / 3’ BHQ-1® | FP-418-05 | 5 to 9 nmol |
| 5’ ATTO-495 / 3’ BHQ-1® | FP-418-10 | 10 to 19 nmol |
| 5’ ATTO-495 / 3’ BHQ-1® | FP-418-20 | 20 to 29 nmol |
| 5’ ATTO-495 / 3’ BHQ-1® | FP-418-30 | 30 to 49 nmol |
| 5’ ATTO-495 / 3’ BHQ-1® | FP-418-50 | 50 to 70 nmol |
| 5’ TET / 3’ BHQ-1® | FP-122-05 | 5 to 9 nmol |
| 5’ TET / 3’ BHQ-1® | FP-122-10 | 10 to 19 nmol |
| 5’ TET / 3’ BHQ-1® | FP-122-20 | 20 to 29 nmol |
| 5’ TET / 3’ BHQ-1® | FP-122-30 | 30 to 49 nmol |
| 5’ TET / 3’ BHQ-1® | FP-122-50 | 50 to 70 nmol |
| 5’ ATTO-520 / 3’ BHQ-1® | FP-410-05 | 5 to 9 nmol |
| 5’ ATTO-520 / 3’ BHQ-1® | FP-410-10 | 10 to 19 nmol |
| 5’ ATTO-520 / 3’ BHQ-1® | FP-410-20 | 20 to 29 nmol |
| 5’ ATTO-520 / 3’ BHQ-1® | FP-410-30 | 30 to 49 nmol |
| 5’ ATTO-520 / 3’ BHQ-1® | FP-410-50 | 50 to 70 nmol |
| 5’ JOE / 3’ BHQ-1® | FP-123-05 | 5 to 9 nmol |
| 5’ JOE / 3’ BHQ-1® | FP-123-10 | 10 to 19 nmol |
| 5’ JOE / 3’ BHQ-1® | FP-123-20 | 20 to 29 nmol |
| 5’ JOE / 3’ BHQ-1® | FP-123-30 | 30 to 49 nmol |
| 5’ JOE / 3’ BHQ-1® | FP-123-50 | 50 to 70 nmol |
| 5’ Yakima Yellow / 3’ BHQ-1® | FP-134-05 | 5 to 9 nmol |
| 5’ Yakima Yellow / 3’ BHQ-1® | FP-134-10 | 10 to 19 nmol |
| 5’ Yakima Yellow / 3’ BHQ-1® | FP-134-20 | 20 to 29 nmol |
| 5’ Yakima Yellow / 3’ BHQ-1® | FP-134-30 | 30 to 49 nmol |
| 5’ Yakima Yellow / 3’ BHQ-1® | FP-134-50 | 50 to 70 nmol |
| 5’ HEX / 3’ BHQ-1® | FP-124-05 | 5 to 9 nmol |
| 5’ HEX / 3’ BHQ-1® | FP-124-10 | 10 to 19 nmol |
| 5’ HEX / 3’ BHQ-1® | FP-124-20 | 20 to 29 nmol |
| 5’ HEX / 3’ BHQ-1® | FP-124-30 | 30 to 49 nmol |
| 5’ HEX / 3’ BHQ-1® | FP-124-50 | 50 to 70 nmol |
| 5’ ATTO-Rho6G / 3’ BHQ-1® | FP-404-05 | 5 to 9 nmol |
| 5’ ATTO-Rho6G / 3’ BHQ-1® | FP-404-10 | 10 to 19 nmol |
| 5’ ATTO-Rho6G / 3’ BHQ-1® | FP-404-20 | 20 to 29 nmol |
| 5’ ATTO-Rho6G / 3’ BHQ-1® | FP-404-30 | 30 to 49 nmol |
| 5’ ATTO-Rho6G / 3’ BHQ-1® | FP-404-50 | 50 to 70 nmol |
BHQ-2 Quencher
Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.
A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.
