Please use the following link to place an order and specify your target gene; 

Drug discovery and development of therapeutic approaches relies heavily on the association of genotypes with phenotypes. One of the best ways to carry out this strategy is to disrupt gene function and then analyze changes in the phenotype. Using RNAi and CRISPR biological tools, researchers can study gene function by suppressing gene expression at the translational or genetic level, respectively. However, both systems have certain limitations.

Directly regulating the expression of endogenous genes by targeting DNA offers several advantages compared with oligodeoxynucleotides (ODNs) or RNA interference (RNAi) approaches to down-regulate gene expression. For downregulation of endogenous genes directly at the DNA level, efficiency is likely to increase as only two copies of DNA per cell need to be targeted compared to the thousands of mRNAs that are usually required to be targeted in RNAi approaches.

CRISPRi system also necessitates utilization of large plasmids, technical experience and long optimization processes. Suppression of endogenous gene expression using specific EndoGenius Suppressor Kit results in effective inhibition of all splice variants that is expressed in that specific cell or tissue.

Utilization of EndoGenius Suppressor Kit allows inhibition of specific gene expression with minimal off-target effects. It is quite easy to carry out an assay to see functional effects of suppressing an endogenous gene.

It is also possible with EndoGenius Suppressor to target different genes of a gene family simultaneously. Therefore, the expression of multiple genes can also be easily altered using a single tool.

EcoScript 5x First Strand cDNA Synthesis Kit offer whole components for cDNA synthesis in one solution for the effective synthesis of first strand cDNA from RNA templates. EcoScript 5x First Strand cDNA Synthesis Kit utilizes the recombinant thermostable M-MuLV Reverse Transcriptase which exhibits lower RNase H activity than AMV reverse transcriptase.

EcoScript 5x First Strand cDNA Synthesis Kit already include a thermostable M-MuLV Reverse Transcriptase, RNase Inhibitor, Random primers, Oligo dT Primer, dNTP Mixture, and Reaction Buffer. Only water and RNA template is added to set up the reaction.

ClearPeak 2x Probe Master Mix is a real-time fluorescence quantitative PCR premix for probe method with a concentration of 2×. ClearPeak 2x Probe Master Mix contains EcoTaq DNA Polymerase, PCR buffer, dNTPs, Mg2+,

enhancers, and stabilizers. All you need to do is add templates, primers, and probes, making the operation simple. ClearPeak 2x Probe Master Mix has a dual antibody modified hot start DNA polymerase with a polymerase activity blocking rate of over 95% at temperatures up to 55°C, effectively reducing non-specific amplification at low temperatures. Simultaneously, the combination of a unique PCR buffer system significantly improves the efficiency of qPCR amplification.

Fast: PCR lasts less than competitors. ClearPeak 2x Probe Master Mix saves your time.

Ecological: Your PCR wastes less electricity since it lasts shorter. ClearPeak 2x Probe Master Mix helps protecting environment.

ClearPeak 2x SYBR Master Mix is a specialized premix for real-time fluorescence quantitative qPCR reactions using the dye method (SYBR Green I). The core component EcoTaq DNA Polymerase is an antibody based hot start DNA polymerase that can be restored by heating at 95°C for 5 seconds. It has many advantages such as strong specificity and high detection sensitivity, and is paired with an optimal buffer optimized for qPCR. The unique qPCR buffer system of this product, combined with hot start enzymes, effectively inhibits the production of non-specific products and significantly improves the amplification efficiency of qPCR. It is very suitable for high specificity and sensitivity qPCR reactions. This product is also suitable for qPCR rapid reaction program. Good standard curves can be obtained within a wide quantitative range, with accurate, reproducible, and reliable quantification of target genes.

Fast: PCR lasts less than competitors. ClearPeak 2x SYBR Master Mix saves your time.

Ecological: Your PCR wastes less electricity since it lasts shorter. ClearPeak 2x SYBR Master Mix helps protecting environment.

ClearPeak SYBR Green qPCR Assays contains primer pairs designed for real-time PCR using intercalating dyes, such as ClearPeak 2x SYBR Master Mix. ClearPeak SYBR Green qPCR Assays are pre-designed and pre-validated prior to delivery to users.

