Elabscience specialise in immunodiagnostic technologies for the life science community.

Their product range includes proteins, antibodies, ELISA kits, CLIA kits and labelling kits.

Every single product undergoes in house QC ensuring consistent and precise experimental results.

Elabscience products are sold in more than 100 countries worldwide.


Product Categories

Santa Cruz Alternatives

Elabscience has collected a detailed list of over 8,000 alternative Santa Cruz antibodies to secure your supply of antibodies and continuation of research.

If you cannot find your antibody on the list below please contact our technical team technical@stratech.co.uk who can help!

SCBT Cat. No. Target Elabscience Cat. Reactivity Application
sc-7122 BCL-x E-AB-40057 Human,Mouse,Rat WB,IHC
sc-6461 Cadherin-8 E-AB-40086 Human WB
sc-21578 CAMP E-AB-40068 Human,Mouse,Rat WB,IHC
sc-22169 CASP2 E-AB-40077 Human WB
sc-7056 CD46 E-AB-40059 Mouse,Rat IHC
sc-31191 CD48 E-AB-40042 Human,Rat IHC
sc-954 CDK1 E-AB-40071 Human WB
sc-163 CDK2 E-AB-40095 Human,Mouse WB,IHC
sc-17113 CK-20 E-AB-40067 Human,Mouse,Rat WB,IHC
sc-79916 COX5B E-AB-40083 Human,Mouse,Rat WB,IHC
sc-18304 CRP E-AB-40005 Rat WB,IHC
sc-16989 CST3 E-AB-40010 Rat WB,IHC
sc-31857 ENO1 E-AB-40064 Human,Mouse,Rat WB,IHC
sc-1310 EPO E-AB-40008 Rat IHC
sc-7888 IL10 E-AB-40072 Mouse,Rat IHC
sc-48544 IL2 E-AB-40026 Mouse,Rat IHC
sc-323975 IL6 E-AB-40073 Mouse,Rat IHC
sc-48408 Lep E-AB-40003 Rat IHC
sc-8196 MYD88 E-AB-40054 Human WB
sc-471 P21 E-AB-40097 Human WB
sc-1985 PPARA E-AB-40069 Human WB
sc-65598 Proliferating Cell Nuclear Antigen E-AB-40056 Human,Mouse,Rat WB,IHC
sc-27992 SDHA E-AB-40089 Human,Rat WB,IHC
sc-8332 SMAD3 E-AB-40050 Human,Rat WB,IHC
sc-53607 STAT1 E-AB-40052 Human,Rat WB,IHC
sc-34543 TGM2 E-AB-40088 Human,Mouse WB
sc-7814 TSHB E-AB-40009 Mouse,Rat WB,IHC
sc-79036 UQCRFS1 E-AB-40082 Human,Mouse WB,IHC
sc-152 VEGFA E-AB-40004 Rat WB,IHC

Protocols

The Elabscience® Protocols consist of collection of guidelines and frequently asked questions about the most commonly used immunology experiments. We built this forum to create a platform that can be used as reference for practical scientific experiments. We will continue to update and provide more technical information and practical resources.

Product Videos

Antibody WB Operation Guide Video

Sandwich ELISA Operation Guide Video

Antibody IHC Operation Guide Video

Elabscience Company Introduction Video

FAQs for Labeling Kit

Are there any cautions for the label?

 The label should contain free primary amines or N-terminal, and the sample should not contain Tris, glycerin, and reagents with amino.

Could this biotin labeling kit be used for labeling other proteins in addition to antibodies?

 The principle of biotin labeling kit is the formation of stable amide bond between activated Biotin and amino of label. In addition, N-terminal of protein can be labeled. Protein contains primary amines, such as lysine, can be labeled.

When labeling protein with this kit, does the reaction ratio is the same proportion of 20:1 as labeling antibodies?

 The molecular weight and lysine amount of protein should be considered. In general, it is recommended to label according to the concentration of 2 mg/mL. If the amount of lysine is about 10~15, the reaction ration can be 1:20. The proportion will be increased if the amount of lysine is more.

Can this kit be used for small or trace labeling?

 The assay procedure in the manual is used for labeling 0.1~2 mg antibody. If you need to mark a smaller amount, we have a special trace labeling method used to label 20-100 μg antibody and the reaction system is 100 μL. (So the amount of Biotin should be increased if the labeling concentration is less than 2 mg/mL.)

