› › AAT Bioquest

AAT Bioquest are well known for their photometric detection fluorescence and luminescent technologies.

Their products have applications in drug discovery and diagnostic research and they are continuously expanding their product portfolio.

They are well known for their i Fluor kits and Fluo-8 the trite still calcium indicator.

AAT strive to innovate and push the boundaries of assay technology.

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Products

Enzymes
Chemical Reagents
Bioconjugation
Physiological Probes
Antibodies and Proteomics
Cell Structures and Organelles
Cellular Processes
Gelite™ Safe DNA Gel Stain

Top Products

  • Amplite™ enzyme-based detection platform, optimised for measuring horseradish peroxidase (HRP), alkaline phosphatase, luciferase, beta-galactosidase, lactamase, protein kinases, protein phosphatases, phosphodiesterases, proteases, cytochrome P450, histone deacetylase (HDAC) and cell signalling molecules such as NAD/NADH, NADP/NADPH, IP3, cAMP and cGMP etc.
  • Cell Explorer™ cell labelling kits, a complete set of tools for tracking live cells.
  • Cell Navigator™ cell staining kits, a complete set of tools for selectively labelling subcellular structures of live, fixed and dead cells.
  • Cell Meter™ cellular functional assay kits, a complete set of tools for functional analysis of cellular events.
  • FP Fluor™ superior fluorescent labelling dyes, optimised for developing fluorescence polarisation-based assays.
  • iFluor™ superior fluorescent labelling dyes, optimised for labelling proteins and nucleic acids.
  • NIR Fluor™ superior fluorescent labelling dyes, optimised for in vivo imaging.
  • PhosphoWorks™ detection platform; a set of tools for detection of ATP, ADP, AMP, phosphate, pyrophosphate, phosphoproteins and phosphopeptides.
  • Prolite™ protein detection platform; a sensitive and robust tool for detection of proteins in solutions and solid surfaces (such as gels and membranes).
  • Quest View™ colourimetric protease platform; a sensitive and robust tool for detection of diagnostic protease and glycosidase biomarkers in rapid tests.
  • ReadiView Biotin™ labelling platform, a robust tool for biotinylation.
  • RatioWorks™ superior cellular dyes; a sensitive and robust tool for ratio imaging of live cells.
  • Screen Quest™ assay kits, a set of HTS-ready tools for high throughput screening of biochemical and cellular targets such as protein kinases, proteases, HDAC, cell apoptosis and cytoxicity, GPCR, ion channels, ADME/metabolism and transporters.
  • Tide Fluor™ superior fluorescent labelling dyes, optimised for labelling nucleotides and peptides.
  • Tide Quencher™ non-fluorescent labelling dyes, optimised for labelling FRET nucleotides and peptide.
  • TR Fluor™superior fluorescent labelling dyes, optimised for developing time-resolved fluorescence-based assays.

Catalogues

Labeling Antibodies and Biopolymers
Fluorescent Infrared Dyes and Probes
Secondary Detection Probes and Kits
FRET Building Blocks
Nucleic Acid Detection
Reactive Oxygen Species (ROS) Detection
Physiological Probes and Assay Kits
Oligo Labeling Reagents
Cell Metabolism and Signalling Assays
Fluorescence Cell Imaging Probes
Peptide Labeling Reagents
Cell Viability and Proliferation
Fluorescence Microscopy
Phycobiliproteins and Tandem Conjugates
Calcium Detection Probes
Enzyme Probes and Assay Kits
Cell Apoptosis and Proliferation

Recent Application Notes

Webinars

Calcium and GPCR
Fluorescence Antibody Labeling
Fundamentals of Flow Cytometry

Featured Web-Based Tools

What are the 4 steps of PCR?

PCR consists of the four following steps:

  1. Denaturation by Heat: double-stranded DNA is separated into two single strands, by a process called denaturation which occurs at temperatures higher than 90 degrees Celsius. Heat breaks hydrogen bonds between the base pairs, while the stronger bonds between deoxyribose and phosphates, remain intact.
  2. Annealing Primer to Target Sequence: before the target sequence (generally between 100-600 base pairs) is replicated, it must be targeted using primers that target the ends of the target DNA sequence by annealing (binding) to the complementary sequence. Annealing occurs at lower temperature (between 40 and 65 degrees Celsius) which depends on the length and base sequence of the primers.
  3. Extension: after annealing, the temperature is increased to 72 degrees Celsius and the Taq DNA polymerase enzyme is used for replicating the DNA strands. During synthesis (or extension), two identical double stranded DNA molecules are synthesized.
  4. End of the First PCR Cycle: after which the process is repeated generally between 25 and 35 times.

Interactive Spectrum Viewer

Interactive Spectrum Viewer

Allows users to view and compare spectral (excitation and emission) data. This tool is essential for experimental planning and choosing the right fluorophore/label.

Extinction Coefficient Finder

Extinction Coefficient Finder

Search a large database of fluorescent compounds. Extinction coefficient is an important factor in quantifying probe “brightness” and is useful for calculations of concentration and degree of labelling.

Concentration & Molecular Weight

DNA molecular weight calculator – When performing a PCR or gel run, the molecular weight of a sense strand of RNA can be important to know. This calculator converts a string of nucleotides into a molecular weight value. Additionally, the antisense strand of the DNA is provided, along with its own molecular weight.
Peptide and protein molecular weight calculator – Amino acids, the constituent organic compounds making up peptides and proteins, form long chains that determine shape and function. This calculator provides the molecular weight of a peptide or protein based on a string of amino acids given.
Protein concentration calculator – For many assays, it is critical to know the concentration of a protein solution prior to experimentation. The concentration of a protein solution can be determined using its absorbance value at its maximum absorbance wavelength and a derived form of the Beer-Lambert law.
RNA molecular weight calculator – When performing a PCR or gel run, the molecular weight of a sense strand of RNA can be important to know. This calculator converts a string of nucleotides into a molecular weight value. Additionally, the antisense strand of the DNA is provided, along with its own molecular weight.

