We are the tech transfer platform that brings innovation into life science workflows. We produce the most innovative R&D tools, side by side with the researchers who design, test and use them.


Discover our portfolio of innovative R&D tools 

We co-develop the tools of the just published scientific know-how


Did you ever feel thrilled, discovering a life science know-how in a publication that could positively change your research project? We did. And we remember the growing frustration… being unable to reproduce that know-how in our own labs. Or unable to find someone who could introduce us to the authors to ask them for a test.

At Idylle, we want to help researchers smoothly try the innovative tools they want. Even if they have just been published. Even if they seem out of reach.


Scientists can try the latest life science tools. They can get an eye on forthcoming innovations. And they can start new collaborations with the authors.
The life science tools we co-develop, produce and deliver are all designed by researchers. They are meant for communities of academic and private researchers. They are produced on demand. They are fully assembled in our laboratories and made in France. They are safely shipped around the world. Read more on our process of co-development.


We establish a collaboration framework together: they handle all the scientific operations. We handle all the rest (see how). In a nutshell, it means setting up a reviewing process, designing a stable product, producing on-demand to fit the needs of every user, controlling the quality, testing, writing the protocols, designing the packaging, handling the MTAs, the customs… Everything scientists don’t feel like managing on a large scale. Together, we widespread their know-how.
Contact us to disseminate your know-how . Or just discuss a tool co-development project.


Academic and private researchers end up swiftly testing their research ideas with innovative tools. They grow their scientific collaborations. They generate new ideas together.


Idylle was born in 2019 within the start-up studio Quattrocento. In November 2021, the CNRS entered the capital of Idylle.
This partnership gives more power to the new transfer solution invented by Idylle, 100% dedicated to innovations in research tools. All together, we want to support researchers who will circulate their innovations around the world more effectively, and thus generate new ideas and collaborations. A true circular knowledge economy is on its way!


The stable Tamoxifen-like photoactivable inducer to perform a spatial and temporal control of your favorite proteins under illumination.

What is it intended for? Use it to convert your inducible ERT model into a photo-inducible one. Use it if you want to control transcription (using Gal4-UAS) or induce recombination (using Cre-lox) in space and/or time for in-vivo cell tracking experiments and more.

Product information:  Kit descriptionSafety datasheet and FAQ


Designed by Ludovic Jullien, Isabelle Aujard and Thomas Le Saux
Reviewed by David Bensimon, Lorenzo Bombardelli, Sidney Cambridge, Cristina Pujades, Angel Raya, Alexandre Specht, Perrine de Villemagne

Published in ChemBioChem



Wide applicative scope

Technology capitalizing on the versatile use of Tamoxifen-OH for controlling functions of multiple types of proteins.


Simple conditioning

Caged Cyclofen-OH is cell-permeant and can be added either in the external medium or directly injected for conditioning.


Excellent chemical stability

Caged Cyclofen-OH does not generate any basal activation of protein function and it benefits from an excellent temporal resolution upon uncaging.


Favorable wavelength ranges for uncaging

Uncaging requires either UV-A light or a strong IR laser. Visible light is inactive, which facilitates the experiments with biological samples


Photochemical stability

Caged Cyclofen-OH liberates Cyclofen-OH, which is photostable in contrast to Tamoxifen-OH encountering photodegradation under illumination.

Community Feedback We Found Interesting

Actiflash is efficient with zebrafish embryos of less than 48 hpf. It does not work over 48 hpf.


Simplified protocol & conditions of success


5 key conditions of success:

Method of use:


Calibration of the Actiflash concentration
It is advised to first establish the extent of phenotype sought for as a function of the Tamoxifen-OH concentration. Then the concentration of Actiflash used for sample conditioning is fixed at Tamoxifen-OH concentration causing 100% of the desired phenotype (in general 3-5 μM in cultured cells and zebrafish embryos).
Conditioning protocol with Actiflash
Incubate your samples in a serum-free medium for 90 mins, away from light.
Actiflash photoactivation
Illumination of Actiflash may be performed with UV (325-425nm range) light or multiphoton excitation (at 750 and 1064 nm with two- and three-photon excitation, respectively) to release Cyclofen-OH. You can use either benchtop UV lamps or light sources installed on
The calibration of the photoactivation
The objective is to provide enough photons to exhaust the conversion of the Actiflash but without generating detrimental side-effects on the biological sample.
Simply analyze the phenotype recovery with decreasing illumination duration. Then determine the shortest illumination duration leading to 100% uncaging of Actiflash.

Product Information

Kit Description

One 5mg vial
The volume of DMSO required to reach a concentration of 10mM is indicated on the vial

Want more?
We can also prepare a 50mg vial. Email sales@stratech.co.uk for this special mice quote.

Safety Datasheet & Certificate of Analysis

Actiflash Safety Datasheet

Actiflash Certificate of Analysis

Actiflash and us

” Once upon a time, a physicist (David Bensimon) asked a chemist (Ludovic Jullien) whether he could design a caged inducer to photocontrol protein activity in living organisms. For sure! However we also needed a biologist (Sophie Vriz) to accept the challenge to validate the caged Cyclofen-OH technology. It has been a long but so nice adventure, which has involved the tight integration of the work from many talented students, postdocs, and collaborators… Thanks to all of them!”

Esther Graudens, New projects, Idylle:

“Actiflash perfectly highlights the researchers’ need to calibrate the experimental conditions in life sciences. We wanted to initiate a global scheme on this topic and find solutions to help them get reproducible results. So, when we met Ludovic Jullien’s team, it was obvious to us that Actiflash was a first step in the right direction. Their technology had already been tested and approved by a large number of researchers. It was then a question of making it a stable product, of producing it and disseminating it to the research communities. It is a great satisfaction to propose it today and we are very proud that Ludovic Jullien’s team has chosen to widespread its technology with Idylle! Ludovic has extensive experience in transfer options, and his choice also reinforces our approach of promoting the know-how of researchers!”