How to detect the labeling efficiency?

 The labeling kits on sale have their optimized labeling method. The labeling efficiency of these kits are generally above 90% from our test. In addition, antibodies in our ELISA kits are also labeled with Biotin labeling kit. The biotin labeling efficiency can be determined by detecting the biotin amount with our Biotin Detection Kit.

Can the Filtration tube in this kit be repeatedly used?

 The Filtration tube in this kit can be repeatedly used no more than 3 times, but must be washed after the assay finished use the following method: Add purified water and gently pipetting for several times. Then add 0.2M NaOH, incubate for 10 minutes and centrifuge. Wash the tubes with purified water. The tube can put in the water for short term storage, while it should be preserved in 20% ethanol for long term storage. The used tubes should avoid of dry and keep moist.

How long is the shelf life of the activated biotin after dissolution?

 The unopened freeze-dried biotin powder can be stored stably for 1 year at 4 ℃, and it can be stored for 4 weeks in the dark if sealed properly after opening/dissolving..

How to judge whether some specific substance (e.g., folic acid) can be labeled with FITC or not?

The target substance can be labeled if it contains free amine, and the amount of primary amine (lysine) should be considered for proteins. Small molecules can be determined according to the amino number. The containing nitrogen heterocyclic ring of folic acid contains 1 amine, so it can be labeled with FITC theoretically. But the amine on this site has a weak nucleophilicity, the labeling effect may not good and it should be judged according to the purpose of their experiment by customers.

Is the protein with a molecular weight of 3.8 kD suitable to be operated with a 3 kD Filtration tube?

 No. If your target protein has a molecular weight of 3.8 kD, you have to choose the Filtration tube with a blocked diameter of less than 3.8/3, otherwise there is possibility of skip or plugging aperture.

If my target protein has 43 lysine, but I just want to label less than 5 biotin molecules. Is it necessary to take quality control labeling? Are there any methods to verify the labeling effect and amount?

 Considering the spatial configuration, the reaction ratio optimized by Elabscience is 1:20, so that 1 antibody will be labeled with 3~6 Biotin molecules. If you want to control the labeling amount to be less than 5, you should control the molar ration and take pre-experiment, and estimate the labeling amount with Our Biotin Detetcion Kit.

FAQs for Antibodies

What concentration of primary antibody should I use?

For antibodies which the suggested working dilutions are listed as “Assay Dependent” this simply means that the optimal dilution should be obtained by the end user. However, we are always available to provide assistance.

Can I receive a free sample of a product?

 Generally, we do not offer free or trial sized samples for testing purposes. Our policy is that if an antibody does not work as specified on the datasheet, we will offer a replacement or refund (Except the promotion period. Detailed promotion information will be displayed on our website, or you can contact our sales). If the antibody is being used in an untested species or application, we cannot offer a replacement or refund.

How can I determine whether an antibody may detect in an untested species?

– Elabscience are unable to guarantee that an antibody will work in an untested species, even if the sequence alignment is high. There are many variables involved in the determining whether an antibody will bind in another species.

– If there are no alternatives available, and it is necessary for you to consider purchasing an antibody for use in a species that is not tested, we recommend checking the sequence alignment of the immunogen with the protein you are interested in.

Is the information on the datasheet up-to-date and correct?

 The datasheets contain the most up-to-date information we have available to us about this product. Where we find additional information, the datasheets are updated live on the website immediately,and you can contact our service team for any questions about Elabscience datasheet.

How should I choose a positive control?

 If you require a suitable positive control, please contact our technical support team and we would be happy to help. Additionally, make sure that the cell line or tissue being used is from one of the species listed on the data sheet.

What antigen retrieval method should I use with this antibody?

 We do not always have information available on the most suitable antigen retrieval method for an antibody in immunohistochemistry. This will often require a certain amount of optimization by the end user. Visit our IHC protocols page for more information on antigen retrieval methods.

FAQs for ELISA

Instrumentation sample preparation and storage for ELISA assays

How to deal with tissue or cells?

