We are the tech transfer platform that brings innovation into life science workflows. We produce the most innovative R&D tools, side by side with the researchers who design, test and use them.
Did you ever feel thrilled, discovering a life science know-how in a publication that could positively change your research project? We did. And we remember the growing frustration… being unable to reproduce that know-how in our own labs. Or unable to find someone who could introduce us to the authors to ask them for a test.
At Idylle, we want to help researchers smoothly try the innovative tools they want. Even if they have just been published. Even if they seem out of reach.
Scientists can try the latest life science tools. They can get an eye on forthcoming innovations. And they can start new collaborations with the authors.
The life science tools we co-develop, produce and deliver are all designed by researchers. They are meant for communities of academic and private researchers. They are produced on demand. They are fully assembled in our laboratories and made in France. They are safely shipped around the world. Read more on our process of co-development.
We establish a collaboration framework together: they handle all the scientific operations. We handle all the rest (see how). In a nutshell, it means setting up a reviewing process, designing a stable product, producing on-demand to fit the needs of every user, controlling the quality, testing, writing the protocols, designing the packaging, handling the MTAs, the customs… Everything scientists don’t feel like managing on a large scale. Together, we widespread their know-how.
Contact us to disseminate your know-how . Or just discuss a tool co-development project.
Academic and private researchers end up swiftly testing their research ideas with innovative tools. They grow their scientific collaborations. They generate new ideas together.
Idylle was born in 2019 within the start-up studio Quattrocento. In November 2021, the CNRS entered the capital of Idylle.
This partnership gives more power to the new transfer solution invented by Idylle, 100% dedicated to innovations in research tools. All together, we want to support researchers who will circulate their innovations around the world more effectively, and thus generate new ideas and collaborations. A true circular knowledge economy is on its way!
What is it intended for? Use it to convert your inducible ERT model into a photo-inducible one. Use it if you want to control transcription (using Gal4-UAS) or induce recombination (using Cre-lox) in space and/or time for in-vivo cell tracking experiments and more.
Product information: Kit description, Safety datasheet and FAQ
Designed by Ludovic Jullien, Isabelle Aujard and Thomas Le Saux
Reviewed by David Bensimon, Lorenzo Bombardelli, Sidney Cambridge, Cristina Pujades, Angel Raya, Alexandre Specht, Perrine de Villemagne
Wide applicative scope
Technology capitalizing on the versatile use of Tamoxifen-OH for controlling functions of multiple types of proteins.
Caged Cyclofen-OH is cell-permeant and can be added either in the external medium or directly injected for conditioning.
Excellent chemical stability
Caged Cyclofen-OH does not generate any basal activation of protein function and it benefits from an excellent temporal resolution upon uncaging.
Favorable wavelength ranges for uncaging
Uncaging requires either UV-A light or a strong IR laser. Visible light is inactive, which facilitates the experiments with biological samples
Caged Cyclofen-OH liberates Cyclofen-OH, which is photostable in contrast to Tamoxifen-OH encountering photodegradation under illumination.
One 5mg vial
The volume of DMSO required to reach a concentration of 10mM is indicated on the vial
We can also prepare a 50mg vial. Email firstname.lastname@example.org for this special mice quote.
Actiflash Safety Datasheet
Actiflash Certificate of Analysis
” Once upon a time, a physicist (David Bensimon) asked a chemist (Ludovic Jullien) whether he could design a caged inducer to photocontrol protein activity in living organisms. For sure! However we also needed a biologist (Sophie Vriz) to accept the challenge to validate the caged Cyclofen-OH technology. It has been a long but so nice adventure, which has involved the tight integration of the work from many talented students, postdocs, and collaborators… Thanks to all of them!”