The 1st functionalized microscope coverslip to image live bacterial cells & study their growth and behaviour.

Chitozen is a chitosan-coated coverslip compatible with 6-channel sticky slides.

 What is it intended for?   Use it if you want to image bacteria both still and alive under the microscope. Or if you want to perform long-term imaging of bacteria. Or if you want to change the growth condition (e.g. antibiotics, chemicals, inhibitors) during the experiment and directly observe, in real-time, the bacteria new comportment under the microscope.

 For which bacteria?  E. coli, Vibrio cholerae, Myxococcus xanthus, Mycobacterium smegmatis, Bacillus subtilis, Pseudomonas aeruginosa and Pseudomonas fluorescens. More bacteria being currently tested by our Test Program users.


Product information: Kit description, Lifetime, FAQ, Technical and Safety datasheets

Designed by  Tâm MignotOlivier TheodolyAmandine DesormeGuillaume Sudre and Laurent David
Published in  mBio

How to use Chitozen

E.coli & Chitozen

E. coli monolayers on Chitosan
© 2019 Tréguier et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

Visualization of Pal mCherry at septum in E. coli (W3110 Pal mCherry), by 3D SIM microscopy, in M9 medium and using Chitozen.
Credit : Amandine Desorme – LCB – CNRS – 2021

Feedback from the community

Chitozen has been successfully tested by our Test Program users on the following bacteria:
  •  E. coli
  • Mycobacterium smegmatis
  • Bacillus subtilis
  •  Pseudomonas fluorescens.
NB: These results supplement those obtained by Tâm Mignot and al. with:
  • E. coli
  •  Vibrio cholerae
  • Myxococcus xanthus
  • Pseudomonas aeruginosa.
Tracking of DNA repair proteins in live cells of Escherichia coli
By Maxence Vincent, University of Oxford, Jan 2022

“Cells expressing HaloTagged DNA repair proteins were loaded onto the chitosan-coated coverslip and imaged with TIRF microscopy. The Chitozen technology enables tracking single-molecules while changing environmental conditions (e.g: M9 or M9 + mutagen). Computation of the diffusion coefficients provides insights into how proteins change their mobility upon DNA damage”.

Credit of all images and the video: Maxence Vincent – University of Oxford – 2022

Time-lapse images of E. coli growing: Localization of F-plasmid
By Jerome Rech, LMGM-CBI-CNRS, Toulouse, 2022

Cells are observed in phase contrast (top), in the blue and yellow channels for fluorescence microcopy to observe phase contrast, nucleoid (DAPI) or F-plasmid (mVenus tag), respectively.

Overlays of phase contrast and blue or yellow channel are shown on middle or bottom panels, respectively.

Cells were grown at 30°C in supplemented M9 minimal media containing Cystein and DAPI under continuous flow. Scale bar (2 µm).



Full compatibility with most of your conditions of experiments

   Size: the coverslip dimension (25×75 mm) is compatible with the most common available sticky slides and microscope stages.

 Compatible with advanced microfluidic techniques, nanolithography


6 independent channels

Either perform up to 6 experiments at the same time or use 1 channel one day, and the others later


Ready and fast


 Assemble it within 2 minutes
Use it the same day it is prepared


Long lasting

A bench-stable surface coated with chitosan, the most efficient way to immobilize your bacteria on a microscope coverslip

   Storable for 2 months once assembled


The Chitozen video protocol

Chitozen: how to use it, a video protocol from assembling the elements to imaging your favorite bacteria.

Simplified protocol

The material you will need:

µ-Slide Microscopy Rack
Eppendorf® Centrifuge 5430 & Rotor 5430R A-2-MTP
Clamp and adapter for sticky slides


Bottomless 6 channels sticky slides (sterilized and welded in a gas-permeable packaging)


LB medium
LB ½ medium
Milli-Q® water


  1. coli – Mycobacterium smegmatis – Bacillus subtilis – Pseudomonas fluorescens – Vibrio cholerae –  Myxococcus xanthus – Pseudomonas aeruginosa.

Product Information

Kit Description

5-Chitozen Standard kit
5 standard (25 x 75 mm) chitosan-coated coverslips with 5 sticky slides

1-Chitozen Starter kit
1 standard (25 x 75 mm) chitosan-coated coverslip with 1 sticky slide




Each Chitozen coverslip allows up to 6 assays using bottomless 6-channel sticky slides

Lifetime: up to 12-month storage at room temperature, shielded from direct sunlight.

Safety Datasheet & Certificate of Analysis

Actiflash Safety Datasheet

Actiflash Certificate of Analysis

Going further: perfuse the system with flow

Chitozen coverslips allow bacterial cells to be immobilized even under a flow.

Chitozen and us

 We wanted to understand how our bacteria, Myxococcus xanthus, moves on a surface. So we tried to design a smart microscopy system to observe it. But there was a problem: the Myxococcus cells were not adhering to our glass slides. When we read a paper on how people use chitosan to get their bacteria better move on plates, we got an idea: maybe we could coat our glass slides with some chitosan? We tested it. And it worked! We then implemented our prototype with a microfluidics system to test the real time response of our bacteria to antibiotics. Now we’re thrilled to share it with the community of research! We hope that people working on bacteria will appreciate to use Chitozen because it is a very easy system to run that combines microfluidics and high-end microscopy. We are looking forward to getting feedback of use: it will mean that we have developed a device useful to the community and that is always a deep satisfaction!
Tâm Mignot, Olivier Théodoly, Amandine Desorme, Guillaume Sudre, Laurent David

