The conventional approach to commercial antibody production has improved remarkably since its inception well over 40 years ago. The development of reliable antibody reagents remains an essential goal in the quest to advance science and medicine. On the forefront of high-quality antibody manufacturing, GeneTex has solidified its position in the research community and the biotechnology industry.
With a 20-year history of quality results, GeneTex sets itself apart from other antibody companies by taking the necessary steps to properly validate our antibodies. Antibody validation remains an industry-wide challenge with ramifications that can impact progress in the life sciences and medicine.
At GeneTex, product reliability is our primary concern. Stringent validation protocols have been established to test our antibodies for various applications, including WB, IP, IHC, IFA, FACS, and ELISA, using cell lines with known target protein expression levels and a diverse array of tissue samples. We know the importance of replicating results, so quality consistency between lots is maintained confidently through a combination of pre-qualified individual lots, dominant clones, and thorough quality control.
There has been growing distrust of commercially available antibodies with regard to specificity and performance variability. As a result, the research community has demanded that antibody suppliers increase their efforts to more thoroughly validate their products. One approach to address this is through knockout or knockdown of the target protein.
At GeneTex, we continue to provide the research community with the highest quality and most stringently validated antibodies by utilizing CRISPR/Cas9- and shRNA-generated cell lysates for our western blot assays. To date, we have evaluated most of our featured products and will continue to expand these efforts throughout our entire antibody catalogue.
|GeneTex primary antibodies have been tested through the use of various analytic strategies to ensure persistent quality and confirm antibody specificity. Based on the guidelines described by the International Working Group on Antibody Validation (IWGAV), GeneTex has been employing multiple approaches to certify antibody performance in our quality assurance process.|
|See GeneTex’s Validation Methods|
|GeneTex understands the absolute necessity for reliable antibodies to achieve accurate and reproducible experimental results. To optimize the performance of our reagents, we employ various analytic validation strategies to ensure both consistent quality (see GeneTex’s Approach to Antibody Lot-to-Lot Variability) and specificity. These modalities are in line with guidelines described by the International Working Group on Antibody Validation (IWGAV) and have become fundamental components of our quality assurance process:|
Polyclonal antibodies generated by an immunized animal consist of a heterogeneous mix of immunoglobulins derived from many B-cells that individually target distinct epitopes on an antigen. Often representing up to 5% of the total immunoglobulin, these antibodies are generally purified from the immune sera using antigen-affinity chromatography. Polyclonal antibodies are particularly useful for detecting proteins that are expressed at lower levels, and their performance is impacted to a lesser degree by alterations in the antigen (e.g., denaturation). GeneTex offers an extensive catalog of affinity-purified polyclonal antibodies directed against almost all key targets/proteins in the spectrum of biomedical research.
|Monoclonal antibodies are produced by immortal hybridoma cells created from the fusion of antibody-producing splenic B-cells and myeloma cells, or by recombinant protein expression in mammalian cells. These antibodies have monovalent affinity and bind to a single epitope on the antigen. Purification is commonly accomplished through Protein A/G/L affinity chromatography.GeneTex offers a broad selection of monoclonal antibodies directed against the majority of key targets/proteins in the spectrum of biomedical research.|