ClearPeak Gene Expression Assays (FAM) contains primer and probe sets designed for quantitative gene expression studies utilizing hybridization probes with ClearPeak 2x Probe Master Mix. ClearPeak Gene Expression Assays (FAM) are pre-designed and pre-validated prior to delivery to users.

ClearBand TRUEzol Reagent is a ready-to-use reagent composed of phenol and a mixture of other components for the isolation of high-quality total RNA from various biological materials including animal and plant tissues, cells and bacteria.

Biological materials are homogenized or lysed in ClearBand TRUEzol Reagent and then separated into three phases: a clear upper aqueous phase with the RNA, a pink lower organic phase and an interphase, containing DNA and protein. RNA is purified by precipitation with isopropyl alcohol. And then washed to remove impurities.

Purified RNA can be effectively used in routine downstream applications including cDNA synthesis, northern blotting, differential display, primer extension, and mRNA selection.

ClearBand RNAintact, RNA stabilization solution is prepared using molecular biology grade reagents in ultrapure water. ClearBand RNAintact penetrates tissues and cells rapidly and allows stabilization and protection of cellular RNA in unfrozen fresh specimens at room temperature.
ClearBand RNAintact does not necessitate immediate processing of samples for RNA isolation or freezing of samples in liquid nitrogen for further processing.
The integrity of the RNA content of the cells and tissue pieces that are immediately immersed in ClearBand RNAintact is maintained. Samples immersed within ClearBand RNAintact can be stored up to 1 day at 37°C, 1 week at 25°C, and 1 month at 4°C without a significant loss in RNA integrity. Tissues can also be stored at -20°C or -80°C for long-term.
ClearBand RNAintact is compatible with various downstream applications like total RNA isolation using either TRIzol reagent or spin column-based kits.

EcoPURE Total RNA Kit is designed as a simple and convenient purification of high quality total RNA including small RNAs (e.g. microRNAs) from whole blood, cultured cells, and frozen or fresh tissues. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, β-mercaptoethanol, or time-consuming alcohol precipitation. The standard protocol lasts less than 10 minutes at room temperature and purified RNA can be effectively used in routine downstream applications including cDNA synthesis, northern blotting, differential display, primer extension, and mRNA selection.

EcoPURE Total RNA Kit has been cited in Bilgic SN, Domaniku A, Toledo B, Agca S, Weber BZC, Arabaci DH, Ozornek Z, Lause P, Thissen JP, Loumaye A, Kir S. EDA2R–NIK signalling promotes muscle atrophy linked to cancer cachexia. Nature (2023).

EcoSpin Plant Total RNA Kit is designed as a simple and convenient purification of high- quality total RNA from up to 300 mg of plant material. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, β- mercaptoethanol, or time-consuming alcohol precipitation. The standard protocol lasts less than 40 minutes at room temperature and purified RNA can be effectively used in routine downstream applications including cDNA synthesis, northern blotting, differential display, primer extension, and mRNA selection.

EcoSpin FFPE Total RNA Kit is designed as a simple and convenient purification of total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue materials. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, β-mercaptoethanol, or time-consuming alcohol precipitation. The standard protocol lasts less than 40 minutes at room temperature and purified RNA can be effectively used in routine downstream applications.

EcoSpin FFPE Genomic DNA Kit is designed as a simple and convenient purification of genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue materials. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard protocol lasts approximately 5 hours at room temperature and purified DNA can be effectively used in routine downstream applications.

EcoPURE PCR/Gel Purification Kit combines 2 kits in 1. It is designed for effective and fast purification of polymerase chain reaction (PCR) products. Using this kit, primer dimers, free nucleotides in the reaction, salts, and Taq polymerase can be easily removed. This kit is also suitable for purification of nucleic acids from reactions including restriction digestion, alkaline phosphatase treatment, or kinase reactions.

EcoPURE Bacterial/Yeast/Fungi Genomic DNA Kit is designed for a simple and convenient purification of high quality genomic DNA from Gram (-) negative and Gram (+) positive bacterial cells, yeast, and fungi. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard protocol lasts less than 25 minutes and purified DNA can be used directly in PCR, qPCR, sequencing and enzymatic reactions.

