The conventional approach to commercial antibody production has improved remarkably since its inception well over 40 years ago. The development of reliable antibody reagents remains an essential goal in the quest to advance science and medicine. On the forefront of high-quality antibody manufacturing, GeneTex has solidified its position in the research community and the biotechnology industry.


Influenza
Influenza A and B Recombinant Monoclonal Antibodies
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Our experience and expertise can advance your research.

Let our team of antibody production experts help you develop a novel polyclonal or recombinant monoclonal antibody against your target of interest, with superior specificity, sensitivity, and application utility. GeneTex provides exceptional, comprehensive service that spans antigen preparation, animal immunization, antibody screening and purification, and recombinant cloning if desired. Our efficient workflow is guided by multiple production and performance checkpoints that are customized for each project, as are our pricing packages.

Contact us now to start developing your antibody.

Custom Rabbit Polyclonal Antibodies

Polyclonal antibodies are a heterogeneous mix of immunoglobulins derived from various B cells that individually target distinct epitopes on an immunogen. GeneTex has produced over ten thousand polyclonal antibodies in its history, resulting in exceptional manufacturing and quality control expertise that translates into reliable antibody reagents. Let GeneTex help you develop the ideal polyclonal antibodies to accelerate your research progress.

 

Key Design and Production Features

  1. Professional epitope design to optimize immunogen synthesis, solubility and antigenicity properties.
  2. Efficient production of high-quality peptide or protein immunogens.
  3. Enhanced cocktail immunization techniques.
  4. Antigen-affinity chromatography purification of antibodies.

 

Flow Chart for Polyclonal Antibody Production

Custom Polyclonal Antibody Development Packages

Package IPackage IIPackage III
Antigen preparationProteinPeptideModified peptide
Antigen designOOO
Protein expression and purificationO
Peptide synthesis OO
Peptide conjugation to carrier protein OO
ImmunizationOOO
Antigen-affinity antibody purificationOO
Antigen-affinity double-specific purification O
Turn-around time14~15 weeks14~15 weeks15~16 weeks

GeneTex’s recombinant antibody protocol involves cloning antibody-coding DNA from the peripheral blood B cells of an immunized rabbit or from a monoclonal hybridoma, employing an innovative technology to screen and identify antibodies that fit your specific application goals. Importantly, our FACS-based method preserves the natural pairing of the heavy and light chains from a single B cell or hybridoma cell, thereby maintaining the matched antibody variable regions. The entire procedure starting with FACS sorting requires only a few weeks. In addition, the generated recombinant antibody can be engineered into various Fc backbones, including rabbit, mouse, rat, or human IgG depending on your needs. And once cloned, your antibody is inexhaustible with exceptional reproducibility.

 

Flow Chart for Recombinant Antibody Production

Packages

Package IPackage IIPackage IIIPackage IV
Rabbit ImmunizationOO
Monoclonal Hybridoma OO
Screening and CloningOOOO
Pilot TestingOOOO
Antibody Mass Production O O

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Quality

With a 20-year history of quality results, GeneTex sets itself apart from other antibody companies by taking the necessary steps to properly validate our antibodies. Antibody validation remains an industry-wide challenge with ramifications that can impact progress in the life sciences and medicine.

At GeneTex, product reliability is our primary concern. Stringent validation protocols have been established to test our antibodies for various applications, including WB, IP, IHC, IFA, FACS, and ELISA, using cell lines with known target protein expression levels and a diverse array of tissue samples. We know the importance of replicating results, so quality consistency between lots is maintained confidently through a combination of pre-qualified individual lots, dominant clones, and thorough quality control.

Founded by scientists, for scientists, we at GeneTex understand that researchers expect and demand product performance and fidelity. With GeneTex’s Knockout/Knockdown validation project, we’re committed to demonstrating to our customers that the products they are purchasing are completely reliable, every single time.

Our Commitment

There has been growing distrust of commercially available antibodies with regard to specificity and performance variability. As a result, the research community has demanded that antibody suppliers increase their efforts to more thoroughly validate their products. One approach to address this is through knockout or knockdown of the target protein.

At GeneTex, we continue to provide the research community with the highest quality and most stringently validated antibodies by utilizing CRISPR/Cas9- and shRNA-generated cell lysates for our western blot assays. To date, we have evaluated most of our featured products and will continue to expand these efforts throughout our entire antibody catalogue.

