Signosis is a bioassay provider, dedicated to the development and commercialization of plate-based analysis products.

They focus on these important regulatory molecules: cytokines, transcription factors, miRNAs, and disease markers.

Their mission is to become a global supplier of innovative assay products with high sensitivity and cost-effectiveness.


Product Categories

Gene function analysis, target discovery and validation, assay development, and compound screening often need cell-based assays.  Stable cell lines that are engineered to express a gene of interest via transgene integrations into the host genome provide an efficient approach to conduct such analysis.  However, because establishment of such cell lines are tedious in procedures, time-consuming in development process, and expensive in terms of labor, reagents, and materials, as well as post-transfection validation of individual clones, it is always debatable whether is worthy of developing such stable cell lines in individual laboratories.

Signosis now provides a series of gene expression- or luciferase reporter-ready stable cell lines in order to facilitate the studies.

Transcription factors (TFs) are DNA binding proteins that play essential roles in regulating gene expression. They act as sensors to monitor changes of cells and convert the signals into changes of gene expression. Often, a specific cellular signal pathway can activate multiple TFs and the expression of a specific gene is under the control of multiple TFs.

Signosis provides a variety of tools to meet a variety of needs and facilitate the analysis of transcription factors. The TF Activation Profiling Plate Arrays, Transcriptional Interaction TF Plate Arrays, and Promoter-Binding TF Profiling Array are for the analysis of multiple TFs, and TF Filter Plate Assays, EMSA Kits, Luciferase Reporter Vectors and Vector Sets, Luciferase Stable Cell Lines, TF ELISA, and TF Western Blot Detection Assay Kits can monitor individual TFs.

Cytokines and growth factors are signaling molecules that have critical roles in many biological processes such as cellular growth, differentiation, gene expression, migration, immunity and inflammation. Disruption in these signals can lead to a variety of diseases, including arthritis, liver disease, inflammatory bowel disease, cardiac-related diseases, and cancers.

Signosis provides a number of tools to profile one specific cytokine or growth factor, or to monitor multiple simultaneously.

miRNAs are small non-coding RNA molecules that regulate up to 30% of mammalian gene expression. They serve as post-transcriptional regulators and bind to complementary sequences on target messenger RNA transcripts (mRNAs), resulting in translational repression or target degradation. So far, more than 400 miRNAs have been identified in the human genome and many of them are only different in one or a few nucleotides. Dysregulation of miRNA can be associated with many diseases such as cancer; therefore, the research of miRNAs is very important.

Signosis provides a variety of innovative tools for the research of miRNAs including miRBNA arrays for profiling multiple miRNAs simultaneously or monitoring one specific miRNAs.

Gene Expression is the process by which information from a gene is used to direct the synthesis of a functional gene product. These products are often proteins. Gene expression analysis is critical to understand the functions of genes and its protein products, as well as to get a clearer picture of the complex regulatory networks that effect fundamental biological processes.

Signosis has a number of assay products for the gene expression study, including the cDNA Plate Arrays for monitoring the expression of genes that are up or down regulated by TFs, and the single cell PCR kits for analyzing gene expression, which allows direct analysis of cell lysates without RNA isolation prior assay.

The presense or altered level of a specific molecule or biomarker can help to confirm a particular disease state. The ability to detect and quantifiably compare levels of disease markers can yield great insight for the study of diseases and the development of treatments.

Signosis provides a variety of ELISA kits to detect biomarkers for autoimmune, cardiac, and tumor diseases. With the tools, certain diseases can be detected more efficiently.

Kinome represents a small fraction of the human genome (about 2%), however, each of the 518 kinases plays an integral role in many biological processes such as cellular growth, differentiation, gene expression, migration, immunity and inflammation. As signal transducers, kinase activities are tightly regulated to ensure proper cellular functions. Disruption in the activity levels can lead to a variety of diseases, including arthritis, liver disease, inflammatory bowel disease, cardiac-related diseases, and cancers. Therefore, tools to study kinase pathways and activities can improve our understanding of kinases and facilitate drug discoveries.

