For over 30 years, PBL Assay Science has had a mission to help ‘solve the most difficult problems’ of researchers around the world. From its founding in 1990 and continuing through today, PBL has become widely known as a source for high-quality immunoassay kits, cytokines and interferons, monoclonal and polyclonal antibodies, and other related reagents for life science researchers. PBL products can provide reliable and consistent solutions for all your Assay Science needs.


Product Categories

Categories

Singleplex ELISAs: PBL’s ELISA kits are a comprehensive range of heterogeneous antibody sandwich immunoassays. The detection system utilizes horseradish peroxidase (HRP) and Tetramethyl-benzidine (TMB) as the enzyme-substrate which provides sensitive and robust assays for the detection and quantification of interferons and cytokines.

 

Multiplex ELISAs: Fully quantitative high throughput multiplex ELISA kits that provide simultaneous quantification and detection of up to 16 different analytes from a single sample.

 

Interferons: PBL offers a wide range of interferons from different species and of different classes including multiple IFN-alpha and IFN-beta subtypes. Our interferons are high purity, provide consistent performance and biological activity and are suitable for use in a range of research applications.

 

Cytokines: Cytokines are a class of signaling proteins that play a role in both the induction and effector phases of all immune and inflammatory responses, including cell proliferation, intercellular communication, and cell death. Cytokines are produced by a variety of hematopoietic and non-hematopoietic cell types and can exert autocrine and paracrine effects. They are related more correctly to hormones than to growth factors in their overall functions, but many cytokines also exhibit growth factor activity.

 

Monoclonal and Polyclonal Antibodies: PBL offers a range of both monoclonal and polyclonal antibodies raised against different interferons (IFNs) from various mammalian species. As opposed to other commercially available Anti-IFN antibodies, PBL’s human IFN alpha antibodies are tested against a wide range of human IFN subtypes

Resources

Mouse Interferon Reagents and ELISAs

  • High quality recombinant proteins for research
  • Antibodies validated for biological assay use
  • ELISAs for immunological assay development

As the drug discovery pathway shifts toward specifically targeted pathways and molecules, model systems continue to increase in importance. The ease of use and versatility of mouse models in the study of human cancer, infectious disease and autoimmunity provides understanding into the etiology of these diseases. Data obtained from studies using mouse models can provide insight into potential clinical side effects and the pathology of diseases.

PBL Assay Science provides a comprehensive mouse product line manufactured to the highest standards of quality, consistency and purity. PBL’s products are designed to meet your scientific needs by providing superior quality and performance to ensure reliable and reproducible results.

Figure 1. Comparison of Mu Alpha A and Mu Alpha 4 Antiviral Activity

Figure 2. Recovery of Mu Alpha A in 50% Serum in Mu Alpha ELISA (42120)

Interferons

Product Information

12100-1Mouse IFN Alpha A1 x 105 U
12105-1Mouse IFN Alpha 11 x 105 U
12115-1Mouse IFN Alpha 4, mammalian1 x 105 U
12125-1Mouse IFN Alpha 111 x 105 U
12130-1Mouse IFN Alpha 131 x 105 U
12185-1NEW C12R Mouse IFN Alpha Decoy Receptor, neutralizing10 μg
12185-2NEW C12R Mouse IFN Alpha Decoy Receptor, neutralizing100 μg
12400-1Mouse IFN Beta1 x 105 U
12401-1Mouse IFN Beta (carrier-free)1 x 105 U
12405-1Mouse IFN Beta, mammalian1 x 105 U
12410-1Mouse IFN Beta, mammalian (carrier-free)1 x 106 U
12820-1Mouse Interleukin-28b/IFN Lambda 325 μg
12821-1Mouse Interleukin-28b/IFN Lambda 3 (carrier-free)25 μg
  • Recombinant proteins derived from bacterial or mammalian cells
  • Consistent performance and biological activity
  • > 95% purity
  • Endotoxin level <1 EU/μg
  • Suitable for use in a range of applications such as ELISAs and cytopathic effect assays
  • Viral C12R IFN Antagonist neutralizes all mouse IFN alpha subtypes

Antibodies

22100-1Anti-Mouse IFN Alpha, Clone RMMA-1 (MAb)250 μg
22100-3FITC Conjugated Anti-Mouse IFN Alpha, Clone RMMA-1 (MAb)25 μg
22400-1Anti-Mouse IFN Beta, Clone RMMB-1 (MAb)250 μg
22400-3FITC Conjugated Anti-Mouse IFN Beta, Clone RMMB-1 (MAb)25 μg
22500-1Anti-Mouse IFN Gamma, Clone RMMG-1 (MAb)500 μg
32100-1Anti-Mouse IFN Alpha, Rabbit Serum (PAb)2 x 104 U
32120-1Anti-Mouse IFN Alpha, Chicken IgY, Purified (PAb)500 μg
32400-1Anti-Mouse IFN Beta, Rabbit Serum (PAb)2 x 104 U
32401-1Anti-Mouse IFN Beta, Rabbit IgG, Protein A Purified (PAb)2 x 104 U
32500-1Anti-Mouse IFN Gamma, Rabbit Serum (PAb)2 x 104 U
  • High specificity
  • Monoclonal antibodies have > 95% purity
  • Suitable for use in a range of applications such as ELISAs, neutralization, flow cytometry, and western blotting

