Founded in 2007 in Beijing P.R.C by MIT Professor Dr Daniel I. C. Wang, Sino Biological focus on the manufacture of proteins, antibodies, transfection reagents and enzymes and their goal is to produce in bulk proteins for every known human gene with a potential research application.

Sino have extensive expertise in many fields and their customers include global pharmaceutical and Biotech companies and academic institutions worldwide.


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Featured Research Reagents

Recombinant Proteins

Immune Checkpoint Proteins

Research and Develop Proteins for Almost 50 Immune Checkpoints Targets
Covering Multiple Species and Various Tags
Binding Activity is Assayed and Confirmed

Drug Target Proteins

Covering Drug Development Target, CAR-T Target, Fc Receptors, Immune Checkpoint, etc.
Binding Activity to MAb Drugs Validated; Cell-based Activity Assay
Multiple Species, Various Tags

Biotinylated Proteins

Strict Quality Control to Guarantee Activity on Every Recombinant Biotinylated Protein
Cover Almost All MAb Drug Target Proteins: B7/CD28, PCSK9, TNFSFs…
Biotinylated CAR-T Relevant Proteins & Fc Receptor Proteins

Fc Receptor Proteins

Biotinylated Fc Receptor Protein Available
High Purity: >95%; Low Endotoxin: <1.0 EU/μg
Multiple Species: Human, Mouse, Rat, Monkey

Influenza Virus Proteins

More than 60 Subtypes: H1-H18, N1-N11, Influenza B
More than 250 Strains: Vaccine Strains, HPAI Strains, High Attention Strains, New Strains
HA: High Hemagglutination Activity; NA: High Eenzymatic Activity

Cytokine Proteins

Covering All Cytokine Families (900 Cytokines & Receptors), Multiple Species, Various Tags
GMP Level Cytokines Available; Apply to Cell Culture,
Animal Free, Carrier Free, High Purity; Better Bioactivity

Elite Antibodies / ELISA Kits

Elite Rabbit Mab (EliteRmab®)

80% Using Eukaryotic Recombinant Proteins as Immunogen
More Sensitive and Specific, Better for Small Molecule
10-100× Higher Affinity than Mouse Antibodies

IHC Antibody

Examinations by Multiple Tissues;Specificity Ensured by Antigen Blocking
Excellent Reproducibility, High Sensitivity
Strict Quality Assurance System

Features for His Tag Antibody

High Sensitivity: Detects Lower Expression Levels
Different Positions: Recognizes His Tags at N-terminal, C-terminal or Internal
Broad Spectrum: Identify More His-tagged Proteins

ELISA Kits

8 International QC Test Indicators
ELISA Kit for Human Cytokines: IL-4,IL-6,IL-10,IL-12,IL-17A,GM-CSF,TNFα,TNFβ, CXCL9…

Genes and Others

cDNA with GFPSpark & OFPSpark Tag

Bright Fluorescence, High pH-stability
Full-length Sequence Confirmed, Expression Validated
Recommended for Gene Expression Analysis, Cell Labeling and Organelle Labeling

Sinofection® Transfection

Higher Transfection Efficiency
Inorganic Reagent without Animal Ingredients
Higher Protein Expression, Lower Cytotoxicity
For DNA Transfection, Eukaryotic Cells Like HEK293
For Both Adherent and Suspension Cells

Protease

Be Used in More than 1000 Protein’s Purification, High Protease Activity
Suitable for Recombinant Protein’s Purification

Nuclease

Be Used in More than 2000 Proteins’ Purification
Without Tag, Easy to be Removed
Suitable for Vaccine’s Purification, Recombinant Antibody’s Purification
Suitable for Sample Preparation of WB and The Like

Antigen and Antibody Production Services

Sino Biological offers comprehensive recombinant expression services to clients in the biomedical industry. The technical team stands behind years of experience in producing high-quality protein and antibody products from a variety of platforms, including mammalian cells, insect cells, and E. Coli. Over the years, Sino Biological has established business relationships with many companies and research institutions around the globe, ranging from start-up labs to the world’s top ten pharmaceutical companies.