EcoSpin Plasmid Isolation Kit is designed as a simple, convenient, and cost-effective purification of high quality plasmid DNA from recombinant E. coli cultures. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for time-consuming alcohol precipitation method. The standard protocol lasts less than 25 minutes and yields up to 20 µg of plasmid DNA. The kit can be effectively used for purification of any size plasmids and cosmids. The relative plasmid yield and optimal culture size depend on the plasmid copy number and medium used for the bacterial culture.

EcoSpin Gel Purification Kit is designed for effective and fast purification of polymerase chain reaction (PCR) products. Using this kit, primer dimers, free nucleotides in the reaction, salts, and Taq polymerase can be easily and effectively removed.

EcoSpin PCR Purification Kit is designed for effective and fast purification of polymerase chain reaction (PCR) products. Using this kit, primer dimers, free nucleaotides in the reaction, salts, and Taq polymerase can be easily removed. This kit is also suitable for purification of nucleic acids from reactions including restriction digestion, alkaline phosphatase treatment, or kinase reactions.

EcoSpin Yeast Genomic DNA Kit is designed as a simple and convenient purification of high quality genomic DNA from yeast cells. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard protocol lasts less than 25 minutes and purified DNA can be used directly in PCR, qPCR, sequencing and enzymatic reactions.

EcoSpin Insect Genomic DNA Kit is designed as a simple and convenient purification of high quality genomic DNA from 30-50 mg insects. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard protocol lasts less than 30 minutes and purified DNA can be used directly in PCR, qPCR, sequencing and enzymatic reactions.

EcoSpin Bacterial Genomic DNA Kit is designed as a simple and convenient purification of high quality genomic DNA from Gram (-) negative and Gram (+) positive bacterial cells. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard protocol lasts less than 25 minutes and purified DNA can be used directly in PCR, qPCR, sequencing and enzymatic reactions.

EcoSpin Blood Genomic DNA Kit is is designed as a simple and convenient purification of high quality genomic DNA from whole blood. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard protocol lasts less than 25 minutes and purified DNA can be used directly in PCR, qPCR, Southern blotting and enzymatic reactions.

EcoSpin Tissue Genomic DNA Kit is designed as a simple and convenient purification of high quality genomic DNA from fresh or frozen tissue samples and rodent tails. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard protocol lasts less than 30 minutes and purified DNA can be used directly in PCR, qPCR, sequencing and enzymatic reactions.

EcoSpin Cell Genomic DNA Kit is designed as a simple and convenient purification of high quality genomic DNA from cultured cells. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard protocol lasts less than 25 minutes and purified DNA can be used directly in PCR, qPCR, sequencing and enzymatic reactions.

ClearBand Potassium Acetate, 3M pH:5.5 Ready-to-use Solution is prepared using molecular biology grade potassium acetate in ultrapure water. ClearBand Potassium Acetate, 3M pH:5.5 Ready-to-use Solution is 0.22 µm filter sterilized and suitable for purification and precipitation of nucleic acids. It can be used for also precipitating dodecyl sulfate (DS) and DS-bound proteins allowing the removal of proteins from DNA.

ClearBand 1x Red Blood Cell Lysis Buffer is prepared using molecular biology grade ammonium chloride in ultrapure water. It is formulated for effective and quick lysis of red blood cells with little to no effect on leukocytes.

ClearBand RNAintact, RNA stabilization solution is prepared using molecular biology grade reagents in ultrapure water. ClearBand RNAintact penetrates tissues and cells rapidly and allows stabilization and protection of cellular RNA in unfrozen fresh specimens at room temperature.
ClearBand RNAintact does not necessitate immediate processing of samples for RNA isolation or freezing of samples in liquid nitrogen for further processing.
The integrity of the RNA content of the cells and tissue pieces that are immediately immersed in ClearBand RNAintact is maintained. Samples immersed within ClearBand RNAintact can be stored up to 1 day at 37°C, 1 week at 25°C, and 1 month at 4°C without a significant loss in RNA integrity. Tissues can also be stored at -20°C or -80°C for long-term.
ClearBand RNAintact is compatible with various downstream applications like total RNA isolation using either TRIzol reagent or spin column-based kits.

