AAT Bioquest are well known for their photometric detection fluorescence and luminescent technologies.

Their products have applications in drug discovery and diagnostic research and they are continuously expanding their product portfolio.

They are well known for their i Fluor kits and Fluo-8 the trite still calcium indicator.

AAT strive to innovate and push the boundaries of assay technology.

Top Products

  • Amplite™ enzyme-based detection platform, optimised for measuring horseradish peroxidase (HRP), alkaline phosphatase, luciferase, beta-galactosidase, lactamase, protein kinases, protein phosphatases, phosphodiesterases, proteases, cytochrome P450, histone deacetylase (HDAC) and cell signalling molecules such as NAD/NADH, NADP/NADPH, IP3, cAMP and cGMP etc.
  • Cell Explorer™ cell labelling kits, a complete set of tools for tracking live cells.
  • Cell Navigator™ cell staining kits, a complete set of tools for selectively labelling subcellular structures of live, fixed and dead cells.
  • Cell Meter™ cellular functional assay kits, a complete set of tools for functional analysis of cellular events.
  • FP Fluor™ superior fluorescent labelling dyes, optimised for developing fluorescence polarisation-based assays.
  • iFluor™ superior fluorescent labelling dyes, optimised for labelling proteins and nucleic acids.
  • NIR Fluor™ superior fluorescent labelling dyes, optimised for in vivo imaging.
  • PhosphoWorks™ detection platform; a set of tools for detection of ATP, ADP, AMP, phosphate, pyrophosphate, phosphoproteins and phosphopeptides.
  • Prolite™ protein detection platform; a sensitive and robust tool for detection of proteins in solutions and solid surfaces (such as gels and membranes).
  • Quest View™ colourimetric protease platform; a sensitive and robust tool for detection of diagnostic protease and glycosidase biomarkers in rapid tests.
  • ReadiView Biotin™ labelling platform, a robust tool for biotinylation.
  • RatioWorks™ superior cellular dyes; a sensitive and robust tool for ratio imaging of live cells.
  • Screen Quest™ assay kits, a set of HTS-ready tools for high throughput screening of biochemical and cellular targets such as protein kinases, proteases, HDAC, cell apoptosis and cytoxicity, GPCR, ion channels, ADME/metabolism and transporters.
  • Tide Fluor™ superior fluorescent labelling dyes, optimised for labelling nucleotides and peptides.
  • Tide Quencher™ non-fluorescent labelling dyes, optimised for labelling FRET nucleotides and peptide.
  • TR Fluor™superior fluorescent labelling dyes, optimised for developing time-resolved fluorescence-based assays.


Recent Application Notes


Time: Jun 24, 2020 16:00 (BST)
Topic: A Series of New & Improved Calcium Indicators for Functional Analysis of GPCRs and Calcium Channel Targets
Description: G-Protein Coupled Receptors (GPCR) are the largest family of membrane receptors in the entire human genome. As the name suggests, they function by coupling with various G-proteins to regulate intracellular signalling pathways, notably including the cAMP pathway and Ca+2 pathway. This free webinar will focus on the function and practical applications of newly developed and improved calcium indicators for the investigation of these incredibly important proteins. Pre-submitted questions will be answered immediately following the presentation.
Topics include:
GPCRS and Calcium Signaling
Calcium Indicator Development
AAT Bioquest Calcium detection probes
Calcium assays (GPCRs_Gq and calcium channels)
cAMP (TR-FRET) and Calcium assays (GPCRs_Gs and Gi/o)

Featured Web-Based Tools

PCR consists of the four following steps:

  1. Denaturation by Heat: double-stranded DNA is separated into two single strands, by a process called denaturation which occurs at temperatures higher than 90 degrees Celsius. Heat breaks hydrogen bonds between the base pairs, while the stronger bonds between deoxyribose and phosphates, remain intact.
  2. Annealing Primer to Target Sequence: before the target sequence (generally between 100-600 base pairs) is replicated, it must be targeted using primers that target the ends of the target DNA sequence by annealing (binding) to the complementary sequence. Annealing occurs at lower temperature (between 40 and 65 degrees Celsius) which depends on the length and base sequence of the primers.
  3. Extension: after annealing, the temperature is increased to 72 degrees Celsius and the Taq DNA polymerase enzyme is used for replicating the DNA strands. During synthesis (or extension), two identical double stranded DNA molecules are synthesized.
  4. End of the First PCR Cycle: after which the process is repeated generally between 25 and 35 times.

Interactive Spectrum Viewer

Allows users to view and compare spectral (excitation and emission) data. This tool is essential for experimental planning and choosing the right fluorophore/label.

Extinction Coefficient Finder

Search a large database of fluorescent compounds. Extinction coefficient is an important factor in quantifying probe “brightness” and is useful for calculations of concentration and degree of labelling.

Concentration & Molecular Weight

DNA molecular weight calculator – When performing a PCR or gel run, the molecular weight of a sense strand of RNA can be important to know. This calculator converts a string of nucleotides into a molecular weight value. Additionally, the antisense strand of the DNA is provided, along with its own molecular weight.
Peptide and protein molecular weight calculator – Amino acids, the constituent organic compounds making up peptides and proteins, form long chains that determine shape and function. This calculator provides the molecular weight of a peptide or protein based on a string of amino acids given.
Protein concentration calculator – For many assays, it is critical to know the concentration of a protein solution prior to experimentation. The concentration of a protein solution can be determined using its absorbance value at its maximum absorbance wavelength and a derived form of the Beer-Lambert law.
RNA molecular weight calculator – When performing a PCR or gel run, the molecular weight of a sense strand of RNA can be important to know. This calculator converts a string of nucleotides into a molecular weight value. Additionally, the antisense strand of the DNA is provided, along with its own molecular weight.

