Product Categories

SPIN-COLUMN-BASED PROTEIN EXTRACTION / CELL FRACTIONATION

Invent Biotechnologies, founded in 2010, is renowned globally for revolutionizing protein extraction and cell fractionation. We have developed innovative spin column-based technologies for protein research that simplify and enhance sample preparation. This results in smoother workflows and consistently reliable outcomes, leading to superior data quality. Researchers who opt for our Minute™ Kits for protein extraction and cell fractionation can trust that their samples accurately represent the natural characteristics of the cells or tissues being studied. These kits only require a benchtop centrifuge, minimizing experimental variables and ensuring high-quality materials for more dependable data. The effectiveness of the Minute™ approach is supported by an increasing number of publications, including mentions in prestigious scientific journals such as Nature, Science, and Cell.

The Minute™ Benefits:

• Super-fast (as quick as 1 minute)
• Small sample size
• Minimized cross contaminations
• Unaltered endogenous baseline
• All living organism and tissue types

The Minute™ Applications:

Protein trafficking/translocation
Protein-protein interaction
Protein modification
Signal transduction
sc/snRNA-seq

Products

Rapid Protein Extraction Kits

Why do we call them Minute™ Kits? Because you can get high-quality, total protein extracts in just minutes! (Yes, sometimes as little as one minute.)

Avoid the problem of RIPA-insoluble proteins and obtain a more complete protein profile using a very simple spin column-based method. Once you experience the combination of the streamlined workflow and superior protein profile, you’ll never look back. Try them!

Description	Catalog No.
Minute™ Total Protein Extraction Kit for Animal Cultured Cells and Tissues (50 Preps)	SD-001/SN-002
Minute™ Detergent-Free Protein Extraction Kit for Animal Tissues and Cultured Cells(50 Preps)	SN-006
Minute™ Total Protein Extraction Kit for Plant Tissues (50 Preps)	SD-008/SN-009
Minute™ Detergent-Free Plant Protein Extraction Kit (50 Preps)	SN-010
Minute™ Total Protein Extraction Kit for Mass Spectrometry (50 Preps)	MS-026
Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes (20 Preps)	AT-022
Minute™ Bacterial Total Protein Extraction Kit (50 Preps)	SB-004
Minute™ Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Preps)	YD-016
Protein Extraction Kit for Hair and Nails (20 Preps)	HD-021
Minute™ Protein Extraction Kit for Fixed and Embedded Tissues (20 Preps)	FE-025
Minute™ Total Protein Extraction Kit for Skin Tissue (50 Preps)	SA-01-SK
Minute™ Total Protein Extraction Kit for Bone Tissue (50 Preps)	SA-02-BT
Minute™ Total Protein Extraction Kit for Blood Vessels (50 Preps)	SA-03-BV
Minute™ Total Protein Extraction Kit for Tendons (50 Preps)	SA-04-TD
Minute™ Total Protein Extraction Kit for Intestines and Mesentery (50 Preps)	SA-05-IM
Minute™ Total Protein Extraction Kit for Muscles (50 Preps)	SA-06-MS
Minute™ Total Protein Extraction Kit for Insects (50 Preps)	SA-07-IS
Minute™ Histone/DNA Binding Protein Extraction Kit (50 Preps)	HP-014
Minute™ Protein/Nucleic Acid Extraction Kit from Gels (20 Preps)	PN-019
Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Preps)	YT-015

Cell Fractionation / Protein Trafficking

A key tool in understanding protein trafficking is subcellular fractionation. Traditionally, this has been performed by sucrose gradient ultracentrifugation. Unfortunately, not every lab has an ultracentrifuge readily accessible to it.

Invent Biotechnologies Minute™ subcellular fractionation kits eliminate the need for ultracentrifugation and make the entire protocol much easier. Following simple, well-defined protocols and using a convenient spin column format, you can obtain preparations of various organelles or other subcellular fractions in under an hour!