“Actiflash perfectly highlights the researchers’ need to calibrate the experimental conditions in life sciences. We wanted to initiate a global scheme on this topic and find solutions to help them get reproducible results. So, when we met Ludovic Jullien’s team, it was obvious to us that Actiflash was a first step in the right direction. Their technology had already been tested and approved by a large number of researchers. It was then a question of making it a stable product, of producing it and disseminating it to the research communities. It is a great satisfaction to propose it today and we are very proud that Ludovic Jullien’s team has chosen to widespread its technology with Idylle! Ludovic has extensive experience in transfer options, and his choice also reinforces our approach of promoting the know-how of researchers!”
What is it intended for? Use it if you want to image bacteria both still and alive under the microscope. Or if you want to perform long-term imaging of bacteria. Or if you want to change the growth condition (e.g. antibiotics, chemicals, inhibitors) during the experiment and directly observe, in real-time, the bacteria new comportment under the microscope.
For which bacteria? E. coli, Vibrio cholerae, Myxococcus xanthus, Mycobacterium smegmatis, Bacillus subtilis, Pseudomonas aeruginosa and Pseudomonas fluorescens. More bacteria being currently tested by our Test Program users.
Product information: Kit description, Lifetime, FAQ, Technical and Safety datasheets
Designed by Tâm Mignot, Olivier Theodoly, Amandine Desorme, Guillaume Sudre and Laurent David
Published in mBio
“Cells expressing HaloTagged DNA repair proteins were loaded onto the chitosan-coated coverslip and imaged with TIRF microscopy. The Chitozen technology enables tracking single-molecules while changing environmental conditions (e.g: M9 or M9 + mutagen). Computation of the diffusion coefficients provides insights into how proteins change their mobility upon DNA damage”.
Credit of all images and the video: Maxence Vincent – University of Oxford – 2022
Time-lapse images of E. coli growing: Localization of F-plasmid
By Jerome Rech, LMGM-CBI-CNRS, Toulouse, 2022
Cells are observed in phase contrast (top), in the blue and yellow channels for fluorescence microcopy to observe phase contrast, nucleoid (DAPI) or F-plasmid (mVenus tag), respectively.
Overlays of phase contrast and blue or yellow channel are shown on middle or bottom panels, respectively.
Cells were grown at 30°C in supplemented M9 minimal media containing Cystein and DAPI under continuous flow. Scale bar (2 µm).
Full compatibility with most of your conditions of experiments
Size: the coverslip dimension (25×75 mm) is compatible with the most common available sticky slides and microscope stages.
Compatible with advanced microfluidic techniques, nanolithography
6 independent channels
Either perform up to 6 experiments at the same time or use 1 channel one day, and the others later
Ready and fast
Assemble it within 2 minutes
Use it the same day it is prepared
A bench-stable surface coated with chitosan, the most efficient way to immobilize your bacteria on a microscope coverslip
Storable for 2 months once assembled
Chitozen: how to use it, a video protocol from assembling the elements to imaging your favorite bacteria.
µ-Slide Microscopy Rack
Eppendorf® Centrifuge 5430 & Rotor 5430R A-2-MTP
Clamp and adapter for sticky slides
Bottomless 6 channels sticky slides (sterilized and welded in a gas-permeable packaging)
LB ½ medium
5-Chitozen Standard kit
5 standard (25 x 75 mm) chitosan-coated coverslips with 5 sticky slides
1-Chitozen Starter kit
1 standard (25 x 75 mm) chitosan-coated coverslip with 1 sticky slide
Each Chitozen coverslip allows up to 6 assays using bottomless 6-channel sticky slides
Lifetime: up to 12-month storage at room temperature, shielded from direct sunlight.
Actiflash Safety Datasheet
Actiflash Certificate of Analysis
Chitozen coverslips allow bacterial cells to be immobilized even under a flow.
What is it intended for? Use it if you want to use a hassle-free buffer to decouple the preparation of your samples and their imaging with dSTORM. For instance if you need to prepare your samples in your lab, and image them in your core facility 3 to 4 weeks later. Use it if you want to image with red, far-red and green dyes (eg. AF488). Use different colors either at the same time or one after the other.
