For over 37 years Jackson ImmunoResearch have specialised in the manufacture of secondary antibodies, conjugates and purified immunoglobulins. Their products are made by scientists for scientists and are considered by many to be the gold standard. With their state of the art production facility in rural Pennsylvania and their European hub in Cambridgeshire they serve Research Institutes and Universities throughout the world.


His-Tag Offer
50% OFF Anti-Rabbit Antibodies
His-Tag Offer
Lateral Flow
Lateral flow immunoassays are vital tools for surveillance and screening
Lateral Flow
Gold Conjugates
NEW 40 nm Gold Conjugates
Gold Conjugates
VHH
Choose the right tools for developing and screening VHH antibodies
VHH
previous arrow
next arrow

Product Categories

Technical Centre

Download the JIR Catalogue, Product Handouts, White Papers and Posters

New! Anti-Camelid Secondary Antibodies

At Jackson ImmunoResearch, our mission is to develop the widest range of high quality secondary antibodies for the life science community. To support the growing adoption of camelid-derived heavy chain variable domain (VHH) technologies in a multitude of applications, including next-generation immunotherapeutics, medical imaging, analytical and diagnostic assay development and research discovery, JIR offers three new secondary antibody specificities for detection and quantification of camelid antibodies.

Immunoglobulins Poster

Download our guide to Immunoglobulins Structure and Function

Multiple Labelling Poster

Download our guide to Multiple Labeling with Secondary Antibodies

Flyers

Guides

See JIR Secondary antibodies in action

Contact marketing@stratech.co.uk for more information on any of the following images.

Anti-Alpaca IgG and VHH domain Secondary Antibodies

Find out about our Secondary Antibodies

Mouse on Mouse Labeling with Fab Fragment Blocking

Anti-Camelid Secondary Antibodies

Having manufactured and supplied secondary antibodies and related reagents for 34 years, Jackson ImmunoResearch know the best practices to employ, as well as the pitfalls to be avoided in their use. In these articles we offer the benefit of our experience with useful and practical advice.

Featured Products

Product Code Product Description
115-545-003 Alexa Fluor® 488-AffiniPure Goat Anti-Mouse IgG (H+L)
115-035-003 Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L)
115-545-166 Alexa Fluor® 488-AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Rat Sr Prot)
715-545-151 Alexa Fluor® 488-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Rat,Shp Sr Prot)
715-035-151 Peroxidase-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Rat,Shp Sr Prot)
111-545-003 Alexa Fluor® 488-AffiniPure Goat Anti-Rabbit IgG (H+L)
111-035-003 Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L)
111-545-144 Alexa Fluor® 488-AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot)
111-035-144 Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot)
711-545-152 Alexa Fluor® 488-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot)
711-035-152 Peroxidase-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot)

We can manufacture and supply most standard inventory secondary antibodies in bulk volumes upon request.

Find out more about Bulk Services below;

We can manufacture and supply most standard inventory secondary antibodies in bulk volumes upon request.

The combination of our experience, our focus on Secondary Antibodies and our core principle of manufacturing to the highest quality standards, ensures that customers can be completely confident of product quality, consistency and minimal lot to lot variation over the long-term. These factors contribute to the efficiency of sample evaluations and approval of bulk materials, and to our goal of providing the highest quality of service.

Jackson ImmunoResearch Laboratories, Inc. is certified by BSI to ISO 9001:2015 under certificate number FM 545248.

Our Expertise

  • Over 30 years of experience in a single, highly specialized field; Secondary Antibody and Conjugate manufacture and supply
  • Long-term customer relationships as an established and reliable supplier
  • Fast, efficient customer services
  • Maintaining the highest standards in our research, manufacturing and all operations, which contribute to ensuring guaranteed product quality, long term product consistency and reliable performance.

Our Antibodies and Related Products

We manufacture the most comprehensive range of secondary antibodies and related products. Antibodies are raised in a selection of host species and directed against a wide variety of species and immunoglobulin regions.

All our antibodies are available unconjugated, or conjugated to enzymes (HRP and alkaline phosphatase), a wide range of fluorophores, as well as biotin.

Whole IgG Secondary Antibodies

Specificities include:

  • Whole Ig
  • F(ab’)2 region
  • Fc portion and subclasses
  • Light chain

F(ab’)2 Fragment Secondary Antibodies

Fc region is removed, avoiding binding to live cells with Fc receptors, Protein A or Protein G.

Fab Fragment Secondary Antibodies

Monovalent antibodies for blocking or multiple labeling involving primary antibodies from the same host.

Purified Serum Proteins

For use as experimental controls.

Normal Serums

Blocking agent to reduce background from non-specific, conserved sequence, and/or Fc receptor binding.


