Buccutite™ Crosslinking Technology
Buccutite™ crosslinking technology offers a truly unique and trouble-free method of labeling antibodies with another macromolecule, such as an enzyme or phycobiliprotein. Compared to conventional techniques for protein-protein conjugation, such as the labor intensive SMCC chemical labeling method, Buccutite™ conjugation is significantly more robust and less demanding. These kits allow optimal labeling of microgram or milligram amounts of antibodies in less than two hours, with no purification necessary and 100% recovery of antibody. With Buccutite™ labeling kits, routinely prepare covalently-labeled conjugates that can tolerate the rigorous handling and washing that is required in a typical immunoassay (e.g. ELISA, IHC and Western blot) or flow cytometry application. AAT Bioquest offers Buccutite™ antibody labeling kits for preparing horseradish peroxidase (HRP), alkaline phosphatase (AP), PE, APC, PerCP and tandem dye antibody conjugates.
Too busy to perform your own conjugation or having difficulty finding the right labeling kit, not to worry AAT Bioquest’s bioconjugation service can prepare the custom antibody conjugate you need.