Esther Graudens, New projects at Idylle

” We really loved the way all the team, whether in Marseille or in Lyon, was deeply concerned by the tiniest detail and willing to prepare the best ever product for the community. So if you want to know more about how we co-designed and produced Chitozen starting with chitosan, then catch an eye on   THE STORY OF CHITOZEN .
And if you are interested in seeing what you can do with chitosan-coated coverslips, you can have a look at this study published by the team of developers in 2019. They’ve managed to use chitosan-coated coverslips to promote the growth of cells without any deleterious effect on their physiology, allowing them to measure the antibiotic susceptibiliy of a diversity of clinical strains with an excellent accuracy in a very short period of time. Read all about it here: mBio , 2019″


The super-resolution microscopy buffer that offers multicolor, long-term and stable fluorescence imaging:
prepare your samples one day, and image them every day for weeks

What is it intended for? Use it if you want to use a hassle-free buffer to decouple the preparation of your samples and their imaging with dSTORM. For instance if you need to prepare your samples in your lab, and image them in your core facility 3 to 4 weeks later. Use it if you want to image with red, far-red and green dyes (eg. AF488). Use different colors either at the same time or one after the other.

Product information: kit description, custom Everspark, lifetime, FAQ, Safety datasheet and Certificate of analysis

Designed by Karine Monier, Arnaud Favier and Christophe Place

Reviewed by Pierre Bon, Ingrid Chamma, Benjamin Compans, Laurence Dubreil, Beatrice Durel, Tamas Gajdos, Sébastien Mailfert, Céline Malleval, Paolo Marchi, Delphine Muriaux, Jonny Nixon-Abell

Powered by the Eternity technology published in Scientific Reports 

Everspark News

The protocol of use with a glass-bottom dish
The protocol of use with a glass-bottom dish

Community feedback we found interesting


Everspark works with thick samples of 100µ (peripheral blinking).

Testers are currently trying those conditions:

  • Thick samples of 100µ (peripheral blinking)

  • Multiconditions at the same time or sequentially: High Content Screening / High Troughput

We would love to know if somebody is testing  PALM + STORM at the same time.

The community also disclosed that Everspark does not work 100% efficiently with organs and tissues because there is most of the times a bit of remaining oxygen.

Everspark is already cited in these publications

Fluorescent Polymer-AS1411-Aptamer Probe for dSTORM Super-Resolution Imaging of Endogenous Nucleolin
Fabre L, Rousset C, Monier K, Da Cruz-Boisson F, Bouvet P, Charreyre MT, Delair T, Fleury E, Favier A. Biomacromolecules. 2022 May 12. doi: 10.1021/acs.biomac.1c01706. PMID: 35549176

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.
Michalik S, Siegerist F, Palankar R, Franzke K, Schindler M, Reder A, Seifert U, Cammann C, Wesche J, Steil L, Hentschker C, Gesell-Salazar M, Reisinger E, Beer M, Endlich N, Greinacher A, Völker U. Haematologica. 2022 Apr 1;107(4):947-957. doi: 10.3324/haematol.2021.280154. PMID: 35045692

Insights in ChAdOx1 nCoV-19 vaccine-induced immune thrombotic thrombocytopenia.
Greinacher A, Selleng K, Palankar R, Wesche J, Handtke S, Wolff M, Aurich K, Lalk M, Methling K, Völker U, Hentschker C, Michalik S, Steil L, Reder A, Schönborn L, Beer M, Franzke K, Büttner A, Fehse B, Stavrou EX, Rangaswamy C, Mailer RK, Englert H, Frye M, Thiele T, Kochanek S, Krutzke L, Siegerist F, Endlich N, Warkentin TE, Renné T.
Blood. 2021 Dec 2;138(22):2256-2268. doi: 10.1182/blood.2021013231. PMID: 34587242

Superresolution Microscopy of Drosophila Indirect Flight Muscle Sarcomeres.
Szikora S, Novák T, Gajdos T, Erdélyi M, Mihály J. Bio Protoc. 2020 Jun 20;10(12):e3654. doi: 10.21769/BioProtoc.3654. eCollection 2020 Jun 20. PMID: 33659324



High stability over time for repeated measurements

One mounted sample is stable over 3 to 4 weeks and can be imaged multiple times


Multicolor (incl. green)

Compatible with green, red & far red dyes (JF646, JF549, AF647, CF647, DL550, CF568, DL650, CF680, SulfoCy5) and mEos2


Ready to use

Can be used directly from the vial


Up to 6 months performance

Individualized packaging for optimal longevity



HILO, TIRF pattern


Option: customizable

Choose the MEA concentration, pH, optical index, or even additives


Simplified protocol

Storage conditions: 

  • Everspark has to be stored in the fridge at 4°C upon receiving the product. Do not freeze it.
  • Open the tube just before mounting to limit oxygenation
  • Store the slides at 4°C and in the dark before repeating dSTORM acquisition on the same slide.

Use method:

  • Rinse the coverslip with sterile water
  • Discard water in excess
  • Prepare sealant: 1 volume of sealant catalyst + 1 volume of sealant basis in a micro-dish – do not mix
  • Open the Everspark tube and pipette right away the desired volume onto a Wilco glass bottom dish
  • Flip the coverslips face towards the slide on the cavity filled with Everspark without making bubbles
  • Absorb excess of Everspark buffer on the sides with a kimwipe
  • Mix sealant
  • Seal quickly with the reconstituted sealant
  • Wait for the sealant to polymerize on the slide before moving the slide
  • Discard the open tube of Everspark buffer (cannot be used for another slide, once already opened)
  • Gently clean the coverslip with 70% ethanol
  • Perform regular dSTORM acquisition (best to wait a couple of hours)
  • Repeat dSTORM acquisition 24 hours up to 3 weeks on the same slide providing no air bubbles were present
  • Store the slides at 4°C and in the dark before repeating dSTORM acquisition on the same slide (up to 3 weeks)

Note 1: Blinking events have been validated with JF646, JF549, AF647, CF647, DL550, CF568, DL650, CF680, SulfoCy5 and mEos2

Note 2: Blinking is detected for several weeks without loss of events or photons per event

Note 3: Mounting can also be performed in various microdishes by adjusting the Everspark volume (Each vial contains 450 µl)

The recipe to prepare a good sealing

Product Information


Up to 6 months (2 months after opening the pouch + 4 months storage in the pouch).