Signosis provides a number of tools to study kinase activity, or to monitor kinase pathways.

Find out more about Signosis Custom Services below;

By choosing this service you will benefit from;

  1. – Fast and reliable stable cell line generation with minimum turn around time of 8 weeks.
  2. – Strong technical expertise – Our highly experienced scientific team has established 60+ TF luciferase reporter and gene overexpression stable cell lines.
  3. – Excellent customer service – The experienced scientists will consult, discuss  and conduct your projects effectively. The complete functional test is also available upon your request.
  4. – Mycoplasma testing – We guarantee the absence of mycoplasma in generated cell lines.

Signosis provides custom services for making stable cell lines with vectors of your choice or vectors available in the Signosis catalog.  You can also provide your own cell lines or choose from our in-house collection.  The schematic for generating a stable cell line is pictured below and the process takes about 5-8 weeks on average, depending on the complexity of the project.

 

flowchart

miRNA Reporter

Signosis provides cloning services for constructing any miRNA Reporter Vector not listed on our website.

Process

  • To get started, please contact us 
  • If you are interested in the cloning service, please let us know the name or the sequence of the miRNA. If you are interested in the miRNA target reporter vector, please provide the sequence of the UTR region.
  • The project will be initiated by a scientist and completed as soon as we can.

TF Reporter

Signosis provides cloning services for constructing any Transcription Factor Reporter Vector not listed on our website.


Process

  • To get started, please contact us 
  • If you are interested in the cloning service, please let us know the sequence of the Transcription Factor.
  • The project will be initiated by a scientist and completed as soon as we can.

Other DNA Cloning

Signosis provides DNA cloning services and please call or send us an email.

Process

  • To get started, please contact us 
  • If you are interested in the cloning service, please let us know the sequence of the gene of interest.
  • The project will be initiated by a scientist and completed as soon as we can.

TF Profiling Arrays

Two formats of TF Activation Profiling Arrays are available at Signosis.  They measure up to 96 TFs.  If you would like to replace some of the TFs in the arrays with TFs of interest, please call or send an email to us.

Process

To get started, please contact us 

  • If you are interested in the custom TF Profiling Array, please provide us with a list of the TFs you would like to include.
  • The project will be initiated by a scientist and completed as soon as we can.

EMSA

Currently 100+ EMSA Kits for different transcription factors are available at Signosis. Signosis also provides custom probe for any unlisted transcription factors of interest.

Process

To get started, please contact us 

  • Please let us know the DNA binding sequence of TF of interest.
  • The project will be initiated by a scientist and completed as soon as we can.

TF Filter Assays

Currently 100+ filter assay kits for different transcription factors are available at Signosis. Signosis also provides custom services for the development of filter assays for any unlisted transcription factor of interest.


Process

To get started, please contact us 

  • If you are interested in making a custom luciferase reporter vector, please let us know the DNA binding sequence of TF of interest.
  • The project will be initiated by a scientist and completed as soon as we can.

Cytokine ELISA Plate Arrays

Currently Signosis provides cytokine ELISA plate arrays for monitoring up to 64 Human, 39 Mouse, and 16 Rat cytokines. Signosis also provides custom services for the development of an ELISA plate array to include any cytokines of the list. For any cytokines that are not on the list, please call or send us an email.