Product information: kit description, custom Everspark, lifetime, FAQ, Safety datasheet and Certificate of analysis
Designed by Karine Monier, Arnaud Favier and Christophe Place
Reviewed by Pierre Bon, Ingrid Chamma, Benjamin Compans, Laurence Dubreil, Beatrice Durel, Tamas Gajdos, Sébastien Mailfert, Céline Malleval, Paolo Marchi, Delphine Muriaux, Jonny Nixon-Abell
Powered by the Eternity technology published in Scientific Reports
Everspark works with thick samples of 100µ (peripheral blinking).
Testers are currently trying those conditions:
Thick samples of 100µ (peripheral blinking)
Multiconditions at the same time or sequentially: High Content Screening / High Troughput
We would love to know if somebody is testing PALM + STORM at the same time.
The community also disclosed that Everspark does not work 100% efficiently with organs and tissues because there is most of the times a bit of remaining oxygen.
Fluorescent Polymer-AS1411-Aptamer Probe for dSTORM Super-Resolution Imaging of Endogenous Nucleolin
Fabre L, Rousset C, Monier K, Da Cruz-Boisson F, Bouvet P, Charreyre MT, Delair T, Fleury E, Favier A. Biomacromolecules. 2022 May 12. doi: 10.1021/acs.biomac.1c01706. PMID: 35549176
Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.
Michalik S, Siegerist F, Palankar R, Franzke K, Schindler M, Reder A, Seifert U, Cammann C, Wesche J, Steil L, Hentschker C, Gesell-Salazar M, Reisinger E, Beer M, Endlich N, Greinacher A, Völker U. Haematologica. 2022 Apr 1;107(4):947-957. doi: 10.3324/haematol.2021.280154. PMID: 35045692
Insights in ChAdOx1 nCoV-19 vaccine-induced immune thrombotic thrombocytopenia.
Greinacher A, Selleng K, Palankar R, Wesche J, Handtke S, Wolff M, Aurich K, Lalk M, Methling K, Völker U, Hentschker C, Michalik S, Steil L, Reder A, Schönborn L, Beer M, Franzke K, Büttner A, Fehse B, Stavrou EX, Rangaswamy C, Mailer RK, Englert H, Frye M, Thiele T, Kochanek S, Krutzke L, Siegerist F, Endlich N, Warkentin TE, Renné T.
Blood. 2021 Dec 2;138(22):2256-2268. doi: 10.1182/blood.2021013231. PMID: 34587242
Superresolution Microscopy of Drosophila Indirect Flight Muscle Sarcomeres.
Szikora S, Novák T, Gajdos T, Erdélyi M, Mihály J. Bio Protoc. 2020 Jun 20;10(12):e3654. doi: 10.21769/BioProtoc.3654. eCollection 2020 Jun 20. PMID: 33659324
High stability over time for repeated measurements
One mounted sample is stable over 3 to 4 weeks and can be imaged multiple times
Multicolor (incl. green)
Compatible with green, red & far red dyes (JF646, JF549, AF647, CF647, DL550, CF568, DL650, CF680, SulfoCy5) and mEos2
Ready to use
Can be used directly from the vial
Up to 6 months performance
Individualized packaging for optimal longevity
HILO, TIRF pattern
Choose the MEA concentration, pH, optical index, or even additives
Note 1: Blinking events have been validated with JF646, JF549, AF647, CF647, DL550, CF568, DL650, CF680, SulfoCy5 and mEos2
Note 2: Blinking is detected for several weeks without loss of events or photons per event
Note 3: Mounting can also be performed in various microdishes by adjusting the Everspark volume (Each vial contains 450 µl)
Up to 6 months (2 months after opening the pouch + 4 months storage in the pouch).
10 vials with 450 µL or 150 µL of Everspark buffer at 100 mM MEA in Tris
1 vial per experiment
YOUR CUSTOM EVERSPARK
10 vials or your own Everspark
Choose the MEA concentration, volume and pH. See below.
Tune the MEA concentration, the volume or the pH
Ask for a Custom Everspark quote, and our product development team will come back to you.
You want something even more specific?
Contact us and we’ll get back to you with a quote. We’ll do everything to deliver the product that fits your experimental needs!
