Founded in 2006, Affinity Biosciences is a pioneering Life Science and High-Tech company whose biochemical products and services are mainly applied in biotechnology, medical research and pharmaceutical development.


Product Categories

Primary Antibodies

Antigen Peptides

Inhibitors

Loading Control & Tag Antibodies

Reagents & Kits

Congratulations to Affinity Biosciences for becoming the first company to ever win CiteAb Awards 'Antibody supplier to watch' a second year in a row! Both 2020 and 2019!

Quality comes first, result always counts

Quality assurance at Affinity Biosciences is of utmost significance that Affinity Biosciences cannot afford to lose. Our quality peptide design is essential to generating specific, selective, and reproducible antibodies first thing. Affinity Biosciences uses synthetic peptide haptens or recombinant protein to produce antibodies with expertise and proficiency from the beginning, thereby allowing product quality to be under close control while enabling us to target particular protein regions and isoforms.

More than antibodies

Aside from being the major supplier of innovative tools for signaling transduction research, Affinity Biosciences also offers a vast variety of more than 500 inhibitors and SiRNA. Affinity Biosciences also plays an active role in customer service of antibodies and peptides.

Solution through innovation

Affinity Biosciences is striving to bring about constant cooperation with numerous renowned pharmaceutical and biotechnological companies and academic institutions, advancing their research efficiency and core competitiveness and ultimately serving humanity.

Affinity Biosciences offer custom services in the following areas;

  • Custom Polyclonal antibody
  • Custom Phospho-Specific antibody
  • Custom Modification Specific Antibody
  • Peptide Synthesis Service
  • Inhibitors & Organic Synthesis

Affinity-Purified Polyclonal antibody production (From Peptide Synthesis to ELISA titer assay)

Polyclonal Antibody Development Schedules

Phase Time

I. Peptide synthesis

Peptide synthesis up to 13-15 amino acids.

2 week

II. Conjugation

Conjugation of 10 mg peptide (using Cysteine) to KLH.

10 mg free peptide will be provided for client

1 week

III. Immunization and anti-sera production

Eight-week protocol to produce antibodies in 2 rabbits.

Pre-immune 5 ml/per rabbit and total final bleeds from two rabbits (50 ml antiserum) will be provided to client.

8 weeks

Ⅳ.Peptide affinity purification and ELISA test.

5 mg specific antibody will be sended to client,the antibody tilter will be 1:32000+

2 weeks
Total 13 weeks 

Notes
All the products of this project shall become the exclusive property of the client at the conclusion of the project.

Full confidentiality is guaranteed

Affinity-Purified phospho-specific polyclonal antibody production (From Peptide Synthesis to ELISA titer assay)

Polyclonal phospho-specific Antibody Development Schedules

Phase Time

I. Peptide synthesis

1. Phosphospecific peptide synthesis (up to 15 amino acids)–10 mg used for phosphospecific epitope affinity purification, 10 mg used for antibody production, and 10 mg delivered to client 2. 10 mg non-phosphospecific peptide (up to 15 amino acids)used for affinity purification.

2 weeks

II. Conjugation

Conjugation of 10 mg phosphopeptide (using Cysteine) to KLH and BSA(for pep-elisa), 5mg non-phosphopeptide(for pep-elisa).

1 week

III. Immunization and anti-sera production

Eight-week protocol to produce antibodies in 2 rabbits. Pre-immune 5 ml/per rabbit and total final bleeds from two rabbits (50 ml antiserum) will be provided to client.

8 weeks

Ⅳ.Peptide affinity purification and ELISA test.

Separating the phosphorylated antibodies from the non-phosphorylated antibodies is performed by immuno-affinity purification, using two columns ,which does not cross-react with non-phosphospecific peptides (confirmed by ELISA)

2 weeks
Total 13 weeks

Notes
All the products of this project shall become the exclusive property of the client at the conclusion of the project.

Full confidentiality is guaranteed

Post translational Modification (PTM) is a key component that contributes to the diversity of the biological activities and affecting many protein characterization or cellular functions. Affinity bioscience raised against these protein modifications has shown to be an useful tools in the study of cell signaling, protein structure, function, maturation, localization, disease diagnostic, activity, etc.

While protein phosphorylation,Aceytylation and methylation is considered the most common post translational modification, more than 200 different covalently attached modifications targeting most proteinogenic amino acids are known today. Affinity biosciences has been an expert in providing custom-made modification specific peptides as antigens for antiserum production. All antibodies against post translational modifications will undergo double affinity purification with modified and unmodified peptide.

Note;
The first step in the development of custom-made modification-specific antibodies is the selection and design of the immunizing peptide. This is a crucial step because the specificity and utility of the custom modification-specific antibodies depends critically on the design of the immunizing peptide. Affinity biosciences provides design assistance at no additional charge. Please send your sequence to our support team with any details on regions to target/avoid, and we will be happy to send our recommendations. Once the final sequence has been confirmed, we can then put together a formal quotation for the custom modification–specific antibody project.