ClearBand 10x Red Blood Cell Lysis Buffer is prepared using molecular biology grade ammonium chloride in ultrapure water. It is formulated for effective and quick lysis of red blood cells with little to no effect on leukocytes. ClearBand10x Red Blood Cell Lysis Buffer should be diluted in double-distilled water prior to use.

ClearBand TRUEzol Reagent is a ready-to-use reagent composed of phenol and a mixture of other components for the isolation of high-quality total RNA from various biological materials including animal and plant tissues, cells and bacteria.

Biological materials are homogenized or lysed in ClearBand TRUEzol Reagent and then separated into three phases: a clear upper aqueous phase with the RNA, a pink lower organic phase and an interphase, containing DNA and protein. RNA is purified by precipitation with isopropyl alcohol. And then washed to remove impurities.

Purified RNA can be effectively used in routine downstream applications including cDNA synthesis, northern blotting, differential display, primer extension, and mRNA selection.

ClearBand DNA Marker 100bp-Green is a ready-to-load molecular weight marker suitable for DNA fragment size determination on gel electrophoresis.

PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 12 bands, ranging from 100 bp – 3 kb, for molecular weight standards in agarose gel electrophoresis. The 500 and 1,500 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.

ClearBand SAFE DNA Gel Stain Solution, 20,000x, is an aqueous fluorescent nucleic acid gel stain as a safer alternative to traditional ethidium bromide (EtBr) to be used at 1x concentration in agarose or polyacrylamide gels.

One vial (1 ml) of ClearBand SAFE DNA Gel Stain Solution, 20,000x, can be used to prepare at least 200 precast mini gels. ClearBand SAFE DNA Gel Stain Solution, 20,000x is compatible with downstream applications including gel extraction and cloning.

Key Features;

Safety: No toxicity, no mutagenicity and no carcinogenicity.

Eco-friendly: Safe to dispose in the drain. No hazard wastes.

Affordable: Low cost. An affordable alternative to competing dyes.

Compatibility: Compatible with instruments that are suitable for detection of gels with EtBr. Suitable for blue LED detection.

ClearBand 6x DNA Loading Dye Green is a pre-mixed buffer, which can be used for loading DNA markers and samples into the wells of agarose and non-denaturing polyacrylamide gels.It contains two tracking dyes, Xylene Cyanol FF and Orange G, for visualization of DNA migration on the gels. Orange G runs around 50 bp fragment in agarose gels, which provides much better tracking of DNA before it runs off the gel. Besides, Orange G does not result in formation of a dark shadow on the gel as Bromophenol Blue does, which runs around 400 bp fragment precisely where PCR fragments are likely to be.

ClearBand 6x DNA Loading Dye Blue is a pre-mixed buffer, which can be used for loading DNA markers and samples into the wells of agarose and non-denaturing polyacrylamide gels.

It contains two tracking dyes, Xylene Cyanol FF and Bromophenol Blue, for visualization of DNA migration on the gels. Xylene Cyanol FF runs around 400 bp and Bromophenol Blue runs around 400 bp fragment in agarose gels, which provides a feasible tracking of DNA before it runs off the gel.

ClearBand TBE 10x Electrophoresis Buffer is a commonly used concentrate buffer for separation of nucleic acids in agarose and polyacrylamide gels. It should be diluted to a working concentration of 1x before use. Fresh 1X buffer is recommended to use for each electrophoresis application.

ClearBand TBE 10x Electrophoresis Buffer is filter sterilized and prepared with ultra pure water.

1x Buffer Composition: 90 mM Tris, 90 mM boric acid, 2 mM EDTA.

ClearBand TAE 50x Electrophoresis Buffer is a commonly used concentrate buffer for separation of nucleic acids in agarose and polyacrylamide gels. It is preferred in high resolution agarose gel electrophoresis applications and separation of high molecular weight, double-stranded DNA. It should be diluted to a working concentration of 1x before use. Fresh 1X buffer is recommended to use for each electrophoresis application.

ClearBand TAE 50x Electrophoresis Buffer is filter sterilized and prepared with ultra pure water.

1x Buffer Composition: 40 mM Tris, 20 mM acetic acid, 1 mM EDTA.