“When I was working on long manipulations, I dreamed of being able to stop at certain crucial stages. Everspark is the ideal answer to the problem of many researchers who prepare their samples in a hurry simply because they need to image them right away. The Everspark buffer eliminates this tension: they take the time to image whenever they want, with complete peace of mind. They can also make acquisitions over several days. This will really change the way people prepare their experimentations, and that’s exactly what I like about my job!
In terms of production, I am very proud of the way we have managed to set up a just-in-time production process in our labs in record time. And when researchers ask us to introduce variants, our production teams are able to build and produce very quickly their customized buffer to test it”.
A technology originally designed by Gaëlle Recher for spheroids & organoids. Powered by the Mount(n) technology (UniverSlide) published in Scientific Reports. Optimized by Idylle thanks to the Test Program feedback for zebrafish and medaka
Those little extras that make all the difference:
See the technical information about each shape in details.
I successfully managed to use Stampwell with Collagen-1.
Matthieu Opitz • Alveole, France
I use the Stampwell V-shape to track the explants development and the embryoids formation from stem cell aggregates in zebrafish, and it makes my work much easier!
Queralt Tolosa • Centre for Genomic Regulation, Spain
I used a Stampwell to ensure that my zebrafish embryos were positioned correctly under the microscope for spatial and temporal experiments. I am pleased with my Stampwell because it saves me time and provides precision!
Shai Eyal • UCSD, USA
Stampwell has proven to be a very easy to use mounting medium for confocal imaging of fixed and immunostained zebrafish embryos.
Matthieu Simion • TEFOR, France
Live imaging of beating hearts in early zebrafish embryos using the Larvae 1 Stampwell
Anesthetized 3 dpf embryos expressing a fluorescent biosensor in the heart were mounted in agarose microwells made using the Larvae 1 Stampwell and imaged in ventral view.
Credits: Prof. Juan Llopis – CRIB, Universidad de Vastilla La Mancha, Spain
Utilizing the Stampwell V-shape when turning airway organoids inside out is useful to keep them separated, therefore avoiding clumping and merging.
Signe Lolle • Danmarks Tekniske Universitet, Denmark
Apical-out 10-day human airway organoids imaged in a V-shape Stampwell in comparison with suspension plates
Airway organoids were generated from a human bronchial cell line in hydrogel and transferred either to microwells made using a V-shape Stampwell, or suspension plates, for turning them inside out and imaging.
Credits: Signe Lolle – Danmarks Tekniske Universitet, Denmark
Imprinted V-shape 300µ well in agarose
Soaked in fluorescein, negative rendering
Image credit: (c)Gaëlle Recher – Bordeaux
Cell-filled alginate capsule in the V-shape well, imaged in z
Notice that the focus moving through the well height is easily visible (lines in focus Vs out of focus)
Image credit: (c)Gaëlle Recher – Bordeaux
Improved positioning of 2dpf zebrafish larvae using the Embryo 2 Stampwell
Credits: Dr Rui Monteiro – Institute of Cancer and Genomic Sciences, University of Birmingham, United Kingdom
Heart imaging in a Larvae 1 Stampwell
Top picture: green-heart 3dpf zebrafish larvae placed in ventral view. Middle & bottom pictures: 3dpf zebrafish larvae PFA-fixed, mounted in glycerol and inserted in Larvae 1 Stampwell (anterior to the left). Pictures were taken using a Leica Spectral Confocal SP5.
Credits: Dr. Giovanni Risato, Prof. Natascia Tiso and Alina Ramazanova – Department of Biology, University of Padova, Italy
Compatible with most 35 mm dishes and 6-well plates
Use your stamp dozens of times
Safe for transport
Keep samples at the bottom of the wells, even when a dish is upside dow
Long-term imaging over weeks
Samples lay on the bottom of the well. They can freely grow within it to image multiple times.
create an organized network of samples to identify the samples for a posteriori experiments
* for > 10 wells Stampwells
Pour liquid agarose
Place the stamp
Reticulate the hydrogel
Remove the stamp
To image, just load your samples and image, sequence, transport at will.
