Elabscience specialise in immunodiagnostic technologies for the life science community.

Their product range includes proteins, antibodies, ELISA kits, CLIA kits and labelling kits.

Every single product undergoes in house QC ensuring consistent and precise experimental results.

Elabscience products are sold in more than 100 countries worldwide.


Product Literature

Technical Centre

Elabscience has collected a detailed list of over 8,000 alternative Santa Cruz antibodies to secure your supply of antibodies and continuation of research.

If you cannot find your antibody on the list below please contact our technical team technical@stratech.co.uk who can help!

SCBT Cat. No. Target Elabscience Cat. Reactivity Application
sc-7122 BCL-x E-AB-40057 Human,Mouse,Rat WB,IHC
sc-6461 Cadherin-8 E-AB-40086 Human WB
sc-21578 CAMP E-AB-40068 Human,Mouse,Rat WB,IHC
sc-22169 CASP2 E-AB-40077 Human WB
sc-7056 CD46 E-AB-40059 Mouse,Rat IHC
sc-31191 CD48 E-AB-40042 Human,Rat IHC
sc-954 CDK1 E-AB-40071 Human WB
sc-163 CDK2 E-AB-40095 Human,Mouse WB,IHC
sc-17113 CK-20 E-AB-40067 Human,Mouse,Rat WB,IHC
sc-79916 COX5B E-AB-40083 Human,Mouse,Rat WB,IHC
sc-18304 CRP E-AB-40005 Rat WB,IHC
sc-16989 CST3 E-AB-40010 Rat WB,IHC
sc-31857 ENO1 E-AB-40064 Human,Mouse,Rat WB,IHC
sc-1310 EPO E-AB-40008 Rat IHC
sc-7888 IL10 E-AB-40072 Mouse,Rat IHC
sc-48544 IL2 E-AB-40026 Mouse,Rat IHC
sc-323975 IL6 E-AB-40073 Mouse,Rat IHC
sc-48408 Lep E-AB-40003 Rat IHC
sc-8196 MYD88 E-AB-40054 Human WB
sc-471 P21 E-AB-40097 Human WB
sc-1985 PPARA E-AB-40069 Human WB
sc-65598 Proliferating Cell Nuclear Antigen E-AB-40056 Human,Mouse,Rat WB,IHC
sc-27992 SDHA E-AB-40089 Human,Rat WB,IHC
sc-8332 SMAD3 E-AB-40050 Human,Rat WB,IHC
sc-53607 STAT1 E-AB-40052 Human,Rat WB,IHC
sc-34543 TGM2 E-AB-40088 Human,Mouse WB
sc-7814 TSHB E-AB-40009 Mouse,Rat WB,IHC
sc-79036 UQCRFS1 E-AB-40082 Human,Mouse WB,IHC
sc-152 VEGFA E-AB-40004 Rat WB,IHC

The Elabscience® Protocols consist of collection of guidelines and frequently asked questions about the most commonly used immunology experiments. We built this forum to create a platform that can be used as reference for practical scientific experiments. We will continue to update and provide more technical information and practical resources.

Antibody WB Operation Guide Video

Sandwich ELISA Operation Guide Video

Antibody IHC Operation Guide Video

Elabscience Company Introduction Video

Are there any cautions for the label?

 The label should contain free primary amines or N-terminal, and the sample should not contain Tris, glycerin, and reagents with amino.

Could this biotin labeling kit be used for labeling other proteins in addition to antibodies?

 The principle of biotin labeling kit is the formation of stable amide bond between activated Biotin and amino of label. In addition, N-terminal of protein can be labeled. Protein contains primary amines, such as lysine, can be labeled.

When labeling protein with this kit, does the reaction ratio is the same proportion of 20:1 as labeling antibodies?

 The molecular weight and lysine amount of protein should be considered. In general, it is recommended to label according to the concentration of 2 mg/mL. If the amount of lysine is about 10~15, the reaction ration can be 1:20. The proportion will be increased if the amount of lysine is more.

Can this kit be used for small or trace labeling?

 The assay procedure in the manual is used for labeling 0.1~2 mg antibody. If you need to mark a smaller amount, we have a special trace labeling method used to label 20-100 μg antibody and the reaction system is 100 μL. (So the amount of Biotin should be increased if the labeling concentration is less than 2 mg/mL.)

How to detect the labeling efficiency?

 The labeling kits on sale have their optimized labeling method. The labeling efficiency of these kits are generally above 90% from our test. In addition, antibodies in our ELISA kits are also labeled with Biotin labeling kit. The biotin labeling efficiency can be determined by detecting the biotin amount with our Biotin Detection Kit.

Can the Filtration tube in this kit be repeatedly used?

 The Filtration tube in this kit can be repeatedly used no more than 3 times, but must be washed after the assay finished use the following method: Add purified water and gently pipetting for several times. Then add 0.2M NaOH, incubate for 10 minutes and centrifuge. Wash the tubes with purified water. The tube can put in the water for short term storage, while it should be preserved in 20% ethanol for long term storage. The used tubes should avoid of dry and keep moist.

How long is the shelf life of the activated biotin after dissolution?

 The unopened freeze-dried biotin powder can be stored stably for 1 year at 4 ℃, and it can be stored for 4 weeks in the dark if sealed properly after opening/dissolving..

How to judge whether some specific substance (e.g., folic acid) can be labeled with FITC or not?

The target substance can be labeled if it contains free amine, and the amount of primary amine (lysine) should be considered for proteins. Small molecules can be determined according to the amino number. The containing nitrogen heterocyclic ring of folic acid contains 1 amine, so it can be labeled with FITC theoretically. But the amine on this site has a weak nucleophilicity, the labeling effect may not good and it should be judged according to the purpose of their experiment by customers.

Is the protein with a molecular weight of 3.8 kD suitable to be operated with a 3 kD Filtration tube?

 No. If your target protein has a molecular weight of 3.8 kD, you have to choose the Filtration tube with a blocked diameter of less than 3.8/3, otherwise there is possibility of skip or plugging aperture.

If my target protein has 43 lysine, but I just want to label less than 5 biotin molecules. Is it necessary to take quality control labeling? Are there any methods to verify the labeling effect and amount?

 Considering the spatial configuration, the reaction ratio optimized by Elabscience is 1:20, so that 1 antibody will be labeled with 3~6 Biotin molecules. If you want to control the labeling amount to be less than 5, you should control the molar ration and take pre-experiment, and estimate the labeling amount with Our Biotin Detetcion Kit.

What concentration of primary antibody should I use?

For antibodies which the suggested working dilutions are listed as “Assay Dependent” this simply means that the optimal dilution should be obtained by the end user. However, we are always available to provide assistance.

Can I receive a free sample of a product?

 Generally, we do not offer free or trial sized samples for testing purposes. Our policy is that if an antibody does not work as specified on the datasheet, we will offer a replacement or refund (Except the promotion period. Detailed promotion information will be displayed on our website, or you can contact our sales). If the antibody is being used in an untested species or application, we cannot offer a replacement or refund.

How can I determine whether an antibody may detect in an untested species?

– Elabscience are unable to guarantee that an antibody will work in an untested species, even if the sequence alignment is high. There are many variables involved in the determining whether an antibody will bind in another species.

– If there are no alternatives available, and it is necessary for you to consider purchasing an antibody for use in a species that is not tested, we recommend checking the sequence alignment of the immunogen with the protein you are interested in.

Is the information on the datasheet up-to-date and correct?

 The datasheets contain the most up-to-date information we have available to us about this product. Where we find additional information, the datasheets are updated live on the website immediately,and you can contact our service team for any questions about Elabscience datasheet.

How should I choose a positive control?

 If you require a suitable positive control, please contact our technical support team and we would be happy to help. Additionally, make sure that the cell line or tissue being used is from one of the species listed on the data sheet.

What antigen retrieval method should I use with this antibody?

 We do not always have information available on the most suitable antigen retrieval method for an antibody in immunohistochemistry. This will often require a certain amount of optimization by the end user. Visit our IHC protocols page for more information on antigen retrieval methods.

Instrumentation sample preparation and storage for ELISA assays

How to deal with tissue or cells?

Normally, the amount and ratio of each reagent a fully automated microplate ELISA analyzer needs are not the same as in our kit, so it’s not applicable. Whereas if you can tell Cell lysates: For adherent cells, gently wash the cells with moderate amount of pre-cooled PBS and dissociate the cells by trypsin. Collect the cell suspension into the centrifugal tube and centrifuge for 5 minutes at 1000×g. Discard the medium and wash the cells for 3 times with pre-cooled PBS. For each 1×106 cells, add 150-250uL of pre-cooled PBS (0.01M, pH=7.4) to keep the cells resuspended. Repeat the freeze-thaw process for several times until the cells are lysed fully. Centrifuge for 10minutes at 1500×g at 2 – 8℃. Remove the cell fragments, collect the supernatant to carry out the assay. Avoid repeated freeze-thaw cycle.
Cell culture supernatant: Collect samples and centrifuge for 20 minutes at 1000×g at 2 – 8℃. Collect the supernatant to carry out the assay.
Tissue homogenates: Generally, mince the tissues to small pieces and rinse them in ice-cold PBS (0.01M, pH=7.4) to remove excess blood thoroughly. Tissue pieces should be weighed and then homogenized in PBS (tissue weight (g): PBS volume (mL) =1:9) with a glass homogenizer on ice. To further break the cells, you can sonicate the suspension with an ultrasonic cell disrupter or subject it to freeze-thaw cycles. The homogenates are then centrifuged for 5 minutes at 5000×g at 2 – 8℃, collect the supernatant to carry out the assay.Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g at 2 – 8℃ within 30 minutes of collection. Collect the supernatant to carry out the assay. Hemolysis samples are not suitable for ELISA assay.