| Products & Ordering | ||
| 5’ 6-FAM / 3’ BHQ-2® FP-181 | 5’ Cy3B / 3’ BHQ-2® FP-423 | 5’ DY-480XL / 3’ BHQ-2® FP-194 |
| Ex: 495 nm / Em: 520 nm | Ex: 559 nm / Em: 570 nm | Ex: 500 nm / Em: 630 nm |
| 5’ Fluo / 3’ BHQ-2® FP-191 | 5’ TAMRA / 3’ BHQ-2® FP-126 | 5’ LC®Red640 / 3’ BHQ-2® FP-135 |
| Ex: 495 nm / Em: 520 nm | Ex: 564 nm / Em: 579 nm | Ex: 625 nm / Em: 640 nm |
| 5’ FITC / 3’ BHQ-2® FP-186 | 5’ ROX / 3’ BHQ-2® FP-127 | 5’ ATTO-Rho14 / 3’ BHQ-2® FP-199 |
| Ex: 490 nm / Em: 525 nm | Ex: 576 nm / Em: 601 nm | Ex: 625 nm / Em: 646 nm |
| 5’ TET / 3’ BHQ-2® FP-164 | 5’ TexasRed / 3’ BHQ-2® FP-132 | 5’ Cy5 / 3’ BHQ-2® FP-136 |
| Ex: 521 nm / Em: 536 nm | Ex: 586 nm / Em: 610 nm | Ex: 646 nm / Em: 662 nm |
| 5’ HEX / 3’ BHQ-2® FP-133 | 5’ LC®Red610 / 3’ BHQ-2® FP-131 | 5’ Cy5.5 / 3’ BHQ-2® FP-137 |
| Ex: 535 nm / Em: 556 nm | Ex: 590 nm / Em: 610 nm | Ex: 683 nm / Em: 705 nm |
| 5’ ATTO-Rho6G / 3’ BHQ-2® FP-405 | 5’ ATTO-Rho13 / 3’ BHQ-2® FP-196 | 5’ IRD700 / 3’ BHQ-2® FP-138 |
| Ex: 533 nm / Em: 557 nm | Ex: 603 nm / Em: 627 nm | Ex: 685 nm / Em: 705 nm |
| 5’ Cy3 / 3’ BHQ-2® FP-125 | ||
| Ex: 546 nm / Em: 563 nm | ||
BHQ-3 Quencher
Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.
A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.
| Products & Ordering | ||
| 5’ ATTO-Rho13 / 3’ BHQ-3® FP-197 | 5’ ATTO-Rho14 / 3’ BHQ-3® FP-400 | 5’ Cy5.5 / 3’ BHQ-3® FP-142 |
| Ex: 603 nm / Em: 627 nm | Ex: 625 nm / Em: 646 nm | Ex: 683 nm / Em: 705 nm |
| 5’ LC®Red640 / 3’ BHQ-3® FP-144 | 5’ Cy5 / 3’ BHQ-3® FP-141 | 5’ IRD700 / 3’ BHQ-3® FP-143 |
| Ex: 625 nm / Em: 640 nm | Ex: 646 nm / Em: 662 nm | Ex: 685 nm / Em: 705 nm |
BBQ®-650 Quencher
Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.
A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.
| Products & Ordering | ||
| 5’ HEX / 3’ BBQ®-650 FP-169 | 5’ TexasRed / 3’ BBQ®-650 FP-173 | 5’ ATTO-Rho14 / 3’ BBQ®-650 FP-401 |
| Ex: 535 nm / Em: 556 nm | Ex: 586 nm / Em: 610 nm | Ex: 625 nm / Em: 646 nm |
| 5’ ATTO-Rho6G / 3’ BBQ®-650 FP-407 | 5’ LC®Red610 / 3’ BBQ®-650 FP-174 | 5’ Cy5 / 3’ BBQ®-650 FP-176 |
| Ex: 533 nm / Em: 557 nm | Ex: 590 nm / Em: 610 nm | Ex: 646 nm / Em: 662 nm |
| 5’ Cy3 / 3’ BBQ®-650 FP-170 | 5’ ATTO-Rho13 / 3’ BBQ®-650 FP-198 | 5’ Cy5.5 / 3’ BBQ®-650 FP-177 |
| Ex: 546 nm / Em: 563 nm | Ex: 603 nm / Em: 627 nm | Ex: 683 nm / Em: 705 nm |
| 5’ TAMRA / 3’ BBQ®-650 FP-171 | 5’ DY-480XL / 3’ BBQ®-650 FP-195 | 5’ IRD700 / 3’ BBQ®-650 FP-178 |
| Ex: 564 nm / Em: 579 nm | Ex: 500 nm / Em: 630 nm | Ex: 685 nm / Em: 705 nm |
| 5’ ROX / 3’ BBQ®-650 FP-172 | 5’ LC®Red640 / 3’ BBQ®-650 FP-175 | |
| Ex: 576 nm / Em: 601 nm | Ex: 625 nm / Em: 640 nm | |
ECLIPSE Quencher
Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.
A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.
