Compounds | Code | Name |
List of compounds | L1050 | DiscoveryProbe™ Anti-Virus Compound Library |
List of compounds | L1021 | DiscoveryProbe™ FDA-approved Drug Library |
Compounds | Code | Name |
List of compounds | L1050 | DiscoveryProbe™ Anti-Virus Compound Library |
List of compounds | L1021 | DiscoveryProbe™ FDA-approved Drug Library |
Cat No. | Product Name | Information |
B4874 | Hydroxychloroquine Sulfate | Hydroxychloroquine Sulfate is an antimalarial agent used for the treatment of systemic lupus erythematosus, rheumatoid arthritis and other autoimmune, inflammatory and dermatologic conditions. Also acts as an inhibitor of autophagy and toll-like receptor (TLR) 7/9. |
A1933 | Carfilzomib (PR-171) | Carfilzomib (PR-171) is an irreversible proteasome inhibitor with IC50 of <5 nM in ANBL-6 cells, displayed preferential in vitro inhibitory potency against the ChT-L activity in the β5 subunit, but little or no effect on the PGPH and T-L activities. |
B1398 | Azithromycin | Azithromycin is an antibiotic by inhibiting protein synthesis, used for the treatment of bacterial infections. |
B4950 | Darunavir Ethanolate | Darunavir Ethanolate (DRV) is a nonpeptidic HIV protease inhibitor, used to treat HIV infection. |
B3477 | Ciclesonide | Ciclesonide is a glucocorticoid used to treat obstructive airway diseases. |
B2285 | Daunorubicin HCl | Daunorubicin HCl inhibits both DNA and RNA synthesis and inhibits DNA synthesis with Ki of 0.02 μM in a cell-free assay. |
A8628 | Chloroquine diphosphate | Chloroquine diphosphate is a 4-aminoquinoline anti-malarial and anti-rheumatoid agent, also acting as an ATM activator. |
B1539 | Tideglusib | Tideglusib is an irreversible, non ATP-competitive GSK-3β inhibitor with IC50 of 60 nM in a cell-free assay; fails to inhibit kinases with a Cys homologous to Cys-199 located in the active site. Phase 2. |
B2082 | Camostat Mesilate | Camostat is a trypsin-like protease inhibitor, inhibits airway epithelial sodium channel (ENaC) function with IC50 of 50 nM, less potent to trpsin, prostasin and matriptase. |
B8398 | Remdesivir (GS-5734) | Remdesivir,a monophosphoramidate prodrug of an adenosine analog, is an investigational broad-spectrum antiviral agent with in vitro activity against multiple RNA viruses, including Ebola and CoV. |
A8504 | Pitavastatin Calcium | Pitavastatin calcium, a novel member of the medication class of statins, is a calcium salt formulation of pitavastatin which is a highly effective HMG-CoA reductase inhibitor. |
A4015 | Disulfiram | Disulfiram is a specific inhibitor of aldehyde-dehydrogenase (ALDH1), used for the treatment of chronic alcoholism by producing an acute sensitivity to alcohol. |
A2586 | Nafamostat Mesylate | Nafamostat mesilate is a synthetic serine protease inhibitor, used as an anticoagulant during hemodialysis. |
A8458 | Lamivudine | Lamivudine is a potent nucleoside analog reverse transcriptase inhibitor, used for treatment of chronic HBV and HIV/AIDS. It works by blocking the HIV reverse transcriptase and hepatitis B virus polymerase. |
N2643 | Shikonin | Shikonin, a potent and specific Pyruvate kinase M2 (PKM2) inhibitor, is a major component of zicao (purple gromwell, the dried root of Lithospermum erythrorhizon), a Chinese herbal medicine with various biological activities. It is also an inhibitor of TMEM16A chloride channel activity using cell-based fluorescent-quenching assay. |
A8204 | Lopinavir | Lopinavir is a potent HIV protease inhibitor with Ki of 1.3 pM in a cell-free assay. |
A3653 | Nelfinavir Mesylate | Nelfinavir Mesylate is a potent HIV protease inhibitor with Ki of 2 nM. |
A8203 | Ritonavir | Ritonavir is a Cytochrome P450 3A and Protease Inhibitor; Also inhibits Cytochrome P450 2D6, P-Glycoprotein and induces Cytochrome P450 2C19, Cytochrome P450 1A2, Cytochrome P450 2C9, Cytochrome P450 2B6 and UDP Glucuronosyltransferases. |
A2813 | Ivermectin | Ivermectin is a glutamate-gated chloride channel (GluCls) activator, used as a broad-spectrum antiparasitic drug. |
A5275 | Tenofovir | Tenofovir blocks reverse transcriptase and hepatitis B virus infections. |
B1123 | Rosuvastatin | Rosuvastatin is an inhibitor of HMG-CoA reductase, an enzyme that catalyzes the rate-limiting step in cholesterol biosynthesis, with Ki value (inhibition constant) of approximately 0.1 nM. |
A8206 | Darunavir | Darunavir is a nonpeptidic HIV protease inhibitor, used to treat HIV infection. |