To aggregate, seed your cells & let them aggregate.
V Shape 300µ:
. Number of pins: 42
. Depth of the well: 1 mm
. Well bottom diameter: 300µm – Shape: circular
. Well upper side: 1mm*0.5mm – Shape: rectangular
V Shape 500µ:
. Number of pins: 42
. Shape of the pins: V
. Depth of the well: 1 mm
. Well bottom diameter: 500µm – Shape: circular
. Well upper side: 1mm*0.5mm – Shape: rectangular
Embryo 1 (aka Rectangular):
. Number of pins: 35
. Shape of the pins: rectangular
. Length * width of the wells: 2 mm * 0,65 mm
. Number of pins: 18
. Shape of the pins: a drop
. Length of the wells: 3.90 mm
. Largest width of the wells: 0.88 mm
. number of pins: 5 large pins ideal for the 9-14 dpf zebrafishes + 5 medium pins ideal for the 6-9 dpf zebrafishes + 5 small pins ideal for the 3-6 dpf zebrafishes
. Shape of the pins: fish body and tail
. Length of the 5 large wells: 6 mm, 2.7 for the body and 3.3 for the tail
. Length of the 5 medium wells: 5 mm, 2.25 for the body and 2.75 for the tail
. Length of the 5 small wells: 4 mm, 1.8 for the body and 2.2 for the tail
. Number of pins: 10. 5 pins for zebrafish larvae and 5 pins for the medaka larvae on the same stamp
. Shape of the pins: a prism.
. Length of the wells: 10 mm for the 5 long wells and 3 mm for the 5 small wells.
. Depth: 1 mm
. Width at the bottom of the wells: 0.3 mm
. Width of the upper side of the wells: 2 mm
What is it intended for? Use it notably for wound-healing assay & immunocytochemistry experiments. It is very helpful when you are working with super expensive reagents or very rare cell lines, and when you want to test various experimental hypothesis.
No limit! Choose a ready-to-use design (Solo – Quartet – Nonet – Presto – Allegro). Or design your own shapes & stack them freely.
The “Starter kit” provides 2 sheets of 50 Stencells each. Kit descriptions, designs, Technical datasheet and FAQ
Designed by Vincent Studer, Pierre-Olivier Strale and Aurélien Pasturel
They were hosted by the Cell Organ-izers joint research laboratory (CNRS-Alvéole).
Powered by the Stencil technology published in Advanced Healthcare Materials .
COS-7 cells colonizing a gap induced by the removal of a PDMS stencil.
Images shot with a 20X objective during 18 hours (1 image every 2 min) with an inverted microscope.
Image credit: © Pierre-Olivier Strale
We produce it with the highest standards of quality, whether you want 1 or 100 copies
Compose flow channels and chambers, stacking different designs of Stencell
So far, it has been successfully stuck on:
Stencell is not glued. It can then be removed to:
Trigger cell migration
Switch from flow chamber to open window
Very useful for wound healing experiments
These thin sheets of silicone are fully transparent, without autofluorescence
One multiwell Stencell can perform several experimental conditions
A few microliters only are required
Key conditions of success
Protocol of use
The “Starter kit”
– 2 sheets of 50 Stencells each
– Either the same design for the 100 Stencells, or 2 different designs (one design per sheet).
The “Get your own design” kit:
– 1 design study to develop your project
– 2 sheets of 50 Stencells each (priced as the ones available in the “Starter kit”)
– You can mix 1 sheet of your design with 1 sheet of the already existing ones.
Each sheet is composed of 50 identical Stencells.
Upon receipt of your purchase order, you will be able to choose either the same design for your 2 sheets, or 2 different designs for your 2 sheets (1 per sheet of 50).
