AAT Bioquest are well known for their photometric detection fluorescence and luminescent technologies.

Their products have applications in drug discovery and diagnostic research and they are continuously expanding their product portfolio.

They are well known for their i Fluor kits and Fluo-8 the trite still calcium indicator.

AAT strive to innovate and push the boundaries of assay technology.


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Featured Web-Based Tools

Interactive Spectrum Viewer

Extinction Coefficient Finder

 

Allows users to view and compare spectral (excitation and emission) data. This tool is essential for experimental planning and choosing the right fluorophore/label.

 

Search a large database of fluorescent compounds. Extinction coefficient is an important factor in quantifying probe “brightness” and is useful for calculations of concentration and degree of labelling.

Concentration & Molecular Weight

DNA molecular weight calculator – When performing a PCR or gel run, the molecular weight of a sense strand of RNA can be important to know. This calculator converts a string of nucleotides into a molecular weight value. Additionally, the antisense strand of the DNA is provided, along with its own molecular weight.
Peptide and protein molecular weight calculator – Amino acids, the constituent organic compounds making up peptides and proteins, form long chains that determine shape and function. This calculator provides the molecular weight of a peptide or protein based on a string of amino acids given.
Protein concentration calculator – For many assays, it is critical to know the concentration of a protein solution prior to experimentation. The concentration of a protein solution can be determined using its absorbance value at its maximum absorbance wavelength and a derived form of the Beer-Lambert law.
RNA molecular weight calculator – When performing a PCR or gel run, the molecular weight of a sense strand of RNA can be important to know. This calculator converts a string of nucleotides into a molecular weight value. Additionally, the antisense strand of the DNA is provided, along with its own molecular weight.

Data Analysis & Regression

4PL calculator – Fits a symmetrical sigmoidal, “s”, shaped curve for experimental data. Generates equation and graph.
EC50 calculator – In pharmacology, the EC50 represents the concentration of a substance which results in half of its maximal response. This calculator will determine the EC50 given experimental data.

IC50 calculator – The IC50 of an antagonist is the concentration at which half of the inhibitory effect is achieved. It is an important value in pharmacology as it characterizes a drug’s potency on biological processes. This calculator determines the IC50 given experimental data.

Linear, Logarithmic, Semi-Log Regression Calculator – This calculator determines a best fit linear function using the least-squares method. It will provide four different linear regressions: linear-linear, log-linear, linear-log and log-log.

Experiment Planning

Buffer preparations – A list of common physiological buffers used in research. Includes buffers such as acetate buffer, HBSS (Hanks’ balanced salt solution) and PBS. Recipe is automatically calculated based on desired volume. pH and molarity can be calculated automatically for acid-base derived buffers.
Serial dilution calculator Serial dilutions are a common step in biological research, crafting a specially-tuned solution for use in later steps of an experiment. The serial dilution calculator generates a series of instructions to guide you through the dilution of any solution, given an initial concentration and dilution factor, or a concentration range.

Spectral Data

Extinction coefficient finder – The extinction coefficient represents the degree to which a compound attenuates light at a specific wavelength. Often, the specific wavelength of interest corresponds to the compound’s maximal absorbance. Together with quantum yield, the extinction coefficient is an important parameter in a compound’s “brightness”.
Spectrum viewer – An important step in experimental planning is choosing the right fluorophore. The spectrum viewer web application allows user to compare a vast library of fluorophores based on excitation and emission values. Explore which fluorophores are compatible with a particular instrument, laser, or filter set. Plan multiplex assays by choosing spectra with minimal overlap. Or choose spectra with optimal overlap for developing tandem and FRET based labels.

Other Tools

Degree of labeling calculator – One of the most important metrics in bioconjugation is the degree of labeling (DOL). This value represents the number of labels attached to each target (ie. protein). An optimal DOL is critical to the success of a conjugation. DOL values that are too high or too low tend to yield poor performing conjugates. This tool helps calculate the DOL of common label-target pairs. Requires absorbance values from a spectrophotometer or similar instrument.

 


25% OFF ALL ANNEXIN V PRODUCTS

AAT Bioquest Annexin V conjugates and assay kits offer Promo code: ANXV25 Valid until December 31st 2019 See below the Read More...

Palm-sized device with unlimited possibilities

Introducing CytoCite™ Fluorometer with Cloud-Integration

The CytoCite™ Fluorometer is the first cloud-integrated, handheld fluorometer. This device, when paired with the appropriate bioassays, can be used Read More...

Calbryte™ series

Calbryte™ series The Calbryte™ series is a family of fluorescent dyes developed to monitor intracellular calcium. It includes three novel Read More…

First for Antibodies

Stratech Scientific have been successful in a recent antibodies tender from the Southern Universities Purchasing Consortium. In the award criterion Read More…

Full Spectrum Multiple Labelling

Calcium acts as a universal second messenger in a variety of cells. Numerous functions of all types of cells are Read More…

Calcium Dyes Past, Present and Future

A History of Calcium Indicators in Life Science

Introduction to calcium dyes Calcium ions (Ca2+) play a crucial physiological role in the body: signalling, blood clotting, in cellular Read More…

Better Intracellular Retention

Cal-520™, Green Fluorescent Calcium Dyes

Cal-520™ provides the most robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilisation. Cal-520™ AM is a new fluorogenic Read More…

Calcium Dyes

Cal-520™ & Cal-520FF™ Cal-520™ provides a robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilisation. Cal-520™ AM is a Read More…

iFluor™ Kits

AAT Bioquest’s iFluor™ dyes are Optimised for labelling proteins, in particular, antibodies.   These dyes are bright, photostable and have minimal Read More…

Resisting Cell Death

In order to survive and proliferate, cancer cells must overcome and avoid the diverse range and number of cell death Read More…

Visualisation

  Overview There are many ways of visualising your results; some of them more visual than others! Stratech supply sensitive Read More…

The Rapid & Sensitive Detection of NAD, NADH, NADP & NADPH Using Amplite™ Assay Kits

NAD, NADH, NADP and NADPH are important cofactors in cells. AAT Bioquest Amplite™ NAD(P)/NAD(P)H assay kits have very low background Read More…

JC–10 a Superior JC-1 Replacement

Although JC-1 is widely used in many labs, its poor water solubility makes it hard to use for some applications. Read More…

Cal-520™ for detection of intracellular calcium mobilization

Cal-520™ AM is our newest fluorogenic calcium-sensitive dye with a significantly improved signal to noise ratio and intracellular retention compared Read More…