Kit Description

10 vials with 450 µL or 150 µL of Everspark buffer at 100 mM MEA in Tris
1 vial per experiment


10 vials or your own Everspark
Choose the MEA concentration, volume and pH. See below.

Customizing Everspark

Tune the MEA concentration, the volume or the pH
Ask for a Custom Everspark quote, and our product development team will come back to you.

You want something even more specific?
Contact us and we’ll get back to you with a quote. We’ll do everything to deliver the product that fits your experimental needs!

Everspark and us

We designed the Everspark buffer to facilitate sample preparation for dSTORM super-resolution imaging. Indeed, an oxygen-free buffer containing a thiol reducer is central to promote fluorophore blinking and thus single molecule detection, required for dSTORM imaging. With classical buffers, such as the Glox buffer, fluorophore blinking lifetime is limited to a few hours. With Everspark buffer, it is now possible to increase sample lifetime to several weeks. We demonstrated that a calibrated sample mounted once with the Everspark buffer can be submitted to several rounds of dSTORM imaging to acquire and reconstruct a structure reproducible in coverage and precision, over a period of two months.”

Esther Graudens, New projects at Idylle

“When I was working on long manipulations, I dreamed of being able to stop at certain crucial stages. Everspark is the ideal answer to the problem of many researchers who prepare their samples in a hurry simply because they need to image them right away. The Everspark buffer eliminates this tension: they take the time to image whenever they want, with complete peace of mind. They can also make acquisitions over several days. This will really change the way people prepare their experimentations, and that’s exactly what I like about my job!
In terms of production, I am very proud of the way we have managed to set up a just-in-time production process in our labs in record time. And when researchers ask us to introduce variants, our production teams are able to build and produce very quickly their customized buffer to test it”.


Stampwell stamps imprint arrays of wells in hydrogels in just one time. Nothing more to do than load your samples into the wells and image. The Stampwell shapes have been optimized for the imaging of spheroids/organoids as well as fish embryos or larvae up to 20 dpf.

A technology originally designed by​ Gaëlle Recher for spheroids & organoids. Powered by the Mount(n) technology (UniverSlide) published in Scientific Reports. Optimized by Idylle thanks to the Test Program feedback for zebrafish and medaka

Those little extras that make all the difference:

  • For stamps with more than 10 wells, alphanumeric markers make it possible to quickly identify 1 particular sample.
  • When you move your dish from the incubator to the microscope, the samples remain quietly in their wells (except the Stampwell made for aggregation)
  •  Image your biological samples growing for several weeks in the same dish.

See the technical information about each shape in details.

I successfully managed to use Stampwell with Collagen-1.
Matthieu Opitz • Alveole, France

I use the Stampwell V-shape to track the explants development and the embryoids formation from stem cell aggregates in zebrafish, and it makes my work much easier!
Queralt Tolosa • Centre for Genomic Regulation, Spain

I used a Stampwell to ensure that my zebrafish embryos were positioned correctly under the microscope for spatial and temporal experiments. I am pleased with my Stampwell because it saves me time and provides precision!
Shai Eyal • UCSD, USA

Stampwell has proven to be a very easy to use mounting medium for confocal imaging of fixed and immunostained zebrafish embryos.​
Matthieu Simion • TEFOR, France

Live imaging of beating hearts in early zebrafish embryos using the Larvae 1 Stampwell
Anesthetized 3 dpf embryos expressing a fluorescent biosensor in the heart were mounted in agarose microwells made using the Larvae 1 Stampwell and imaged in ventral view.
Credits: Prof. Juan Llopis – CRIB, Universidad de Vastilla La Mancha, Spain

Utilizing the Stampwell V-shape when turning airway organoids inside out is useful to keep them separated, therefore avoiding clumping and merging.
Signe Lolle • Danmarks Tekniske Universitet, Denmark

Apical-out 10-day human airway organoids imaged in a V-shape Stampwell in comparison with suspension plates
Airway organoids were generated from a human bronchial cell line in hydrogel and transferred either to microwells made using a V-shape Stampwell, or suspension plates, for turning them inside out and imaging.
Credits: Signe Lolle – Danmarks Tekniske Universitet, Denmark

Imprinted V-shape 300µ well in agarose
Soaked in fluorescein, negative rendering
Image credit: (c)Gaëlle Recher – Bordeaux

Cell-filled alginate capsule in the V-shape well, imaged in z
Notice that the focus moving through the well height is easily visible (lines in focus Vs out of focus)
Image credit: (c)Gaëlle Recher – Bordeaux


Improved positioning of 2dpf zebrafish larvae using the Embryo 2 Stampwell
Credits: Dr Rui Monteiro – Institute of Cancer and Genomic Sciences, University of Birmingham, United Kingdom

Heart imaging in a Larvae 1 Stampwell
Top picture: green-heart 3dpf zebrafish larvae placed in ventral view. Middle & bottom pictures: 3dpf zebrafish larvae PFA-fixed, mounted in glycerol and inserted in Larvae 1 Stampwell (anterior to the left). Pictures were taken using a Leica Spectral Confocal SP5.
Credits: Dr. Giovanni Risato, Prof. Natascia Tiso and Alina Ramazanova – Department of Biology, University of Padova, Italy



Compatible with most 35 mm dishes and 6-well plates



Use your stamp dozens of times


Safe for transport

Keep samples at the bottom of the wells, even when a dish is upside dow


Long-term imaging over weeks

Samples lay on the bottom of the well. They can freely grow within it to image multiple times.