Human

TNFa VEGF PDGF-BB IL-10
IFNr EGF PIGF-1 FGFb
G-CSF IL-6 b-NGF Leptin
GM-CSF Resistin SCF IGF-1
IL-1a PAI-1 MCP-1 TGFb1
IL-8 IL-12 MIP-1a Adipo
IP-10 IL-13 IL-2 IL-17a
Rantes Eotaxin-3 IL-4 IL-1b

 

CNTF IGF-BP1 IL-21 Neuroserpin
CCL27 IL-3 IL-22 NOV
CTGF IL-5 IL-31 CXCL12
CXCL16 IL-7 CXCL11 TACI
EG-VEGF IL-9 MIA-2 TIMP-1
CXCL1 IL-11 Midkine TL-1A
CXCL2 IL-17E CXCL9 TSLP
ICAM-1 IL-20 CXCL7 TWEAK

 

*New

TPO IL-1RA IL-33 TGFb3
HMGB1 mHLA-DR

 

Mouse

TNFa IL-1a PDGF-BB Eotaxin (CCL11) IL-5
IGF-1 IL-1b b-NGF Exodus-2 (CCL21) IL-15
VEGF G-CSF IL-17a IL-3 IL-23
IL-6 GM-CSF IL-2 IL-13 TPO
FGFb MCP-1 IL-4 IL-21 IP-10
IFNr M1P-1a IL-10 IL-22 MCP-2
EGF SCF Resistin IP-10 (CXCL10) IL-33
Leptin Rantes IL-12 KC (CXCL1)

 

Rat

TNFa IL-1a
VEGF IL-1b
FGFb IL-5
IFNr IL-6
Leptin IL-15
MCP-1 IP-10
SCF Rantes
M1P-1a TGFb

 

 


Process

To get started, please contact us 

  • Please provide us with a list of cytokines of interest.
  • The project will be initiated by a scientist and completed as soon as we can.

 

Citations

Extracellular signal regulated kinase 5: A potential therapeutic target formalignant mesotheliomas. Shukla A, Miller JM, Cason C, Sayan M, Macpherson MB, Beuschel SL, Hillegass JM, Vacek PM, Pass H,Mossman BT. Clin Cancer Res., 2013 Apr;19(8):2071-83.

Ursodeoxycholic Acid Halts Pathological Neovascularization in a Mouse Model of Oxygen-Induced Retinopathy. Thounaojam MC, Jadeja RN, Rajpurohit S, Gutsaeva DR, Stansfield BK, Martin PM, Bartoli M. Journal of Clinical Medicine. 2020; 9(6):1921.

Cytokine ELISA Strips

Currently the cytokine ELISA strips for simultaneously monitoring 8 cytokines are available at Signosis. Signosis also provides custom ELISA strip for profiling particular cytokines you are interested in.

Signosis is offering  custom mix & match Cytokine ELISA strip. You can select any up to 8 cytokines in our inventory (human, mouse and rat), and we can make your strip in your convenience from our large inventory.

 

Human

TNFa VEGF PDGF-BB IL-10
IFNr EGF PIGF-1 FGFb
G-CSF IL-6 b-NGF Leptin
GM-CSF Resistin SCF IGF-1
IL-1a PAI-1 MCP-1 TGFb
IL-8 IL-12 MIP-1a Adipo
IP-10 IL-13 IL-2 IL-17a
Rantes Eotaxin-3 IL-4 IL-1b

 

CNTF IGF-BP1 IL-21 Neuroserpin
CCL27 IL-3 IL-22 NOV
CTGF IL-5 IL-31 CXCL12
CXCL16 IL-7 CXCL11 TACI
EG-VEGF IL-9 MIA-2 TIMP-1
CXCL1 IL-11 Midkine TL-1A
CXCL2 IL-17E CXCL9 TSLP
ICAM-1 IL-20 CXCL7 TWEAK

 

*New

TPO IL-1RA IL-33 TGFb3
HMGB1 mHLA-DR

 


Mouse

TNFa IL-1a PDGF-BB Eotaxin (CCL11) IL-5
IGF-1 IL-1b b-NGF Exodus-2 (CCL21) IL-15
VEGF G-CSF IL-17a IL-3 IL-23
IL-6 GM-CSF IL-2 IL-13 TPO
FGFb MCP-1 IL-4 IL-21 IP-10
IFNr M1P-1a IL-10 IL-22 MCP-2
EGF SCF Resistin IP-10 (CXCL10) IL-33
Leptin Rantes IL-12 KC (CXCL1)

 