Description	Catalog No.
Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit (50 Preps)	SM-005
Minute™ Lysosome Isolation Kit for Mammalian Cells/Tissues (20 Preps)	LY-034
Minute™ Endosome Isolation and Cell Fractionation Kit (20 Preps)	ED-028
Minute™ Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues (20 Preps)	NT-032
Minute™ ER Enrichment Kit (20 Preps)	ER-036
Minute™ Cytoplasmic & Nuclear Extraction Kits for Cells (50 Preps)	SC-003
Minute™ Golgi Apparatus Enrichment Kit (20 Preps)	GO-037
Minute™ Chloroplast Isolation Kit (50 Preps)	CP-011
Minute™ Plasma Membrane-Derived Lipid Raft Isolation Kit (20 preps)	LR-042
Minute™ Plant Cytosolic and Nuclear Protein Isolation Kit (Need 150 mg Raw Material, 20 preps)	PF-045
Minute™ Plant Plasma Membrane Protein Isolation Kit (50 Preps)	SM-005-P
Minute™ Total Lipid Raft Isolation Kit for Mammalian Cells/Tissues	LR-039
Minute™ Mitochondria Isolation Kit for Mammalian Cells and Tissues (50 Preps)	MP-007
Minute™ Nuclear Envelope Protein Extraction Kit (50 Preps)	NE-013
Minute™ High-Efficiency Exosome Precipitation Reagent (20 ml)	EI-027
Minute™ Adipose Tissue Fractionation Kit (20 Preps)	AF-023
Minute™ Plant Microsomal Membrane Extraction Kit (50 Preps)	MM-018
Minute™ High Fidelity ER Isolation Kit for Liver Tissue (20 Preps)	ER-035
Minute™ Synaptosome Isolation Kit (50 preps)	SY-052
Minute™ Mitochondria Isolation Kit for Muscle Tissues/Cultured Muscle Cells (50 preps)	MM-038
Minute™ Detergent-Free Nuclear Matrix Protein Extraction Kit (For Mammalian Cultured Cells/Tissues) (20 Preps)	NM-033
Minute™ Plant ER Enrichment Kit (20 preps)	PR-048
Minute™ Plant Golgi Apparatus Enrichment Kit (20 preps)	PG-049
Minute™ Yeast Mitochondria Enrichment Kit (50 Preps)	YM-017
Minute™ Thylakoid Enrichment and Chloroplast Fractionation Kit (20 preps)	CF-055
Minute™ Plant Endosome Enrichment Kit (20 preps)	PE-050
Minute™ Cytosolic Proteasome Enrichment Kit (20 preps)	PT-040
Minute™ Nuclear Proteasome Enrichment Kit (20 preps)	PN-041
Minute™ Plant Lipid Raft Isolation Kit (20 preps)	PL-051
Minute™ High-Efficiency Saliva Exosome Isolation Kit (50 Preps)	SE-030
Minute™ Chloroplast Isolation kit for Mucilaginous Plants (20 preps)	CF-053

Cell Isolation Kits

Cell isolation protocols do not need to be tedious. We have developed the Minute™ Single Cell Isolation Kits to provide an easy, fast, spin column-based method. The combination of chemical and physical separation mechanisms leads to vastly improved results without requiring specialized instrumentation. Obtain a cell suspension with high integrity and viability in just minutes!

Description	Catalog No.
Minute™ Single Cell Isolation Kit for Fresh and Fixed Tissues (50 Preps)	SC-012
Minute™ Cell Suspension Isolation Kit from Fresh/Frozen Tissues (50 Preps)	CS-031

Single Nuclei Isolation

Get intact, purified nuclei in just minutes from tissue or cells! Invent Biotechnologies’ Minute™ Single Nuclei Isolation Kits are meant for the biologist who cannot trade speed for quality (or vice versa!) We have created one of the fastest, high-quality, easy-to-use, spin-column-based methods for nuclei isolation. Minute™ Single Nuclei Isolation Kits are great for snRNA-seq, ATAC-seq, FACS, and much more.

Description	Catalog No.
Minute™ Detergent-Free Single Nuclei Isolation Kit (20 Preps)	NI-024
Minute™ Single Nucleus Isolation Kit for Neuronal Tissues/Cells (20 preps)	BN-020
Minute™ Single Nucleus Isolation Kit for Tissues/Cells	SN-047
Minute™ Single Nuclei and Cytosol Isolation Kit for Adipose Tissues/Cultured Adipocytes (20 preps)	AN-029
Minute™ Anti-Clumping Nuclei Storage Buffer (10 ml)	WA-014
Minute™ Single Nuclei Isolation Kit for Plant Tissues (20 preps)	PS-054

Specialty Kits

Description	Catalog No.
Minute™ Rubisco Depletion Kit	RD-046
Minute™ Native Protein Precipitation Kit from Saliva (20 preps)	SV-043
Minute™ Organic Solvent-Free Milk Lipid Depletion Kit	ML-044

Protein Analysis Reagents

Our protein analysis reagents compliment our Minute™ Rapid Protein Extraction Kits, enhancing your protein and peptide research. We have reagents for protein solubilization, protein precipitation, western blotting, immunoassay enhancement, and more.

Description	Catalog No.
Minute™ Antibody Enhancer (30 ml)	WA-002
Minute™ Western Blot Stripping Solution (250 ml)	WA-003
Minute™ Ponceau S Stain (250 ml)	WA-004
Minute™ Peroxidase Suppressor (30 ml)	WA-005
Minute™ High-Efficiency Protein Precipitation Kit (30 ml)	WA-006
Minute™ RBC Lysis Buffer (250 ml)	WA-007
Minute™ WB Blocking Solution (250 ml)	WA-008
Minute™ Denaturing Protein Solubilization Reagent (10 ml)	WA-009
Minute™ Non-Denatured Protein Solubilization Reagent (10 ml)	WA-010
Minute™ Protein Solubilization Reagent for MS (2 ml)	WA-011
Minute™ Albumin Depletion Reagent for Plasma and Serum (20 ml)	WA-013
Minute™ Anti-Clumping Nuclei Storage Buffer (10 ml)	WA-014