GeneTex primary antibodies have been tested through the use of various analytic strategies to ensure persistent quality and confirm antibody specificity. Based on the guidelines described by the International Working Group on Antibody Validation (IWGAV), GeneTex has been employing multiple approaches to certify antibody performance in our quality assurance process.

 

See GeneTex’s Validation Methods
GeneTex understands the absolute necessity for reliable antibodies to achieve accurate and reproducible experimental results. To optimize the performance of our reagents, we employ various analytic validation strategies to ensure both consistent quality (see GeneTex’s Approach to Antibody Lot-to-Lot Variability) and specificity. These modalities are in line with guidelines described by the International Working Group on Antibody Validation (IWGAV) and have become fundamental components of our quality assurance process:

Products

Polyclonal antibodies generated by an immunized animal consist of a heterogeneous mix of immunoglobulins derived from many B-cells that individually target distinct epitopes on an antigen. Often representing up to 5% of the total immunoglobulin, these antibodies are generally purified from the immune sera using antigen-affinity chromatography. Polyclonal antibodies are particularly useful for detecting proteins that are expressed at lower levels, and their performance is impacted to a lesser degree by alterations in the antigen (e.g., denaturation). GeneTex offers an extensive catalogue of affinity-purified polyclonal antibodies directed against almost all key targets/proteins in the spectrum of biomedical research.

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Monoclonal antibodies are produced by immortal hybridoma cells created from the fusion of antibody-producing splenic B-cells and myeloma cells, or by recombinant protein expression in mammalian cells. These antibodies have monovalent affinity and bind to a single epitope on the antigen. Purification is commonly accomplished through Protein A/G/L affinity chromatography.

GeneTex offers a broad selection of monoclonal antibodies directed against the majority of key targets/proteins in the spectrum of biomedical research.

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Recombinant Antibodies

A recombinant antibody (rAb) is generated from the cloned, in vitro-expressed heavy and light chains of a selected monoclonal antibody obtained through classical hybridoma methodology, display strategies alone or in combination, sorted single B cells from immunized animals, or by one of many other techniques. The fact that it is cloned means the rAb is defined by its primary sequence, which can be engineered to optimize affinity and be expressed in different binder formats. The numerous advantages of rAbs have been well-documented, with consistency of performance being of paramount significance.

GeneTex’s rAb protocol employs a multi-parameter FACS-based approach to isolate antigen-specific IgG+ memory B cells from an immunized animal, with subsequent cloning of the antibody variable-region genes into an IgG backbone and expression in mammalian cells. This protocol is very rapid and can be completed in weeks, and also affords the opportunity to identify antibodies with diverse capabilities in various applications. Importantly, it allows cloning of the heavy and light chains from the same B cell, thereby preserving natural pairing. And once cloned, the supply of a given rAb is inexhaustible with exceptional reproducibility.

Recombinant Antibodies 2

One outstanding advantage of this protocol is that the suitability of the individual clones for desired applications can be tested during screening. For example, GeneTex’s Iba1 rabbit recombinant antibody [HL22] (GTX635363) detects Iba1, a protein commonly used as an immunohistochemical marker of both quiescent and activated microglia. In its development and production, both paraffin- and frozen-IHC analyses (IHC-P, IHC-Fr, respectively) were conducted in the first screening process to identify the best clones for these applications, as shown below (Figure 1). The clones were compared to a highly cited, market-leading commercial antibody to gauge their performance. This strategy provides us with valuable perspective on a clone’s market competitiveness during development. In addition, our recombinant antibody team has successfully expressed the antigen-binding regions of this antibody in the context of both mouse and rat IgG backbones, thus extending flexibility for multiple staining.

Iba1 antibody [HL22](GTX635363)

Figure 1. GeneTex’s recombinant rabbit monoclonal Iba1 antibody [HL22] (GTX635363) is superior to a competitor’s highly cited rabbit polyclonal antibody for both IHC-P (panels A vs. D) and IHC-Fr (panels B, C vs. E, F).