How to collect serum and plasma?

Reading at dual wavelengths is to correct the optical density contributed by the plate wells and other nonspecific interference. Our plates are chosen for their optical quality, Serum: Allow samples to clot for 2 hours at room temperature or overnight at 4℃ before centrifugation for 15 minutes at 1000×g at 2 – 8℃. Collect the supernatant to carry out the assay. Blood collection tubes should be disposable, non-endotoxin.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g at 2 – 8℃ within 30 minutes of collection. Collect the supernatant to carry out the assay. Hemolysis samples are not suitable for ELISA assay.

Saliva, Urine, milk, tear and other types of biological fluids

In general, use a sterile container to collect fluids, then centrifuge it at 1000-5000×g at 2 – 8℃ for 5-20min to remove particulates, collect the supernatant to carry out the assay.

Storage

Samples should be assayed within 7 days when stored at 2-8℃, otherwise samples must be divided up and stored at -20℃ (≤1 month) or -80℃ (≤3 months). Avoid repeated freeze-thaw cycles. Centrifuge again before assaying to remove any additional precipitates that may appear after storage.

How to perform an ELISA assay as perfectly as possible?

How to calculate/ deal with the raw datas?

After correct the average OD values, plot a four parameter logistic curve on log-log graph paper, with standard concentration on the x-axis and OD values on the y-axis, many softwares could also help to deal with it, such as Origin( recommended), CurveExpert.

Target analyte in tested samples

 It’s recommended to test fresh samples by ELISA, which could avoid false positive or negative results caused by the degradation or decomposition of target analyte during long time storage.

Dilution factors and diluent

 Dilution ratio: Refer to the specific protocol or published papers, or consult to our technical support.

Recommended diluent: Standard & Sample Diluent provided in the ELISA kit, we could provide more vials of it for free for specific application, just tell us when you place the order.

Assay procedure

Following the protocol strictly, here are some hints
a. Establish the complete Standard Curve for each assay.
b. Ensure the precise volume for each reagent addition
c. Try to finish the liquid addition for each reagent as soon as possible, better not over 10 min.
d. Keep constant speed for liquid addition to each well
e. To get more accurate experiment result, it’s recommended to conduct the ELISA assay as soon as possible once you receive the kit.

FAQs for ELISA

PolyPeptide dissolution

Dissolving a polypeptide is a very complex process, and it is generally difficult to determine a suitable solvent quickly. Usually take a little for a preliminary test, and do not dissolve completely before determining the proper solvent.

The following methods can help you choose the right solvent:

 Determine the charge specificity of the polypeptide, set the acidic amino acid Asp (D), Glu (E) and C-terminal COOH to -1; basic amino acid Lys (K), Arg (R), His (H) and N-terminus NH2 is +1 and the charge of the other amino acids is zero. Calculate the net charge number.

 If the net charge number is >0, the polypeptide is alkaline and dissolved in water; if the peptide does not dissolve, add 10% acetic acid dropwise with vortexing. The peptide solution can also be warmed slightly. Longer peptides (over 20 amino acids) with a small overall net charge might require the addition of a stronger acid. Trifluoroacetic acid (TFA 10-50 µL) is often used to solubilize peptides but it is not cell-friendly and thus is used only when acetic acid fails to help solubilize the peptide. After the addition of TFA, the peptide should be diluted to approximately 1 mL with deionized water.

 If the net charge number is <0, the peptide is acidic and dissolved in water; If the peptide does not dissolve, add ammonium hydroxide (NH4OH 10-50 µL), and dilute the peptide to approximately 1 mL with deionized water.

Note: Caution must be used, however, with peptides that contain cysteine (C), as the used of alkaline pH can cause disulfide bond formation.

 If the net charge number = 0, the polypeptide is neutral and generally needs to be dissolved with an organic solvent such as acetonitrile, methanol or isopropanol, DMSO and the like. It has also been suggested that urea is required to dissolve highly hydrophobic polypeptides.

PolyPeptide preservation

Generally long-term preservation of polypeptides need to avoid light, and should be stored below -20 ℃, short-term can be stored at 4℃. It can be transported at room temperature for short periods of time. Polypeptides are very stable at -20℃, especially when they are lyophilized and stored in a desiccator. Before they are exposed to air, lyophilized polypeptides can be naturally heated up at room temperature, which reduces the effect of humidity. When lyophilizing is not possible, the best way is to store it in small amount of working samples.

For polypeptides containing Cys, Met or Trp, deoxidation buffers are essential for their dissolution, as the polypeptides are prone to air oxidation, and nitrogen or argon protection can reduce oxidation before sealing. Polypeptides containing Gln or Asn are more susceptible to degradation and have a shorter shelf life than normal peptides that do not contain these problematic amino acid.

Elabscience always adheres to the concept of “Focus on your research, Service for life science”. After years of rapid development, it has become a rising star in the field of global bio-reagent customization services. The business scope covers life science research such as gene synthesis, peptide synthesis, protein expression and antibody development, and is widely recognized by customers and the market.

Advantages

Custom Gene Synthesis Service

  •  Free codon optimization
  •  High-throughput
  •  Short turn-around time
  •  100% sequence accuracy guaranteed

Custom Peptide Synthesis Service

  • MS and HPLC test report
  •  Variety of peptide modification
  •  Over 95% success rate
  •  Rush peptide in 1 week

Custom Protein Service

  •  Comprehensive expression systems
  •  Fast turn-around
  •  One-stop service platform
  •  Competitive price

Custom Polyclonal Antibody Service

  •  Large-scale antibody production capability
  •  Various host species available
  •  ELISA titer guarantees
  •  Western-guaranteed antibodies

Gene synthesis, sometimes known as DNA printing, is a method in synthetic biology that is used to create artificial genes in the laboratory. Based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that it does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size.

Advantages

  •  Short turn-around time.
  •  100% sequence accuracy guarantee.
  •  Site directed mutagenesis service.
  •  Gene Synthesis Service covering any Length or Complexity
  •  Free codon optimization and free cloning into many standard vectors.
  •  Over 95% success rate with “No Gene, No Charge” policy. No hidden costs and no setup charges.

Price and delivery time

Length Size Price Typically Shipped
Minigene 25-500bp 4 µg Please Inquire 5 business days
Gene 501-8000bp 4 µg 501-1000bp   7 business days
4 µg 1001-2000bp  7-10 business days
4 µg 2001-5000bp  10-20 business days
4 µg 5001-8000bp  20-25 business days
Gene >8000bp 4 µg Please Inquire

Note: Genes ≤8000 bp will be delivered dry in a circularized plasmid and normalized to 4 µg final yield.* For genes ˃8000 bp, please contact us.

Elabscience® provides peptide synthesis service of different quality and purity range. The quality can be from mg, g to kg. The purity covers crude, desalted, 70%, 75%,80%,85%,90%,95% and 98%.

Advantages

  • Up to 100aa peptide sequences can be synthesized
  •  Variety of peptide modification services
  •  Over 95% of synthesis success rate
  •  Fastest peptide synthesis speed in the industry
  •  All peptides are analyzed by MS and HPLC

Modifications

Elabscience offer a wide range of modifications to meet any research need.

  •  N-Terminal Modifications
  •  C-Terminal Modifications
  •  D-Amino Acids
  •  Unnatural/Unusual amino acids
  •  Fluorescence/Dye Labeling
  •  Stable isotope labeled peptides
  •  Pegylation
  •  Cyclic peptide
  •  Peptide conjugates

*Please contact us for the prices of different modifications.

Applications

  •  Antibody epitope mapping
  •  Monoclonal and polyclonal antibody production
  •  Non-quantitative enzyme-substrate studies
  •  Biological effects of defined peptides
  •  Blocking and competition assays of proteases
  •  New techniques for separation by chromatography/electrophoresis
  •  Immunization and quantitative receptor-ligand interactions
  •  Antibody-antigen interactions
  •  ELISA standards for measuring titers of antibodies
  •  Epitope mapping Structure, dynamics, and folding of peptides and proteins via NMR

protein service

Elabscience’s custom recombinant protein services provide a comprehensive portfolio of protein expression systems, including bacteria, yeast, baculovirus infected insect cells, and mammalian cells. We offer the ideal level of protein purity for your downstream applications. With customized solutions, flexible production scale, and proprietary technologies, we work in partnership with our clients to ensure efficient and high quality reagent protein production, process development and scale-up production of biologics for research purposes.

Elabscience® has delivered over 2,000 batches of recombinant proteins used for functional assays, high throughput screens, antigen generation, structural analysis, and many other drug discovery applications. When our extensive expertise in recombinant proteins is integrated with our in-house gene synthesis and assay development capabilities, we can quickly deliver your projects in a flexible and cost-effective manner. Moreover, our dedicated technical account managers will guide your project through every step of the process, and constantly keep you informed of the project progress.

