Why HRP as an enzyme reporter system
The widespread adoption of HRP as enzyme reporter system is primarily due to three factors. First, HRP has the ability to amplify weak signals and enhance detectability of low abundance target molecules. Second, the relatively small size of HRP (~44 kDa) compared to other reporter enzymes (e.g. alkaline phosphatase (~140 kDa)) further improves intracellular penetration into samples, reduces steric hindrance and minimizes immunoreactivity loss. Third, is the high turnover rate and good stability of HRP that enables rapid and strong signal generation.
Visualizing HRP
By itself, the HRP enzyme, or conjugates thereof, have very little value. Its presence can only be visualized with the addition of a suitable substrate that, when oxidized by HRP using hydrogen peroxide, produces a quantifiable signal. A variety of HRP substrates have been developed and commercialized to exploit the desirable features of this enzyme. These substrates fall into three distinct categories: chromogenic, fluorogenic or chemiluminescent substrates. The choice of substrate depends on the type of instrument to be used for signal-detection, and should be optimized for the intended system and detection limit.
HRP Labeling Tools & Services
ReadiUse™ Preactivated HRP reagents and Buccutite™ Peroxidase (HRP) Antibody Conjugation kits offer a convenient and robust process for conjugating antibodies to HRP. Featuring streamlined protocols, produce stable HRP-antibody conjugates in two simple mixing steps.
For custom conjugation of HRP to an antibody in our catalog or as a stand-alone service for customer-supplied materials, refer to our Bioconjugation Services.
HRP Conjugates
ReadiUse™ Preactivated HRP reagents and Buccutite™ Peroxidase (HRP) Antibody Conjugation kits offer a convenient and robust process for conjugating antibodies to HRP. Featuring streamlined protocols, produce stable HRP-antibody conjugates in two simple mixing steps.
For custom conjugation of HRP to an antibody in our catalog or as a stand-alone service for customer-supplied materials, refer to our Bioconjugation Services.
HRP Substrates
Choose form our large assortment of chromogenic, fluorogenic and luminescent HRP substrates available in a variety of sensitivities – compatible with any instrument setup. For detection of low-abundance targets in cells and tissues, use iFluor™ Styramide reagents compatible with ICC, IHC and FISH procedures. For assistance selecting a substrate, see tables below.
Amplite™ Assay Kits
Amplite™ enzyme-based assays provide a sensitive and simple method for the quantitation of a diverse array of analytes, including glucose, catalase, xanthine, acetylcholine and choline, as well as, hydrogen peroxide.
