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Cell-Based Assays

Gene function analysis, target discovery and validation, assay development, and compound screening often need cell-based assays.  Stable cell lines that are engineered to express a gene of interest via transgene integrations into the host genome provide an efficient approach to conduct such analysis.  However, because establishment of such cell lines are tedious in procedures, time-consuming in development process, and expensive in terms of labor, reagents, and materials, as well as post-transfection validation of individual clones, it is always debatable whether is worthy of developing such stable cell lines in individual laboratories.

Signosis now provides a series of gene expression- or luciferase reporter-ready stable cell lines in order to facilitate the studies.

Transcription Factor Assays

Transcription factors (TFs) are DNA binding proteins that play essential roles in regulating gene expression. They act as sensors to monitor changes of cells and convert the signals into changes of gene expression. Often, a specific cellular signal pathway can activate multiple TFs and the expression of a specific gene is under the control of multiple TFs.

Signosis provides a variety of tools to meet a variety of needs and facilitate the analysis of transcription factors. The TF Activation Profiling Plate Arrays, Transcriptional Interaction TF Plate Arrays, and Promoter-Binding TF Profiling Array are for the analysis of multiple TFs, and TF Filter Plate Assays, EMSA Kits, Luciferase Reporter Vectors and Vector Sets, Luciferase Stable Cell Lines, TF ELISA, and TF Western Blot Detection Assay Kits can monitor individual TFs.

Cytokines and Hormones

Cytokines and growth factors are signaling molecules that have critical roles in many biological processes such as cellular growth, differentiation, gene expression, migration, immunity and inflammation. Disruption in these signals can lead to a variety of diseases, including arthritis, liver disease, inflammatory bowel disease, cardiac-related diseases, and cancers.

Signosis provides a number of tools to profile one specific cytokine or growth factor, or to monitor multiple simultaneously.

ChIP Assays

Chromatin immunoprecipitation (ChIP) assay can be used for analyzing the association of a specific TF or co-factor with specific promoters in vivo and providing a snapshot of how a regulatory TF or co-factor affects the expression of a single gene or a variety of genes at the same time.With several improvements, Signosis’ Magnetic-based Snapshot ChIP Assay and Microplate-based Snapshot ChIP Assay are able to efficiently measure the interaction of a specific TF or associated cofactor with target promoters.

miRNA Research

miRNAs are small non-coding RNA molecules that regulate up to 30% of mammalian gene expression. They serve as post-transcriptional regulators and bind to complementary sequences on target messenger RNA transcripts (mRNAs), resulting in translational repression or target degradation. So far, more than 400 miRNAs have been identified in the human genome and many of them are only different in one or a few nucleotides. Dysregulation of miRNA can be associated with many diseases such as cancer; therefore, the research of miRNAs is very important.

Signosis provides a variety of innovative tools for the research of miRNAs including miRBNA arrays for profiling multiple miRNAs simultaneously or monitoring one specific miRNAs.

Gene Expression Analysis

Gene Expression is the process by which information from a gene is used to direct the synthesis of a functional gene product. These products are often proteins. Gene expression analysis is critical to understand the functions of genes and its protein products, as well as to get a clearer picture of the complex regulatory networks that effect fundamental biological processes.

Signosis has a number of assay products for the gene expression study, including the cDNA Plate Arrays for monitoring the expression of genes that are up or down regulated by TFs, and the single cell PCR kits for analyzing gene expression, which allows direct analysis of cell lysates without RNA isolation prior assay.

Protein Interaction Analysis

Protein–protein interactions play important roles in many cellular functions including signaling transduction. Often when two or more proteins bind together, it carries out specific biological functions. Information about these interactions can help uncover the molecular mechanism of cellular regulation, improve the understanding of human diseases, and provide new views of therapeutic approaches.

Signosis provides a number of innovative tools for the research of protein-protein interactions, such as the signaling protein assays and the Co-Immunoprecipitation kits.

Biomarkers & Key Proteins

The presense or altered level of a specific molecule or biomarker can help to confirm a particular disease state. The ability to detect and quantifiably compare levels of disease markers can yield great insight for the study of diseases and the development of treatments.

Signosis provides a variety of ELISA kits to detect biomarkers for autoimmune, cardiac, and tumor diseases. With the tools, certain diseases can be detected more efficiently.

Kinase Pathway Exploration

Kinome represents a small fraction of the human genome (about 2%), however, each of the 518 kinases plays an integral role in many biological processes such as cellular growth, differentiation, gene expression, migration, immunity and inflammation. As signal transducers, kinase activities are tightly regulated to ensure proper cellular functions. Disruption in the activity levels can lead to a variety of diseases, including arthritis, liver disease, inflammatory bowel disease, cardiac-related diseases, and cancers. Therefore, tools to study kinase pathways and activities can improve our understanding of kinases and facilitate drug discoveries.

Signosis provides a number of tools to study kinase activity, or to monitor kinase pathways.