Normally, the amount and ratio of each reagent a fully automated microplate ELISA analyzer needs are not the same as in our kit, so it’s not applicable. Whereas if you can tell Cell lysates: For adherent cells, gently wash the cells with moderate amount of pre-cooled PBS and dissociate the cells by trypsin. Collect the cell suspension into the centrifugal tube and centrifuge for 5 minutes at 1000×g. Discard the medium and wash the cells for 3 times with pre-cooled PBS. For each 1×106 cells, add 150-250uL of pre-cooled PBS (0.01M, pH=7.4) to keep the cells resuspended. Repeat the freeze-thaw process for several times until the cells are lysed fully. Centrifuge for 10minutes at 1500×g at 2 – 8℃. Remove the cell fragments, collect the supernatant to carry out the assay. Avoid repeated freeze-thaw cycle.
Cell culture supernatant: Collect samples and centrifuge for 20 minutes at 1000×g at 2 – 8℃. Collect the supernatant to carry out the assay.
Tissue homogenates: Generally, mince the tissues to small pieces and rinse them in ice-cold PBS (0.01M, pH=7.4) to remove excess blood thoroughly. Tissue pieces should be weighed and then homogenized in PBS (tissue weight (g): PBS volume (mL) =1:9) with a glass homogenizer on ice. To further break the cells, you can sonicate the suspension with an ultrasonic cell disrupter or subject it to freeze-thaw cycles. The homogenates are then centrifuged for 5 minutes at 5000×g at 2 – 8℃, collect the supernatant to carry out the assay.Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g at 2 – 8℃ within 30 minutes of collection. Collect the supernatant to carry out the assay. Hemolysis samples are not suitable for ELISA assay.

How to collect serum and plasma?

Reading at dual wavelengths is to correct the optical density contributed by the plate wells and other nonspecific interference. Our plates are chosen for their optical quality, Serum: Allow samples to clot for 2 hours at room temperature or overnight at 4℃ before centrifugation for 15 minutes at 1000×g at 2 – 8℃. Collect the supernatant to carry out the assay. Blood collection tubes should be disposable, non-endotoxin.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g at 2 – 8℃ within 30 minutes of collection. Collect the supernatant to carry out the assay. Hemolysis samples are not suitable for ELISA assay.

Saliva, Urine, milk, tear and other types of biological fluids

In general, use a sterile container to collect fluids, then centrifuge it at 1000-5000×g at 2 – 8℃ for 5-20min to remove particulates, collect the supernatant to carry out the assay.

Storage

Samples should be assayed within 7 days when stored at 2-8℃, otherwise samples must be divided up and stored at -20℃ (≤1 month) or -80℃ (≤3 months). Avoid repeated freeze-thaw cycles. Centrifuge again before assaying to remove any additional precipitates that may appear after storage.

How to perform an ELISA assay as perfectly as possible?

How to calculate/ deal with the raw datas?

After correct the average OD values, plot a four parameter logistic curve on log-log graph paper, with standard concentration on the x-axis and OD values on the y-axis, many softwares could also help to deal with it, such as Origin( recommended), CurveExpert.

Target analyte in tested samples

 It’s recommended to test fresh samples by ELISA, which could avoid false positive or negative results caused by the degradation or decomposition of target analyte during long time storage.

Dilution factors and diluent

 Dilution ratio: Refer to the specific protocol or published papers, or consult to our technical support.

Recommended diluent: Standard & Sample Diluent provided in the ELISA kit, we could provide more vials of it for free for specific application, just tell us when you place the order.

Assay procedure

Following the protocol strictly, here are some hints
a. Establish the complete Standard Curve for each assay.
b. Ensure the precise volume for each reagent addition
c. Try to finish the liquid addition for each reagent as soon as possible, better not over 10 min.
d. Keep constant speed for liquid addition to each well
e. To get more accurate experiment result, it’s recommended to conduct the ELISA assay as soon as possible once you receive the kit.

FAQs for ELISA

PolyPeptide dissolution

Dissolving a polypeptide is a very complex process, and it is generally difficult to determine a suitable solvent quickly. Usually take a little for a preliminary test, and do not dissolve completely before determining the proper solvent.

The following methods can help you choose the right solvent:

 Determine the charge specificity of the polypeptide, set the acidic amino acid Asp (D), Glu (E) and C-terminal COOH to -1; basic amino acid Lys (K), Arg (R), His (H) and N-terminus NH2 is +1 and the charge of the other amino acids is zero. Calculate the net charge number.