Advantages

  •  Comprehensive expression systems: Bacteria, yeast, baculovirus/insect cell, and mammalian cell with various vectors for each system.
  •  Fast turn-around: 10 mg of purified protein within 5 weeks merely from you submitted your target gene sequence.
  •  One-stop service platform: Elabscience® can take your project directly from gene synthesis, to any of those further stages including recombinant protein expression, antibody production, and assay development.

Available Protein Services

  •  Yeast Expression System
    Proprietary Technologies / Humanized antibody production Streamlined Services
  •  Bacterial Expression System
    Competitive price / Fast turn-around / Flexible scale-up protein production / Guaranteed Package

Unlike monoclonal antibodies, polyclonal antibodies correspond to multiple epitopes and tend to contain several immunoglobulin subtypes. They can be used as both primary and secondary antibodies in Westerns, ELISAs, and IHCs.

Elabscience® provides polyclonal antibody services in mouse, rat, rabbit, and other species. Our polyclonal antibody packages include antigen design, peptide synthesis, immunization, purification, ELISA test, and lyophilization. You can simply select your desired packages and send us the specific requirements, and then Elabscience® will offer antibodies with guaranteed quality.

Advantages

  •  Multiple antibody labeling for alternatives: Elabscience® offers multiple antibody labeling for you to choose, including Biotin, HRP, FITC, Cy3, Cy5, APC, etc.
  •  Flexible service portfolio: Different host species (mouse, rat, and rabbit) and purification method (Protein A/G purification/ Antigen Affinity purification) are available for your research demands.
  •  Personalized experiment scheme: Elabscience’s experienced antibody experts will design the personalized experiment scheme according to your specific requirements and applications.
Cat.No.Service NameTimeline
S-CA-P001Peptide synthesis and conjugation3 weeks
S-CA-P002Protein expression6-7weeks
S-CA-P003Polyclonal antisera from rabbit12 weeks
S-CA-P004Polyclonal antisera from mouse12 weeks
S-CA-P005Polyclonal antisera from rat12 weeks
S-CA-P010Protein A/G purification1 week
S-CA-P011Antigen Affinity purification1 week
S-CA-P012Antibody labeling (Biotin)1 day
S-CA-P013Antibody labeling (HRP)2 days
S-CA-P014Antibody labeling (FITC)1 day
S-CA-P015Antibody labeling (Cy3)1 day
S-CA-P016Antibody labeling (Cy5)1 day
S-CA-P017Antibody labeling (APC)2 days
Quotations and Ordering

Polyclonal Antibody Service Quotations and Ordering

Products

Featured Products

Super Plus High Sensitive and Rapid Immunohistochemical Kit(pH9.0) (E-IR-R220)

Elabscience® Super Plus High Sensitive and Rapid Immunohistochemical Kit is a high sensitive and rapid immunohistochemical broad spectrum detection reagent. There is no need to do the traditional three times of xylene dewaxing, three to five times of gradient ethanol hydration or antigen repair but only one Dewaxing/Antigen Retrieval Buffer heating without fume hood or traditional dewaxing/antigen repair tanks. The operation time is effectively shortened, the cumbersome operation steps are simplified so the variables in operation are reduced, and the stability is improved. The Dewaxing/Antigen Retrieval Buffer applies new environmental protection technology is not only reduces the harm to the body, but also cause no pollution to the environment.

This kit is easy to operate and fast because of the one-stop reagents used in the immunohistochemical experiment, and the high sensitive secondary antibody/ DAB detection reagents make this kit to be more sensitive than other produce.

This kit can be used to detect monoclonal/polyclonal antibody derived from Rabbit and Mouse.

Components:

Catproduct3mL10mLStorage
SP Reagent 9ADewaxing/Antigen Retrieval Buffer(pH9.0) (20×)60 mL120 mL2~8°C
SP Reagent BPeroxidase Blocking Buffer3 mL10 mL2~8°C
SP Reagent CPolyperoxidase-anti-Rabbit/Mouse IgG3 mL10 mL2~8°C
SP Reagent DHigh Sensitive DAB Concentrate (20×)150 μL500 μL2~8°C
SP Reagent EHigh Sensitive DAB Substrate3 mL10 mL2~8°C
SP Reagent FHematoxylin Staining Buffer3 mL10 mL2~8°C
SP Reagent GAntibody Dilution Buffer3 mL10 mL2~8°C

Storage:

Store at 2~8°C, away from light. Avoid of freezing. Valid for 12 months. The reagents are valid within 6 months after opening.

QuicKey ELISA® Kit , the newly developed technology, saves at least 1h for the ELISA assays, and only requires 50μL for sample addition. It shortens the experiment time and saves your precious samples without compromising high sensitivity, specificity, accuracy and stability. The new kits cover the most popular research targets of renal function check and inflammation detection, guarantee the experiment performance and greatly improve the detection efficiency!

Why Choose QuicKey ELISA® Kits?

  • Save 1h at least
  • More simplified assay procedures
  • Higher stability, easier storage
  • More cost-effective, lower price

Targets for research

Renal Function Biomarkers
TF, NGAL, β2-MG, MAU, RBP4, Cys-C, CLU

Inflammation Biomarkers
IgG, CRP, TGF-α, PCT, ALB, Acrp30, IgA, IgE, IgM, TG, IgG (Mouse)

Signal Transduction
VEGF-A, TGF-α, Acrp30

Test principle of QuicKey ELISA® kits

Conventional ELISAQuicKey ELISA®What it means for you?
Assay time3.5h2.5hSave 1h
Sample volume100μL50μLSave samples
Assay proceduresIncubate sample and detection Ab separatelyIncubate sample and detection Ab simultaneouslySimpler operations
StorageComponents need to be separately stored at -20℃ or 4℃Whole kit can be kept at 4℃More convenient storage
HRP conjugateThe working solution is colorlessThe working solution is redLess risks of mistakes

Elabscience® has developed a series of metabolism assay kits with simple operation, high sensitivity and excellent quality through the method of enzyme reaction with fluorescent probe, allowing you to obtain accurate results in less time and samples. Elabscience® fluorometric assay kits have applications in Oxidative stress, Liver biomarkers and Kidney biomarkers, Glycolysis, Lipids metabolism, etc. Fluorometric assay kits are suitable for a variety of sample types, involving a variety of body fluids, tissue and cells. The main fluorometric detection instrument is the fluorescence microplate reader. Fluorometric assay kits can help you to upgrade your metabolism assay experiments!

Why Do You Want to Choose Fluorometric Assay Kit?

Fluorometric Assay Kit Advantages

Cost-effective

Provide dilution factor of the sample

Improve experimental efficiency, Fewer experiments )

 

Sample type Dilution factor Sample type Dilution factor
Human serum 10-20 10% Rat liver tissue 200-400
Rat serum 200-400 10% Mouse kidney tissue 200-400
Mouse plasma 100-300 10% Rat brain tissue 200-400
Rabbit serum 100-300 10% Rat lung tissue 200-400

Fluorometric Assay Kit Targets

Cat. No. Product Name Size
E-BC-F001 Hydrogen Peroxide (H2O2) Fluorometric Assay Kit 48T/96T
E-BC-F002 Adenosine Triphosphate(ATP) Chemiluminescence Assay Kit 48T/96T
E-BC-F006 Catalase (CAT) Fluorometric Assay Kit 96T
E-BC-F013 Myeloperoxidase (MPO) peroxide activity Fluorometric Assay Kit 96T
E-BC-F018 Uric Acid (UA) Fluorometric Assay Kit 48T/96T
E-BC-F019 Xanthine Oxidase (XOD) Activity Fluorometric Assay Kit 96T
E-BC-F032 Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit 48T/96T
E-BC-F037 Glucose (GLU) Fluorometric Assay Kit 48T/96T
E-BC-F038 Alanine Aminotraasferase (ALT) Fluorometric Assay Kit 48T/96T
E-BC-F039 Non-esterified Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit 48T/96T
E-BC-K138-F Reactive Oxygen Species (ROS) Fluorometric Assay Kit 96T
E-BC-K298-F Thiobarbituric Acid Reactants (TBARS) Fluorometric Assay Kit 48T/96T

FAQs

Lower readings in samples and standards

Please be sure to verify the expiration date and store the kits properly, at the meantime you can check whether the parameter page is the top reading or not.

 

How to dilute the sample?

Sample dilution factor provided in the manual can be referred. If not, you can select three dilution multiples of high, medium and low for pre-experimental determination according to the results in the literature.

Why is the fluorescence value of standard significantly different from those provided in the manual?

Different instruments use different methods, so there is no comparability between the fluorescence value measured by different instruments, but this won’t affect the measurement results.

The importance of antibody reproducibility

In recent years, researchers have found that there has been a serious “repeated crisis” in antibody related research.

According to the statistics of Nature, 80% of researchers can’t repeat other researcher’s experimental results, and 60% can’t repeat their own experimental results.

The quality problem of antibody is one of the main culprits leading to the unrepeatable experiment. The human protein atlas project has tested 9,000 antibody products from 29 suppliers, and more than 50% of them fail to work effectively.

It is estimated that US $350 million and US $800 million are wasted annually on the use of unidentified antibodies in the United States and around the world, such antibodies can lead to experimental failure or unreliable results.

The quality of antibody is one of the main reasons leading to unrepeatable experiments.