Another new rabbit recombinant product is GeneTex’s Ras (G12D mutant) antibody [HL10] (GTX635362), which specifically detects the G12D mutant by western blot with minimal background (Figure 2). Most importantly, it can also be used for IHC-P, where it demonstrates a robust signal and clear specificity for the G12D mutation in WT and KRAS G12D pancreatic tumor tissue sections (Figure 2A). This Ras (G12D mutant) antibody (GTX635362) is the first commercial recombinant version that shows exceptional specificity by IHC-P for this key tumorigenic mutant protein on sequence-verified human pancreatic tumor samples.

RAS (G12D Mutant) antibody [HL10](GTX635362)

Figure 2. (A) GeneTex’s recombinant rabbit RAS (G12D mutant) antibody [HL10] (GTX635362) is sensitive and specific for the RAS G12D mutation by IHC of a KRAS G12D mutant pancreatic tumor tissue section (top) compared to a wild-type KRAS section (bottom). (B) The antibody is sensitive and specific for the RAS G12D mutation by WB of extracts from wild-type and mutant KRAS-confirmed human pancreatic cell lines. GAPDH for the loading control was detected by GTX100118. Lane1: HPDE. Lane2: HPNE. Lane3: AsPC1 (KRAS G12D). Lane4: BxPC3 (KRAS WT). Lane5: CFPAC1 (KRAS G12V). Lane6: HPAC (KRAS G12D). Lane7: HPAF-II (KRAS G12D). Lane8: MIA PaCa-22 (KRAS G12C). Lane9: PANC1 (KRAS G12D). Lane10: SU86.86 (KRAS G12D).

As mentioned above, the antigen-binding regions of a recombinant antibody can be inserted into various host IgG backbones or expressed in different binder formats (e.g., Fab fragments or scFvs). Here, GeneTex’s cloned TSG101 mouse monoclonal antibody (GTX70255) was converted to a rabbit IgG backbone (GTX635396) with preservation of performance. No cross-reaction was observed when the converted TSG101 rabbit IgG recombinant antibody was used in combination with an anti-mouse IgG secondary antibody (Figure 3).

Figure 3. Switch from a cloned mouse monoclonal antibody to a rabbit IgG backbone

Secondary Antibody Panels

Name Catalogue No.
Anti-Rabbit IgG secondary antibody panel GTX300119
Anti-Mouse IgG secondary antibody panel GTX300120
EasyBlot Sampler Kit GTX300111

Other Isotype Controls

Name Catalogue No.
Chicken IgY isotype control GTX35001
Canine IgG isotype control GTX35072
Swine IgG isotype control GTX35046

Antigen Retrieval Reagents

Product Name Cat. No
Antigen retrieval condition-Pepsin (ready to use) GTX28194
Antigen retrieval solution GTX30709
Pronase reagent (Ready-to-use) GTX73181
Trypsin Antigen Retrieval Kit GTX30934
Antigen retrieval condition-Pepsin (ready to use) GTX30935
EDTA buffer, 10X pH 8.0 GTX30937
Citrate buffer, 10X pH6.0 GTX30936
Antigen Retrieval Solution GTX20970
Target Unmasking Fluid GTX30718

Counterstain Dyes

Product Name Code
DAPI Staining Solution GTX16206
Aqueous Hematoxylin (Ready-to-use) GTX73341
Nuclear Fast Red counterstain GTX73305
Light Green counterstain (Ready-to-use) GTX73306
Bisbenzimide / Hoechst 33342 GTX49386
Propidium Iodide GTX14083
Red aqueous counter stain™ GTX73304
Methylene Blue counterstain GTX73307
Haematoxylin GTX16203
Phalloidin (FITC) GTX49383
Eosin Y Counterstain (Ready-to-use) GTX73303
Eosin Y Counterstain (Ready-to-use) GTX73302

Detergents

Product Name Code
Tween 20 (50%) GTX30962
NP-40 (25%) GTX30961
Triton X-100 (25%) GTX30960
Brij 35 (Polyoxyethylene Laural Ether) GTX73318
Trident 20% SDS (w/v) GTX16370

Signal Enhancers

Product Name Code
SignalPlus Antibody Enhancer GTX49999
Signal+ for Immunostaining GTX49998
Peroxidase Enhancer GTX82979
Alkaline Phosphatase Enhancer GTX30944
Signal+ for Immunostaining – Solution A GTX10663
Signal+ for Immunostaining – Solution B GTX10666
AP Enhancer GTX20671

