High quality Interferon Gamma products from PBL Assay Science:

• High Specific Activity
• Low in aggregates
• > 98% dimerizedDid you know that Interferon Gamma is only active as a protein dimer? Interferon Gamma are members of the Type II class of interferons. Type II IFN in humans is limited to the single Interferon Gamma gene. This IFN binds to the Type II receptor comprised of IFNGR1 and IFNGR2 chains. Interferon Gamma is produced by cells of the immune system such as B-cells, T-cells and NK cells. Interferon Gamma is glycosylated in mammalian cells and the active form functions as a homodimer. All of PBL’s Interferon Gamma proteins are guaranteed dimers for maximum activity.

Figure 1. Chromatographic profile of IFN-γ showing dimerization and low aggregation

Figure 2. PBL IFN-γ and international gamma standard exhibit comparable antiviral activities

Non-human primates are considered a preferred model for the study of human diseases due to a close phylogenetic relationship to humans. The model is crucial to the elucidation of pathological mechanisms and may enable the development of novel diagnostic and therapeutic tools. Studies of innate and adaptive immunity, pharmacodynamics (PD), and immunotoxicology of interferon stimulating/inhibiting molecules, as well as Emerging Infectious Disease (EID) models, can all be further advanced with an interferon immunoassay that is designed specifically for NHP.

PBL now offers ELISAs for the specific detection of NHP IFN-Alpha and IFN-Beta in a variety of sample matrices. Visit our website or contact us for more information on these kits!

Product Information:

VeriKineTM Cynomolgus/Rhesus Interferon Alpha Serum ELISA (Cat. No. 46100-1)

Compatibility: Serum, Plasma, Cell Culture Supernatant

Assay Range: 25 – 1600 pg/ml

• Quantifies Cynomolgus and Rhesus IFN-α2 in serum, plasma and TCM
• Reproducible results with excellent inter- and intra-assay CVs (<15%)
• Provides greater NHP IFN-α detection sensitivity and matrix compatibility than traditional human IFN-α ELISA kits

Figure 1. Standard Curve in Different Sample Matrices

Figure 2. Specifically designed for detecting NHP IFN-α Greater NHP IFN-α detection sensitivity and matrix compatibility

Product Information:

VeriKineTM Cynomolgus Interferon Beta ELISA (Cat. No. 46415-1)
Compatibility: Serum, Plasma, Cell Culture Supernatant
Assay Range: 5.47 – 350 pg/ml

• Quantifies Cynomolgus IFN-β (Macaca fascicularis) in serum, plasma and TCM
• Reproducible results with excellent inter- and intra-assay CVs (<10%)

Figure 3. Typical standard curve from 5.47 to 350 pg/ml

Figure 4. Performance specifications at three different concentrations

21385-1

Anti-Human Interferon Alpha/Beta Receptor Chain 2 (IFNAR2), Clone MMHAR-2 (MAb)

Figure 1. Mouse MAb to Human IFN α/β Receptor 2 (MMHAR-2)

• Neutralizes biological activity of Human Type I IFNs (α, β, and ω)
• Blocks Type I interferon receptor complex
• Suitable for use in Type I IFN neutralization, flow cytometry, immunohistochemistry, and immunoprecipitation

21885-1

Anti-Human Interferon Lambda Receptor 1, Clone MMHLR-1 (MAb)

Figure 2. Neutralizing MAb against IFNLR1

• Neutralizes human interferon lambda receptor 1

12185-1

C12R Mouse Interferon Alpha Decoy Receptor

Figure 3. C12R Neutralization of Mouse IFN α Subtypes on L929 Cells

• Viral protein C12R neutralizes all 14 mouse IFN α subtypes in vitro
• Neutralizes all human IFN α subtypes

31110-1

Anti-Human Interferon Alpha, Sheep Serum (PAb)