| Products & Ordering | ||
| 5’ ATTO-390 / 3’ ECLIPSE FP-422 | 5’ ATTO-495 / 3’ ECLIPSE FP-419 | 5’ ATTO-Rho6G / 3’ ECLIPSE FP-406 |
| Ex: 390 nm / Em: 476 nm | Ex: 498 nm / Em: 526 nm | Ex: 533 nm / Em: 557 nm |
| 5’ ATTO-425 / 3’ ECLIPSE FP-415 | 5’ TET / 3’ ECLIPSE FP-153 | 5’ Cy3 / 3’ ECLIPSE FP-162 |
| Ex: 439 nm / Em: 485 nm | Ex: 521 nm / Em: 536 nm | Ex: 546 nm / Em: 563 nm |
| 5’ LC®Cyan500 / 3’ ECLIPSE FP-151 | 5’ ATTO-520 / 3’ ECLIPSE FP-411 | 5’ TAMRA / 3’ ECLIPSE FP-157 |
| Ex: 450 nm / Em: 500 nm | Ex: 517 nm / Em: 538 nm | Ex: 564 nm / Em: 579 nm |
| 5’ 6-FAM / 3’ ECLIPSE FP-182 | 5’ JOE / 3’ ECLIPSE FP-154 | 5’ ROX / 3’ ECLIPSE FP-158 |
| Ex: 495 nm / Em: 520 nm | Ex: 522 nm / Em: 548 nm | Ex: 576 nm / Em: 601 nm |
| 5’ Fluo / 3’ ECLIPSE FP-192 | 5’ Yakima Yellow / 3’ ECLIPSE FP-155 | 5’ TEXAS RED / 3’ ECLIPSE FP-159 |
| Ex: 495 nm / Em: 520 nm | Ex: 530 nm / Em: 549 nm | Ex: 586 nm / Em: 610 nm |
| 5’ FITC / 3’ ECLIPSE FP-187 | 5’ HEX / 3’ ECLIPSE FP-156 | 5’ LC®Red610 / 3’ ECLIPSE FP-160 |
| Ex: 490 nm / Em: 525 nm | Ex: 535 nm / Em: 556 nm | Ex: 590 nm / Em: 610 nm |
DABCYL Quencher
Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.
A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.
| Products & Ordering | ||
| 5’ ATTO-390 / 3’ DABCYL FP-421 | 5’ FITC / 3’ DABCYL FP-188 | 5’ Yakima Yellow / 3’ DABCYL FP-117 |
| Ex: 390 nm / Em: 476 nm | Ex: 490 nm / Em: 525 nm | Ex: 530 nm / Em: 549 nm |
| 5’ ATTO-425 / 3’ DABCYL FP-413 | 5’ ATTO-495 / 3’ DABCYL FP-417 | 5’ HEX / 3’ DABCYL FP-115 |
| Ex: 439 nm / Em: 485 nm | Ex: 498 nm / Em: 526 nm | Ex: 535 nm / Em: 556 nm |
| 5’ LC®Cyan500 / 3’ DABCYL FP-111 | 5’ TET / 3’ DABCYL FP-113 | 5’ ATTO-Rho6G / 3’ DABCYL FP-403 |
| Ex: 450 nm / Em: 500 nm | Ex: 521 nm / Em: 536 nm | Ex: 533 nm / Em: 557 nm |
| 5’ 6-FAM / 3’ DABCYL FP-183 | 5’ ATTO-520 / 3’ DABCYL FP-409 | 5’ TAMRA / 3’ DABCYL FP-161 |
| Ex: 495 nm / Em: 520 nm | Ex: 517 nm / Em: 538 nm | Ex: 564 nm / Em: 579 nm |
| 5’ Fluo / 3’ DABCYL FP-193 | 5’ JOE / 3’ DABCYL FP-114 | |
| Ex: 495 nm / Em: 520 nm | Ex: 522 nm / Em: 548 nm | |
TAMRA Quencher
Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.
A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.
| Products & Ordering | ||
| 5’ ATTO-390 / 3’ TAMRA FP-420 | 5’ ATTO-495 / 3’ TAMRA FP-416 | 5’ HEX / 3’ TAMRA FP-105 |
| Ex: 390 nm / Em: 476 nm | Ex: 498 nm / Em: 526 nm | Ex: 535 nm / Em: 556 nm |
| 5’ ATTO-425 / 3’ TAMRA FP-412 | 5’ TET / 3’ TAMRA FP-103 | 5’ ATTO-Rho6G / 3’ TAMRA FP-402 |
| Ex: 439 nm / Em: 485 nm | Ex: 521 nm / Em: 536 nm | Ex: 533 nm / Em: 557 nm |
| 5’ 6-FAM / 3’ TAMRA FP-179 | 5’ ATTO-520 / 3’ TAMRA FP-408 | 5’ ROX / 3’ TAMRA FP-168 |
| Ex: 495 nm / Em: 520 nm | Ex: 517 nm / Em: 538 nm | Ex: 576 nm / Em: 601 nm |
| 5’ Fluo / 3’ TAMRA FP-189 | 5’ JOE / 3’ TAMRA FP-104 | 5’ Cy5.5 / 3’ TAMRA FP-167 |
| Ex: 495 nm / Em: 520 nm | Ex: 522 nm / Em: 548 nm | Ex: 683 nm / Em: 705 nm |
| 5’ FITC / 3’ TAMRA FP-184 | 5’ Yakima Yellow / 3’ TAMRA FP-106 | 5’ IRD700 / 3’ TAMRA FP-166 |
| Ex: 490 nm / Em: 525 nm | Ex: 530 nm / Em: 549 nm | Ex: 685 nm / Em: 705 nm |
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