Terms of Sale

  • Quotation: JIRE will issue an official quotation including product quantity, pricing, and delivery terms.
  • Order Confirmation: Receipt of the order by JIRE is confirmation of acceptance of terms. An order confirmation from the customer must be received in writing.
  • Purchase Requirements: Customer agrees to purchase and accept shipment of listed quantity at the quoted price. Minimum order quantities apply.
  • Standing Orders: Standing orders are accepted with pre-set ship dates. The customer agrees to purchase the entire quantity of the standing order. There can be no changes to quantity and shipment dates, unless agreed to by JIRE. Each standing order is valid for no more than 12 months from order date unless approved by JIRE.
  • Packaging and Fill Volume: In the event the packaging fill volume differs from Jackson ImmunoResearch Labs, Inc (JIR) standard product fill volume, JIR will provide, on request, samples of the product. Customer agrees to purchase samples at quoted price. Upon sample approval, JIR will deliver the quoted bulk quantity. Customer agrees that the bulk quantity is not eligible for returns or refunds.
  • Product Performance: Upon product acceptance, customer assumes responsibility for product performance.
  • Terms of use: Products are designed for research use only. Customer is responsible for qualifying products for intended use.
  • Additional Terms: JIR will make every attempt to meet expedited requests regardless of the scheduled release date within the limits of material availability and capacity.
  • Payment terms: To be agreed

FAQs

1. How do you determine protein concentration?

Jackson ImmunoResearch uses the Biuret assay to determine protein concentration.
If protein concentration is obtained by using the mass extinction coefficient of 1.4 for a 1 mg/ml solution at OD280, the result will be 10-15% lower than the concentration obtained by the Biuret assay.

2. What is the molecular weight of my product?

Jackson ImmunoResearch does not determine molecular weights by analytical methods. An approximate molecular weight of 160, 110 and 50 kDa can be used for whole IgG, F(ab’)2 and Fab fragment antibodies, respectively. To obtain an estimated molecular weight of any other product of interest, please contact Technical Service at help@jacksonimmuno.com.

3. Do you make custom antibodies?

For purchase of OEM products that have custom specifications (e.g. custom adsorptions, conjugations), please contact Technical Service at technical@stratech.co.uk. Jackson ImmunoResearch does not provide polyclonal or monoclonal antibody development or production services.

4. Are your products endotoxin-free?

Jackson ImmunoResearch does not assay products for endotoxin content. For applications requiring low endotoxin consider using an endotoxin removal column, available from Thermo Fisher, Charles River Laboratories, and other suppliers.

5. Why is there a discrepancy regarding the protein concentration, volume of dH2O used to reconstitute the lyophilized powder, and the nominal fill size reported on the spec sheet?

For products that are sold by weight (mg), Jackson ImmunoResearch fills vials with slightly more product that the nominal mg amount (“Size” on the spec sheet). To calculate the actual product amount in a vial, multiply the protein concentration indicated on the spec sheet by the volume of dH2O needed to rehydrate the lyophilized pellet.

1. What are common causes of background?

Background may be caused by the primary antibody. Perform a control experiment excluding the primary antibody to isolate the secondary antibody as the cause of background.
Improper blocking of the tissues or cells.
Cross-reactivity of the labeled secondary antibodies with endogenous immunoglobulins on the tissues or cells.
Inadequate washing.
Reactivity of the labeled secondary antibody with immunoglobulins in the diluent.
Not diluting the secondary antibodies far enough.

2. What are some causes of weak signal?

There is insufficient antigen present and an amplification protocol may be needed.
Change from a more sensitive detection method to a less sensitive detection method.
The primary antibody is diluted too far.
Enzyme activity is inhibited.
The secondary antibody does not recognize certain primary antibodies well.
The secondary antibody cross-reacts with immunoglobulins in the diluent.

3. What diluent is recommended for the secondary antibody?

The antibodies can be diluted in buffers such as PBS or TBS. A detergent such as Tween 20 (0.05% v/v) can be included to reduce non-specific binding. We recommend making the working dilution fresh on the day of use. Carrier proteins such as BSA or normal serum are not necessary, and in some cases can increase background staining due to protein-protein interactions.
Read more about diluents in our guide to blocking, controls and diluents.

4. What blocking buffer is recommended?

For most applications, we recommend blocking with normal serum (5% v/v) from the same species as the host of the secondary antibody. Other commonly used reagents such as BSA and non-fat dry milk are suitable for some applications, but may increase background if the secondary antibody is directed against goat, sheep, or horse.
Read more about blocking in our guide to blocking, controls and diluents.

5. What incubation time is recommended for the secondary antibody?

Incubation times of 30-60 minutes are recommended for surface interactions such as ELISA or Western blotting. For staining tissue, incubation times should be increased as necessary to allow for tissue penetration. Total wash time should be approximately equal to incubation time to allow unreacted antibody to diffuse out.

1. What is an antibody subclass?

Immunoglobulin classes (IgA, IgD, IgE, IgG, IgM in mammals) are defined by the structure of their heavy chains. Minor differences on heavy chains are found between subclasses of each class. Jackson ImmunoResearch offers polyclonal antibodies that are specific for mouse subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. We also have antibodies that are specific for camelid subclasses IgG 2+3. We currently do not have subclass-specific antibodies against other species.
Download Immunoglobulin Structure and Function Poster.