Alpha-numerical markers* 

create an organized network of samples to identify the samples for a posteriori experiments
* for > 10 wells Stampwells

Global protocol


Pour liquid agarose


Place the stamp


Reticulate the hydrogel


Remove the stamp

To image, just load your samples and image, sequence, transport at will.

To aggregate, seed your cells & let them aggregate.

V-shape & Embryo 1 specific protocol

   8 steps of the protocol of use, by Gaëlle Recher


Kit description

  • Diameter of the stamp: 26mm
  • 6 shapes of wells to choose freely to fit your experiments. See below.

V Shape 300µ:
. Number of pins: 42
. Depth of the well: 1 mm
. Well bottom diameter: 300µm – Shape: circular
. Well upper side: 1mm*0.5mm – Shape: rectangular

V Shape 500µ:
. Number of pins: 42
. Shape of the pins: V
. Depth of the well: 1 mm
. Well bottom diameter: 500µm – Shape: circular
. Well upper side: 1mm*0.5mm – Shape: rectangular

Embryo 1 (aka Rectangular):
. Number of pins: 35
. Shape of the pins: rectangular
. Length * width of the wells: 2 mm * 0,65 mm

Embryo 2:
. Number of pins: 18
. Shape of the pins: a drop
. Length of the wells: 3.90 mm
. Largest width of the wells: 0.88 mm

Larvae 1:
. number of pins: 5 large pins ideal for the 9-14 dpf zebrafishes + 5 medium pins ideal for the 6-9 dpf zebrafishes + 5 small pins ideal for the 3-6 dpf zebrafishes
. Shape of the pins: fish body and tail
. Length of the 5 large wells: 6 mm, 2.7 for the body and 3.3 for the tail
. Length of the 5 medium wells: 5 mm, 2.25 for the body and 2.75 for the tail
. Length of the 5 small wells: 4 mm, 1.8 for the body and 2.2 for the tail

Larvae 2:
. Number of pins: 10. 5 pins for zebrafish larvae and 5 pins for the medaka larvae on the same stamp
. Shape of the pins: a prism.
. Length of the wells: 10 mm for the 5 long wells and 3 mm for the 5 small wells.
. Depth: 1 mm
. Width at the bottom of the wells: 0.3 mm
. Width of the upper side of the wells: 2 mm

Stampwell, Gaëlle Recher, her team and us

 The Stampwell family of products is born from a technology that I designed, called Mount(n). It is the result of my expertise in imaging a wide range of 3D thick samples. I navigated through many communities (gathered around a biological model), and each time I learnt new tricks to mount samples for microscopy experiments. The Mount(n) technology consists in imprinting an array of wells into a hydrogel (typically agarose), each well receiving a 3D sample (embryos of any species, organoids, tissue biopsies…). The design of the wells prevents the object from moving when the plate or dish is moved, does not apply any mechanical constraints to the sample, and paradoxically also enables the sample to keep growing inside. In a single dish, tens of samples can be imaged with any microscope, for as long as necessary.

Esther Graudens, New projects at Idylle

 Together, we turned the Mount(n) technology into a family of research products. First, we have evolved the shape, the color and the material to offer an ergonomic grip and functionalities that meet the usage characteristics of most applications. Then, as we were about to start the production, the first testers found that the stamp did not fit in all their dishes. Within a few days, together with Gaëlle and our design office, we designed a new shape. And in April 2021, Stampwell for imaging was released with 3 different shapes: 2 V-shapes and the Rectangular one. Soon after that, researchers gave us feedback on the fact that they used their stamp for culture and aggregation as well. Gaëlle had been thinking about it for a while so she started designing it. Camille Douillet joined her to finetune it. At Idylle, we relied on the design process and the production line we had previously built to launch that new stamp dedicated to the aggregation. We managed to release it in 6 months, versus 18 months for the first Stampwells. We hope you will enjoy this new stamp as much as the first ones!”

Combining Stampwell

You may also like to combine Stampwell
with other R&D products


PDMS gets out of the clean room!
The no-brainer solution for all your micro-volume experiments, anywhere.

 What is it intended for? Use it notably for wound-healing assay & immunocytochemistry experiments. It is very helpful when you are working with super expensive reagents or very rare cell lines, and when you want to test various experimental hypothesis.
 No limit! Choose a ready-to-use design (Solo – Quartet – Nonet – Presto – Allegro). Or design your own shapes & stack them freely.
 The “Starter kit” provides 2 sheets of 50 Stencells each. Kit descriptions, designs, Technical datasheet and FAQ



Designed by Vincent Studer, Pierre-Olivier Strale and Aurélien Pasturel
They were hosted by the Cell Organ-izers joint research laboratory (CNRS-Alvéole).

Powered by the Stencil technology published in Advanced Healthcare Materials .

Stencell news

Stencell for wound-healing experiments

COS-7 cells colonizing a gap induced by the removal of a PDMS stencil.
Images shot with a 20X objective during 18 hours (1 image every 2 min) with an inverted microscope.
Image credit: 
© Pierre-Olivier Strale

Stacking the 5 designs of Stencell



Design your PDMS
Our design process offers total freedom to turn your idea into a PDMS.