Mounting Media

Product Name Code
Fluoroshield™ with DAPI GTX30920
FluoroGel mounting medium GTX28214
ImmunoFluoroMount™ GTX30928
Clear Mount mounting medium- general purpose GTX30703
Clear Mount mounting medium-general purpose (Ready-to-use) GTX28213
Fluoroshield™ with DABCO GTX30927
Fluoroshield™ with PI GTX30921
Fluoroshield™ with Phenylenediamine GTX30926
Immuno Mount with DABCO™ GTX30930
Organo/Limonene Mount™ GTX30923
ImmunoHistoMount™ GTX30922
Immuno In Situ Mount™ GTX30924
Organo Mounting Medium (Ready-to-use) GTX78429

Chromogens

Product Name Code
Trident plus Western HRP Substrate GTX400006
Trident femto Western HRP Substrate GTX14698
AP chromogen BCIP/NBT (ready-to-use) GTX27468
DAB GTX30939
BCIP/NBT Substrate GTX30943
AEC Chromogenic kit GTX16204
Tetramethylbenzidine (TMB) substrate (Ready-to-use) GTX78422
DAB Chromogen/HRP Substrate kit GTX73338
AEC GTX30938
Fast Red Super™ GTX30942
DAB Enhancer GTX20675
Trident femto ELISA HRP substrate GTX00760
TMB-IHC (IHC,ICC,In Situ) GTX30947
3,3′,5,5′-Tetramethylbenzidine (TMB) Substrate, ELISA GTX14696
BCIP/NBT chromogen/substrate GTX73326
TMBM (For Membranes) GTX30946
TMB Substrate GTX30945

Blocking Reagents

Product Name Code
Trident Universal Protein Blocking Reagent (animal serum free) GTX30963
Blocking Solution with Donkey Serum (ready to use) GTX30972
Endogenous peroxidase blocking kit GTX30967
Blocking Solution with Goat Serum (ready to use) GTX30973
Streptavidin/Biotin blocking kit GTX30965
BSA (IgG and protease free) GTX30964
Blocking Solution with Rabbit Serum (ready to use) GTX30975
Blocking Solution with Bovine Serum (ready to use) GTX30970
Blocking Solution with Horse Serum (ready to use) GTX30974
Blocking Solution with Chicken Serum (ready to use) GTX30971
Blocking Buffer, PBS with BSA (10X) GTX48881
Avidin/Biotin blocking kit GTX30966
ImmunoStain blocker solution GTX73323
Blocking Solution with Fish Serum GTX85478
Blocking Buffer, TBS with BSA (10X) GTX48882
Blocking Buffer, TBS with 5% Milk GTX48883
Blocking Buffer, PBS with 1% Casein GTX48880
Blocking Buffer, TBS with 1% Casein GTX48879
Tissue conditioner GTX73312
Endo/Blocker GTX73347
ELISA Blocking Buffer GTX48884
Endogenous Alkaline phosphatase blocking kit GTX30968

Nucleotides / Plasmids

Name Code
easy iPSC GTX400001
easy iPSC + Antibody Sample Kit GTX400002
Methylated cytosine DNA standard kit GTX400004
RN18S1 (18S) Control Primer Pool GTX80116

EasyBlot Reagents and Kits

Your best choice for trouble-free IP-WB experiments

Immunoprecipitation (IP) and co-immunoprecipitation (co-IP) assays often necessitate the use of the same antibody, or antibodies from the same host, during both the IP and western blot (WB) procedures. Denatured immunoglobulin (Ig) in the IP sample can be bound by the secondary antibody used for the WB. Thus, depending on the molecular weight of the target protein, the desired band may be partially or completely obscured on the WB image by signal from denatured heavy (-50 kDa) and/or light (-25 kDa) Ig chains.
GeneTex’s EasyBlot® secondary antibodies are specifically optimized to eliminate this problem. They bind only the native (non-denatured and non-reduced) primary antibody used for WB analysis and not the denatured IP antibody chains. In addition, the specially formulated EasyBlocker reagent will greatly reduce background signal caused by free Protein A or Protein G.
EasyBlot®secondary antibodies are indispensable for achieving the cleanest results for your IP-WB experiments.

 

 

 

 

EasyBlot®: The easiest way to get the best result!