A4031 | Telaprevir (VX-950) | Telaprevir (VX-950) is an HCV NS3-4A serine protease inhibitor with IC50 of 0.35 μM. |
A8518 | Rosuvastatin Calcium | Rosuvastatin Calcium is a competitive inhibitor of HMG-CoA reductase with IC50 of 11 nM in a cell-free assay. |
A2548 | Carmofur | Carmofur is a highly potent acid ceramidase inhibitor, used in the treatment of breast and colorectal cancer. |
A4509 | PX-12 | PX-12 is a potent thioredoxin-1 (Trx-1) inhibitor by irreversibly thioalkylation of Cys73 of Trx-1. Phase 2. |
B2078 | Atovaquone | Atovaquone is a medication used to treat or prevent for pneumocystis pneumonia, toxoplasmosis, malaria, and babesia. |
B1249 | TDZD-8 | TDZD-8 is a non-ATP competitive GSK-3β inhibitor with IC50 of 2 μM; minimal inhibitory effect observed on CDK1, casein kinase II, PKA and PKC. |
B2011 | Praziquantel | Praziquantel is an anthelmintic effective against flatworms. |
A3261 | Boceprevir | Boceprevir is an oral, direct acting hepatitis C virus (HCV) protease inhibitor with Ki value of 14 nM for NS3. It is used in combination with other antiviral agents in the treatment of chronic hepatitis C, genotype 1. |
B2212 | Moexipril HCl | Moexipril HCl is a potent orally active nonsulfhydryl angiotensin converting enzyme (ACE) inhibitor, used for the treatment of hypertension and congestive heart failure. |
B1569 | Bepotastine Besilate | Bepotastine is a non-sedating, selective antagonist of histamine 1 (H1) receptor with pIC50 of 5.7. |
C4696 | Ebselen | Ebselen is a small-molecule capsid inhibitor of HIV-1 Replication with IC50 of 46.1 nM in TR-FRET assay. |
Coronaviruses are named for the crown-like spikes on their surface. There are four main sub-groupings of coronaviruses, known as alpha, beta, gamma, and delta. Novel coronavirus (SARS-CoV-2) is a beta coronavirus. SARS-CoV-2 is a positive-sense, single-stranded RNA coronavirus that causes COVID-19, the infectious disease that can cause an acute respiratory infection. Studies show that the SARS-CoV-2 S protein retains sufficient affinity to the cellular Angiotensin converting enzyme 2 (ACE2) protein, and likely uses ACE2 protein as a receptor for cellular entry.
There is currently no specific medicine or treatment for diseases caused by SARS-CoV-2. We provide reverse transcription and qPCR series to help efficiently detect SARS-CoV-2, and also provide mRNA in vitro synthesis platform for mRNA vaccines development.
1. Reverse Transcriptase, qPCR Series
qPCR amplification curve of SARS-CoV-2 S gene. The initial number of copies of template DNA: 10/20/200 copies. Viral RNA was extracted from samples derived from patients with SARS-CoV-2. Reverse transcription was performed using reverse transcriptase (Cat. No. K1071) to obtain cDNA products. The cDNA was then quantified using 2X SYBR Green qPCR Master Mix (Cat. No. K1072)
Cat.No. | Product Name | Information |
K1070 | 2×SYBR Green qPCR Master Mix | 2X PreMix for quantifying target DNA or cDNA |
K1071 | Reverse Transcriptase | Thermally stable reverse transcriptase used to synthesize complementary DNA (cDNA) from an RNA template |
K1072 | First-Strand cDNA Synthesis Kit | Synthesize first-strand cDNA from purified poly(A)+ or total RNA |
K1046 | RNase Inhibitor, Murine | RNase Inhibitor, Murine specifically inhibits RNases A, B and C to protect RNA from degradation |
2. RNA In Vitro Synthesis Platform
VLPs were visualized under an electron microscope. We observed particles with striking features of coronavirus. The outline of the envelope for most particles was clear, the spikes were visible. The average size of the particle is 70nm in diameter for the membrane envelope and 90nm when including the spikes, consistent with reported native 2019-nCoV virus. (Ref.1)
The trimeric structure of the extra-vesicular domain of the spike protein from SARS-CoV. The receptor-binding domain (RBD) was used as the antigen. Use mRNA to express the receptor-binding domain of the spike protein (S-RBD). The S glycoprotein is responsible for binding to the receptor through its RBD, enabling the virus to enter into target cells by fusing with cell membranes. The western blot analysis was used to confirm the expression of target proteins. (Ref.1)
Reference:
1.Towards an effective mRNA vaccine against 2019-nCoV: demonstration of virus-like particles expressed from an modified mRNA cocktail.