Recombinant Antibody Production Service Types

High-throughput Antibody Production Service (0.1mg-1mg)

Timeline: 2-4 weeks

• Gene synthesis and codon optimization (Optional)
• Vector construction
• Expression & purification
• QC analysis

Large-scale Antibody Production Service (100mg-100g)

Timeline: 4-6 weeks

• Gene synthesis and codon optimization (Optional)
• Vector construction
• Expression & purification
• QC analysis

Chimeric Antibody Production Service

• Gene synthesis and codon optimization (Optional)
• Vector construction
• Expression & purification
• QC analysis

Antibody Fragment Production Service

• Gene synthesis and codon optimization (Optional)
• Vector construction
• Expression & purification
• QC analysis

Bispecific Antibody Production Service

• Gene synthesis and codon optimization (Optional)
• Vector construction
• Expression & purification
• QC analysis

Cases Studies of Recombinant Antibody Production Service

Sino Biological Inc. can achieve rapid and high-throughput recombinant antibody production. The preparation cycle is only 2-6 weeks, and the monomer purity can reach 99%

Recombinant Antibody Production Service Highlights

Capacity and technology platform

• >20 shakers, >800 bottles culture capacity weekly
• >80 bioreactors (2L~1000L)
• Cell culture capacity: >50,000 L yearly
• Free codon optimization
• Optimized high-efficiency expression vectors
• Optimized proprietary culture medium
• Optimized mammalian cell expression platform

Rich experience

• Experienced in expressing different kinds of antibodies:
scFv antibody, Fab antibody, full length antibody, chimeric antibody, bispecific antibody, multi-chains co-transfection and expression, etc.
• Experienced in expressing and purifying multi-isotypes antibodies:
Human IgG1, IgG2, IgG3, IgG4
Mouse IgG1, IgG2a, IgG2b, IgG3
Rat IgG1, IgG2a, IgG2b, IgG2c
Rabbit IgG
Canine IgG
IgA, IgM, IgE

High quality

• High monomer purity: up to 99%
• Low endotoxin level: as low as 0.1 EU/mg
• Comprehensive quality control analysis:
UV, SDS-PAGE, ELISA, qPCR, SEC-HPLC, CEX-HPLC, LC-MS/MS, CE-SDS, ADCC,CDC, OCTET Affinity, Kinetics, off-rate, ranking

Why choose Sino Biological for your protein production service?

• Experienced in the successful development and production of about 6000 recombinant proteins.
• One-stop service from gene to protein
• Multiple protein expression systems available including bacteria, yeast, baculovirus-insect and mammalian expression systems
• Multiple purification systems (30+) from which to choose
• Comprehensive research and development capabilities with over 1000 new proteins generated per year
• 80+ bioreactors with various volume capacities ranging from 2 to 1000 L to meet high-throughput and large-scale production requirements

Overview of Recombinant Protein Expression: Key Influencing Factors, Case Studies and FAQs

E.coli protein expression services

• Gene synthesis and codon optimization (optional)
• Vector construction
• Expression evaluation
• Protein expression and purification
• Large-scale expression and purification (optional)

Mammalian transient protein expression services

• Gene synthesis and codon optimization (optional)
• Vector construction
• Expression and purification
• Large-scale expression and purification (optional)

Baculovirus-insect expression services

• Gene synthesis and codon optimization (optional)
• Vector construction
• Virus packaging and amplification
• Expression and purification
• Large-scale expression and purification (optional)

Case study of E.coli protein A expression

SEC-HPLC >98.5%

Cell bioactivity: EC50=17pg/mL

Case study of Baculovirus-Insect protein A expression

SEC-HPLC >93%

Cell bioactivity: EC50=7ng/mL

Case study of HEK293 transient protein A expression

SEC-HPLC >98.2%

SDS-PAGE >98.7%

Custom Recombinant Protein Production Service Advantages

Mammalian Transient Protein Expression Service

• High-efficiency expression vectors
• Optimized mammalian expression systems
• High-throughput and large-scale production
• High yield expression with post-translational modification and correct folding
• One-stop service from gene sequence to purified protein

E.coli Protein Expression Service

• Years of experience of protein expression
• Multiple protein purification and refolding technologies
• High-throughput and large-scale protein expression and production
• One-stop service from gene to purified protein
• Multiple expression vectors and E.coli cells to meet various expression needs

Baculovirus-Insect Cell Protein Expression Service

• High-efficiency expression of intracellular and extracellular proteins
• Multiple insect cells: Sf9,Sf21,Hi-5
• High-throughput and large-scale protein production
• Optimized expression vectors and higher-titre virus packaging

Recombinant Protein Expression Systems

Bacterial, yeast, insect and mammalian protein expression systems are widely used. Each system has its advantages and challenges. For successful recombinant protein expression, it is important to choose the right system for a specific application. Protein type, function and desired protein yield will also influence the choice of the system.