ClearBand Lyse&Load is a premixed buffer, which combines RIPA Lysis Buffer and Laemmli Sample Buffer in one solution for protein sample preparation prior to loading on SDS-PAGE. 25 µl Bmercaptoethanol should be added per 1 ml ClearBand Lyse&Load prior to use.

ClearBand  Prestained Protein Marker is a protein standard containing 12 pre-stained proteins covering a wide molecular weight range from 10 to 245 kDa.

Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). This protein marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for approximating the size of proteins. The protein marker is supplied in gel loading buffer and is ready to use.

ClearBand Laemmli Sample Buffer 2x is a premixed buffer for protein sample preparation prior to loading on SDS-PAGE. It is supplied as 2x concentrate. 50 µl B-mercaptoethanol should be added per 950 µl ClearBand Laemmli Sample Buffer 2x prior to use.

ClearBand Laemmli Sample Buffer 4x is a premixed buffer for protein sample preparation prior to loading on SDS-PAGE. It is supplied as 4x concentrate. 100 μl B-mercaptoethanol should be added per 900 μl ClearBand Laemmli Sample Buffer 4x prior to use.

ClearBand Laemmli Sample Buffer 10x is a premixed buffer for protein sample preparation prior to loading on SDS-PAGE. It is supplied as 10x concentrate. 20 µl B-mercaptoethanol should be added per 80 µl ClearBandLaemmli Sample Buffer 10x prior to use.

ClearBand Nitrocellulose Membrane is dense 100% nitrocellulose combining the advantages of high protein binding capacity with low background and high membrane stability, which ensures easy handling and excellent signal-to-noise results. ClearBand Nitrocellulose Membrane has excellent binding properties for western blotting, dot-blot assays, and other protein or nucleic acid methods. They are used for a wide range of molecular weight proteins and nucleic acids >300 bp.

ClearBand Polyvinylidene Fluoride (PVDF) membranes display good mechanical strength, strong protein retention, low background, extensive solvent compatibility, and excellent coloring ability. Exceptional strength, high binding capacity and chemical compatibility of ClearBand PVDF membrane make it ideal for use in Western blots, immunoblotting, and solid phase assays and plaque lifts.

ClearBand PVDF membranes are highly hydrophobic and must be pre-wetted with methanol prior to submersion in transfer buffer. It has exceptional tensile strength, preventing it from cracking, tearing, breaking or curling.

ClearBand ECL Western Blotting Substrate is specifically formulated for highly sensitive, non-radioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. ClearBand ECL Western Blotting Substrate offers excellent signal to noise ratio and clear background. ECL50 is sufficient for 400 cm²of membrane contents and ECL250 is sufficient for 2000 cm²of membrane contents.

ClearBand Re-Blot Stripping Solution is a uniquely formulated, ready-to-use reagent prepared for safely and effectively removing primary and secondary antibodies from nitrocellulose and PVDF membranes to allow use the same membrane for different antibodies. It helps scientists save their time and budgets without damaging the target antigen during stripping.

Applications

• Reuse a nitrocellulose or PVDF membrane to detect different targets using different antibodies in the same membrane
• Reprobe a membrane to optimize antibody concentrations that were ineffective the first time

ClearBand Ponceau S is a ready to use solution for the rapid (5 min) and reversible detection of protein bands on nitrocellulose, PVDF, cellulose acetate and membranes. ClearBand Ponceau S rapidly stains proteins on membranes pink or light red.

This staining solution is generally used to confirm protein transfer in Western blotting applications before probing with select antibodies.

ClearBand Ponceau S does not have a deleterious effect on the blotted polypeptides and can be completely removed from the membranes by repeated wash.

ClearBand Bradford Reagent is formulated for a ready-to-use total protein analysis reagent used for quick measurement of total protein concentration. ClearBand Bradford Reagent contains Coomassie Brilliant Blue G-250, which associates with basic and aromatic amino acids, thus leading to a shift in absorbance during protein determination.ClearBand Bradford Reagent offer an easy-to-use assay in either test tube or microplate format: mix protein sample with the assay reagent, incubate shortly and measure the absorbance at 595nm. Color response with Coomassie is non-linear with increasing protein concentration, therefore, a standard curve must be created with each assay.