At present, the American Society of Pathologists and Laboratory Quality Center released a guideline on the analysis and verification principles of IHC testing (Principles of Analytic Validation of Immunohistochemical Assays), at the CAP2014 annual meeting in the United States. It was recently published in the Arch Pathol Lab Med., the article clearly pointed out the necessity of routine IHC testing for cytology samples.

In scientific research, different scientific researchers have slight differences in operating methods, reagent configuration, and experimental details. In addition, the poor repeatability of antibodies can easily cause different degrees of incompatibility and cause uncontrollable experimental results. In order to minimize this uncontrollability, Elabscience® combined with Lifespan’s immunohistochemical quality inspection platform to perform a secondary immunohistochemical quality inspection on the antibodies produced by Elabscience®. To screen high-quality immunohistochemistry antibodies according to Lifespan’s quality inspection standards, and provide more professional inspections for your immunohistochemistry experiments. In addition, Elabscience® provides you with a complete set of immunohistochemistry solutions, one-stop related reagents to solve your problems.

Antibody validated by IHC

Immunohistochemistry (IHC) is a highly useful technique for detection and quantification of target epitopes (e.g. proteins, structures, cellular components) in a wide variety tissue types. Applicable to fresh, frozen and FFPE samples, IHC staining utilizes high specificity antibodies for visualization of target expression patterns, providing quantitative, qualitative and spatiotemporal information on processes taking place in tissues. Antibody-epitope interactions are visualized through secondary staining of samples with an additional antibody that has been conjugated to either a color-producing enzyme (chromogenic staining) or a fluorophore (immunofluorescent staining, or IF).

In the future, we will display more validated antibodies by IHC, and plan to use different validation methods to improve antibody standards.

If you are interested in our process of verifying antibodies or our standards for antibody development, please find more information on our website.

Elabscience® IHC validated antibody

Cat.No. Product Reactivity Applications Validated Tissues
E-AB-40032 AKT1 Polyclonal Antibody M,R IHC Human prostate
E-AB-40114 ANXA5 Polyclonal Antibody H,M,R WB,IHC Human prostate
E-AB-12728 APOL1 Polyclonal Antibody H WB,IHC,ELISA Human liver and Human thymus
E-AB-12977 APOL2 Polyclonal Antibody H WB,IHC,ELISA Human placenta
E-AB-13047 ASPN Polyclonal Antibody H,M WB,IHC,ELISA Human testis
E-AB-10995 BCAT1 Polyclonal Antibody H,M IHC,ELISA Human liver
E-AB-40057 BCL-x Polyclonal Antibody H,M,R WB,IHC Human spleen
E-AB-10185 BMP4 Polyclonal Antibody H,M,R WB,IHC,ELISA Human smooth muscle
E-AB-13098 BST1 Polyclonal Antibody H,M,R IHC,ELISA Human heart
E-AB-70027 c-Fos Polyclonal Antibody H,M,R IHC Human liver
E-AB-70005 Catenin beta Polyclonal Antibody H,M,R WB,IHC,IF Human colon
E-AB-10208 Cathepsin B Polyclonal Antibody H,M,R IHC,ELISA Human liver
E-AB-13973 Cathepsin B Polyclonal Antibody H,M,R IHC,ELISA Human liver
E-AB-40261 CBX5 Polyclonal Antibody H,R IHC Human tonsil
E-AB-12309 CCL17 Polyclonal Antibody H,M WB,IHC,ELISA Human spleen
E-AB-40247 CCNL2 Polyclonal Antibody H IHC Human testis
E-AB-15992 CD10 Polyclonal Antibody H,M,R IHC,ELISA Human kidney
E-AB-10262 CD151 Polyclonal Antibody H,M,R IHC,ELISA Human kidney and Human placenta
E-AB-70018 CD19 Polyclonal Antibody H IHC,IF Human spleen
E-AB-40044 CD207 Polyclonal Antibody H,M IHC Human skinLangerhans

Cancer Research Antibodies

At present, more than 30 antibodies or antibody derivatives have been approved for cancer treatment by FDA, and hundreds of antibodies are in the process of clinical trials. Antibodies have become a new choice for cancer treatment. Anti-cancer antibodies can target a wide range of antigens, including soluble proteins, cancer cell surface antigens and effector cell receptors.

The versatility of antibodies enables antibodies to take a variety of mechanisms, including direct blocking of growth factors, regulation of the immune system, and interruption or induction of signal transduction pathways. In addition, antibodies are increasingly used as carriers to improve the specificity of specific chemotherapeutic drugs.

Advantages of Elabscience ® Cancer Antibodies

  • High antigen purity:The purity of each recombinant protein used as an immunogen is ≥90%, part of which are ≥ 95%.
  • High antibody specificity:Antibodies with high affinity antigen specificity were obtained by antigen affinity purification.
  • Full and accurate data of cancer antibodies:WB experimental operation guidelines include all the experimental conditions in the WB experimental process. The products have been strictly verified and the datas are accurate and reliable.
  • Comprehensive and accurate experimental data:Multi species and applications quality detection to ensure that each antibody is a high-quality product that has been strictly screened.

Cancer Research Antibodies

  • Lung Cancer
  • Colorectal Cancer
  • Gastric Cancer
  • Reproductive System Cancer
  • Breast Cancer
  • Liver Cancer
  • Esophageal Cancer
  • Pancreatic Cancer
  • Thyroid Cancer
  • Lymph Cancer
  • Leukemia
  • Cardio-cerebrovascular Disease

Popular Antibodies in cancer research

PTEN– Tumor suppressor gene

PTEN-- tumor suppressor gene

PTEN is a widespread and closely related tumor suppressor gene, which has abnormal expression in glioblastoma, breast cancer, lung cancer, bladder cancer, thyroid cancer, head and neck squamous cell carcinoma, melanoma, lymphoma and other tumors.

POSTN—Periostin
POSTN—Periostin

POSTN is an extracellular matrix periosteal protein, which can support the growth of transferase in the formed microenvironment by enhancing Wnt signaling in tumor cells. In adults, it is expressed in specific organs such as breast, bone, skin and intestine.

AFP– Alpha fetoprotein

AFP-- Alpha fetoprotein

Alpha fetoprotein is closely related to the occurrence and development of liver cancer and many kinds of tumors. It shows high concentration in many kinds of tumors and can be used as a positive detection index for many kinds of tumors.

EGFR– Epidermal growth factor receptor

EGFR-- Epidermal growth factor receptor

There are high or abnormal expression of EGFR in many solid tumors. EGFR is related to tumor cell proliferation, angiogenesis, tumor invasion, metastasis and inhibition of apoptosis.

CK-18– Cytokeratin 18
CK-18-- Cytokeratin 18

CK18 is a normal cytoskeleton component of hepatocytes and a standard of tumor markers. CK18 levels were increased in patients with benign liver disease, especially those with significant cytolysis.

IL-6R– Interleukin-6 receptor
IL-6R-- Interleukin-6 receptor

IL-6R can specifically combine with IL-6 to block the related diseases caused by IL-6 and reduce the severity of asthma, multiple sclerosis, chronic rheumatoid arthritis and other diseases.

One-step TUNEL Assay Kit

Elabscience® improves the process of the entire line of products, and offers you a highly sensitive, fast and simple apoptosis detection kit (TUNEL) at a lower price and a better product experience, which can better assist in apoptosis research on diseases related to cell function and research and development of related drugs.

This kit is suitable for in situ apoptosis detection of tissue samples (Paraffin embedding, frozen section) and cells (Cell smears, cell climbing smears) in situ apoptotic detection. The test results can be directly observed through a fluorescence microscope.

Detection Principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. After extraction and loading for electrophoresis, a DNA ladder of 180-200 bp can be found in such DNA samples, which is a specific event during cell apoptosis. The exposed 3′-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with labeled dUTP, which can be detected with fluorescence microscopy or flow cytometry.。

Applications

Functional study of cell apoptosis

  • The effect of gene up-regulation or down-regulation on cell function
  • The effect of drugs on the function of cancer cells
  • The effect of viruses on cell function

Research on apoptosis-related diseases

  • Insufficient apoptosis: tumors, autoimmune diseases, viral infections
  • Excessive apoptosis: cardiovascular disease, blood system disease, severe hepatitis, degenerative neurological disease

Why Choose Elabscience® One-step TUNEL Assay Kits?

  • Safety: The new buffer does not contain cacodylate, which is safe and non-toxic.
  • Multi-color:There are red, green, blue and other colors to choose from, and can be customized for more needs
  • Efficiency:Only one step of dyeing reaction, fast and simple
  • Accuracy: High sensitivity, low background, good specificity, strong signal
  • Stability: Strict quality control to ensure repeatability between batches
  • High quality: The quality is equivalent to TUNEL products of other commercial available brands

Example Data Analysis

Formalin-fixed, paraffin embedded (FFPE) mouse enteritis tissue were stained with Elabscience® One-step TUNEL Assay Kit (code – E-CK-A321)  (Green, AF488) [left] or other commercial TUNEL Assay Kit (Green) [right]

Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained with Elabscience® One-step TUNEL Assay Kit (cat no. E-CK-A321) (Green, AF488) [empty black histogram] or other commercial TUNEL Assay Kit (Green) [filled gray histogram]

Elabscience® One-step TUNEL Assay Products

Product Name Cat. No. Size
One-step TUNEL Assay Kit (Green, FITC) E-CK-A320 20 Assays/ 50 Assays/ 100 Assays
One-step TUNEL Assay Kit (Green, AF488) E-CK-A321 20 Assays/ 50 Assays/ 100 Assays
One-step TUNEL Assay Kit (Red, AF594) E-CK-A322 20 Assays/ 50 Assays/ 100 Assays
One-step TUNEL Assay Kit (Blue, EV450) E-CK-A323 20 Assays/ 50 Assays/ 100 Assays
One-step TUNEL Assay Kit (Red, AF647) E-CK-A324 20 Assays/ 50 Assays/ 100 Assays

After more than ten years of dedicated research and development, Elabscience® can provide you with Western Blot, IHC, IF, ELISA and other immunological reagents, to provide you with a complete set of solutions, one-stop related reagents, to meet your various laboratory needs.