Contact us about your project and we will reply with a quote along with detailed information regarding your custom Modification Specific Polyclonal Antibody Production!

Purity available Crude Desalt >70% >75% >80% >85% >90% >95% >98%
Quantity available 3 mg 5 mg 10 mg 15 mg 20 mg 25 mg 30 mg 40 mg 50 mg 60 mg 100mg 1000 mg

Our skilled and experienced team of chemists can provide custom synthesis of almost any organic compound. We offer different fluorescent dyes and the preparation of intermediates, reference compounds, starting materials, and derivatives of lead compounds. All compounds produced in our facility are made to order in scales ranging from milligram to multi-gram quantities.

All custom synthesis requests that we receive are to determine whether we wish to pursue the project. This process usually takes no longer than 48 hours. 

The customer shall provide us with known literature methods and/or their own existing synthetic route in which all experimental details (reaction times, temperatures, solvents and concentrations, work-up procedures, purification methods, yields, and analytical data) are described. The more information you provide us with, the shorter the turnaround time and the more competitive price of the project will be. 

How to get started

Please contact us (info@stratech.co.uk) with your requests and we will supply you with a detailed quote as soon as possible. This process usually takes between 24 and 48 hours. The quote will include an estimated price as well as the time required to complete the project. All inquires and subsequent projects are handled in the strictest confidence and will be backed up by secrecy agreements if required. 

FAQs

Antibody Questions

1. How should I store my tube of antibodies?

Please store the antibodies in the original tube at -20°C and do not aliquot. The antibodies are provided in a buffer that contains 50% glycerol, avoid repeated freeze.

2. Can I incubate this antibody for 1 hour at room temperature instead of overnight at 4°C?

The antibody modifications include phosphorylation, acetylation and enzymatic cleavage. Most antibodies raised against such modified sites have a lower affinity when compared with antibodies recognizing whole proteins. So it is highly suggested to prolong antibody and antigen interaction time to obtain best signal to noise ratios by incubating overnight at 4°C.

3. Why isn't this antibody working by Western blot?

A: There are several details of Western blotting procedure that are critical to avoid loss of signal and or high background.
a) Do not over wash the membrane. Washing should be performed with TBS/0.1%Tween-20 three times for 5 minutes each. Excessive washing can wash away the primary antibody.
b) Do not over block. Excess blocking can reduce the specific signal visible with some antibodies. Check datasheet for the recommended blocking time for each antibody.

4. How do you ensure the quality of your antibodies?

Affinity Biosciences strives to test all our products on major applications such as Western Blotting, IP, IHC and ELISA. We constantly test our antibodies on different species including human, mouse and rat and new applications. Product quality is the most important consideration, if you should be interested in a particular application not listed on the datasheet, please contact us at technical@stratech.co.uk for update or for help with any technical questions you may have.

5. How do you control quality for different lots?

Affinity Biosciences strives to achieve product consistency or improvement from lot to lot. As a standard, we test the new lot in all the applications we recommend side by side with the old lot. The recommended dilution for each application is optimized for each lot following our protocols. Concentrations between lots of antibodies are constantly adjusted to obtain better results.

6. Can I order this antibody without BSA or glycerol?

For bulk orders, many of our antibodies can be prepared to the customer’s specifications. Contact us at technical@stratech.co.uk

7. Could you develop an antibody for me?

Gladly! Contact us at technical@stratech.co.uk for details.

8. Will your antibodies work on my whole tissue sample lysates?

We do not test all antibodies on whole tissue lysates in-house, but many of our antibodies have been used by other customers on whole tissues with satisfying results.

Other Technical Questions

1. How should I store my SDS samples of phosphorylated proteins?

SDS sample cell lysates can be stored at -20°C for short-term use (less than 3 months), but should be kept at -80°C for long-term storage. The degradation of activated protein samples varies dramatically, depending on the nature of each protein and how much activated the protein is within the samples.

2. What buffer should I use to prepare my cell lysate samples?

Please check our datasheet for the recommended buffer in the Western blotting protocol.

3. How do I prepare good control cell lysates for this phosphorylated target protein?

Please check our datasheet for the western blot figure in the WB protocol, that will demonstrate a suitable control cell line and drug treatment.

4. If a species is not listed as cross-reactive with your antibody on the datasheet, does this mean that the species has not been tested?

Affinity Biosciences tests all antibodies on human, mouse and rat species if possible. If we do not obtain positive results in one species, we do not list that species. However, it is possible that the cell line we test may contain a low level of particular protein or the inductions we used do not work in that particular cell line. Therefore, we suggest you compare the peptide sequence around the modification site (5 amino acids on each side) between the species you are studying and the species that our antibody was raised against. Generally, our antibodies are raised against the human protein sequence and are highly purified using affinity chromatography. Many times if the sequences are identical or have only 1 or 2 amino acid differences in residues other than the phosphorylation site, there is a good possibility the antibody will recognize your protein.


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