Rat

TNFa IL-1a
VEGF IL-1b
FGFb IL-5
IFNr IL-6
Leptin IL-15
MCP-1 IP-10
SCF Rantes
M1P-1a TGFb

 

Process


To get started, please contact us

  • If you are interested in the custom Cytokine ELISA Strip, please let us know which cytokines you want to replace or provide us with a new list of the cytokines you are interested in.
  • The project will be initiated by a scientist and completed as soon as we can.

miRNA Arrays

Currently a series of miRNA arrays for monitoring multiple miRNAs are available at Signosis. Signosis also provides custom service for development of miRNA array for profiling particular miRNAs you are interested in.

Process

  • To get started, please contact us 
  • Please provide us with a list of the miRNAs you are interested in.
  • The project will be initiated by a scientist and completed as soon as we can.

miRNA Northern Blot Assay Kits

100+ miRNA northern blot assay kits are currently available at Signosis. Signosis also provides custom services to prepare any miRNA of interest.


Process

To get started, please contact us 

  • If you are interested in the custom miRNA northern blot assay kit, please let us know the sequence of the miRNA.
  • The project will be initiated by a scientist and completed as soon as we can.

miRNA Real-Time PCR

100+ miRNA Real-Time PCR kits are currently available at Signosis. Signosis also provides custom Real-Time PCR for any unlisted miRNA you are interested in.


Process

To get started, please contact us

  • If you are interested in the custom miRNA Real-Time PCR, please let us know the sequence of the miRNA.
  • The project will be initiated by a scientist and completed as soon as we can.

Co-IP Kits

Co-immunoprecipitation kits for several different proteins are available at Signosis. Signosis also provides custom service for development of Co-IP Kits for unlisted protein you are interested in.

Process

  • To get started, please contact us 
  • If you are interested in the custom Co-IP Kit, please let us know the detailed information of the protein.
  • The project will be initiated by a scientist and completed as soon as we can.

 

Signosis provides protein expression services for expression of recombinant proteins such as SH2 domains in E. Coli.


Process

  • To get started, please contact us 
  • If you are interested in the protein expression service, please let us know the detailed information about the type and the number of your samples, the name of the protein, and any additional information.
  • The project will be initiated by a scientist and completed as soon as we can.

Cytokines and Hormones

Cytokines and growth factors are signaling molecules that have critical roles in many biological processes such as cellular growth, differentiation, Read More…

Biomarkers & Key Proteins

The presense or altered level of a specific molecule or biomarker can help to confirm a particular disease state. The Read More…

miRNA Research

miRNAs are small non-coding RNA molecules that regulate up to 30% of mammalian gene expression. They serve as post-transcriptional regulators, Read More…

TF ELISA Kits

  Benefits Highly specific – Specifically detect activation by binding sequence and with  an antibody against the specific gene. High-throughput – With Whole Cell Read More…

Gene Expression Analysis

Gene Expression is the process by which information from a gene is used to direct the synthesis of a functional gene Read More…

miRNA Real Time PCR

Principle In the assay, the target miRNA molecule is hybridized with two oligos to form a RNA/DNA duplex. When the Read More…

Transcription Factor Assays

Transcription factors (TFs) are DNA binding proteins that play essential roles in regulating gene expression. They act as a sensor to Read More…

Resisting Cell Death

In order to survive and proliferate, cancer cells must overcome and avoid the diverse range and number of cell death Read More…

Oligos and Amplification

Overview PCR is a mainstay Molecular Biology technique and over the years has been refined, developed and advanced to become Read More…

Analysis of PCR

Overview Analysing and visualising your results is often the culmination of weeks if not months of work and can be Read More…

Genetic Engineering – Master the Essentials

Overview In Molecular Biology, the ability to successfully manipulate DNA is essential.  Stratech offers cutting edge cloning systems, genome editing Read More…

Visualisation

  Overview There are many ways of visualising your results; some of them more visual than others! Stratech supply sensitive Read More…