 If the net charge number is >0, the polypeptide is alkaline and dissolved in water; if the peptide does not dissolve, add 10% acetic acid dropwise with vortexing. The peptide solution can also be warmed slightly. Longer peptides (over 20 amino acids) with a small overall net charge might require the addition of a stronger acid. Trifluoroacetic acid (TFA 10-50 µL) is often used to solubilize peptides but it is not cell-friendly and thus is used only when acetic acid fails to help solubilize the peptide. After the addition of TFA, the peptide should be diluted to approximately 1 mL with deionized water.

 If the net charge number is <0, the peptide is acidic and dissolved in water; If the peptide does not dissolve, add ammonium hydroxide (NH4OH 10-50 µL), and dilute the peptide to approximately 1 mL with deionized water.

Note: Caution must be used, however, with peptides that contain cysteine (C), as the used of alkaline pH can cause disulfide bond formation.

 If the net charge number = 0, the polypeptide is neutral and generally needs to be dissolved with an organic solvent such as acetonitrile, methanol or isopropanol, DMSO and the like. It has also been suggested that urea is required to dissolve highly hydrophobic polypeptides.

PolyPeptide preservation

Generally long-term preservation of polypeptides need to avoid light, and should be stored below -20 ℃, short-term can be stored at 4℃. It can be transported at room temperature for short periods of time. Polypeptides are very stable at -20℃, especially when they are lyophilized and stored in a desiccator. Before they are exposed to air, lyophilized polypeptides can be naturally heated up at room temperature, which reduces the effect of humidity. When lyophilizing is not possible, the best way is to store it in small amount of working samples.

For polypeptides containing Cys, Met or Trp, deoxidation buffers are essential for their dissolution, as the polypeptides are prone to air oxidation, and nitrogen or argon protection can reduce oxidation before sealing. Polypeptides containing Gln or Asn are more susceptible to degradation and have a shorter shelf life than normal peptides that do not contain these problematic amino acid.


Cell

Recommendations for handling of cryopreserved cells 1.   The cell is packaged by dry ice. When receiving the cell, please make Read More...

Biochemical Detection

Reaction principle of biochemical test kit 1 Reaction curve. Firstly, let's take a look at the time and absorbance of a Read More...

Flow Cytometric analysis

Detection of mitochondrial membrane potential Detection principle: The decrease of mitochondrial membrane potential is a marker event in the early Read More...

Labeling

FITC Labeling Kit Catalog Number: EBF0003 Introduction   The FITC Labeling Kit of Elabscience offering a collection of reagents required for Read More...

Immunofluorescence

IF

Immunofluorescence Guide Fixation and Permeabilization 1. Wash the cover glass seeded with cells in the culture plate with 1× PBS bufferfor Read More...

Immunohistochemical

IHC

Usage Method of Elabscience® RTU Antibody for IHC (Leica BOND MAX) Target This method is a guide to use the Read More...

Western Blot

WB

Comparison of Protein Separation Linear Range Concentration of separating gel Linear separation range 6% 50-150 kDa 8% 30-90 kDa 10% Read More...

Enzyme-Linked Immunosorbent Assay

ELISA

Common Problems about ELISA Problem Possible causes Solutions Poor precision Incomplete washing Ensure that the washing apparatus is working correctly; Read More...

Rapid Test Kits Development

Request a QuoteTechnical SupportPlace an Order Below is a selection of reagents for the development of COVID-19 rapid test kits. Read More...

COVID-19 Detection Kits

Request Rapid Test KitTechnical SupportRequest qPCR Test KitPlace an OrderAntibody Rapid Test Kits The antibody test kits detect the presence Read More...

elabscience covid-19

Research Solutions for Coronavirus – Elabscience

Contact usPlace an Order Ready-to-Use COVID-19 IgG/IgM Rapid Test KitCOVID-19 IgG/IgM Rapid Test Kit For suspicious patients with symptoms, mild Read More...

Coronavirus

COVID-19 Diagnostics Detection Kits (IgG/IgM Rapid test kits and RT-PCR)Viral Nucleic Acid Extraction Kits & Sample stabilisationCOVID-19 Research Products (Proteins, Read More...

VALIDATED BREAST CANCER ANTIBODIES

Breast Cancer Antibodies

Accurate protein expression verification method and high quality detection reagent are of great importance in promoting breast cancer research. Elabscience Read More…

First for Antibodies

Stratech Scientific have been successful in a recent antibodies tender from the Southern Universities Purchasing Consortium. In the award criterion Read More…