Cell Lines

A cell line is a permanent in vitro culture of a certain cell type that can continue to create new cells as long as they are given a fresh medium on which to develop. In cellular and molecular biology research, creating a cell line is a very common technique. As compared to standard cell lines, reporter-labeled cell lines, or simply reporter cell lines, provide several uses. Reporter cell lines are existing cell lines to which reporter genes are added to them, enabling the monitoring and observation of protein expression. A vector containing the reporter gene and the desired gene promoter is transfected into a cell line. Green fluorescent protein (GFP), Red fluorescent protein (RFP), Luciferase, Secreted Alkaline Phosphatase (SEAP), and other regularly used reporter genes are visually detectable in life science and biotechnology research.

Abeomics provides a wide range of reporter cell lines, such as NF-kB Leeporter, TLR4/IL-8 Leeporter, NFAT Leeporter, STAT1 Leeporter, and IL-6 Leeporter, to help scientists and researchers in the discovery of novel reporter genes for application in life science and biotechnology research.

Stable cell lines are vital laboratory tools that can be utilized for the expression of a target protein. Stable cell lines might be essential for several research and development programs like the manufacture of therapeutic proteins (including recombinant antibodies), screening of experimental drugs, functional analysis of genes, etc. The principal characteristic of these cell lines is that they are genetically homogenous in culture. These indeterminately replicate and remain to express the transgene constantly throughout its all generations. The development of a stable cell line denotes the method of developing identical populations of cells that establish the expression of a transfected gene insert. Inside the host cell, this transfected gene assimilates into the host cell genome consequently expressing the transfected genetic material. Stable cell lines play an important role in cancer research as it requires the selection of a stable cell so that it will permit the assessment of drug treatments as well as observation for the alterations of cellular behavior to check the efficacy of the drug in vitro.

The stable cell lines’ portfolio of Abeomicscontains several molecules that are involved in immune modulation such as, PD-1 cell line, PD-L1 cell line, PD-L2 cell line, CTLA-4 Cell line, ICOS and ICOSL Cell line, TIM3 cell line, CTLA4 Stable Cell Line, ACE2/HEK293 Stable Cell Line, ACE2/VERO Stable Cell Line, and Vista Stable Cell Line etc.