ClearBand RIPA Lysis Buffer is formulated for efficient and complete cell lysis and solubilization of proteins. ClearBand RIPA Lysis Buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins and is compatible with several applications, including reporter assays, protein assays, immunoassays and protein purification.

ClearBand 10x RIPA Lysis Buffer is formulated for efficient and complete cell lysis and solubilization of proteins. ClearBand 10x RIPA Lysis Buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins and is compatible with several applications including western blotting and immunoprecipitation.

ClearBand 1M Tris, pH 6.8 is a pre-mixed and pH-adjusted ready to use sterile-filtered solution. ClearBand 1M Tris, pH 6.8 can be diluted to desired concentration and can be used in molecular biology or general biochemistry applications.

ClearBand 1M Tris, pH 7.4 is a pre-mixed and pH-adjusted ready to use sterile-filtered solution. ClearBand 1M Tris, pH 7.4 can be diluted to desired concentration and can be used in molecular biology or general biochemistry applications.

ClearBand 1M Tris, pH 8 is a pre-mixed and pH-adjusted ready to use sterile-filtered solution. ClearBand 1M Tris, pH 8 can be diluted to desired concentration and can be used in molecular biology or general biochemistry applications.

ClearBand 1M Tris, pH 8.5 is a pre-mixed and pH-adjusted ready to use sterile-filtered solution. ClearBand 1M Tris, pH 8.5 can be diluted to desired concentration and can be used in molecular biology or general biochemistry applications.

ClearBand 1M Tris, pH 8.8 is a pre-mixed and pH-adjusted ready to use sterile-filtered solution. ClearBand 1M Tris, pH 8.8 can be diluted to desired concentration and can be used in molecular biology or general biochemistry applications.

ClearBand 10x Tris/Glycine/SDS Running Buffer, pH:8.3, is formulated for separation of proteins in the denatured form on sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE).

ClearBand 10x Tris/Glycine/SDS Running Buffer provides a convenient way to ensure high-quality, consistent, and reproducible electrophoresis results.

ClearBand 10x Tris/Glycine/SDS Running Buffer is filter sterilized and prepared with ultra pure water.

ClearBand Acrylamide/Bisacrylamide, 37.5:1 %30 Ready-to-use Solution is prepared using molecular biology grade acrylamide and bisacrylamide in ultrapure water.

ClearBand Acrylamide/Bisacrylamide, 37.5:1 %30 Ready-to-use Solution is 0.22 µm filter sterilized and suitable for electrophoresis of both proteins and nucleic acids. It can be used for preparation of stacking and resolving gels during sodium dodecyl sulfate polyacrylamide gel electrophoresis.

ClearBand Acrylamide/Bisacrylamide, 29:1 %30 Ready-to-use Solution is prepared using molecular biology grade acrylamide and bisacrylamide in ultrapure water.

ClearBand Acrylamide/Bisacrylamide, 29:1 %30 Ready-to-use Solution is 0.22 µm filter sterilized and suitable for electrophoresis of both proteins and nucleic acids. It can be used for preparation of stacking and resolving gels during sodium dodecyl sulfate polyacrylamide gel electrophoresis.

ClearBand Acrylamide/Bisacrylamide, 19:1 %40 Ready-to-use Solution is prepared using molecular biology grade acrylamide and bisacrylamide in ultrapure water.

ClearBand Acrylamide/Bisacrylamide, 19:1 %40 Ready-to-use Solution is 0.22 μm filter sterilized and suitable for electrophoresis of both proteins and nucleic acids. It can be used for preparation of stacking and resolving gels during sodium dodecyl sulfate polyacrylamide gel electrophoresis

ClearBand Tris-Glycine Buffer (10x) is an ideal stock solution for preparing standard Tris-glycine transfer buffer used for Western blotting. ClearBand Tris-Glycine Buffer (10x) is prepared with ultra pure water and filtered sterile.

ClearBand Phosphate-buffered saline (PBS) is a balanced salt solution which is used for a number of cell culture applications including washing cells before trypsinization, transport of cells or tissue samples, diluting cells for counting, and preparing reagents. ClearBand PBS does not contain calcium and magnesium for rinsing chelators from the culture before cell dissociation. ClearBand PBS is prepared with ultra pure water, filtered sterile and autoclaved.