Affordable Cost, High Yields
APExBIO offers affordable custom synthesis of mRNA and long RNA (up to multiple kilobases) with a wide array of modification services at scales ranging from micrograms to milligrams. The mRNA can be generated from DNA templates provided by our customers or we can provide a full service from the ground up. We provide mCAP, ARCA and EZ Cap (equal to CleanCap) capping or modified nucleotides implication for all our standard mRNA transcripts.
For ordering and more information, please contact sales@stratech.co.uk. Our support team will review and provide a quotation soon.
Cat.No. | Product Name | Cat.No. | Product Name |
B8175 | ARCA | B8174 | mCAP |
B7972 | Pseudo-UTP | B7967 | 5-Methyl-CTP |
B8061 | 5-Methoxy-UTP | K1046 | RNase Inhibitor, Murine |
K1047 | HyperScribe? T7 High Yield RNA Synthesis Kit | K1053 | HyperScribe? Poly (A) Tailing Kit |
K1060 | HyperScribe? T7 High Yield Fluorescein RNA Labeling Kit | K1061 | HyperScribe? T7 High Yield Cy3 RNA Labeling Kit |
K1062 | HyperScribe? T7 High Yield Cy5 RNA Labeling Kit | K1063 | HyperScribe? All in One mRNA Synthesis Kit (ARCA, T7, poly(A)) |
K1064 | HyperScribe? All in One mRNA Synthesis Kit Plus 1 (ARCA, 5mCTP, ψUTP, T7, poly(A)) | K1065 | HyperScribe? All in One mRNA Synthesis Kit Plus 2 (ARCA, 5-moUTP, T7, poly(A)) |
K1066 | HyperScribe? All in One mRNA Synthesis Kit II (EZ Cap Reagent AG (3’ OMe), T7, poly(A)) | K1067 | HyperScribe? All in One mRNA Synthesis Kit II Plus 1 (EZ Cap Reagent AG (3’ OMe), 5mCTP, ψUTP, T7, poly(A)) |
K1068 | HyperScribe? All in One mRNA Synthesis Kit II Plus 2 (EZ Cap Reagent AG (3’ OMe), 5-moUTP, T7, poly(A)) |
Cat.No. | Product Name | Cat.No. | Product Name |
R1001 | ARCA EGFP mRNA | R1002 | ARCA EGFP mRNA (5mCTP, ψUTP) |
R1003 | mCAP EGFP mRNA | R1004 | mCAP EGFP mRNA (5mCTP, ψUTP) |
R1005 | Firefly Luciferase mRNA (ARCA, 5mCTP, ψUTP) | R1006 | SpCas9 mRNA (ARCA, 5mCTP, ψUTP) |
R1007 | ARCA EGFP mRNA (5-moUTP) | R1008 | ARCA Cy3 EGFP mRNA (5-moUTP) |
R1009 | ARCA Cy5 EGFP mRNA (5-moUTP) | R1010 | EZ Cap? Cy5 Firefly Luciferase mRNA (5-moUTP) |
R1011 | EZ Cap? Cy5 EGFP mRNA (5-moUTP) | R1012 | Firefly Luciferase mRNA (ARCA, 5-moUTP) |
R1013 | EZ Cap? Firefly Luciferase mRNA (5-moUTP) | R1014 | ARCA Cas9 5-moUTP |
R1015 | EZ Cap? Cas9 5-moUTP | R1016 | EZ Cap? EGFP mRNA (5-moUTP) |
R1017 | EZ Cap? mCherry mRNA (5mCTP, ψUTP) | R1018 | EZ Cap? Firefly Luciferase mRNA |
B8178 | EZ Cap? Reagent AG (3’ OMe) |
Antibody Drugs and Vaccine Development Related Products
Cat.No. | Product Name | Information |
B8293 | ddhCTP | Chain terminator for the RNA-dependent RNA polymerases. Novel antiviral nucleotide molecules. |
B8362 | 2,3-cGAMP | Immune adjuvant, can specifically activate STING pathway, stimulate innate immunity and accelerate antibody production. |
BC1001 | Human B Cell Culture and Expansion Kit | Used for activation and expansion of human B cells. |
BC1002 | 3T3-msCD40L Cell Lines | 3T3-CD40L cells can be co-cultured with B cells to aid B cell expansion. |
B8176 |
Z Cap™ Reagent AG, | Used for co-transcriptional capping of mRNA to form Cap 1 structure, m7G(5′)ppp(5′)(2’OMeA)pG. |