– Solo: 1 circular well – Diam. 12 mm
– Quartet: 4 circular wells – Diam. 3 mm
– Nonet: 9 circular wells – Diam. 3 mm
– Presto: 2 oblong wells spaced by 0.35 mm
– Allegro: 2 oblong wells spaced by 0.62 mm
The Stampwell most Frequently Asked Questions
“While developing an innovative micropatterning technology on glass coverslips, we had to optimize our protocole and thus screen a lot of conditions with expensive reagents. We could have used glass-bottom 96wp but we found them expensive and inconvenient for reproducible surface treatment. We thus started to develop our own low-volume fluid handling method. We quickly discovered that hydrophobic perforated PDMS films could act as reversible boundaries for liquids. With simple craft cutting robot, we were able to fabricate on-demand stencils of any shape and size. By filling those stencils with microliters droplets, we end up with a very versatile and reproducible high-throughput fluid handling solution.”
“Sometimes research needs a high level of versatility. This is exactly what appealed me when I met Vincent, Pierre-Olivier and Aurelien. They were hosted by the Cell Organ-izers joint research laboratory, set up by CNRS and our sister company Alveole. So they naturally turned to us when they decided to look for a partner to release their stencils. We knew that Stencell would be so useful to a lot of researchers who could save reagents and cells while testing new ideas and experimental conditions on small volumes. So we said yes immediately. On top of that, the three of them have proven to be highly reactive and proactive, engaged and enthusiastic. They love as much as we do testing new ideas and suggest others. It was a great pleasure to go through our industrial process together!”
SpheroRuler is great for dSTORM calibration experiments because you can use it on your preferred support and buffer. And because you get confident with the new biological structure to image. SpheroRuler also produces simple shapes that are easy to study when you are a dSTORM beginner!
How do you know your imaging process is good with SpheroRuler? Your beads blink, you reconstruct a ring in 2D and a sphere in 3D experiments, and you check that you retrieve the 1 µm diameter.
A technology designed by Arnaud Favier, Karine Monier and Christophe Place.
Suited for use in SMLM microscopy
Thoroughly characterized by electron microscopy
with sharp and thin edges
Suitable for drift correction applications
Resuspended in an aqueous buffer and usable along biological samples such as cells or tissue sections
A high fluorescence intensity for practical use as demo or training tools
SpheroRuler beads imaged using SRRF-Stream super-resolution microscopy
(A, B). Pictures of SpheroRuler beads acquired in wide-field (A) and SRRF-Stream super-resolution (B) imaging. Scale bar = 5µm (C, D) Local enlargements of A and B pictures respectively.
Scale bar = 1µm (E) Fluorescence intensity distributions along the solid lines in C and D.
Credits: Yao Baoli – Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, 2023
2D and 3D dSTORM images of a SpheroRuler bead acquired with a Leica GSD system
Credits: Lydia Y Li, Nabi Lab, The University of British Columbia, 2023
1 vial of 50 µL of a SpheroRuler suspension in PBS pH7.4.
Concentration: 7.10exp8 particles per mL.
Allows 10 experiments when using the recommended 5µL volume in 35mm glass-bottom dishes.
Stability: up to 7 months when stored at 4°C.
Dye: 647-fluorophore (far-red fluorescence).
Compatible with: dSTORM, SRRF, Airyscan confocal, confocal, SEM
Q/ What are SpheroRuler beads made of?
The SpheroRuler beads are 1μm-diameter polymer particles surrounded by 647-fluorophores
covalently anchored to their surface. Beads will be visible as hollow rings or spheres when
reconstructed in 2D or 3D SMLM experiments respectively.
Q/ Which types of imaging is SpheroRuler compatible with?
SpheroRuler beads are coated with 647-fluorophores giving a stable blinking in SMLM
micrsocopy, and have been initially developed for dSTORM imaging. Since then, they have also
been successfully used in SFFR, SEM, confocal, Airyscan confocal and SEM microscopy.
Q/ How accurate is the SpheroRuler bead size?
The spherical particles making up Spheroruler beads have been selected based on very good
monodispersity properties. The accuracy and reproducibility of the bead diameter have been
characterized by SEM on 25 independent microspheres and showed a standard deviation of
1 +/- 0.05μm.