Data Analysis & Regression

4PL calculator – Fits a symmetrical sigmoidal, “s”, shaped curve for experimental data. Generates equation and graph.
EC50 calculator – In pharmacology, the EC50 represents the concentration of a substance which results in half of its maximal response. This calculator will determine the EC50 given experimental data.
IC50 calculator – The IC50 of an antagonist is the concentration at which half of the inhibitory effect is achieved. It is an important value in pharmacology as it characterizes a drug’s potency on biological processes. This calculator determines the IC50 given experimental data.
Linear, Logarithmic, Semi-Log Regression Calculator – This calculator determines a best fit linear function using the least-squares method. It will provide four different linear regressions: linear-linear, log-linear, linear-log and log-log.

Experiment Planning

Buffer preparations – A list of common physiological buffers used in research. Includes buffers such as acetate buffer, HBSS (Hanks’ balanced salt solution) and PBS. Recipe is automatically calculated based on desired volume. pH and molarity can be calculated automatically for acid-base derived buffers.
Serial dilution calculator – Serial dilutions are a common step in biological research, crafting a specially-tuned solution for use in later steps of an experiment. The serial dilution calculator generates a series of instructions to guide you through the dilution of any solution, given an initial concentration and dilution factor, or a concentration range.

Spectral Data

Extinction coefficient finder – The extinction coefficient represents the degree to which a compound attenuates light at a specific wavelength. Often, the specific wavelength of interest corresponds to the compound’s maximal absorbance. Together with quantum yield, the extinction coefficient is an important parameter in a compound’s “brightness”.
Spectrum viewer – An important step in experimental planning is choosing the right fluorophore. The spectrum viewer web application allows user to compare a vast library of fluorophores based on excitation and emission values. Explore which fluorophores are compatible with a particular instrument, laser, or filter set. Plan multiplex assays by choosing spectra with minimal overlap. Or choose spectra with optimal overlap for developing tandem and FRET based labels.

Other Tools

Degree of labeling calculator – One of the most important metrics in bioconjugation is the degree of labeling (DOL). This value represents the number of labels attached to each target (ie. protein). An optimal DOL is critical to the success of a conjugation. DOL values that are too high or too low tend to yield poor performing conjugates. This tool helps calculate the DOL of common label-target pairs. Requires absorbance values from a spectrophotometer or similar instrument.

Power Styramide™ Signal Amplification (PSA™)

iFluor™ 488 Styramide Power Styramide™ Signal Amplification (PSA™) is a novel enzymatic amplification method used to detect low-abundance targets in Read More...

Actin

Probes for Imaging Actin Filaments Actin is a highly conserved family of proteins that form microfilaments. Due to its intracellular Read More...

Golgi Apparatus

The Golgi apparatus is a complex of vesicles and folded membranes within the cytoplasm of most eukaryotic cells. It plays Read More...

Endoplasmic Reticulum (ER)

The endoplasmic reticulum (ER) is an organelle present in most eukaryotic cells. It is primarily responsible for the synthesis and Read More...

Plasma Membrane

The plasma membrane is a thin semi-permeable membrane that separates the interior of all cells from their extracellular environment. It Read More...

Lysosomes

Lysosomes are the “waste-disposal” system of the cell digesting unwanted materials and cellular debris in the cytoplasm. They are membrane-enclosed Read More...

Nucleus

The cell nucleus is a membrane-bound organelle that contains a majority of the cell's genetic material (DNA) in the form Read More...

Mitochondria

Probes for Mitochondria Mitochondria are a distinctive, membrane-bound organelle present within all eukaryotic cells. As the primary site of ATP Read More...

Cell Structures and Organelles

Organelles are specialized structures that play a critical part in cellular function. Nearly all cellular activities are facilitated by organelles Read More...

Reactive Oxygen Species (ROS)

Reactive oxygen species (ROS) are chemically reactive, oxygen-containing molecules that are naturally produced as a byproduct of cellular metabolism. Under Read More...

Intracellular Ions

The steady-state maintenance of disproportionate concentrations of inorganic cations and anions is a feature of living cells. Homeostatic regulation of Read More...

Intracellular pH

Intracellular pH plays an important modulating role in various cellular events, including cell growth, calcium regulation, enzymatic activity, receptor-mediated signal Read More...

G-Protein-Coupled Receptors (GPCR)

G-Protein Coupled Receptors (GPCRs) are known as seven transmembrane spanning receptors or metabotropic receptors. They are the largest family of Read More...

DNA and RNA

DNA encodes all genetic information and is the blueprint from which all biological life is created. It acts as a Read More...

Physiological Probes

Physiological probes investigate cellular pathways and activities by precisely measuring variations in targeted ions and biomolecules. Several physiological processes are Read More...

Rhodamines

Rhodamines are xanthene derivatives structurally related to fluorescein, but with additional chemical substitutions that shift their excitation and emission spectra to Read More...

Cluster of Differentiation (CD)

Cluster of differentiation (CD) are cell surface molecules expressed on the cells of the immune system, which play key roles Read More...

Chemical Reagents

The dyes that make quantitative experiments possible must be specific, stable, and not interfere with the natural behavior of the Read More...

Fluoresceins

Fluorescein derivatives are one of the most commonly used fluorescent labels for biological detection in flow cytometry and immunofluorescnece due Read More...

Peptide and Oligonucleotide Labeling

Dye-labeled peptides and oligonucleotides are important tools in biochemical and cellular studies. Fluorescent peptides and oligonucleotides have been extensively used Read More...