FCM Related Reagents

FCM Related Reagents

ELabscience® provides auxiliary reagents needed in FCM experiments,Help you complete the experiment more efficiently!

ELISA related reagents

ELISA related reagents

Elabscience®provides ancillary reagents required for ELISA kit developments.

11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.

Labeling Kits

Labeling Kits

Elabscience®developed perfect protein and antibody labeling technology,

We provide high efficiency fluorescent labeling kits and biotin labeling kits!

WB Related Reagents

WB Related Reagents

From sample preparation to blocking, from antibody incubation to result detection, all the reagents you need are here!

And there are kits to meet your different needs!

IHC Related Reagents

IHC Related Reagents

Relevant reagents required for a complete IHC experiment!

One stop to meet the needs of all reagents in your immunohistochemistry experiment!

IF Related Reagents

IF Related Reagents

Elabscience®perfect immunofluorescence kit and reagents!

Fully meet your experimental needs!

Antibodies

Experiment Process

Cell Surface Targets Staining

Cell Intracellular Targets Staining

Elabscience® Hot Flow Cytometry Antibodies Products

Cell Subpopulation Species Target Cat. No. Product Name
T Cells Human CD3-PE E-AB-F1001D PE Anti-Human CD3 Antibody[OKT3]
CD4-FITC E-AB-F1109C FITC Anti-Human CD4 Antibody[RPA-T4]
CD8a-APC E-AB-F1110E APC Anti-Human CD8a Antibody[OKT-8]
Mouse CD3-PE E-AB-F1013D PE Anti-Mouse CD3 Antibody[17A2]
CD4-FITC E-AB-F1097C FITC Anti-Mouse CD4 Antibody[GK1.5]
CD8a-APC E-AB-F1104E APC Anti-Mouse CD8a Antibody[53-6.7]
B Cells Human CD3-FITC E-AB-F1001C APC Anti-Mouse CD8a Antibody[53-6.7]
CD19-APC E-AB-F1004E APC Anti-Human CD19 Antibody[CB19]
Mouse CD3-FITC E-AB-F1013C FITC Anti-Mouse CD3 Antibody[17A2]
CD19-APC E-AB-F0986E APC Anti-Mouse CD19 Antibody[1D3]
NK Cells Human CD3-FITC E-AB-F1001C FITC Anti-Human CD3 Antibody[OKT3]
CD16-PE E-AB-F1236D PE Anti-Human CD16 Antibody[3G8]
CD56-PE E-AB-F1239D PE Anti-Human CD56 Antibody[5.1H11]
BALB/c Mouse CD3-FITC E-AB-F1013C FITC Anti-Mouse CD3 Antibody[17A2]
CD49b-APC E-AB-F1116E APC Anti-Mouse CD49b Antibody[DX5]
C57BL/6 Mouse CD3-FITC E-AB-F1013C FITC Anti-Mouse CD3 Antibody[17A2]
NK1.1-APC E-AB-F0987E APC Anti-Mouse CD161/NK1.1 Antibody[PK136]
Th1/Th2/Th17 Cells Human CD4-ER780 E-AB-F1109S ER780 Anti-Human CD4 Antibody[RPA-T4]
IFN-γ-FITC E-AB-F1196C FITC Anti-Human IFN-y Antibody[B27]
IL-4-PE E-AB-F1203D PE Anti-Human IL-4 Antibody[MP4-25D2]
IL-17A-APC E-AB-F1173E APC Anti-Human IL-17A Antibody[BL168]
Mouse CD4-ER780 E-AB-F1097S ER780 Anti-Mouse CD4 Antibody[GK1.5]
IFN-γ-FITC E-AB-F1101C FITC Anti-Mouse IFN-γ Antibody[XMG1.2]
IL-4-PE E-AB-F1204D PE Anti-Mouse IL-4 Antibody[11B11]
IL-17A-APC E-AB-F1199E APC Anti-Mouse IL-17A Antibody[TC11-18H10.1]
Treg Cells Human CD4-ER780 E-AB-F1109S ER780 Anti-Human CD4 Antibody[RPA-T4]
CD25-PE E-AB-F1194D PE Anti-Human CD25 Antibody[BC96]
CD127-APC E-AB-F1152E APC Anti-Human CD127/1L-7RA Antibody[A019D5]
Mouse CD4-ER780 E-AB-F1097S ER780 Anti-Mouse CD4 Antibody[GK1.5]
CD25-PE E-AB-F1102D PE Anti-Mouse CD25 Antibody[PC-61.5.3]
FOXP3-APC E-AB-F1238E APC Anti-Mouse FOXP3 Antibody[3G3]

Polyclonal antibody is a group of immunoglobulin secreted by plasma cells in the organism, which occurred during the immune reaction when the body is stimulated by heterologous antigens (macromolecular antigen, hapten conjugates). Polyclonal antibodies have been widely applied in science research and diagnosis due to the various advantages, such as recognizing multiple antigen epitopes, causing precipitation reaction, short preparation time and low cost.

The most important quality for polyclonal antibodies is high titer. Elabscience’s polyclonal antibodies are prepared from the antiserum of immunized animal by affinity purification, and this preparation method leads to the production of interested antibodies with high-titer and high-affinity which against the target antigen. We have collected a wide selection of high-quality polyclonal antibodies specific for various species and applications, and all of them are guaranteed to do great favor for your research.

A monoclonal antibody specifically recognizes a single epitope within an antigen, thus having following advantages compared with polyclonal antibodies, low cross-reactivity, high specificity, excellent lot-to-lot stability.

Elabscience® offers Mouse Monoclonal antibody secreted by hybridoma cell which is fused with unique cell clones and malignant myeloma cells. They have the characteristics of high potency and stable quality. The Mouse Monoclonal antibody are with high potency and stable quality.

Elabscience® also provides custom monoclonal antibody service, designing and producing high quality antibodies according to your project requirements, to benefit your research at the greatest extent.

Malaria, Black Death, Spanish flu, Ebola virus, SARS and the 2019-nCoV have taken many people’s lives. In particular, 2019-nCoV is still spreading across the world. Every time when a new virus appears, scientists need to spend a lot of time looking for ways to deal with the virus.

As a global life science supplier, Elabscience® has been committed to providing scientific researchers with effective solutions and high-quality services to support their research on viruses, including 2019-nCoV, SRAS, MERS, HIV and HPV virus. Elabscience® virus-related antibodies and proteins are strictly tested to ensure their purity and quality to speed up your research process.

Human Coronavirus Antigens and Antibodies

Coronaviruses are a group of related RNA viruses that cause diseases in mammals and birds. In humans and birds, they cause respiratory tract infections that can range from mild to lethal. Mild illnesses in humans include some cases of the common cold (which is also caused by other viruses, predominantly rhinoviruses), while more lethal varieties can cause SARS, MERS, and COVID-19. In cows and pigs they cause diarrhea, while in mice they cause hepatitis and encephalomyelitis.

SARS-CoV-2(COVID-2019), which is a new coronavirus pneumonia, is the seventh known coronavirus that can infect humans. The remaining six human coronaviruses are HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, SARS-CoV and MERS-CoV.

Please click below to learn more product details.

Secondary antibodies can bind to primary antibody namely ‘anti-antibody’. Secondary antibody combines enzyme or fluorescent, and indirectly detects the target protein by binding with the primary antibody, thus amplifying the signal and improving the sensitivity of detection.

Elabscience® offers various high-quality conjugated/unconjugated secondary antibodies which can be applied in fluorescence detection of the primary antibody, and they are suitable for conventional applications such as IF, IHC, WB and Flow cytometry, etc.

The history of human society is a history that human beings constantly fight against viruses and overcome various viruses through knowledge innovation and technological progress.

Malaria, black death, Spanish flu, Ebola virus, SARS in 2003 and theCOV-2019 have taken many people’s live. In particular, COV-2019 is stilloutbreak in the global world and many families lost their love.

In the process of human civilization development, we are suffering from infectious diseases caused by viruses. Every time when a new virus appears, scientists need to spend a lot of time looking for ways to deal with the virus.

ELabscience® has been committed to providing effective solutions for researchers to supporting the study of infectious diseases. Which includes Cov-2019,SRAS-Cov,MERS-Cov and other virus like HIP virus,HPV virus,and so on.

These antigens and antibodies are specifically developed to help science researcher to learn more about virus and its infection mechanism.

Advantages of Elabscience® KO validated antibodies

Strict quality control
KO correlation verification was carried out to ensure the success of target knockout at the gene level and protein level.

Present stock
A manufacturer that launch KO validated antibodies.

Reliable product
The KO products can be directly tested to avoid cumbersome antibody data judgment and multiple validation of quality again.