Q/ What is included in the SpheroRuler kit? Is there anything I need that is
The SpheroRuler kit contains a 50μL suspension of SpheroRuler beads. All you need to have
on your side is some blinking buffer, coverslips and imaging vessels of your choice.
Q/ How many experiments can I carry out with one SpheroRuler kit?
One kit contains a 50μL suspension of SpheroRuler beads, allowing for 10 experiments when
using the recommended 5μL volume in 35mm glass-bottom dishes.
Q/ Can I use it alongside my biological samples?
Yes, SpheroRuler beads are resuspended in PBS and can be loaded together with biological
specimens (cells, tissue sections, etc).
Q/ For how long can I keep my SpheroRuler solution?
The SpheroRuler suspension is stable for at least 7 months when stored at 4°C.
Q/ Is there any specific reconstruction algorithm I should use?
SpheroRuler beads are highly fluorescent beads coated with a high density of fluorophores.
We recommend using a multi-emitter fitting approach, or any equivalent, to guarantee an
efficient localization of individual blinking events. Although the available options will vary on
each imaging system used, examples of algorithms that were successfully used include the
“account for overlap” function on the ZEN software, or the “high-density”mode on the Zeiss
software and the freely available UNLOC software.
Q/ How should fluorophore thickness be taken into account in the diameter
Fluorophores are directly coated on the bead surface without linkers or antibodies, and their
thickness is therefore negligible compared to the measured diameter. The apparent thickness
of the fluorophore ring will depend on the resolution of your imaging system (i.e. around 160-
200nm when measured in dSTORM). The external periphery of the beads should be taken into
account when measuring diameter.
Q/ I am not retrieving a perfectly spherical shape when reconstructing the
beads in 3D. Is it normal?
Although 2D reconstructions of SpheroRuler beads should be accurately circular, obtaining an
elongated shape in z is a common artefact that will depend on the system used for imaging
and for 3D reconstruction (biplane reconstruction, astigmatism, etc). The measured diameter
in z and its distance from the actual 1μm can therefore be used as a robust indicator to
evaluate the fidelity of the 3D reconstruction for a given system. As an example, measured z
diameter was 1.3 μm when tested on a Vutara VXL system using biplane imaging.
“It could have been one of those after-dinner conversations that would have ended there. Arnaud, Karine, Christophe and Mathieu, from Idylle, had a lively discussion about some of our experimental conditions in dSTORM. They came to talk about the confidence we can place in our instruments. Mathieu launched: “You should develop a calibration tool for dSTORM, it would be of great help for so many researchers”. This little phrase hung in the air… until we decided that it was a challenge that we liked and that we were going to take up. Today we are happy to launch SpheroRuler with the whole Idylle team.”
“Calibration beads for dSTORM? Why hadn’t we thought of that before? Their very simplicity was praised by early researchers who tested and adopted them. Scientists new to SMLM greatly appreciate their ease of use. We are all very happy here that this idea of a night has become a research tool for all “.
It is very helpful if you want to analyze how your cells will react if you squeeze them for a prolonged period. Or if you want to study how mechanical confinement affects drug cell resistance. And if you want to perform immunostaining in situ.
Designed by Audrey Prunet, Gilles Simon, Hélène Delanoë-Ayari, Véronique Maguer-Satta and Charlotte Rivière who tell the story of their transfer here .
Published in Lab on a chip
Human: primary T-lymphocytes, TF1 & ML2 leukemic cells, HS27A fibroblasts, MCF10A breast cells, MDA-MB-231 breast cancer cells, U-2 OS osteosarcoma cells, PC-3 & DU 145 prostate cancer cells, HT29 & HCT116 colorectal adenocarcinoma cells, HT1080 fibrosarcoma cells and megakaryocytes
Murine: osteocyte-like cells MLO-Y4, primary muscle cells & primary dendritic cells
Plant: cells from Arabidopsis roots
3D cell objects: mice gastruloïds
Compression of mouse primary myoblasts using AgarSqueezer
C57 primary myoblasts stained with Hoechst were imaged in the AgarSqueezer before compression (left panel), and after 1.5h of compression under 2.5 µm height pillars (middle & right panels).