ClearBand 10x Phosphate-Buffered Saline with Tween® 20 (PBST) is a balanced salt solution which is used for especially western blotting and ELISA procedures. ClearBand PBST enables washing without disturbing antibody-antigen binding interactions. ClearBand PBST does not contain calcium and magnesium.

ClearBand 10x PBST should be diluted to 1x working solution. 1x formulation contains 0.05% Tween 20.

ClearBand 10x PBST is prepared with ultra pure water, filter sterilized and autoclaved.

ClearBand 10x Tris-Buffered Saline (TBS) is a balanced pH stabilizing salt solution used for especially western blotting and ELISA procedures.

ClearBand TBS enables washing without disturbing antibodyantigen binding interactions.

ClearBand 10x TBS should be diluted to 1x working solution.

ClearBand 10x TBS is prepared with ultra pure water and filter sterilized.

ClearBand  20x Tris-Buffered Saline (TBS) is a space-saving balanced pH stabilizing salt solution used for especially western blotting and ELISA procedures. ClearBand  TBS enables washing without disturbing antibody-antigen binding interactions.
ClearBand  20x TBS should be diluted to 1x working solution with a pH 7.6±0.1.
ClearBand  20x TBS is prepared with ultra pure water and filter sterilized.

ClearBand 10x Tris-Buffered Saline with Tween® 20 (TBST) is a balanced pH stabilizing salt solution used for especially western blotting and ELISA procedures. ClearBand TBS enables washing without disturbing antibody-antigen binding interactions.

ClearBand 10x TBST should be diluted to 1x working solution. 1x formulation contains 0.1% Tween 20.

ClearBand 10x TBST is prepared with ultra pure water and filter sterilized

ClearBand 20x Tris-Buffered Saline with Tween® 20 (TBST) is a space-saving balanced pH stabilizing salt solution used for especially western blotting and ELISA procedures. ClearBand TBST enables washing without disturbing antibody-antigen binding interactions.

ClearBand 20x TBST should be diluted to 1x working solution. 1x formulation contains 0.1% Tween 20.

ClearBand 20x TBST is prepared with ultra pure water and filter sterilized.

ClearBand Western blot boxes are available in 3 different sizes and will perfectly fit blots of diferent sizes, including whole blots, half blots, and strips.

Save Money on Expensive Antibodies – now less volume is required!

Our ClearBand Western blot boxes significantly help reducing the volume of antibody required while still producing perfect Westerns!

ClearBand Nuclease Free Ultra-Pure Water is prepared under stringent conditions and is suitable for all applications in a molecular biology laboratory. ClearBandNuclease Free Ultra-Pure Water goes through, deionization, reverse osmosis, UV-treatment, 0.2μm filtration, and double autoclave to ensure sterility, being free of nucleases and to completely eliminate the byproducts of DEPC.

NutriCulture Cell Cycle Analysis Kit detects alterations in cell cycle by measuring DNA content. DNA binds to fluorescent dyes like PI and the fluorescence intensity at different stages of the cell cycle detected by flow cytometry can be different, which serves to detect different phases in cell cycle.

NutriCulture Cell Cycle Analysis Kit is intended to be used to detect the DNA content (cell cycle) of cultured suspension or adherent cells.

NutriCulture Fetal Bovine Serum (FBS) is used to supplement basal growth medium in cell culture applications. NutriCulture FBS can provide the cell health, maintenance and viability critical for optimal performance and cell growth in your culture medium.

Heat inactivation of serum is performed by raising the temperature of the serum to 56ºC and maintaining that temperature for 30 minutes.

Dulbecco′s Modified Eagle′s Medium (DMEM) is a standard basal cell culture medium that is a modification of Basal Medium Eagle. NutriCulture DMEM contains four-fold concentrations of the amino acids and vitamins than the original Eagle’s Minimal Essential Medium. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells.
Since then, it has been modified in several ways to support the growth of primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, HEK 293, Cos-7, and PC-12. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name DMEM High Glucose.

Each lot of NutriCulture DMEM High Glucose is prepared from powdered base medium, tissue culture-grade water, and is sterile filtered using a 0.1 micron filter.