Expression Systems E.coli Yeast Baculovirus-insect Mammalian
Prokaryotic Eukaryotic Eukaryotic Eukaryotic
Advantages • Low cost
• Rapid expression
• Easy scale-up
• Most widely used system for recombinant protein production
• Low cost
• Rapid expression
• High yield
• Diverse post-translational modifications
• High capacity genes
• Soluble proteins
• Suitable for toxic proteins
• Post-translational modifications similar to mammalian systems
• Soluble protein
• Lower endotoxin
• Better bioactivity
• Comprehensive post-translational modifications
• Transient & stable expression
Challenges • Inclusion bodies
• Lack of eukaryotic post-translational modifications
• Difficult to express higher MW proteins
• Improper glycosylation
• High mannose modification
• More demanding culture conditions
• Lack of partial glycosylation
• More demanding culture conditions
Most Common Applications • Bacterial proteins
• Antigen proteins
• Cytokines
• Enzymes
• Cytokines
• Lower MW proteins
• Enzymes
• Cytoplasmic proteins
• Toxic proteins
• Transmembrane proteins
• Secretory proteins
• Kinases
• Secretory proteins
• Extracellular domain of transmembrane proteins
• Recombinant antibodies
• antibody fragments

Track Records

  • >400 recombinant monoclonal antibody production projects (10mg to 30 gram) for our customers worldwide in the past two years, More than 100 of these are intended for pre-clinical in vivo studies
  • >600 recombinant proteins produced at bulk quantities in two years
  • Produced H1N1 hemagglutinin protein from the 2009 swine influenza within 30-days of public release of the virus gene sequence
  • First in developing high affinity and highly specific monoclonal antibodies to the 2009 H1N1 virus (no cross-reactivity to seasonal flu H1N1 viruses). Our ELISA kit can detect pg/All H1N1 HA protein.

Capabilities and Capacities

  • Extensive expertise including molecular biology, protein and antibody production, rabbit and mouse monoclonal antibody generation, biological QC testing, biological formulation development and stability testing, and diagnostic assay development.
  • >50 stirred tank bioreactors for cell culture and fermentation and purification systems.
  • >30,000 L cell culture per year.
  • >1000 recombinant monoclonal antibody production by transient transfection per year.
  • >500 recombinant protein bulk production per year.
  • >1000 rabbit and mouse monoclonal antibody development programs per year.
  • No lead time for customer projects.

Reliable, Speedy, High Quality, Cost-effective One-Stop Biology Services

  • Demonstrated to be the most reliable partner with close to 100% success rate.
  • Unmatched speed of protein and antibody production is in the industry.
  • Our quality of services have won praises from top pharmaceutical and biotech customers
  • 40-80% of cost saving for services.

Business Focus

  • One-Stop Biology Services for protein and antibody discovery, research, development, production, and commercialization
  • Affordable Premium Quality Reagents: Proteins, Antibodies, Kits, Transfection Reagents, Enzymes, Resins, and other Research Tools

Advanced Technology Platforms

  • HEK293 Transient Transfection Platform for Recombinant Protein & Antibody Production
  • CHO Transient Transfection Platform for Recombinant Protein & Antibody Production
  • CHO Stable Cell Line Platform for Recombinant Protein & Antibody Production
  • Bacterial (E. Coli.) Platform for Recombinant Protein Expression & Production
  • Baculovirus Insect Cell Platform for Recombinant Protein Expression & Production
  • Protein and Antibody Purification Platform
  • Rabbit Monoclonal Antibody Development Platform
  • Mouse Monoclonal Antibody Development Platform

Affordable Premium Quality Reagents

They intend to produce bulk quantity of human proteins for every known human gene as long as it has potential research application, and develop high affinity antibodies for all proteins They produce. Our premium quality reagents are provided at significant cost-saving to our customers worldwide.

  • High purity recombinant proteins, majority produced in mammalian cells
  • High affinity antibodies: both rabbit and mouse monoclonal antibodies
  • Majority antibodies developed with protein-based antigen
  • Highly sensitive and specific ELISA kits

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Virus Research

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SARS-CoV-2/COVID-19

Covid-19 Webinar Registration

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