We produce it with the highest standards of quality, whether you want 1 or 100 copies


Stack and play

Compose flow channels and chambers, stacking different designs of Stencell


Stick it to a variety of culture labware

So far, it has been successfully stuck on:

Glass slides

Plastic dishes

Transwell(R) inserts

Polyacrylamide gels


Remove it

Stencell is not glued. It can then be removed to:

Trigger cell migration

Switch from flow chamber to open window

Very useful for wound healing experiments


It is compatible with imaging

These thin sheets of silicone are fully transparent, without autofluorescence


Parallelized and standardized experiments

One multiwell Stencell can perform several experimental conditions


It saves samples and reagents

A few microliters only are required

Gallery of results

Simplified protocol

Storage conditions 

  • The Stencells are packaged between 2 protective foils: if you wish to store them, keep them between these foils, in order to avoid dust deposition.
  • Storage at room-temperature, stable up to 2 years.

Key conditions of success

  • When you remove the inner elements of your Stencell, use 2 tweezers: 1 to remove and the other 1 to press close to the junction in order to avoid breaking the Stencell. Alternatively, you can cut the junctions with a scalpel.
  • When you put the Stencell on top of your culture substrate, softly remove the bubbles and stick the Stencell, patting it with tweezers
  • Depending on the substrate hydrophobicity, the droplet might not completely fill the well. You can:
    * Either fill the well with the standard volume. Then, help the droplets stick to the Stencell by connecting the liquid next to the Stencell walls using the pipette tip.
    * Or, you may add an excess volume of liquid (e.g. 30 μL) and remove it afterwards (e.g. 10 μL).
  • But: do not overfill (e.g. > 30 μL) the wells, otherwise two droplets may merge.
  • When you put your substrate and cells in the incubator. Wait for 1 to 2 hours until cells spread and fully occupy the windows space. If you need to wait longer, be sure  that the droplets do not completely evaporate

Protocol of use

  • Wound-healing experiment in Stencell chambers
    1)     Using tweezers, discard the windows of a Stencell
    2)     Using tweezers, grab the Stencell and place it on your dedicated cell culture substrate (glass coverslip, plastic Petri dish, multiwell plate)
    3)     Optional: use a flat surface to make sure the Stencell properly stick to the cell culture substrate.
    4)     Immerse the sample in complete cell culture medium and place it in an incubator (37 °C, 5 % CO2).
    5)     Detach your cells according to your standard procedure, centrifuge.
    6)     Remove the bubbles present at the interface Stencell/cell cuture substrate using a pipette.
    7)     Seed the cells and place the sample back in the incubator until cells are spread and fully occupy the windows space.
    8)     Carefully remove the Stencell using tweezers while maintaining one corner with a pipette.
    9)     Optional: Place the glass coverslip on its dedicated holder.
    10)   Image with a microscope at 37 °C, 5 % CO2.
  • lmmunostaining of cells confined in Stencell chambers
    1)  Using tweezers, discard the windows of your Stencell
    2)  Using tweezers, grab the Stencell and place it on your dedicated cell culture substrate (glass slide, plastic Petri dish, multiwells plate)
    3)  Optional : use a flat surface to make sure the Stencell properly stick to the cell culture substrate
    4)  Immerse the sample in complete cell culture medium and place it in an incubator (37 °C, 5 % CO2)
    5)  Detach your cells according to your standard procedure, centrifuge.
    6)  Remove the bubbles present at the interface Stencell/cell cuture substrate using a pipette.
    7)  Seed the cells and place the sample back in the incubator until cells are spread.
    8)  Fix with PFA 4 % for 12 min, rinse 3 times with PBS.
    9)  Carefully remove the Stencell using tweezers while maintaining one corner with a pipette.
    10)  Stain the cells with standard procedures.
    11)  Optional: mount the glass coverslip on a glass slide.
    12)  Image on a fluorescence microscope.

Product Information

Kit Description

The “Starter kit”
– 2 sheets of 50 Stencells each
– Either the same design for the 100 Stencells, or 2 different designs (one design per sheet).

The “Get your own design” kit:
– 1 design study to develop your project
– 2 sheets of 50 Stencells each (priced as the ones available in the “Starter kit”)
– You can mix 1 sheet of your design with 1 sheet of the already existing ones.

The designs:
Each sheet is composed of 50 identical Stencells.
Upon receipt of your purchase order, you will be able to choose either the same design for your 2 sheets, or 2 different designs for your 2 sheets (1 per sheet of 50).

– Solo: 1 circular well – Diam. 12 mm
– Quartet: 4 circular wells – Diam. 3 mm
– Nonet: 9 circular wells – Diam. 3 mm
– Presto: 2 oblong wells spaced by 0.35 mm
– Allegro: 2 oblong wells spaced by 0.62 mm


The Stampwell most Frequently Asked Questions

Stencell and us

“While developing an innovative micropatterning technology on glass coverslips, we had to optimize our protocole and thus screen a lot of conditions with expensive reagents. We could have used glass-bottom 96wp but we found them expensive and inconvenient for reproducible surface treatment. We thus started to develop our own low-volume fluid handling method. We quickly discovered that hydrophobic perforated PDMS films could act as reversible boundaries for liquids. With simple craft cutting robot, we were able to fabricate on-demand stencils of any shape and size. By filling those stencils with microliters droplets, we end up with a very versatile and reproducible high-throughput fluid handling solution.”

Esther Graudens, New projects at Idylle

“Sometimes research needs a high level of versatility. This is exactly what appealed me when I met Vincent, Pierre-Olivier and Aurelien. They were hosted by the Cell Organ-izers joint research laboratory, set up by CNRS and our sister company Alveole. So they naturally turned to us when they decided to look for a partner to release their stencils. We knew that Stencell would be so useful to a lot of researchers who could save reagents and cells while testing new ideas and experimental conditions on small volumes. So we said yes immediately. On top of that, the three of them have proven to be highly reactive and proactive, engaged and enthusiastic. They love as much as we do testing new ideas and suggest others. It was a great pleasure to go through our industrial process together!”