Data Analysis & Regression

4PL calculator – Fits a symmetrical sigmoidal, “s”, shaped curve for experimental data. Generates equation and graph.
EC50 calculator – In pharmacology, the EC50 represents the concentration of a substance which results in half of its maximal response. This calculator will determine the EC50 given experimental data.
IC50 calculator – The IC50 of an antagonist is the concentration at which half of the inhibitory effect is achieved. It is an important value in pharmacology as it characterizes a drug’s potency on biological processes. This calculator determines the IC50 given experimental data.
Linear, Logarithmic, Semi-Log Regression Calculator – This calculator determines a best fit linear function using the least-squares method. It will provide four different linear regressions: linear-linear, log-linear, linear-log and log-log.

Experiment Planning

Buffer preparations – A list of common physiological buffers used in research. Includes buffers such as acetate buffer, HBSS (Hanks’ balanced salt solution) and PBS. Recipe is automatically calculated based on desired volume. pH and molarity can be calculated automatically for acid-base derived buffers.
Serial dilution calculator – Serial dilutions are a common step in biological research, crafting a specially-tuned solution for use in later steps of an experiment. The serial dilution calculator generates a series of instructions to guide you through the dilution of any solution, given an initial concentration and dilution factor, or a concentration range.

Spectral Data

Extinction coefficient finder – The extinction coefficient represents the degree to which a compound attenuates light at a specific wavelength. Often, the specific wavelength of interest corresponds to the compound’s maximal absorbance. Together with quantum yield, the extinction coefficient is an important parameter in a compound’s “brightness”.
Spectrum viewer – An important step in experimental planning is choosing the right fluorophore. The spectrum viewer web application allows user to compare a vast library of fluorophores based on excitation and emission values. Explore which fluorophores are compatible with a particular instrument, laser, or filter set. Plan multiplex assays by choosing spectra with minimal overlap. Or choose spectra with optimal overlap for developing tandem and FRET based labels.

Other Tools

Degree of labeling calculator – One of the most important metrics in bioconjugation is the degree of labeling (DOL). This value represents the number of labels attached to each target (ie. protein). An optimal DOL is critical to the success of a conjugation. DOL values that are too high or too low tend to yield poor performing conjugates. This tool helps calculate the DOL of common label-target pairs. Requires absorbance values from a spectrophotometer or similar instrument.

Calcium Indicators for GPCR Analysis & Calcium Targets

AAT Bioquest Webinar

A Series of New & Improved Calcium Indicators for Functional Analysis of GPCRs and Calcium Channel Targets G-Protein Coupled Receptors Read More...

Amplite™ Dyes and Kits

The Amplite™ product series is composed of enzyme-based substrates and assays optimized for measuring horseradish peroxidase (HRP), luciferase, phosphodiesterases, proteases, Read More...


The use and study of enzymes is a key part of biochemistry and cellular biology. Enzymes act as a regulatory Read More...


 Transferases are a class of enzymes that catalyze the transfer of specific functional groups (e.g. a methyl or glycosyl group) Read More...


A protease is an enzyme that conducts proteolysis, i.e., the protein catabolism by hydrolysis of the peptide bonds that link Read More...


 A polymerase is an enzyme (EC whose central biological function is the synthesis of polymers or nucleic acids. DNA Read More...


Firefly Luciferase The most versatile and common reporter gene is the luciferase of the North American firefly photinus pyralis. The Read More...


Kinases are a family of enzymes that catalyze the transfer of a phosphate moiety from a high-energy, phosphate-donating molecule, such Read More...


Dehydrogenases are family of enzymes belonging to the group oxidoreductase. These enzymes catalyze the oxidation of a substrate by transferring Read More...

Alkaline Phosphatase (AP)

Phosphatases are a class of enzymes that play a key role in the dephosphorylation of organic compounds. Using water as Read More...


Research Solutions for Coronavirus – AAT Bioquest

Contact usPlace an Order Covidyte™ Peptide Substrate Series Developed For Researchers In The Fight Against COVID-19 The inhibition of viral Read More...

Real-Time qRT-PCR and qPCR reagents for COVID-19 research

Request a QuoteTechnical SupportPlace an Order Below is a comprehensive list of all our Real-Time qRT-PCR and qPCR reagents for Read More...

50% OFF Transfectamine™ 5000

Half Price Transfection Reagent

Coupon code T50 must be provided at the time of sale for promotion to apply. Offer only applies to purchases of transfection Read More...


AAT Bioquest Annexin V conjugates and assay kits offer Promo code: ANXV25 Valid until December 31st 2019 See below the Read More...

Palm-sized device with unlimited possibilities

Introducing CytoCite™ Fluorometer with Cloud-Integration

The CytoCite™ Fluorometer is the first cloud-integrated, handheld fluorometer. This device, when paired with the appropriate bioassays, can be used Read More...

Calbryte™ series

Calbryte™ series The Calbryte™ series is a family of fluorescent dyes developed to monitor intracellular calcium. It includes three novel Read More…

First for Antibodies

Stratech Scientific have been successful in a recent antibodies tender from the Southern Universities Purchasing Consortium. In the award criterion Read More…

Full Spectrum Multiple Labelling

Calcium acts as a universal second messenger in a variety of cells. Numerous functions of all types of cells are Read More…

Calcium Dyes Past, Present and Future

A History of Calcium Indicators in Life Science

Introduction to calcium dyes Calcium ions (Ca2+) play a crucial physiological role in the body: signalling, blood clotting, in cellular Read More…

Better Intracellular Retention

Cal-520™, Green Fluorescent Calcium Dyes

Cal-520™ provides the most robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilisation. Cal-520™ AM is a new fluorogenic Read More…