Advantages of Elabscience® KO validated antibodies

HMGB1 Polyclonal Antibody
Cat.No.:E-AB-60726
Reactivity:Human,Mouse,Rat
Application:WB,IHC,IF

LC3B Polyclonal Antibody
Cat.No.:E-AB-61580
Reactivity:Human,Mouse,Rat
Application:WB,IHC,IF

DNMT1 Polyclonal Antibody
Cat.No.:E-AB-60994
Reactivity:Human
Application:WB,IF

FANCD2 Polyclonal Antibody
Cat.No.:E-AB-67288
Reactivity:Human
Application:WB

 

Cat Product Name Reactivity Application
E-AB-63251 KO Validated Fatty Acid Synthase Polyclonal Antibody Human WB,IHC,IF
E-AB-63262 KO Validated CCND3 Polyclonal Antibody Human,Mouse WB
E-AB-60129 KO Validated PARK7 Polyclonal Antibody Human,Mouse WB,IF
E-AB-62345 KO Validated YAP1 Polyclonal Antibody Human,Mouse,Rat WB,IHC,IF
E-AB-62198 KO Validated ROCK1 Polyclonal Antibody Human,Mouse,Rat WB,IHC
E-AB-63329 KO Validated NCOA2 Polyclonal Antibody Human,Mouse,Rat WB,IHC
E-AB-60188 KO Validated BCL10 Polyclonal Antibody Human WB
E-AB-63477 KO Validated EZH2 Polyclonal Antibody Human,Mouse,Rat WB,IF
E-AB-60192 KO Validated ANXA1 Polyclonal Antibody Human,Mouse WB,IHC,IF
E-AB-63539 KO Validated SQSTM1 / p62 Polyclonal Antibody Human,Mouse,Rat WB,IHC,IF
E-AB-63557 KO Validated EGFR Polyclonal Antibody Human,Mouse,Rat WB,IF
E-AB-60221 KO Validated HNRNPA2B1 Polyclonal Antibody Human,Mouse,Rat WB,IHC,IF
E-AB-62227 KO Validated STAT3 Polyclonal Antibody Human,Mouse,Rat WB,IF
E-AB-63601 KO Validated Vimentin Polyclonal Antibody Human,Mouse,Rat WB,IHC,IF
E-AB-63606 KO Validated BAX Polyclonal Antibody Human,Mouse,Rat WB,IHC
E-AB-64301 KO Validated MAP2K1 Polyclonal Antibody Human,Mouse,Rat WB,IHC
E-AB-64354 KO Validated NDUFS2 Polyclonal Antibody Human,Mouse,Rat WB,IF

Acetylation is related with protein activity, therefore the activity of target protein can be reflected through the specific identification of acetylated target protein by acetylated antibodies, and to study the function and regulation of protein.

Phosphorylation antibodies can identify the phosphorylation or non-phosphorylation of protein, and can be applied in qualitative and quantitative analysis of phosphorylated protein under the condition of avoid radiation pollution. Elabscience’s specific phosphorylated antibodies can be widely used in applications such as Western blot, Immunohischemistry, ELISA, etc.

Methylation is an important modification of proteins and nucleic acids, which can regulate the expression and silence of gene and is closely associated with cancer, aging, Alzheimer’s disease and other diseases, is one of the most important research content of epigenetics. The most common methylation modification includes DNA methylation and histone methylation.

Learn about the diverse collection of Acetyl/Phospho/Methyl Antibody in Elabscience and know how they can meet the needs of your researches.

Elabscience® Ready-to-Use Antibodies for IHC are high-quality monoclonal antibody, which has been repeatedly verified by the immunohistochemistry experiment of positive samples. These antibodies are mainly used for the identification, characterization and localization of intracellular or extracellular proteins. They are tested strictly to ensure the Ready-to-Use effect of the product and are cost-effective. This product is suitable for all kinds of immunohistochemistry kits produced by various manufacturers. It can be operated on different immunohistochemistry experimental instruments to avoid false negative caused by improper dilution of pre experimental antibody and save your precious samples and experimental time.

Elabscience® is committed to improve the quality and variety of antibodies to speed up your research process. Those products are 100% guaranteed , please feel free to consult and purchase.

Featured Elabscience® Ready-to-Use Antibodies for IHC

  • High Quality Monoclonal Antibody
  • Ready-to-Use, Easy to Operate
  • Tested Strictly
  • Store at 4°C for One Year

Display of Partial Ready-to-Use Antibodies for IHC

Kits

SARS-CoV-2 is a virus that can cause severe acute respiratory failure and spread on a large scale in the population. Its existence and transmission pose a serious threat to human life and physical health, and it has caused the most serious public health incident in the world in recent years.

SARS-CoV-2 has several structural proteins including spike (S), envelope (E), membrane (M) and nucleocapsid (N). The spike protein (S) contains a receptor-binding domain (RBD), which is responsible for recognizing the cell surface receptor, angiotensin converting enzyme-2 (ACE2). It is found that the RBD of the SARS-CoV-2 S protein strongly interacts with the human ACE2 receptor leading to endocytosis into the host cells of the deep lung and viral replication.

To effectively and quickly screen out SARS-CoV-2 asymptomatic, severely ill patients and recovered patients among large-scale populations, the application of molecular biology and immunology technology is particularly important. In order to help the SARS-CoV-2 research, Elabscience has developed 9 types of SARS-CoV-2 related antigen and antibody ELISA kits, which can meet customer research needs.

ELISART-PCRColloidal Gold Immunolateral Chromatography
Sample throughputVery high (hundreds of samples tested at one time)High (dozens of samples tested at one time)Low (one sample tested at a time)
Sample pre-processingLow (Centrifugal processing)High (Separation and purification, etc.)Low
Samples typesSerum, plasma, secretions, etc.Nasopharyngeal swabSerum, plasma, whole blood, etc.
Test sensitivityHigh (Detectable ng level)Low (Detectable μg level)
Test accuracyVery highHigh(Depending on sampling technique)High
Test repeatabilityHighHighNormal
Test time1-3.5 h1.5-2 h10-30 min
Requirements for experimental equipmentLow (Microplate Reader)Very high (sample extraction reagent, PCR apparatus, etc.)Low (visual interpretation)
Requirements for the level of laboratoryNormalVery high (Level P3 and above)Normal

ELISA kit for SARS-CoV-2 neutralizing antibody detection:

The neutralizing antibody detection is mainly for the detection of neutralizing epitope, spike protein S1 RBD antibodies to SARS-CoV-2, which aims to test whether recovered patients and vaccinators have immunity to SARS-CoV-2.

Product Name Cat. No. Target Sample Type Determination Method
SARS-CoV-2 Neutralization Antibody ELISA Kit E-EL-E606 Neutralization Antibody Human serum or plasma Qualitative Competitive
SARS-CoV-2 Neutralization Antibody ELISA Kit E-EL-E608 Neutralization Antibody Human serum or plasma Semi-Quantitative Competitive

ELISA kits for SARS-CoV-2 antigen detection:

Antigen detection includes the quantitative detection of SARS-CoV-2 spike protein S1 RBD and SARS-CoV-2 nucleocapsid protein, which is mainly used for screening asymptomatic infections and patients with mild or severe illness.

Product Name Cat. No. Target Sample Type Detection Range Sensitivity Determination Method
SARS-CoV-2 Spike Protein S1 RBD ELISA Kit E-EL-E605 S1 RBD serum, plasma and other biological fluids 0.39-25 ng/mL 0.09ng/mL Quantitative Sandwich-Ab
SARS-CoV-2 Nucleocapsid Protein ELISA Kit E-EL-E604 Nucleocapsid serum, plasma and other biological fluids 0.39-25 ng/mL 0.12ng/mL Quantitative Sandwich-Ab

ELISA Kits for SARS-CoV-2 antibody detection:

Antibody detection includes five types: SARS-CoV-2 spike protein IgM, SARS-CoV-2 spike protein IgG, SARS-CoV-2 spike protein total antibody, SARS-CoV-2 nucleocapsid protein IgM, SARS-CoV-2 nucleocapsid protein IgG. The qualitative detection of antibodies is mainly used for screening asymptomatic infections, mild and severe patients, and recovered patients, especially the spike protein total antibody test, which can screen patients at different stages of infection and minimize false negatives cases.

Product Name Cat. No. Target Sample Type Determination Method
SARS-CoV-2 Spike Protein IgM ELISA Kit E-EL-E603 Spike Protein IgM Human serum or plasma Qualitative Indirect
SARS-CoV-2 Spike Protein IgG ELISA Kit E-EL-E602 Spike Protein IgG Human serum or plasma Qualitative Indirect
SARS-CoV-2 Spike Protein Total Antibody ELISA Kit E-EL-E607 Spike Protein Total Antibody Serum, plasma or secretions Qualitative Sandwich-Ag
SARS-CoV-2 Nucleocapsid Protein IgM ELISA Kit E-EL-E601 Nucleocapsid Protein IgM Human serum or plasma Qualitative Indirect
SARS-CoV-2 Nucleocapsid Protein IgG ELISA Kit E-EL-E600 Nucleocapsid Protein IgG Human serum or plasma Qualitative Indirect

Focus on Developing and Manufacturing ELISA Assay Kits Set for Your Academic Research

Elabscience offers thousands of enzyme-linked immunosorbent assay (ELISA) kits for researchers, The species include human, mouse, rat, rabbit, monkey, porcine, etc. As a professional ELISA kits manufacturer and supplier, our ELISA test kits have been strictly quality-controlled to ensure the accuracy of results. Elabscience is committed to providing high quality ELISA kits with a very economical price. We always take the “customer oriented”, provide the best and professional services to customers. You can contact our distributors all around the world for purchase, or you can also buy our ELISA kits online directly.