ELISAs

42120-1VeriKine Mouse IFN Alpha ELISA Kit1 plate
42120-2VeriKine Mouse IFN Alpha ELISA Kit5 plate
42115-1NEW VeriKine-HS Mouse IFN Alpha All-Subtype ELISA Kit1 plate
42400-1VeriKine Mouse IFN Beta ELISA Kit1 plate
42400-2VeriKine Mouse IFN Beta ELISA Kit5 plate
42410-1VeriKine-HS Mouse IFN Beta Serum ELISA Kit1 plate
42410-2VeriKine-HS Mouse IFN Beta Serum ELISA Kit5 plate
52500-1NEW VeriPlex Mouse Cytokine 9-Plex ELISA Kit1 plate
62830-1DIY Mouse IFN Lambda 2/3 (IL-28A/B) ELISAMaterials for 15 x 96 wells
  • Mouse Alpha ELISAs provide global interferon alpha measurement in serum, plasma, and tissue culture media
  • < 10% inter- and intra- assay CVs
  • High sensitivity ELISA options provide accurate results ~2 hours
  • NEW Multiplex ELISA

Quality and Value - A Perfect Dimer!

High quality Interferon Gamma products from PBL Assay Science:

  • High Specific Activity
  • Low in aggregates
  • > 98% dimerizedDid you know that Interferon Gamma is only active as a protein dimer? Interferon Gamma are members of the Type II class of interferons. Type II IFN in humans is limited to the single Interferon Gamma gene. This IFN binds to the Type II receptor comprised of IFNGR1 and IFNGR2 chains. Interferon Gamma is produced by cells of the immune system such as B-cells, T-cells and NK cells. Interferon Gamma is glycosylated in mammalian cells and the active form functions as a homodimer. All of PBL’s Interferon Gamma proteins are guaranteed dimers for maximum activity.

Figure 1. Chromatographic profile of IFN-γ showing dimerization and low aggregation

Figure 2. PBL IFN-γ and international gamma standard exhibit comparable antiviral activities

Product Information

Interferon Gamma Products

11500-1Human IFN Gamma50 μg
11500-2Human IFN Gamma100 μg
13500-1Rat IFN Gamma10 μg
21500-1Anti-Human IFN Gamma, Clone MMHG-1 (MAb)500 μg
21550-1Anti-Human IFN Gamma, Clone MMHG-2 (MAb)100 μg
21585-1Anti-Human IFN Gamma Receptor Chain 2, Clone MMHGR-2 (MAb)250 μg
22500-1Anti-Mouse IFN Gamma, Clone RMMG-1 (MAb)500 μg
31500-1Anti-Human IFN Gamma, Rabbit Serum (PAb)2 x 104 U
32500-1Anti-Mouse IFN Gamma, Rabbit Serum (PAb)2 x 104 U
41500-1VeriKine Human IFN Gamma ELISA Kit1 plate
41580-1NEW VeriKine Human IFN Gamma Receptor 1 ELISA Kit1 plate

Sensitive and Specific Non-Human Primate (NHP) ELISAs for serum, plasma and tissue culture media matrices

Non-human primates are considered a preferred model for the study of human diseases due to a close phylogenetic relationship to humans. The model is crucial to the elucidation of pathological mechanisms and may enable the development of novel diagnostic and therapeutic tools. Studies of innate and adaptive immunity, pharmacodynamics (PD), and immunotoxicology of interferon stimulating/inhibiting molecules, as well as Emerging Infectious Disease (EID) models, can all be further advanced with an interferon immunoassay that is designed specifically for NHP.

PBL now offers ELISAs for the specific detection of NHP IFN-Alpha and IFN-Beta in a variety of sample matrices. Visit our website or contact us for more information on these kits!

Product Information:

VeriKineTM Cynomolgus/Rhesus Interferon Alpha Serum ELISA (Cat. No. 46100-1)

Compatibility: Serum, Plasma, Cell Culture Supernatant

Assay Range: 25 – 1600 pg/ml

 

  • Quantifies Cynomolgus and Rhesus IFN-α2 in serum, plasma and TCM
  • Reproducible results with excellent inter- and intra-assay CVs (<15%)
  • Provides greater NHP IFN-α detection sensitivity and matrix compatibility than traditional human IFN-α ELISA kits

Figure 1. Standard Curve in Different Sample Matrices

Figure 2. Specifically designed for detecting NHP IFN-α Greater NHP IFN-α detection sensitivity and matrix compatibility

Product Information:

VeriKineTM Cynomolgus Interferon Beta ELISA (Cat. No. 46415-1)
Compatibility: Serum, Plasma, Cell Culture Supernatant
Assay Range: 5.47 – 350 pg/ml

  • Quantifies Cynomolgus IFN-β (Macaca fascicularis) in serum, plasma and TCM
  • Reproducible results with excellent inter- and intra-assay CVs (<10%)