Each lot of NutriCulture DMEM High Glucose is tested to confirm the absence of bacterial, fungal, and mycoplasmal contamination.

Dulbecco′s Modified Eagle′s Medium (DMEM) is a standard basal cell culture medium that is a modification of Basal Medium Eagle. NutriCulture DMEM contains four-fold concentrations of the amino acids and vitamins than the original Eagle’s Minimal Essential Medium. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells.

Since then, it has been modified in several ways to support the growth of primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, HEK 293, Cos-7, and PC-12. Each of these media offers a different combination of L-glutamine and sodium pyruvate. The glucose levels have been kept as 1000 mg/L, contributing to the name DMEM Low Glucose.

Each lot of NutriCulture DMEM Low Glucose is prepared from powdered base medium, tissue culture-grade water, and is sterile filtered using a 0.1 micron filter.

Each lot of NutriCulture DMEM Low Glucose is tested to confirm the absence of bacterial, fungal, and mycoplasma contamination.

NutriCulture RPMI 1640, also known as RPMI Medium, was originally developed by Moore and his co-workers in 1966 Roswell Park Memorial Institute to culture human leukemia cells in suspension and monolayer. NutriCulture RPMI 1640 Medium was formulated for use in a 5% carbon dioxide atmosphere and has since been found suitable for culture of a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. NutriCulture RPMI 1640 has also traditionally been used for the serum-free expansion of human lymphoid cells for karyotype analysis.
Each lot of NutriCulture RPMI 1640 is prepared from powdered base medium, tissue culture-grade water, and is sterile filtered using a 0.1 micron filter.
Each lot of NutriCulture RPMI 1640 is tested to confirm the absence of bacterial, fungal, and mycoplasmal contamination.

NutriCulture Cell Viability Detection Kit-8 (CVDK-8) allows very convenient assays by utilizing its highly water-soluble tetrazolium salt. WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. NutriCulture CVDK-8 is a one-bottle solution; no premixing of components is required. NutriCulture CVDK-8, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells. The detection sensitivity using NutriCulture CVDK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.

NutriCulture Trypan Blue, 0.4% Solution is a frequently used reagent to count cells in cell culture facilities when subculturing cells or using them in further functional in vitro and in vivo assays. It is also used to measure cell viability using dye exclusion assay, where only dead cells are stained with NutriCulture Trypan Blue, while viable cells are not stained.

Contamination of cell cultures is a frequent and important problem faced in cell and tissue culture laboratories. It result in delays in experimental schedules, and thus causes waste of precious time, money and efforts.

NutriCulture 100x CellSAFE helps keeping your incubators clear of undesired contamination with its strong antimicrobial and fungicidal features against a wide variety of well-known lab contaminants, without effecting the morphology and proliferative potential of routinely used cells in cell and tissue culture laboratories

NutriCulture DMSO (Dimethyl Sulfoxide), Sterile, is a colorless, polar, aprotic organic solvent used in chemical research. It is miscible with water and many other organic solvents.

NutriCulture DMSO (Dimethyl Sulfoxide), Sterile, is also widely used in polymerase chain reactions (PCR) as a co-solvent, helping to inhibit the formation of secondary structures from DNA fragments and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs via prevention of ice crystal formation during cryopreservation.

NutriCulture Phosphate-Buffered Saline (PBS) is a balanced salt solution, which is used for a number of cell culture applications including washing cells before trypsinization, transport of cells or tissue samples, diluting cells for counting, and preparing reagents. NutriCulture PBS does not contain calcium and magnesium for rinsing chelators from the culture before cell dissociation. NutriCulture PBS is prepared with ultra pure water, filter sterilized and autoclaved.

EcoZyme RNAse A is a pre-made molecular biology grade enzyme solution which is supplied at a concentration of 10 mg/ml in 10 mM Tris-HCl (pH 8). EcoZyme RNAse A from bovine pancreas is a stable and highly reactive, DNase and protease-free, endoribonuclease that specifically degrades single stranded RNA at C and U residues. EcoZyme RNAse A is pretreated to remove DNase I and is suitable for RNA digestion in plasmid and genomic DNA purification procedures.