Calibrate your blinking experiments!

SpheroRuler is a monodispersed suspension of 1µm diameter spheres coated with 647-fluorophores giving a stable blinking in SMLM microscopy. Their consistent size and geometry make them very practical and reliable standards to assess the accuracy of your x-y or z measurements, 3D reconstruction methods or your image quality. Monodispersed and immobile in solution, they can also be used as rulers, for drift correction or as guides to help localize features on your biological samples.


SpheroRuler is great for dSTORM calibration experiments because you can use it on your preferred support and buffer. And because you get confident with the new biological structure to image. SpheroRuler also produces simple shapes that are easy to study when you are a dSTORM beginner!

How do you know your imaging process is good with SpheroRuler?  Your beads blink, you reconstruct a ring in 2D and a sphere in 3D experiments, and you check that you retrieve the 1 µm diameter.

A technology designed by Arnaud Favier, Karine Monier and Christophe Place.


Stable blinking

Suited for use in SMLM microscopy


Consistent size

Thoroughly characterized by electron microscopy


Spherical geometry​

with sharp and thin edges



Suitable for drift correction applications



Resuspended in an aqueous buffer and usable along biological samples such as cells or tissue sections



A high fluorescence intensity for practical use as demo or training tools

Gallery of results

SpheroRuler beads imaged using SRRF-Stream super-resolution microscopy
(A, B). Pictures of SpheroRuler beads acquired in wide-field (A) and SRRF-Stream super-resolution (B) imaging. Scale bar = 5µm (C, D) Local enlargements of A and B pictures respectively.
Scale bar = 1µm (E) Fluorescence intensity distributions along the solid lines in C and D.
Credits: Yao Baoli – Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, 2023

2D and 3D dSTORM images of a SpheroRuler bead acquired with a Leica GSD system
Credits: Lydia Y Li, Nabi Lab, The University of British Columbia, 2023

Technical information

Kit Description

Kit description
1 vial of 50 µL of a SpheroRuler suspension in PBS pH7.4.

Concentration: 7.10exp8 particles per mL.

Allows 10 experiments when using the recommended 5µL volume in 35mm glass-bottom dishes.

Stability: up to 7 months when stored at 4°C.

Dye: 647-fluorophore (far-red fluorescence).

Compatible with: dSTORM, SRRF, Airyscan confocal, confocal, SEM


Q/ What are SpheroRuler beads made of?
The SpheroRuler beads are 1μm-diameter polymer particles surrounded by 647-fluorophores
covalently anchored to their surface. Beads will be visible as hollow rings or spheres when
reconstructed in 2D or 3D SMLM experiments respectively.

Q/ Which types of imaging is SpheroRuler compatible with?
SpheroRuler beads are coated with 647-fluorophores giving a stable blinking in SMLM
micrsocopy, and have been initially developed for dSTORM imaging. Since then, they have also
been successfully used in SFFR, SEM, confocal, Airyscan confocal and SEM microscopy.

Q/ How accurate is the SpheroRuler bead size?
The spherical particles making up Spheroruler beads have been selected based on very good
monodispersity properties. The accuracy and reproducibility of the bead diameter have been
characterized by SEM on 25 independent microspheres and showed a standard deviation of
1 +/- 0.05μm.

Q/ What is included in the SpheroRuler kit? Is there anything I need that is
not provided?
The SpheroRuler kit contains a 50μL suspension of SpheroRuler beads. All you need to have
on your side is some blinking buffer, coverslips and imaging vessels of your choice.

Q/ How many experiments can I carry out with one SpheroRuler kit?
One kit contains a 50μL suspension of SpheroRuler beads, allowing for 10 experiments when
using the recommended 5μL volume in 35mm glass-bottom dishes.

Q/ Can I use it alongside my biological samples?
Yes, SpheroRuler beads are resuspended in PBS and can be loaded together with biological
specimens (cells, tissue sections, etc).

Q/ For how long can I keep my SpheroRuler solution?
The SpheroRuler suspension is stable for at least 7 months when stored at 4°C.

Q/ Is there any specific reconstruction algorithm I should use?
SpheroRuler beads are highly fluorescent beads coated with a high density of fluorophores.
We recommend using a multi-emitter fitting approach, or any equivalent, to guarantee an
efficient localization of individual blinking events. Although the available options will vary on
each imaging system used, examples of algorithms that were successfully used include the
“account for overlap” function on the ZEN software, or the “high-density”mode on the Zeiss
software and the freely available UNLOC software.

Q/ How should fluorophore thickness be taken into account in the diameter
Fluorophores are directly coated on the bead surface without linkers or antibodies, and their
thickness is therefore negligible compared to the measured diameter. The apparent thickness
of the fluorophore ring will depend on the resolution of your imaging system (i.e. around 160-
200nm when measured in dSTORM). The external periphery of the beads should be taken into
account when measuring diameter.

Q/ I am not retrieving a perfectly spherical shape when reconstructing the
beads in 3D. Is it normal?
Although 2D reconstructions of SpheroRuler beads should be accurately circular, obtaining an
elongated shape in z is a common artefact that will depend on the system used for imaging
and for 3D reconstruction (biplane reconstruction, astigmatism, etc). The measured diameter
in z and its distance from the actual 1μm can therefore be used as a robust indicator to
evaluate the fidelity of the 3D reconstruction for a given system. As an example, measured z
diameter was 1.3 μm when tested on a Vutara VXL system using biplane imaging.