Credits: Dr. Hind Zahr & Dr. Alice Varlet, Lammerding Lab – Meinig School of Biomedical Engineering, Cornell University, United States
Arabidopsis root cells confined using Agarsqueezer
Arabidopsis thaliana Col-0 root cells stained with Calcofluor (cell wall) and imaged with a confocal microscope either in a traditional culture setting (left), or after 24h of confinement in the Agarsqueezer using the 30µm (middle) or 5µm (right) pillars.
Credits: Léa Bogdziewiez – UPSC – Sveriges lantbruksuniversitet, Sweden
Tunable stiffness in a physiological range [1-150] kPa
Use of agarose as a cheap and biocompatible polymer
Open access to the reservoir
Possibility to add drugs, and reagents
Easy medium renewal
Autoclavable & reusable systems
Compatibility with multiple microscopy techniques
Confocal, spinning, super-resolution
Open access for microscope objectives
Use of optical glass coverslip to make cells grow
Easy to recover coverslip with cells for subsequent molecular analysis
FACS, qPCR, Western-Blot, Immunofluorescence (possible in situ)
Long-term analysis of the cell adaptation to confinement
Up to several days, for time-lapse studies
Study of the specific impact of mechanical loads on the biology of cells
Gas permeability of the system allows to get rid of the hypoxia conditions
Easy to assemble and disassemble the system
AgarSqueezer – How to use it – A piece of protocol in 3 steps
Check the most Frequently Asked Questions (FAQ).
You can choose among 2 kits:
– The “1 AgarSqueezer kit” contains 1 AgarSqueezer device
– The “2 AgarSqueezers kit” contains 2 AgarSqueezers devices.
In addition to the device, each kit contains also:
– 1x insert to hold up to 2 AgarSqueezers on the microscope stage
– 1x 16G flat cut needle to make holes in the agar gel, facilitating diffusion of culture medium or drugs during the experiment
– 1x 20G flat cut needle (same)
For users who also need a wafer to mold agarose, we can provide it in 4 different heights:
. 2.5 µm to highly confine cells
. 5 µm to moderately confine cells
. 30 µm not to confine cells (negative control)
. 100 µm for 3D confinement
“In the beginning, in Charlotte’s team, we wanted to analyze the influence of both stiffness and confinement on cells, mimicking highly confined situations such as fibrosis or cancer.
On its side, Veronique’s team was looking for a way to analyze the effect of long-term confinement with the ability to add a drug at any time.
We all wanted to find a device that would also meet the requirements of subsequent classical molecular analysis (easy cell culturing and cells recovery, qPCR, western-blot, in situ immunostaining) as well as biophysical image-based analysis (high-resolution microscopy and video-microscopy). But we could not find any.
So all together, we decided to collaborate on the perfect set-up. We rapidly identified agarose as an interesting material to get medium and oxygen renewal, with no drug adsorption. We built several prototypes, improving precise control of the confinement, avoiding destruction of the gels and leakage of the culture medium! And then we came up with the Softconfiner device, that we published and decided to transfer.
Today, we believe that the AgarSqueezer could be of interest for many researchers willing to better understand how mechanics can regulate cell behavior. We hope that researchers from different communities will also find it a useful tool!”
Audrey Prunet, Gilles Simon, Hélène Delanoë-Ayari, Véronique Maguer-Satta and Charlotte Rivière
“When Charlotte showed me for the first time their Softconfiner device, I got highly impressed by its level of maturity. The team of developers had managed to confine cells up to 8 days without affecting the cells viability, but had observed that mechanical confinement affected the cell proliferation of all the tested cell lines. Charlotte and the team were very clear on their motivation to make their Softconfiner easily accessible to the research community. In addition to being a pleasure to work together, it was quite obvious the technology would become a great product.” Esther Graudens
New projects at Idylle