QuicKey ELISA®

QuicKey ELISA® kit developed by Elabscience is an improvement of the traditional sandwich ELISA kit. It simplifies the operation process, saves at least 1h and requires less sample volume for assays, greatly improves the detection effciency.

QuicKey ELISA

Sandwich ELISA

Sandwich ELISA (or sandwich immunoassay) is the most commonly used elisa method. This format requires two antibodies/antigens specific for different epitopes of the antigen/antibodies. These two antibodies/antigens are normally referred to as matched antibody/antigen pairs. One of the antibodies/antigen is coated on the surface of the micro plate and used as the capture antibody/antigen to immobilize the tested analyte. The other antibody/antigen is conjugated with biotin or other markers to facilitate the detection of the analyte.

Sandwich ELISA

Competitive ELISA

Generally, Competitive ELISA (or competitive immunoassay) is applied for small molecular detection. The sample antigen competes with the pre-coated antigen for binding to a specific amount of labeled antibody. This assay only requires a single antibody conjugated to biotin commonly to facilitate the detection of the analyte.

Competitive ELISA

Elabscience® ELISA Kits Cited In Publications

Targets Products
8-OHdG [E-EL-0028]
Targets Products
CGRP1 [E-EL-R0135]
Targets Products
IL-8 [E-EL-H6008]
TNF-α [E-EL-H0109]
IL-1β [E-EL-H0149]
Targets Products
TGF-β1 [E-EL-0162]
IL-2 [E-EL-M0042]
IFN-γ [E-EL-M0048]
TNF-α [E-EL-M0049]
IL-12 [E-EL-M0726]

Sandwich ELISA Operation Guide Video

Food safety is an increasingly concerned problem all around the world. In order to ensure the safety of food and feed, more than 100 countries have formulated food and feed related regulations. Elabscience® is a global supplier of food and feed safety solutions. We provide a wide range of innovative testing solutions and services for qualitative and quantitative detection of mycotoxins, residues and contaminants in food and feed.

Test Methods - ELISA

Introduction

The lateral flow assay kit is rapid detection cards using immunogold gold technology. The test method is based on antigen-antibody reaction. The liquid sample droplets are added to the sample hole. If the target substance exists, a color zone will be seen on the test line (T) in 8 minutes. This visual evaluation enables qualitative analysis. This rapid detection card of immunogold gold technology is easy to operate and low cost.

Advantages of Lateral Flow

  • Rapid judgment of results
  • No laboratory equipment required
  • Suitable for field analysis
  • High stability
  • Free of harmful substances
  • No training costs

Test Methods - Lateral Flow

Introduction

Enzyme-linked immunosorbent assay established a quantitative ELISA method based on the selectivity and specificity of antibody and antigen recognition. The main principle of ELISA: one of these immune components is fixed in the holes of the solid phase (enzyme plate), and the detected target in the sample interacts with the antibody-antigen system. This interaction can be observed by enzymes in enzyme markers and indicate whether antibody-antigen binding occurs. The added substrate is transformed by coupling enzyme, resulting in color change, which can be measured by Microplate Reader.

Advantages of ELISA

  • High sensitivity: ppb(ng/mL) level
  • Quantitative analysis and no radioisotope pollution
  • Low batch-to-batch variation
  • Good specificity
  • Long valid period
  • Suitable for multiple samples

Elabscience® metabolism assay kits has a complete set of indicators and a wide range of applications, including Enzymes, Oxidative stress, Liver biomarkers, Kidney biomarkers, Amino acids & proteins, Glycolysis & Carbohydrates, Inorganic ions, Lipids metabolism, Plant stress resistance, Tricarboxylic Acid(TCA)Cycle, etc. Our assay kits suitable for a variety of sample types and the commonly used detection instruments which have spectrophotometer, microplate reader, fluorescence microplate reader, flow cytometer. Elabscience® metabolism assay kits has experienced 5 years of painstaking scientific research to create exclusive quality, and sold to more than 100 countries around the world. You can contact our distributors all around the world for purchase, or you can also buy our assay kits online directly.

Sample Types

Humor

  • Serum
  • Urine
  • Saliva

Tissue

  • Muscle
  • Liver Tissue
  • Pathological Tissue

Food

  • Juice
  • Honey
  • Milk

Others

  • Drug
  • Botany
  • Cosmetics

Immunolabelling technology is an immunobiochemistry analysis method to identify and determinate the trace and ultra-trace bioactive substances. The principle of immunolabeling is to covalently combine the marker (such as fluorescein, radioactive isotope, enzyme, biotin, etc.) with another compound, and then a multicomponent complex will form due to the specific reaction between the marked compound and tested compound. After the separation of unbound marked compound, you can observe and detect the experiment result with kinds of precise detection instruments.

The immunolabeling technology can be applied to take qualitative, quantitative or semi-quantitative analysis of samples, and it can also be used to study the location in cells or tissues. Elabscience® offers Fluorescence Labeling Kits and Biotin Labeling Kits with high-quality for researchers. Browse all Labeling Kits in below table.

Type Product name Reactive group Experimental time
Fuorescence labeling CY3 Labeling Kit CY3 100 min
FITC Labeling Kit FITC 100 min
Biotin labeling Biotin Labeling Kit NH2-Reactive Biotin 90 min
Long-arm Biotin Labeling Kit NHS-LC-LC-Biotin 90 min
Water-soluble Biotin Labeling Kit Sulfo-NHS-Biotin 90 min
Water-soluble Long-arm Biotin Labeling Kit Sulfo-NHS-LC-LC-Biotin 90 min

Cell Products

Complete Growth Medium
Universal complete growth medium
Primary cells complete growth medium
Cell lines complete growth medium
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Supplements & Reagents
Dissociation reagent
Antibiotics
Buffer & balance salt
Growth additive
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Cell culture is fundamental experimental techniques to life science, widely used in scientific research and industrial production. Elabscience provide classical basic medium, complete growth medium for primary cells and cell lines, and other supplements & reagents to meet the needs of the researchers.

Apoptosis is a complex and active death, involving the activation, expression and regulation of a series of genes.

When apoptosis occurs, a series of apoptotic processes are programmed, such as phosphatidylserines valgus outside of the cell membrane, mitochondrial membrane ΔΨm changes, caspase activation, DNA fragmentation and so on.

Different methods of apoptosis research

Apoptotic event line

Abnormal apoptotic mechanism can cause cells to lose control, which is closely related to the occurrence and development of many diseases such as tumors. Accurate detection of apoptotic cells is essential in the apoptosis study.

Elabscience® Cell Apoptosis Assay Products

Elabscience® has successfully developed multiple apoptosis detection products based on the most commonly used principles.

DNA Fragmentation

When cells undergo apoptosis, a large number of viscous 3′-OH are produced by breakage of chromosomal DNA. Under the action of deoxyribonucleotide terminal transferase (TdT), the deoxyribonucleotides and derivatives of fluorescein, peroxidase, alkaline phosphatase or biotin can be labeled to the 3′-terminal of DNA, so that apoptotic cells can be detected.

Caspase Activacion

Caspase family plays a very important role in the process of cell apoptosis. Caspase-3 is a key executive molecule, which plays a role in many pathways of apoptotic signal transduction. Caspase exists in the cytoplasm in the form of zymogen and in the early stage of apoptosis, the caspase is activated which eventually leads to apoptosis.

Phosphatidylserine Extemalization

It occurs at early apoptosis and runs through the whole cell apoptosis. Annexin V can bind to valgus phosphatidylserine under the action of Ca2+. Annexin V with DNA dyes such as PI and 7-AAD can distinguish living cells, early apoptotic cells, late apoptotic cells and dead cells.

Decrease of Mitochondrial Transmembrane Potential

The decrease of mitochondrial membrane potential is considered to be the earliest event in process of cell apoptosis. JC-1 dye exhibits different fluorescence emission due to different mitochondrial membrane potential. Cell apoptosis can be detected by detecting the change of cell membrane potential by JC-1 emission light.

Introduction

Elabscience® Caspase 3 Activity Assay Kit can detect the activity of Caspase 3 in cells or tissue lysis.

Detection principle

Caspase (Cysteine-requiring Aspartate Protease) is a protease family that plays an important role in the process of apoptosis. Caspase 3 (also known as CPP32, Yama, or apopain) is a member of the CED-3 subfamily of Caspases and is one of the critical enzymes of apoptosis. Caspase 3 is the most studied Caspase in mammalian cells. Caspase 3 can be used to cut proCaspase2, 6, 7, 9, and cut specifically many substrates of Caspase directly, including PPAR (poly ADP-ribose), the inhibitor of Caspase-activated deoxyribonuclease (ICAD), gelsolin and fodrin. Protein shearing mediated by Caspase 3 are important parts of the molecular mechanism of apoptosis. In addition, Caspase 3 also plays a key role in the process of nuclear apoptosis including chromatin condensation and DNA fragmentation as well as cell blebbing. Caspase 3 exists in the form of prozyme in the normal state and has no activity. However, during the apoptosis stage, activated Caspase 3 consists by two large subunits and two small subunits, which cleavages the corresponding substrate of endochylema or cytoplasmic nuclear and eventually leads to apoptosis.