“It could have been one of those after-dinner conversations that would have ended there. Arnaud, Karine, Christophe and Mathieu, from Idylle, had a lively discussion about some of our experimental conditions in dSTORM. They came to talk about the confidence we can place in our instruments. Mathieu launched: “You should develop a calibration tool for dSTORM, it would be of great help for so many researchers”. This little phrase hung in the air… until we decided that it was a challenge that we liked and that we were going to take up. Today we are happy to launch SpheroRuler with the whole Idylle team.”

“Calibration beads for dSTORM? Why hadn’t we thought of that before? Their very simplicity was praised by early researchers who tested and adopted them. Scientists new to SMLM greatly appreciate their ease of use. We are all very happy here that this idea of a night has become a research tool for all “.


AgarSqueezer is a microscope slide chamber equipped with a molded agar-based compression system. Use it to assess cell response to short and long-term mechanical confinement within a physiological rigidity range.

It is very helpful if you want to analyze how your cells will react if you squeeze them for a prolonged period. Or if you want to study how mechanical confinement affects drug cell resistance. And if you want to perform immunostaining in situ.

Designed by Audrey Prunet, Gilles Simon, Hélène Delanoë-Ayari, Véronique Maguer-Satta and Charlotte Rivière who tell the story of their transfer here .

Published in Lab on a chip

Agarsqueezer has been successfully used to confine adherent and non-adherent cells, including:

Human: primary T-lymphocytes, TF1 & ML2 leukemic cells, HS27A fibroblasts, MCF10A breast cells, MDA-MB-231 breast cancer cells, U-2 OS osteosarcoma cells, PC-3 & DU 145 prostate cancer cells, HT29 & HCT116 colorectal adenocarcinoma cells, HT1080 fibrosarcoma cells and megakaryocytes
Murine: osteocyte-like cells MLO-Y4, primary muscle cells & primary dendritic cells
Plant: cells from Arabidopsis roots
3D cell objects: mice gastruloïds

Compression of mouse primary myoblasts using AgarSqueezer
C57 primary myoblasts stained with Hoechst were imaged in the AgarSqueezer before compression (left panel), and after 1.5h of compression under 2.5 µm height pillars (middle & right panels).
Credits: Dr. Hind Zahr & Dr. Alice Varlet, Lammerding Lab – Meinig School of Biomedical Engineering, Cornell University, United States

Arabidopsis root cells confined using Agarsqueezer

Arabidopsis thaliana Col-0 root cells stained with Calcofluor (cell wall) and imaged with a confocal microscope either in a traditional culture setting (left), or after 24h of confinement in the Agarsqueezer using the 30µm (middle) or 5µm (right) pillars.

Credits: Léa Bogdziewiez – UPSC – Sveriges lantbruksuniversitet, Sweden


Tunable stiffness in a physiological range [1-150] kPa

Use of agarose as a cheap and biocompatible polymer


Open access to the reservoir

Possibility to add drugs, and reagents

Easy medium renewal


Autoclavable & reusable systems


Compatibility with multiple microscopy techniques

Confocal, spinning, super-resolution

Open access for microscope objectives

Use of optical glass coverslip to make cells grow


Easy to recover coverslip with cells for subsequent molecular analysis

FACS, qPCR, Western-Blot, Immunofluorescence (possible in situ)


Long-term analysis of the cell adaptation to confinement

Up to several days, for time-lapse studies


Study of the specific impact of mechanical loads on the biology of cells

Gas permeability of the system allows to get rid of the hypoxia conditions


Easy to assemble and disassemble the system

AgarSqueezer – How to use it – A piece of protocol in 3 steps


Check the most Frequently Asked Questions (FAQ).

Kit description

You can choose among 2 kits:
– The “1 AgarSqueezer kit” contains 1 AgarSqueezer device
– The “2 AgarSqueezers kit” contains 2 AgarSqueezers devices.

In addition to the device, each kit contains also:
– 1x insert to hold up to 2 AgarSqueezers on the microscope stage
– 1x 16G flat cut needle to make holes in the agar gel, facilitating diffusion of culture medium or drugs during the experiment
– 1x 20G flat cut needle (same)

For users who also need a wafer to mold agarose, we can provide it in 4 different heights:
. 2.5 µm to highly confine cells
. 5 µm to moderately confine cells
. 30 µm not to confine cells (negative control)
. 100 µm for 3D confinement

“In the beginning, in Charlotte’s team, we wanted to analyze the influence of both stiffness and confinement on cells, mimicking highly confined situations such as fibrosis or cancer.
On its side, Veronique’s team was looking for a way to analyze the effect of long-term confinement with the ability to add a drug at any time.

We all wanted to find a device that would also meet the requirements of subsequent classical molecular analysis (easy cell culturing and cells recov​ery, qPCR, western-blot, in situ immunostaining) as well as biophysical image-based analysis (high-resolution microscopy and video-microscopy). But we could not find any.

So all together, we decided to collaborate on the perfect set-up. We rapidly identified agarose as an interesting material to get medium and oxygen renewal, with no drug adsorption. We built several prototypes, improving precise control of the confinement, avoiding destruction of the gels and leakage of the culture medium! And then we came up with the Softconfiner device, that we published and decided to transfer.
Today, we believe that the AgarSqueezer could be of interest for many researchers willing to better understand how mechanics can regulate cell behavior. We hope that researchers from different communities will also find it a useful tool!”

Audrey Prunet, Gilles Simon, Hélène Delanoë-Ayari, Véronique Maguer-Satta and Charlotte Rivière


“When Charlotte showed me for the first time their Softconfiner device, I got highly impressed by its level of maturity. The team of developers had managed to confine cells up to 8 days without affecting the cells viability, but had observed that mechanical confinement affected the cell proliferation of all the tested cell lines. Charlotte and the team were very clear on their motivation to make their Softconfiner easily accessible to the research community. In addition to being a pleasure to work together, it was quite obvious the technology would become a great product.” Esther Graudens
New projects at Idylle

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