2. What are the differences between Fab, Fc, and F(ab')2 fragments?

These products are the result of proteolytic digestion of IgG. Digestion with papain results in Fab and Fc fragments, and digestion with pepsin yields F(ab’)2 fragments.

3. What is an antibody isotype?

Isotypes are distinct forms of antibody heavy and light chains, and may refer to class or subclass. Light chain isotypes are kappa and lambda, and heavy chain isotypes are alpha (IgA), delta (IgD), epsilon (IgE), gamma (IgG), mu (IgM). The heavy chains may be further differentiated as subclasses, e.g. gamma 1 (IgG1).

4. What is an isotype control?

Isotypes are distinct forms of antibody heavy and light chains, and may refer to class or subclass. Light chain isotypes are kappa and lambda, and heavy chain isotypes are alpha (IgA), delta (IgD), epsilon (IgE), gamma (IgG), mu (IgM). The heavy chains may be further differentiated as subclasses e.g. gamma 1 (IgG1).
An isotype control is a negative control that verifies specific binding of either a primary or secondary antibody. The isotype control is the same class or subclass as the experimental antibody, but is not specific for the antigenic target. Isotype controls may be purified IgG from normal serum of the species host of the experimental antibody; or in the case of monoclonal antibodies may be monoclonals of the same subclass, directed against an irrelevant antigen. Isotype controls for unlabeled antibodies are unlabeled, and controls for labeled antibodies are labeled with the same reporter molecule (fluorophore, enzyme, or biotin) as the experimental antibody.


50% OFF Anti-Rabbit Antibodies

Anti-His Tag Summer Offer

Buy our Anti-His Tag Antibody and get 50% off any Anti-Rabbit Antibody NEW Anti-His Tag antibodies from JIR are suitable Read More...

Serological testing for diagnostics and disease surveillance

Serological tests enable disease surveillance from initial infection through to the development of immunity against infectious diseases. The power of Read More...

Lateral flow tests for diagnostics

Lateral flow assays are one of the many types of immunoassays available for health monitoring. As point of care diagnostic Read More...

Selecting Fluorophores for Antibody-based Research

Fluorophores are essential tools for scientific research. They are widely used for immunoassay techniques such as flow cytometry, immunohistochemistry (IHC), Read More...

An Introduction to Secondary Antibodies

Antibodies are invaluable to scientific research, diagnostics, and therapeutics. These simple yet powerful scientific tools find their way into a Read More...

Solutions for Species on Species Experiments

When using primary antibodies derived from the same species as the sample material (species on species)  non-specific background staining can Read More...

Cross-adsorbed secondary antibodies and cross-reactivity

Immunoglobulins from different species share similar structures. Secondary antibodies raised against one species are likely to recognize epitopes on other Read More...

Blocking WIth Fabs

Blocking: Use of unconjugated Fab fragments to block endogenous immunoglobulins and avoid off target signal   Background staining may be Read More...

Anti-Chicken Antibodies

Conjugated Anti‑Chicken antibodies are the detection partner for chicken‑derived antibodies, a host species gaining popularity as a component of Lateral Read More...

Anti-Human Secondary Antibodies

Jackson ImmunoResearch AffiniPure™ Anti-Human secondary antibodies are affinity purified polyclonal antibodies with specificity for human immunoglobulins. We offer Anti-Human secondary Read More...

Western Blot

Western blotting with JIR secondary antibodies Western blotting is an analytical technique used to detect specific proteins or peptides in Read More...

Alexa Fluor Secondary Antibodies

Alexa Fluor® fluorescent dyes are widely recognized as superior fluorescent dyes available for conjugation. They are highly water soluble and Read More...

Brilliant Violet

BD Brilliant Violet™ 421 and 480 Conjugates Add more color to your immunofluorescence with our NEW Brilliant Violet™ conjugates Brilliant Violet 421 and Brilliant Violet 480 conjugated Read More...

serology

Immunoassays for diagnostics testing Serological tests are key assays for many health issues, including solid organ transplantation screening, crossmatching, disease Read More...

Lateral Flow Tests

Lateral Flow Rapid assays like lateral flow tests provide quick yes or no results in an easy to use format. Read More...

Fluorescent Western blotting

Fluorescent Western blotting for quantitative and multiplex detection Fluorescent Western blotting allows multiplex detection without stripping and reprobing for analytes, Read More...

Anti-Fc Specific Antibodies

Heavy chain specific detection on Western blots after IP with Anti-Fc specific antibodies Anti-IgG, Fc fragment specific antibodies may be Read More...

Anti-fade

Recipe for Anti-fade Mounting Medium In our experience n-propyl gallate added to mounting media reduces fading of fluorescence from many Read More...

Silver Enhancement Protocol

Caution: Purity of water and cleanliness of glassware are crucial for optimal silver-staining results. Also, contact between metallic objects (for Read More...

Blocking and labeling with Fab fragments

Monovalent Fab Fragment Affinity-Purified Antibodies for Blocking and Double Labeling Primary Antibodies from the Same Host Species Monovalent Fab fragments Read More...