Figure 3. Typical standard curve from 5.47 to 350 pg/ml

Figure 4. Performance specifications at three different concentrations

21385-1

Anti-Human Interferon Alpha/Beta Receptor Chain 2 (IFNAR2), Clone MMHAR-2 (MAb)

Figure 1. Mouse MAb to Human IFN α/β Receptor 2 (MMHAR-2)

  • Neutralizes biological activity of Human Type I IFNs (α, β, and ω)
  • Blocks Type I interferon receptor complex
  • Suitable for use in Type I IFN neutralization, flow cytometry, immunohistochemistry, and immunoprecipitation

21885-1

Anti-Human Interferon Lambda Receptor 1, Clone MMHLR-1 (MAb)

Figure 2. Neutralizing MAb against IFNLR1

  • Neutralizes human interferon lambda receptor 1
Sample EC50 (ng/ml)
10 ng/ml L1 31.3
10 ng/ml L2 54.3
3 ng/ml L3 22.0

12185-1

C12R Mouse Interferon Alpha Decoy Receptor

Figure 3. C12R Neutralization of Mouse IFN α Subtypes on L929 Cells

  • Viral protein C12R neutralizes all 14 mouse IFN α subtypes in vitro
  • Neutralizes all human IFN α subtypes

31110-1

Anti-Human Interferon Alpha, Sheep Serum (PAb)

Figure 4. Neutralization of Human IFN α subtypes on A549 cells

  • Neutralizes all human interferon alpha subtypes
  • Suitable for use in neutralization and Direct Binding ELISA

FAQs

Interferons and Cytokines

What is the level of endotoxin of your proteins?

< 1 EU/μg

What is the difference between formulation with carrier protein and formulation with carrier-free?

Carrier protein (PBS with 0.1% BSA) was added to provide extra stability to the cytokine proteins. However, in certain applications, such as in vivo injection, conjugation, or surface binding, carrier protein may interfere.

How to convert interferon specific activity to mass?

The formula to use to convert S.A. to pg/ml is as follow: [(1 x 10E+09)/(Lot specific activity in units/mg)] x [Lot-specific concentration in units/ml]

For interferon beta (Catalog No. 11410), what should I use for dilution?

Culture medium supplemented with serum. Interferon beta proteins are unstable at diluted concentrations. Unless formulation work has been done to verify stability, do not dilute carrier-free solution to protein concentration below 100 μg/ml.

Why is human interferon beta (Catolog No. 11410) shipped in acetic acid?

Acetic acid prevents aggregation of interferon beta proteins in this particular product formulation.

Antibodies

Does PBL offer controls for its antibodies?

PBL does not offer any controls for its antibodies at the current time.

What control should I use?

For monoclonal antibodies, use normal antibody from the same species and the same isotype. For example, if a MAb is a murine IgG2A, use the normal equivalent as the negative control. Some polyclonal antibodies from PBL are unpurified sera. Therefore, investigators should use normal unpurified serum from the appropriate species as their negative control.

How much neutralizing antibody should I use?

User cell lines vary so there is no specific answer to this question. However, the general rule is 70 fold excess in mass should be used for neutralizing interferon. It is recommended to quantify the amount of interferon via technique such as ELISA. It is also recommended to allow time (1-2 hours in general) for the antibody to neutralize the interferon before adding the virus.

How are antibodies determined to be neutralizing?

he viral challenge assay is used to test antibodies for neutralization activity. The assay measures the ability of the antibody to negate the protective effects of the interferon against the protective effects of the interferon against the viral challenge.

Assay Kits

What types of antibodies are used in PBL ELISA kits?

All PBL ELISA kits are constructed with carefully selected proprietary antibodies and optimized reagents.

Can I use serum or plasma in your assay kits?

PBL offers a range of assay kits that are specifically designed for serum/plasma human interferon alpha (41110, 41115), human interferon beta (41415), mouse interferon beta (42410, 42400), cynomolgus interferon alpha (46100), human IFNs and cytokines (51500).

Does PBL offer ELISA reagents separately?

Due to lot-specific optimization of our ELISA kit reagents, they are not sold separately.

What are the specifications for optimization of the reagents for each lot of the kits?

To optimize each lot of kits, PBL modifies the concentration of the reagents to achieve a standard curve that will meet our specific range and signal to noise requirements.

Can components from different lots be used as substitutes?

No. Reagent concentration is optimized according to lot. Reagents from different lot can not be used as substitutes without affecting the overall performance of the kit.


PBL Assay Science - Interferon Information

General Interferon

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Universal IFN- alpha Antibody and ELISA

Human IFN-alpha detection or neutralization of all subtypes

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First for Antibodies

Stratech Scientific have been successful in a recent antibodies tender from the Southern Universities Purchasing Consortium. In the award criterion Read More…

Frequently Asked Questions

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VeriPlex Multiplex ELISAs

PBL Assay Science’s VeriPlex ELISAs are fully quantitative, high throughput multiplex ELISA kits that provide simultaneous quantification and detection of Read More…