Clear
Efficiently eliminates denatured IgG masking of your target protein’s signal
Compatible
Suitable for WB following either IP or co-IP analysis using Protein A-, Protein G-, or Agarose-conjugated antibodies
Comprehensive
Broad range of EasyBlot® reagents for detecting various hosts: rabbit, mouse, rat, goat or sheep
Convenient: Simple and user-friendly!
Easy to switch from regular secondary antibodies to EasyBlot® secondary antibodies
Combine with EasyBlocker to further optimize your specific signal
Significantly reduce background generated by free Protein A/G with the EasyBlocker reagent.
Significantly reduce background generated by free Protein A/G with the EasyBlocker reagent.
Significantly reduce background generated by free Protein A/G with the EasyBlocker reagent.

 

 

Eliminate Product Name Cat. No Package
Masking bands by denatured IgG EasyBlot® anti-Rabbit IgG (HRP) GTX221666-01 50μl 250μl
EasyBlot® anti-Mouse IgG (HRP) GTX221667-01
EasyBlot® anti-Rat IgG (HRP) GTX628474-01
EasyBlot® anti-Goat IgG (HRP) GTX628547-01
EasyBlot® anti-Sheep IgG (HRP) GTX628906-01
Masking bands by denatured IgG & Background from protein A/G EasyBlot® anti-Rabbit IgG Kit (w/ EasyBlocker) GTX225856-01
EasyBlot® anti-Mouse IgG Kit (w/ EasyBlocker) GTX225857-01
EasyBlot® anti-Rat IgG Kit (w/ EasyBlocker) GTX228909-01
EasyBlot® anti-Goat IgG Kit (w/ EasyBlocker) GTX228910-01
EasyBlot® anti-Sheep IgG Kit (w/ EasyBlocker) GTX228911-01
Background from protein A/G EasyBlocker GTX425858 12g 62g

 

Product Name Cat. No Host & Clonality Application
Rabbit IgG isotype control GTX35035 Rb pAb Control, ELISA, FACS, ICC/IF, IHC, IHC-P, IP, WB, ChIP assay
Mouse IgG isotype control GTX35009 Ms pAb ELISA, FACS, ICC/IF, IP
Mouse IgG2a isotype control [HOPC-1] GTX35019 Ms mAb ELISA, FACS, ICC/IF, IHC-Fr, IP, WB, in vitro, FLISA, in vivo
Mouse IgG3 isotype control [B10] GTX35027 Ms mAb ELISA, FACS, ICC/IF, IP, in vitro, FLISA
Goat IgG isotype control GTX35039 Gt pAb ELISA, FACS, IHC, IHC-P, IP

Buy 1 Get 25ul Free!

Receive a FREE 25ul size antibody for every full-size primary antibody  More than 9,000 antibodies to select from!  Receive a Read More...

GeneTex FAQs

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The ALS-Reproducible Antibody Platform (ALS-RAP) Webinar

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Exosome Research

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Webinar PD-L1

Thank you for your interest in the seminar “Enhancing anti-cancer immune response by targeting glycosylated PD-L1". Objectives:  1. Immunity and Read More...

Epitope Tags & Reporters

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Loading Controls

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VetSignal

GeneTex is proud to launch its new VetSignal™ line of products to advance veterinary science research. These antibodies and other Read More...

Zebrafish

As the leading manufacturer of zebrafish antibodies, GeneTex offers an extensive portfolio of reagents validated for use in this model Read More...

Infectious Diseases

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Signaling Pathways

Cell signaling refers to the various sequences of molecular events that mark a cell’s response to its environment, whether that Read More...

Cell Markers

Cell markers are biochemical or genetic characteristics that distinguish and discriminate between different cell types. Although most are molecules expressed Read More...

Cardiovascular

The cardiovascular system is composed of the heart and circulatory system. It is responsible for delivery of oxygen- and nutrient-rich Read More...

Epigenetics

Epigenetics is the study of heritable phenotype changes that affect gene activity and expression that do not involve alterations in Read More...

Neuroscience

Neuroscience research has become a major focus for countries all over the world. The United States, European nations, Japan, China, Read More...

Tumor Markers

A tumor marker is a substance produced, or induced, by cancer cells that can be detected in body fluids or Read More...

Cancer

Oncoproteins The products of metazoan cell proto-oncogenes are generally key constituents of the cell signaling and gene expression machinery that Read More...

Necrosis

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