Abcepta is a leading manufacturer of primary antibodies with more than fifteen years of experience.
We supply quality antibodies and related products drug discovery and academic laboratories worldwide.
Our focus is on targets covering the major research areas impacting health and disease, including cancer, metabolism, cardiovascular, neuroscience, and stem cells.
In addition, Abcepta offers the highest quality services including custom antibody development, custom peptide synthesis and custom protein expression. Synthesis of difficult peptides, including long peptides, is a core competency of Abcepta. High quality, rapid turnaround and personal support for all Abcepta services are guaranteed.
Discover what Abcepta can do for you. We are here to be your partner for scientific research and discovery.
Products labeled with a “Crown” meet stringent requirements in terms of specificity, sensitivity, and reproducibility.
Abcepta is committed to preserving our natural resources and the environment. As part of this effort, we provide you with digital versions of all our brochures and posters below. However, if you prefer ordering a printed version, please fill out the form with your information and we will be happy to mail you one.
Abcepta is a leading manufacturer of custom antibodies with over 20,000 antibodies developed over the last decade. Our team applies decades to offer tailored, flexible solutions producing antibodies matched to your needs. Provide your own immunogen or work with our world-class synthetic peptide chemistry group to design an epitope-specific and immunogenic peptide to meet your specifications for yield, purity, and target specificity. Our experience applies across a broad range of antibody applications, from workhorse Western Blot reagents, up to robust polyclonal anti-drug antibodies (ADA) used as positive controls for serum analysis as part of biotherapeutic safety assays.
Additionally, Abcepta can develop a pre-optimized antibody validated in the assay of your choosing. Please contact us to learn more about these and other service offerings.
|Peptide antigen design and preparation:|
|Peptide antigen synthesis up to 18 AA residues with purity > 95%||14|
|Peptide antigen conjugation to KLH carrier protein||7|
|OR Recombinant protein antigen（supplied by customer, >95% pure by SDS Page)||7|
|Two subcutaneous injections of 200 ug conjugated peptide/protein with adjuvant per rabbit Three subcutaneous injections of 100 ug conjugated peptide/protein with adjuvant per rabbit Collect test bleeds. Conduct ELISA analysis on test bleed #1, and test bleed #2 if #1 is negative Five production bleeds per rabbit Terminal bleed by exsanguination (±40 ml serum/rabbit), final ELISA analysis||69|
|Peptide affinity purification for typical total yield of 2-10 mg IgG from 2 rabbits for delivery to customer||7|
|Optional: Application Validation Test WB, IHC, IF, FC||3|
Abcepta is a leading manufacturer of off the shelf and custom antibodies with over 20,000 antibodies developed over the last decade. Our ELITE Monoclonal Antibody Custom Service* is ideal for projects requiring high specificity and a low background. Monoclonal antibodies are highly homogenous and allow for unlimited production with sustained specificity.
We offer primary Monoclonal Antibody Service in Balb/c mice, as well as antibody characterization, validation optimization, and scale up. Our highly experienced team can develop a pre-optimized antibody validated in the assay of your choosing. Please contact us to learn more about these and other service offerings
|Peptide antigen design and preparation: Peptide synthesis of up to 18 AA residues with purity > 95% Peptide conjugation to KLH carrier protein OR Recombinant protein（offered by customer, >95% pure by SDS Page)||14 7 7|
|One primary intramuscular injection of 50 ug conjugated peptide/protein with adjuvant per mouse One intramuscular injection of 50 ug conjugated peptide/protein with adjuvant per mouse Bleed from tail tips on day 21 post-priming and measure antibody titers by ELISA. Hybridoma fusion from mouse with best titer, positive antigen screen by ELISA of growing clones, followed by 2 rounds subcloning for selected clones including subtype identification and clone amplification||63|
|1 L culture for expression and Protein G purification for one clone. Typical yield of 10-30 mg purified antibody plus hybridoma for delivery to customer||14 7|
|Optional: Application Validation Test WB, IHC, IF, FC||3|
All biopharmaceuticals are immunogenic and may induce anti-drug antibodies (ADAs). The clinical effects of ADA formation can be highly variable and may cause severe adverse events that put the patient at risk. When designed correctly, high affinity ADAs can be ideal for use in pharmacokinetic bridging ELISAs and as reference standards in ADA assays. It is therefore imperative to develop ADAs with the appropriate sensitivity, specificity, and selectivity to guide biotherapeutic drug development decision-making.
Abcepta provides a fast and customer-oriented custom ADA generation service to support the clinical development of biotherapeutic drugs. Our approach is to first evaluate the customer’s most critical factors for each individual project, and then present tailored protocols and project management for approval. Allow Abcepta to be a part of your team!
ABCEPTA specializes in antibody production and has extensive experience
in the development of anti-drug antibodies, including development of anti-idiotype antibodies.
ABCEPTA CASE STUDY: Monoclonal Anti-idiotype Antibodies
The concentration of Anti-ID Ab is 37ng/mI, and the concentration
of negative controls (HuIgG1k) is 1 ug/mI
(Data from customer)
Anti-ID Ab initial concentration is 1ug/mL. Anti-ID Ab binds specifically to
idiotype Ab, but not other negative controls (HuIgG1k).
Phospho-specific antibodies are critical tools used for research of signaling pathways and regulatory networks in various cellular processes. Abcepta has vast experience in the development of custom and off the shelf antibodies against specific phosphorylated residues in different protein targets.
Additionally, Abcepta can also develop polyclonal /monoclonal antibodies against methylated and acetylated residues, valuable tools in epigenetics research.
Our project managers will work with you from antigen design to validation.
|Antigen design and preparation: Modified peptide synthesis Peptide conjugation to KLH||7 14|
|Control non modified peptide synthesis Peptide conjugation to KLH||7 14|
|Animal immunization: rabbits for polyclonal and mouse for monoclonal||1|
|Test bleed ELISA screening for phosphopeptide, non-phosphopeptide||35-49|
|Hybridoma fusion and screening for phosphopeptide, non-phosphopeptide clones, clones amplification and storage||50|
|Two step phosho-specific purification by peptide affinity||99|
|Validation test by WB (limited guarantee available)||3|
|Validation test by IF (limited guarantee available)||3|
|Validation by other tests (limited guarantee available)||3-10|
Abcepta is a leading provider of high-quality peptide synthesis services. Based on decades of experience, Abcepta peptide chemists craft optimized coupling reactions, carefully select building blocks and resins, deploy case-specific methods to circumvent hydrophobic interactions, and devise backup routes in the event of synthetic difficulties. We consult with you during project design, at critical project junctures, and post-delivery with free lifetime technical support. We take your custom peptide expectations personally, and we are relentless in delivering satisfaction on every aspect of your project.
|Peptide Form||Single peptides|
|Peptide Scale||From milligram to multi-gram scale|
|Peptide Purification||HPLC purification up to 98% (Crude peptide also available)|
|Peptide Length||Routine synthesis of peptides ≤ 30 aa. Long peptide synthesis using special chemistry (40-100 aa)|
|Modifications||Phosphorylation, methylation, acetylation, biotinylation, MAPS (4 or 8 chalns). Please inquire for other modification|
|Labels||FITC, rhodamine, TAMRA. Please inquire for other labels|
|Conjugations||Keyhole limpet hemocyanin (KLH), BSA, Hydralink|
|Analysis||Liquid chromatography, mass spectrometry (LC/MS)|
|Libraries||Customized. Please inquire|
|Allquoting||Order your peptides pre-allquoted for greater convenience.|