This kit is used to conjugate Caspase 3 sequence-specific peptides acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) to yellow group p-nitroaniline (pNA). When the substrate is cut by Caspase 3, the yellow group pNA is dissociated. pNA has an absorption peak at 405 nm. Measure the OD value at 405 nm and then

Caspase 3 activity can be calculated accordingly.

Components

Cat. Products 20 Assays 50 Assays 100 Assays Storage
E-CK-A311A   Lysis Buffer 3.0 mL 7.5 mL 15mL -20°C
E-CK-A311B   2 ×Reaction Buffer 1.0 mL 1.25 mLx2 5.0 mL -20°C
E-CK-A311C   Ac-DEVD-pNA 100 μL 250 μL 500 μL -20°C
E-CK-A311D   DTT 50 μL 100 μL 150 μL -20°C
Manual  One Copy

Reagents not included

PBS, Protein Quantitative Kit (Bradford, optional).

Storage

Store at -20°C. Ac-DEVD-pNA[E-CK-A311C] should be stored in dark. Avoid freeze / thaw cycles.

Note

  1. For maximal assay performance, this reagent should be used within 12 months. Avoid freeze / thaw cycles.
  2. There is reducing agent (DTT) in the Lysis Buffer,It is recommended toElabscience® Total Protein (TP) Colorimetric Assay Kit (Coomassie Brilliant Blue Method)(It is recommended to use Elabscience® E-BC-K168-S) to measure the protein concentration instead of BCA method.
  3. It has been reported that the activity of Caspase 3 can’t be detected in few types of apoptosis, which may be due to the existence of a mechanism independent of the activation of Caspase 3, and other signaling pathways in the mechanism of apoptosis need to be considered. In this case, there is no significant change in the activity of Caspase 3 by using this kit.
  4. Lysis Buffer in this kit can be used in other Caspase activity assay kits byElabscience®. And the protein sample can test other Caspase activity.
  5. pNA (4-nitroaniline) is toxic. Please be careful when operating, and pay attention to effective protection to avoid direct contact with human body or inhalation. pNA solidifies at lower temperatures and sticks to the bottom, wall or cap of centrifugal tube. It can be incubated in water bath at 20~25°Cfor a short time until it is completely melted.
  6. When the activity of Caspase 3 in the sample is very low. Confirm whether the phenomenon of apoptosis is obvious or not firstly. If the apoptosis is obvious and we confirm that Caspase can be activated, please adjust the time of apoptosis and find a time point which Caspase 3 activation is stronger. And then repeat the test.
  7. This kit is for research use only.For your safety and health, please wear lab clothes and gloves. Instructions should be followed strictly, changes of operation may result in unreliable results.

Cell proliferation is an important life characteristic of organisms which is used to replenish senile or dead cells in the body. MTT Assay Kit (Cell Proliferation) has been widely used to detect cell proliferation and cytotoxicity. In recent years, the CCK-8 method has gradually replaced the traditional MTT method because of its high sensitivity and non-radioactivity.

Elabscience® Enhanced CCK-8 kit is more sensitive and with wider linear for detecting the number of living cells in cell viability, proliferation or cytotoxicity test.

Why Choose Elabscience® Cell Counting Kit 8 (WST-8 / CCK8)?

  • High sensitivity
  • Wide linear and perfect reproducibility
  • Low toxicity, simple operation
  • Multiple applications, drug screening, tumor drug sensitivity etc.

Hela cells: 100 μL/well
CCK-8 solution: 10 μL/well
Incubation: 1 h
Detection: Absorbance value (450 nm)

Protein Products

Recombinant protein is a manipulated form of protein produced through recombinant DNA technology. By inserting the DNA encoding the protein into bacterial or mammalian cells, we can get quantities of target proteins after amplifying expression and purification. Elabscience® offers a diverse selection of more than 4000 proteins for your research, including cytokines, growth factors, immune checkpoint proteins, CD antigen, FC Receptor, enzymes, hormone, etc.

Advantages of Elabscience® Recombinant Protein

Advantages of Elabscience<sup>® Recombinant Protein

Active Proteins

Elabscience® offers over 1000 different active proteins ready for experimental usage. There are a wide range of biological assay methods to measure the bioacitivity of a recombinant protein, including proliferation assay, cytokine induction assay, chemotaxis assay, cell proliferation assay, enzyme assay, funtional ELISA , etc. The recombinant proteins that validated bioactivity are available for cell culture, drug target research, candidate drugs, structural study, protein-protein interaction study and many other research fields.

Detection of Apoptotic Camptothecin-treated Jurkat by Annexin V Staining.

Jurkat were left untrated (left) or treated with 1 μM Camptothecin treated for 4 h (right) and then stained using Annexin V-APC. The combination of Annexin V(PKSH033460), APC labeled allows for the distinction between apoptotic cells (Annexin V-APC positive) and viable cells (unstained).

Recombinant human IL-6(PKSH033611) was measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 20-100pg/ml.

Cytokines

Elabscience® Cytokines are small secreted proteins released by immune cells that serve to regulate the immune system, which involve in autocrine, paracrine and endocrine signaling, also regulate the maturation, growth and responsiveness of various cell types. Elabscience® provides a comprehensive catalog of recombinant cytokines with excellent lot-to-lot consistency, superior activity and significantly low endotoxin levels, including Chemokines, Interferons(IFNs), Interleukins, and Tumour Necrosis Factors(TNFs), Cylony Stimulating Factors(CSFs), Growth Factors and Other Cytokine-Related Proteins.

Immune Checkpoint Proteins

Immune checkpoints are regulators of the immune activation. They consist of stimulatory and inhibitory pathways that maintain self-tolerance and assist with immune response. Stimulatory Immune checkpoints augment immunological responses against pathogens. On the contrary, inhibitory Immune checkpoints negatively regulate T-cell activation. In cancer, immune checkpoint mechanisms are often activated to suppress the nascent anti-tumor immune response, and PD-1, PD-L1, CTLA-4, CD28, OX40 are hot targets for cancer immunotherapy.

Chromatography is a most frequently used laboratory technique for separation and purification of biomolecules mixture. The mixture is dissolved in the mobile phase (a kind of fluid), which carries it through the stationary phase (a structure holding another material). The separation is based on differential partitioning between the mobile and stationary phases.

According to the different separation mechanism and principle, chromatography techniques are classified into different types for separation and purification process of the various target biomolecules. There are 4 types of commonly used chromatography techniques: Affinity chromatography, Ion exchange chromatography (IEC), Gel filtration (also known as Size-exclusion chromatography (SEC) and molecular sieve chromatography), and Hydrophobic interaction chromatography (HIC).

As a professional biotechnology company devoted to provide the best reagents and consumables for scientists, Elabscience® is committed to expand our product line and more high-quality products and services for scientific research. We have collected the most popular and acclaimed chromatographic media in one page. View and discover the enjoyable products!

Affinity chromatography

Affinity chromatography is a powerful chromatography technique of separating and purifying proteins based on the highly specific interaction between antigen and antibody, enzyme and substrate, or receptor and ligand. Such interactions including hydrogen bonding, ionic interaction, disulfide bridges, hydrophobic interaction, etc. Affinity chromatography are widely recognized for their high selectivity, high resolution and high capacity.

Ion exchange chromatography

Ion exchange chromatography (IEX) is a practical chromatography process that separates ions and polar molecules based on their respective charged groups. It works on almost any kind of charged molecule—including large proteins, small nucleotides, and amino acids.

Gel filtration chromatography

Gel filtration chromatography, also known as size exclusion chromatography (SEC), is a chromatographic method that separates molecules by their size or molecular weight. SEC can be used for wide ranges of separation and purification of components with large molecular weight differences and low resolution requirement, such as proteins, polysaccharides and other macromolecules.

Hydrophobic interaction chromatography

HIC is a useful method for purification and separation of biomolecules based on their surface hydrophobicity. HIC can be applied in the separation and purification of hydrophobic proteins such as aromatic and aliphatic compounds. In HIC, the matrix material is lightly substituted with hydrophobic groups, such as methyl, ethyl, propyl, octyl, or phenyl groups.

Name Code
Proteinase K Reagent E-IR-R109
Proteinase K Lyophilized Powder E-IR-R109U

 

Introduction

Proteinase K is a kind of high activity Proteinase of subtilisin, which is used to degrade proteins in biological samples. It can be used to digest various proteins, and it can be used in a variety of molecular biology, cell biology and other related experiments, such as genomic DNA extraction, enzyme digestion and removal, cell permeability and so on.

Enzyme activity, > 30 U/mg. At 37°C, the amount of Proteinase K that can produce amino acids or polypeptides equivalent to 1 micromol of tyrosine Folin positive in one minute with hemoglobin as the substrate is defined as a unit of Proteinase K activity.

The effective pH range of Proteinase K is pH4.0~12.5, and the optimal pH range is pH7.5~8.0.

The optimum reaction temperature of Proteinase K is 65°C, but at 65°C or higher, Proteinase K can also degrade rapidly. It is suggested that the optimum reaction temperature is 50~55°C.


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